Analysis of expressed sequence tags and the whole genome have shown a remarkable degree of similarity in the gene sequence ... Recent sequencing of its complex genome has shown that it has an estimated complement of 18,000 protein-coding genes. Its ... IUCN Red List vulnerable species, Articles with 'species' microformats, Edwardsiidae, Cnidarians of the Atlantic Ocean, ... Its genome has been sequenced and it is cultivated in the laboratory as a model organism, but the IUCN has listed it as being a ...

It is one of the several genes that appeared in the search in expressed sequence tag (EST) databases of mouse. It was then ... Homeobox protein NOBOX, also known as newborn ovary homeobox protein, is a protein that in humans is encoded by the NOBOX gene ... stretch of 32 specific amino acids in primates downstream the NOBOX Arg303 residue and is very well-conserved among the species ... Bayne RA, Kinnell HL, Coutts SM, He J, Childs AJ, Anderson RA (2015-03-19). "GDF9 is transiently expressed in oocytes before ...

... all species). EST approaches have largely been superseded by whole genome and transcriptome sequencing and metagenome ... Putney SD, Herlihy WC, Schimmel P (1983). "A new troponin T and cDNA clones for 13 different muscle proteins, found by shotgun ... In genetics, an expressed sequence tag (EST) is a short sub-sequence of a cDNA sequence. ESTs may be used to identify gene ... Because of the way ESTs are sequenced, many distinct expressed sequence tags are often partial sequences that correspond to the ...

There is no conclusive data regarding whether the gene is ubiquitously expressed in human tissues, but expressed sequence tag ... RsfS has been shown via a yeast two-hybrid screen to interact with ribosomal protein L14 in four bacterial species as well as ... The domain is from position 93 to 194 on the human protein and comprises 43.2% of the sequence. This conserved domain is also ... MALSU1 is a gene on chromosome 7 in humans that encodes the protein MALSU1. This protein localizes to mitochondria and is ...

Six-frame translations can utilize an expressed sequence tag (EST) to generate protein databases. EST data provide ... compares proteomic data from multiple related species concurrently and exploits the homology between their proteins to improve ... Protein databases can also be created by using RNA sequencing data, annotated RNA transcripts, and variant protein sequences. ... and proteins, proteomics relies on the assumption that current gene models are correct and that all relevant protein sequences ...

Both Northern blot analysis and inspection of the human expressed sequence tag (EST) database suggest relatively little ... As well, the sequence of MCT2 is far less conserved across species than that of MCT1 or MCT4 and there also appear to be ... Monocarboxylate transporter 2 (MCT2) also known as solute carrier family 16 member 7 (SLC16A7) is a protein that in humans is ... considerable species differences in the tissue expression profile of this isoform. Of the four known mammalian lactate ...

The Helminth Secretome Database (HSD) is a repository for helminth proteins predicted using expressed sequence tags (ESTs). ... A number of helminth species also secrete high levels of antioxidants to avoid phagocytosis; those antioxidants are needed ... a collection of helminth excretory/secretory proteins predicted from expressed sequence tags (ESTs)". BMC Genomics. 13 Suppl 7 ... Helminth proteins modulate the immune response of their hosts, but do not suppress it entirely. A number of proteins are able ...

As inferred from expressed sequence tag analysis, the average intron size found in D. pulex genes is 170 bp. The intron density ... "Reconstructing the phylogeny of 21 completely sequenced arthropod species based on their motor proteins". BMC Genomics. 10: 173 ... It is a model species, and was the first crustacean to have its genome sequenced. D. pulex is an arthropod whose body segments ... Daphnia pulex is the most common species of water flea. It has a cosmopolitan distribution: the species is found throughout the ...

"Adult midgut expressed sequence tags from the tsetse fly Glossina morsitans morsitans and expression analysis of putative ... Glossina morsitans is variously classified into the subgenus Glossina or as the name species of a morsitans species group. Note ... Protein - NCBI". NCBI (National Center for Biotechnology Information). v t e (Articles with short description, Short ... G. m. submorsitans is a common substitute for G. m. morsitans, or rarely as a full species G. submorsitans. G.m.s. is further ...

Expression data from Expressed Sequence Tag mapping, microarray and in situ hybridization show high expression for Homo sapiens ... "Statistical Analysis of Protein Sequence (Biology Workbench)".[permanent dead link] Meechan DW, Maynard TM, Tucker ES, LaMantia ... This domain is found among distantly related species from the six kingdoms: Eubacteria, Archaebacteria, Protista, Fungi, ... C22orf25 is also xenologous to T10 like proteins in the Fowlpox Virus and Canarypox Virus. The gene coding for C22orf25 is ...

Both expressed sequence tags and GEO profiles show the number of transcripts of a gene present in a certain tissue type and ... A comparison of predicted phosphorylation sites in human, mouse and zebrafish shows that all three species have approximately ... Protein FAM46B also known as family with sequence similarity 46 member B is a protein that in humans is encoded by the FAM46B ... A second protein shown to physically interact with FAM46B is DAZAP2, is a proline-rich brain expressed protein. In combination ...

Alignment of a protein or mRNA sequence from one species onto a sequence database from another species to determine homology. ... It was designed primarily to decrease the time needed to align millions of mouse genomic reads and expressed sequence tags ... provide step by step protocols for how to use BLAT to: Map an mRNA/cDNA sequence to a genomic sequence; Map a protein sequence ... In addition, protein or translated sequence queries are more effective for identifying distant matches and for cross-species ...

Diversity of odourant binding proteins revealed by an expressed sequence tag project on male Manduca sexta moth antennae. ... Sequence variation of Bemisia tabaci Chemosensory protein 2 in cryptic species B and Q: new DNA markers for whitefly ... bacteria and insect genome and Expressed Sequence Tag (EST) databases in the continuity of molecular data that demonstrate that ... Ma C, Cui S, Tian Z, Zhang Y, Chen G, Gao X, Tian Z, Chen H, Guo J, Zhou Z. OcomCSP12, a chemosensory protein expressed ...

In an Expressed Sequence Tag (EST) study conducted by Zhang and Yang in 2007 C. cupreum demonstrated a diverse expression of ... as well as glycoprotein and peptide-protein biosynthesis. Many genes have been identified in this species that support protein ... Zhang, HaiYan; Yang, Qian (13 January 2007). "Expressed sequence tags-based identification of genes in the biocontrol agent ... The species is distinguished from other Chaetomium species by a high frequency of boat-shaped ascospores and copper coloured ...

During the 1990s, expressed sequence tag sequencing was used to identify genes and their fragments. This was followed by ... glaucophyta and rhodophyta were sequenced. The protein coding sequences were subsequently compared to infer phylogenetic ... Since mRNA is the species of interest and it represents only 3% of its total content, the RNA sample should be treated to ... Initially, transcriptomes were analyzed and studied using expressed sequence tags libraries and serial and cap analysis of gene ...

If a new phenotype is observed, the genome is sequenced and searched for tagged transposons. If the site of transposon ... Possible mutations: Insertion in a translated region => hybrid protein/truncated protein. Usually causes loss of protein ... Only successful inserts of plasmids with the plasmid 'housekeeping' sequences will express this gene. 7. The gene can be cloned ... mariner has well conserved homologues across a wide range of species and is a very versatile tool. This article incorporates ...

March 2000). "Shotgun sequencing of the human transcriptome with ORF expressed sequence tags". Proceedings of the National ... Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ... 60S ribosomal protein L32 is a protein that in humans is encoded by the RPL32 gene. Ribosomes, the organelles that catalyze ... The protein belongs to the L32E family of ribosomal proteins. It is located in the cytoplasm. Although some studies have mapped ...

2000). "Shotgun sequencing of the human transcriptome with ORF expressed sequence tags". Proc. Natl. Acad. Sci. U.S.A. 97 (7): ... Probable DNA dC->dU-editing enzyme APOBEC-3D is a protein that in humans is encoded by the APOBEC3D gene. This gene is a member ... 2003). "Species-specific exclusion of APOBEC3G from HIV-1 virions by Vif". Cell. 114 (1): 21-31. doi:10.1016/S0092-8674(03) ... 1999). "The DNA sequence of human chromosome 22". Nature. 402 (6761): 489-95. Bibcode:1999Natur.402..489D. doi:10.1038/990031. ...

The enzyme responsible for the creation of the conjugated double bonds was identified using expressed sequence tags, and called ... "Affinity of phosphatidylcholine molecular species for the bovine phosphatidylcholine and phosphatidylinositol transfer proteins ... ISBN 0-8342-1342-7. Endo S, Zhiping G, Takagi T. (1991). Lipid components of seven species of Basidiomycotina and three species ... α-Parinaric acid has been used to study the hydrophobicity and foaming characteristics of food proteins, as well as the foam ...

1997). "Generation and analysis of 280,000 human expressed sequence tags". Genome Res. 6 (9): 807-28. doi:10.1101/gr.6.9.807. ... Bax inhibitor 1 is a protein that in humans is encoded by the TMBIM6 gene. GRCh38: Ensembl release 89: ENSG00000139644 - ... 2007). "Bax inhibitor-1 regulates endoplasmic reticulum stress-associated reactive oxygen species and heme oxygenase-1 ... 2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci ...

Based on human expressed sequence tag (EST) profiles, TMEM239 appears to be expressed in the testis and the brain. According to ... "An inter-species protein-protein interaction network across vast evolutionary distance". Molecular Systems Biology. 12 (4): 865 ... protein". www.uniprot.org. Retrieved 2016-04-25. "TAE1 - Alpha N-terminal protein methyltransferase 1 - Saccharomyces ... RhoGEF and PH domain-containing protein 2 (FGD2) and Syndecan binding protein (SDCBP). The Golgi SNAP receptor complex member 1 ...

Prokupek, A.; Hoffmann, F.; Eyun, S. I.; Moriyama, E.; Zhou, M.; Harshman, L. (2008). "An Evolutionary Expressed Sequence Tag ... as is thought to be the case in seminal proteins known as Acps in species of Drosophila melanogaster. While Acps facilitate the ... through genetic sequencing and analysis. It is hypothesized that these proteases break down various proteins in male seminal ... In this species females have coiled oviducts that lead to the spermatheca that in turn make it hard for males to reach the area ...

There are 7,006 identified expressed sequenced tags (ESTs), representing 2,564 genes. Clinical ostertagiosis normally occurs in ... Dietary energy and protein, which would otherwise be used for growth, must be used to replace these proteins. Weight loss is ... All species of the genus Ostertagia infect domestic or wild ruminants. These species form a large and complex group, the ... as it lacks the two refractive bodies in the head that is seen in this species. The tail of the larvae within the sheath is ...

These methods optimise the protein identification process by producing the DNA sequences of all proteins expressed in the venom ... The transcriptome gave a cDNA of over 1100 expressed sequence tags (ESTs), with only 688 sequences being related to the venom ... "Protein-species quantitative venomics: looking through a crystal ball". Journal of Venomous Animals and Toxins Including ... Conversely, snake venom is made up of more complex protein such as modified saliva proteins (CRISPs & kallikrein) and protein ...

For example, the RefSeq database contains transcript and protein sequence from many different species, and the Ensembl system ... target genome is searched for sequences that are similar to extrinsic evidence in the form of the known expressed sequence tags ... Given a protein sequence, a family of possible coding DNA sequences can be derived by reverse translation of the genetic code. ... List of gene prediction software Phylogenetic footprinting Protein function prediction Protein structure prediction Protein- ...

This sample-specific cell tagging allows pooling of the sequencing libraries prepared from different samples on a sequencing ... to evaluate both gene expression and protein levels simultaneously in different species. The method was established by the New ... uses oligonucleotide-labelled antibodies against ubiquitously expressed cell surface proteins from a particular tissue sample. ... Sequencing the antibody tags along with the cellular transcriptome helps identify a sample of origin for each analyzed cell. A ...

The project built on efforts to sequence expressed sequence tags from A. thaliana. Descriptions of the sequences of chromosomes ... discovered the PHD finger in the protein HAT3.1. It has since proven to be important to chromatin in a wide variety of taxa. ... Arabidopsis thaliana is a first class model organism and the single most important species for fundamental research in plant ... Direct sequencing of cDNA ("RNA-Seq") largely replaced microarray analysis of gene expression, and several studies sequenced ...

It has been estimated that ~100,000 expressed sequence tags (ESTs) can be identified in humans. Isoforms at the protein level ... Schlüter H, Apweiler R, Holzhütter HG, Jungblut PR (September 2009). "Finding one's way in proteomics: a protein species ... A protein isoform, or "protein variant", is a member of a set of highly similar proteins that originate from a single gene or ... Generally, one protein isoform is labeled as the canonical sequence based on criteria such as its prevalence and similarity to ...

... "expressed sequence tags". cDNA libraries are useful in reverse genetics, but they only represent a very small (less than 1%) ... The expressed proteins from these libraries can then be screened for variants which exhibit favorable properties (e.g. ... Additionally, for cDNA libraries, a system using the Lambda Zap II phage, ExAssist, and 2 E. coli species has been developed. A ... Determining the complete genome sequence of a given organism (see genome project) Serving as a source of genomic sequence for ...

During the 1990s there were guesstimates of up to 100,000 genes and early data on detection of mRNAs (expressed sequence tags) ... The nucleotide sequence of a gene's DNA specifies the amino acid sequence of a protein through the genetic code. Sets of three ... ISBN 978-0-19-286092-7. Ridley M (1999). Genome: The Autobiography of a Species in 23 Chapters. Fourth Estate. ISBN 978-0-00- ... The structure of a protein-coding gene consists of many elements of which the actual protein coding sequence is often only a ...