Immunostaining: Immunostaining is a general term in biochemistry that applies to any use of an antibody-based method to detect a specific protein in a sample. The term immunostaining was originally used to refer to the immunohistochemical staining of tissue sections, as first described by Albert Coons in 1941.Low-voltage electron microscope: Low-voltage electron microscope (LVEM) is an electron microscope which operates at accelerating voltages of a few kiloelectronvolts or less. While the low voltage electron microscopy technique will never replace conventional high voltage electron microscopes, it is quickly becoming appreciated for many different disciplines.TSOM: Through-Focus Scanning Optical Microscopy (TSOM) is an imaging method that produces nanometer-scale three-dimensional measurement sensitivity using a conventional bright-field optical microscope. TSOM has been introduced and maintained by Ravikiran Attotahttp://www.Field emission probesMauna Kea Technologies: Mauna Kea Technologies is a global medical device company focused on leading innovation in endomicroscopy, the field of microscopic imaging during endoscopy procedures. The company researches, develops and markets tools to visualize, detect and rule out abnormalities including malignant and pre-malignant tumors or lesions in the gastrointestinal and pulmonary tracts.Total internal reflection fluorescence microscope: A total internal reflection fluorescence microscope (TIRFM) is a type of microscope with which a thin region of a specimen, usually less than 200 nm can be observed.Lamellar granule: Lamellar granules (otherwise known as membrane-coating granules (MCGs), lamellar bodies, keratinosomes or Odland bodies) are secretory organelles found in type II pneumocytes and keratinocytes. They are oblong structures, appearing about 300-400 nm in width and 100-150 nm in length in transmission electron microscopy images.Nanosensors (company)Cell membraneMultiphoton fluorescence microscope: A multiphoton fluorescence microscope (MFM) is a specialized optical microscope.Monoclonal antibody therapyColes PhillipsElectron beam-induced deposition: Electron beam-induced deposition (EBID) is a process of decomposing gaseous molecules by electron beam leading to deposition of non-volatile fragments onto a nearby substrate. The electron beam is usually provided by a scanning electron microscope that results in high spatial accuracy (below one nanometer) and possibility to produce free-standing, three-dimensional structures.Membrane protein: Membrane proteins are proteins that interact with biological membranes. They are one of the common types of protein along with soluble globular proteins, fibrous proteins, and disordered proteins.Silicene: Silicene is a two-dimensional allotrope of silicon, with a hexagonal honeycomb structure similar to that of graphene.Effective circulating volume: Effective Circulating Volume (ECV) is the volume of arterial blood (vascular extracellular fluid) effectively perfusing tissue. ECV is a dynamic quantity and not a measurable, distinct compartment.Protein primary structure: The primary structure of a peptide or protein is the linear sequence of its amino acid structural units, and partly comprises its overall biomolecular structure. By convention, the primary structure of a protein is reported starting from the amino-terminal (N) end to the carboxyl-terminal (C) end.CisternaPolyvalent DNA gold nanoparticles: Polyvalent DNA gold nanoparticles are colloidal gold whose surface is modified with thiol capped synthetic DNA sequences. They were co-discovered by Chad Mirkin et al.Titin: Titin , also known as connectin, is a protein that, in humans, is encoded by the TTN gene. Titin is a giant protein, greater than 1 µm in length, that functions as a molecular spring which is responsible for the passive elasticity of muscle.Nanovid microscopy: Nanovid microscopy, from “nanometer video-enhanced microscopy”, is a microscopic technique aimed at visualizing colloidal gold particles of 20-40 nm diameter (nanogold, immunogold) as dynamic markers at the light microscopic level. The nanogold particles as such are smaller than the diffraction limit of light, but can be visualized by using Video-Enhanced Differential Interference Contrast (VEDIC).Specific granule: Specific granules are secretory vesicles found exclusively in cells of the immune system called granulocytes.Protoplasm: Protoplasm is the living content of a cell that is surrounded by a plasma membrane. It is a general term for the cytoplasm.Organelle biogenesis: Organelle biogenesis is the biogenesis, or creation, of cellular organelles in cells. Organelle biogenesis includes the process by which cellular organelles are split between daughter cells during mitosis; this process is called organelle inheritance.Vital stain: A vital stain in a casual usage may mean a stain that can be applied on living cells without killing them. Vital stains have been useful for diagnostic and surgical techniques in a variety of medical specialties.Live cell imaging: __NOTOC__Molar mass distribution: In linear polymers the individual polymer chains rarely have exactly the same degree of polymerization and molar mass, and there is always a distribution around an average value. The molar mass distribution (or molecular weight distribution) in a polymer describes the relationship between the number of moles of each polymer species (Ni) and the molar mass (Mi) of that species.New Zealand rabbitKASH domains: KASH domains are conserved C-terminal protein regions less than ~30 amino acids. KASH is an acronym for Klarsicht, ANC-1, Syne Homology.Immunoperoxidase: Immunoperoxidase is a type of immunostain used in molecular biology, medical research, and clinical diagnostics. In particular, immunoperoxidase reactions refer to a sub-class of immunohistochemical or immunocytochemical procedures in which the antibodies are visualized via a peroxidase-catalyzed reaction.Cryptic self epitopes: In immunology, cryptic self epitopes are a source of autoimmunity.Scanning probe microscopy: Scanning probe microscopy (SPM) is a branch of microscopy that forms images of surfaces using a physical probe that scans the specimen. SPM was founded in 1981, with the invention of the scanning tunneling microscope, an instrument for imaging surfaces at the atomic level.CytoskeletonGlomerular basement membrane: The glomerular basement membrane (GBM) of the kidney is the basal lamina layer of the glomerulus. The glomerular capillary endothelial cells, the GBM and the filtration slits between the podocytes perform the filtration function of the glomerulus, separating the blood in the capillaries from the filtrate that forms in Bowman's capsule.Cell fractionation: Cell fractionation is the separation of homogeneous sets from a larger population of cells.Intermediate filament: Intermediate filaments (IFs) are cytoskeletal components found in the cells of many animal species.Karabinos, Anton, Dieter Riemer, Andreas Erber, and Klaus Weber.Stratified squamous epithelium: A stratified squamous epithelium consists of squamous (flattened) epithelial cells arranged in layers upon a basal membrane. Only one layer is in contact with the basement membrane; the other layers adhere to one another to maintain structural integrity.Primary and secondary antibodies: Primary and secondary antibodies are two groups of antibodies that are classified based on whether they bind to antigens or proteins directly or target another (primary) antibody that, in turn, is bound to an antigen or protein.Microtome: A microtome (from the Greek mikros, meaning "small", and temnein, meaning "to cut") is a tool used to cut extremely thin slices of material, known as sections. Important in science, microtomes are used in microscopy, allowing for the preparation of samples for observation under transmitted light or electron radiation.Microtubule: Microtubules ([+ tube] + [are a component of the [[cytoskeleton], found throughout the [[cytoplasm. These tubular polymers of tubulin can grow as long as 50 micrometres and are highly dynamic.Quentin Turnbull: Quentin Turnbull is a fictional character featured in DC Comics' Jonah Hex series. He first appeared in Weird Western Tales #22 (March–April 1974).Symmetry element: A symmetry element is a point of reference about which symmetry operations can take place. In particular, symmetry elements can be centers of inversion, axes of rotation and mirror planes.Mediated transportCryofixation: Cryofixation is a technique for fixation or stabilisation of biological materials as the first step in specimen preparation for electron microscopy. Typical specimens for cryofixation include small samples of plant or animal tissue, cell suspensions of microorganisms or cultured cells, suspensions of viruses or virus capsids and samples of purified macromolecules, especially proteins.Tonofibril: Tonofibrils, discovered by Rudolf Heidenhain, and first described in detail by Louis-Antoine Ranvier, are cytoplasmic protein structures in epithelial tissues that converge at desmosomes and hemidesmosomes. Characterized by the fine fibrils in the epithelial cells.Endoplasmic Reticulum Stress in Beta Cells: Beta cells are heavily engaged in the synthesis and secretion of insulin. They are therefore particularly sensitive to endoplasmic reticulum (ER) stress and the subsequent unfolded protein response(UPR).Endocytosis: Endocytosis is a form of active transport in which a cell transports molecules (such as proteins) into the cell ([+ cytosis]) by engulfing them in an [[energy-using process. Endocytosis and its counterpart, exocytosis, are used by all cells because most chemical substances important to them are large polar molecules that cannot pass through the hydrophobic plasma or cell membrane by passive means.Ganoine: Ganoine or ganoin is a glassy, often multi-layered mineralized tissue that covers the scales, cranial bones and fin rays in some basal ray-finned fishes. It is composed of rod-like, pseudoprismatic apatite crystallites, with less than 5% of organic matter.Actin remodeling of neurons: Actin remodeling is a biochemical process in cells. In the actin remodeling of neurons, the protein actin is part of the process to change the shape and structure of dendritic spines.Margaret Jope: Margaret Jope (1913–2004) was a Scottish biochemist, born as Henrietta Margaret Halliday in Peterhead, Scotland.Proximity ligation assay: Proximity ligation assay (in situ PLA) is a technology that extends the capabilities of traditional immunoassays to include direct detection of proteins, protein interactions and modifications with high specificity and sensitivity. Protein targets can be readily detected and localized with single molecule resolution and objectively quantified in unmodified cells and tissues.Synapto-pHluorin: Synapto-pHluorin is a genetically encoded optical indicator of vesicle release and recycling. It is used in neuroscience to study transmitter release.Fluorescent tag: In molecular biology and biotechnology, a fluorescent tag, also known as a label or probe, is a molecule that is attached chemically to aid in the labeling and detection of a biomolecule such as a protein, antibody, or amino acid. Generally, fluorescent tagging, or labeling, uses a reactive derivative of a fluorescent molecule known as a fluorophore.Podocin: Podocin is a protein component of the filtration slits of podocytes. Glomerular capillary endothelial cells, the glomerular basement membrane and the filtration slits function as the filtration barrier of the kidney glomerulus.Raji cell: Raji cell line is the first continuous human cell line from hematopoietic origin. The cell lines produce an unusual strain of Epstein-Barr virus which will both transform cord blood lymphocytes and induce early antigens in Raji cells.Protein turnover: Protein turnover is the balance between protein synthesis and protein degradation. More synthesis than breakdown indicates an anabolic state that builds lean tissues, more breakdown than synthesis indicates a catabolic state that burns lean tissues.Ligation-independent cloning: Ligation-independent cloning (LIC) is a form of molecular cloning that is able to be performed without the use of restriction endonucleases or DNA ligase. This allows genes that have restriction sites to be cloned without worry of chopping up the insert.MicrovillusBeef cattle: Beef cattle are cattle raised for meat production (as distinguished from dairy cattle, used for milk production). The meat of adult cattle is known as beef.Ferric uptake regulator family: In molecular biology, the ferric uptake regulator (FUR) family of proteins includes metal ion uptake regulator proteins. These are responsible for controlling the intracellular concentration of iron in many bacteria.Chicken as biological research model: Chickens (Gallus gallus domesticus) and their eggs have been used extensively as research models throughout the history of biology. Today they continue to serve as an important model for normal human biology as well as pathological disease processes.Lysosome: A lysosome (derived from the Greek words lysis, meaning "to loosen", and soma, "body") is a membrane-bound cell organelle found in most animal cells (they are absent in red blood cells). Structurally and chemically, they are spherical vesicles containing hydrolytic enzymes capable of breaking down virtually all kinds of biomolecules, including proteins, nucleic acids, carbohydrates, lipids, and cellular debris.Atomic force acoustic microscopy: Atomic Force Acoustic Microscopy (AFAM) is a type of scanning probe microscopy (SPM). It is a combination of acoustics and atomic force microscopy.Actinin: Actinin is a microfilament protein. α-Actinin is necessary for the attachment of actin filaments to the Z-lines in skeletal muscle cells, and to the dense bodies in smooth muscle cells.Endosome: In biology, an endosome is a membrane-bounded compartment inside eukaryotic cells. It is a compartment of the endocytic membrane transport pathway from the plasma membrane to the lysosome.
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