Fluorescent tag: In molecular biology and biotechnology, a fluorescent tag, also known as a label or probe, is a molecule that is attached chemically to aid in the labeling and detection of a biomolecule such as a protein, antibody, or amino acid. Generally, fluorescent tagging, or labeling, uses a reactive derivative of a fluorescent molecule known as a fluorophore.Heptamethine dyesRhodamineFluorescein diacetate hydrolysis: Fluorescein diacetate (FDA) hydrolysis assays can be used to measure enzyme activity produced by microbes in a sample. A bright yellow glow is produced and is strongest when enzymatic activity is greatest.PyridiniumTotal internal reflection fluorescence microscope: A total internal reflection fluorescence microscope (TIRFM) is a type of microscope with which a thin region of a specimen, usually less than 200 nm can be observed.Vital stain: A vital stain in a casual usage may mean a stain that can be applied on living cells without killing them. Vital stains have been useful for diagnostic and surgical techniques in a variety of medical specialties.PrinomastatAcid dye: An acid dye is a dye which is a salt of a sulfuric, carboxylic or phenolic organic acid. The salts are often sodium or ammonium salts.Hair dye stripping: Hair dye stripping is a process used to rid the hair of unwanted deposited color.Spectrofluorometer: A spectrofluorometer is an instrument which takes advantage of fluorescent properties of some compounds in order to provide information regarding their concentration and chemical environment in a sample. A certain excitation wavelength is selected, and the emission is observed either at a single wavelength, or a scan is performed to record the intensity versus wavelength, also called an emission spectra.AutofluorescenceAlkaliphile: Alkaliphiles are a class of extremophilic microbes capable of survival in alkaline (pH roughly 8.5-11) environments, growing optimally around a pH of 10.FuchsineCalcium signaling: Calcium ions are important for cellular signalling, as once they enter the cytosol of the cytoplasm they exert allosteric regulatory effects on many enzymes and proteins. Calcium can act in signal transduction resulting from activation of ion channels or as a second messenger caused by indirect signal transduction pathways such as G protein-coupled receptors.Mauna Kea Technologies: Mauna Kea Technologies is a global medical device company focused on leading innovation in endomicroscopy, the field of microscopic imaging during endoscopy procedures. The company researches, develops and markets tools to visualize, detect and rule out abnormalities including malignant and pre-malignant tumors or lesions in the gastrointestinal and pulmonary tracts.SSR-180,575FluoresceinLynch Syndrome InternationalCarboxyfluorescein diacetate succinimidyl esterTetramethylammonium chlorideFura-2Gap junction: A gap junction may also be called a nexus or macula communicans. When found in nerves they are also referred to as an electrical synapse.Reversal potential: In a biological membrane, the reversal potential (also known as the Nernst potential) of an ion is the membrane potential at which there is no net (overall) flow of that particular ion from one side of the membrane to the other. In the case of post-synaptic neurons, the reversal potential is the membrane potential at which a given neurotransmitter causes no net current flow of ions through that neurotransmitter receptor's ion channel.Flow cytometry: In biotechnology, flow cytometry is a laser-based, biophysical technology employed in cell counting, cell sorting, biomarker detection and protein engineering, by suspending cells in a stream of fluid and passing them by an electronic detection apparatus. It allows simultaneous multiparametric analysis of the physical and chemical characteristics of up to thousands of particles per second.Fluorescence loss in photobleaching: Fluorescence Loss in Photobleaching (FLIP) is a fluorescence microscopy technique used to examine movement of molecules inside cells and membranes. A cell membrane is typically labeled with a fluorescent dye to allow for observation.Ethidium homodimer assay: An ethidium homodimer assay can be used to detect dead or dying cells. Ethidium homodimer is a membrane-impermeable fluorescent dye which binds to DNA.ClemizoleBurst kinetics: Burst kinetics is a form of enzyme kinetics that refers to an initial high velocity of enzymatic turnover when adding enzyme to substrate. This initial period of high velocity product formation is referred to as the "Burst Phase".Gelsemium: Gelsemium is an Asian and North American genus of flowering plants belonging to family Gelsemiaceae. The genus contains three species of shrubs to straggling or twining climbers.BenzothiazoleTemporal analysis of products: Temporal Analysis of Products (TAP), (TAP-2), (TAP-3) is an experimental technique for studyingNile redLiposomeLaser-induced breakdown spectroscopy: Laser-induced breakdown spectroscopy (LIBS) is a type of atomic emission spectroscopy which uses a highly energetic laser pulse as the excitation source. The laser is focused to form a plasma, which atomizes and excites samples.Multiphoton fluorescence microscope: A multiphoton fluorescence microscope (MFM) is a specialized optical microscope.Naphthol Green BCell membraneCapillary electrophoresis–mass spectrometry: Capillary electrophoresis–mass spectrometry (CE-MS) is an analytical chemistry technique formed by the combination of the liquid separation process of capillary electrophoresis with mass spectrometry. CE-MS combines advantages of both CE and MS to provide high separation efficiency and molecular mass information in a single analysis.Near-infrared spectroscopyFilter fluorometer: A filter fluorometer is a type of fluorometer that may be employed in fluorescence spectroscopy.Connexon: In biology, a connexon, also known as a connexin hemichannel or a pannexin channel, is an assembly of six proteins called connexins that form the pore for a gap junction between the cytoplasm of two adjacent cells. This channel allows for bidirectional flow of ions and signaling molecules.Biotinylated dextran amine: Biotinylated dextran amines (BDA) are organic compounds used as anterograde and retrograde neuroanatomical tracers. They can be used for labeling the source as well as the point of termination of neural connections and therefore to study neural pathways.Mediated transportZinc ammonium chloride: Zinc ammonium chloride, also known as diammonium tetrachlorozincate(2-) (IUPAC name), is a zinc salt commonly used as a flux in the process of hot-dip galvanizing. The CAS registry number is 14639-97-5.Gene gunSingle-molecule FRET: Single molecule fluorescence resonance energy transfer (or smFRET) is a biophysical technique used to measure distances at the 1-10 nanometer scale in single molecules, typically biomolecules. It is an application of FRET wherein a single donor and acceptor FRET pairs are excited and detected.Fractional sodium excretion: The fractional excretion of sodium (FENa) is the percentage of the sodium filtered by the kidney which is excreted in the urine. It is measured in terms of plasma and urine sodium, rather than by the interpretation of urinary sodium concentration alone, as urinary sodium concentrations can vary with water reabsorption.DNA condensation: DNA condensation refers to the process of compacting DNA molecules in vitro or in vivo. Mechanistic details of DNA packing are essential for its functioning in the process of gene regulation in living systems.MICAD: == Molecular Imaging and Contrast Agent Database ==Sodium–hydrogen antiporter: The sodium–hydrogen antiporter or sodium–hydrogen exchanger is a membrane protein found in many cells, and especially in those of the nephron of the kidney. Specifically in the intercalary cells of the collecting duct.Propidium iodidePeriodic current reversalBarbiturate dependenceOrganotin chemistryTrans-tubular potassium gradient: The trans-tubular potassium gradient (TTKG) is an index reflecting the conservation of potassium in the cortical collecting ducts (CCD) of the kidneys. It is useful in diagnosing the causes of hyperkalemia or hypokalemia.HyperchloremiaSalting in: Salting in refers to the effect where increasing the ionic strength of a solution increases the solubility of some solute (such as a protein). This effect tends to be observed at lower ionic strengths.New Zealand rabbitHSD2 neurons: HSD2 neurons are a small group of neurons in the brainstem which are uniquely sensitive to the mineralocorticosteroid hormone aldosterone, through expression of HSD11B2. They are located within the caudal medulla oblongata, in the nucleus of the solitary tract (NTS).BenzofuranIndoleMass diffusivity: Diffusivity or diffusion coefficient is a proportionality constant between the molar flux due to molecular diffusion and the gradient in the concentration of the species (or the driving force for diffusion). Diffusivity is encountered in Fick's law and numerous other equations of physical chemistry.Cytometry: Cytometry is the measurement of the characteristics of cells. Variables that can be measured by cytometric methods include cell size, cell count, cell morphology (shape and structure), cell cycle phase, DNA content, and the existence or absence of specific proteins on the cell surface or in the cytoplasm.Nanoparticle: Nanoparticles are particles between 1 and 100 nanometers in size. In nanotechnology, a particle is defined as a small object that behaves as a whole unit with respect to its transport and properties.Avo PhotonicsBoron carbideInhibitor protein: The inhibitor protein (IP) is situated in the mitochondrial matrix and protects the cell against rapid ATP hydrolysis during momentary ischaemia. In oxygen absence, the pH of the matrix drops.
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