The assay started to be used in biological research in the early 1980s. Intracellular bacteria need to enter host cells (cells ... HeLa cells are commonly used as eukaryotic cells in the gentamicin protection assay, but other cells can be used as well. As ... The gentamicin protection assay or survival assay or invasion assay is a method used in microbiology. It is used to quantify ... should be included in the assay. An alternative invasion assay is the differential immunostaining assay, based on the binding ...
Here she developed nanoparticle sensors for biological assays. In particular, Thanh used gold nanoparticles combined with ... She developed a technique that could be used to synthesise cell membrane permeable fluorescent versions of cyclic adenosine ...
Synthesis by mast cell-fibroblast monolayers during lymphocyte-dependent mast cell proliferation". The Journal of Biological ... This property is exploited in a range of molecular biological assays. Common diagnostic procedures require PCR amplification of ... Current clinical laboratory assays for heparin rely on an indirect measurement of the effect of the drug, rather than on a ... Heparin is produced by basophils and mast cells in all mammals. The discovery of heparin was announced in 1916. It is on the ...
This delivers label-free, non-activated cells suitable for immunologic or cell biological assays. The technology can be used ... T cells, B cells and other cells of interest. This technology is known as Fab-TACS (Traceless Affinity Cell Selection) and is ... Isolated and purified cells are being deployed for basic research as well as diagnostic applications. The Streptamers for cell ... Recently, the method was further developed to be used for cell selection from whole blood or other single cell suspensions. IBA ...
... biosensing for various measurements such as enzyme kinetics and biological assays of many other types of cells. Increased ... Hydrocarbon oil is typically not used in cell microfluidic research because it is not compatible with cells and damages cell ... Kintses B, Hein C, Mohamed MF, Fischlechner M, Courtois F, Lainé C, Hollfelder F (August 2012). "Picoliter cell lysate assays ... Droplets are also capable of being transformed into cell-like structures which can be used to mimic humans' biological ...
Biological immortality Cell culture assays Microphysiometry Organ culture Plant tissue culture Tissue culture Jayme DW, Smith ... A chemically defined medium is a growth medium suitable for the in vitro cell culture of human or animal cells in which all of ... list of Serum-Free media Impact of surfactants in cell culture media on HEK and CHO cell cultivation and transfection Fetal ... Standard cell culture media commonly consist of a basal medium supplemented with animal serum (such as fetal bovine serum, FBS ...
The activity of the multidrug transporter in drug-resistant cells is associated with rapid cellular ATP depletion when ATP ... The Journal of Biological Chemistry. 267 (7): 4854-8. PMID 1347044. (Articles with short description, Short description matches ... The ATPase assay is a membrane assay that indirectly measures the activity of efflux transporters. ATP Binding Cassette or ... The ATPase assay is designed to indicate the nature of the interaction between the compound and the transporter. The ATPase ...
The discovery of eotaxin is also linked to a biological assay that was conducted on guinea pigs. This biological assay was ... Some examples of the types of cells that have the ability of synthesizing eotaxin are lung cells, vascular endothelial cells, ... When eotaxin binds with the CCR 3 Receptor, it induces biological changes. Some examples of these biological changes are the ... These eosinophils are strong, pro-inflammatory effector cells. The cells involved in allergic responses, such as eosinophils, ...
Using liquid chromatography in conjunction with several biological assays, PDF, was also isolated in the insect Leucophaea ... Type 1 cells showed that PDF signaled via elevation of intracellular cAMP levels. In contrast, in type 2 cells PDF transiently ... The movement and aggregation of pigments in retina cells and extra-retinal cells is hypothesized to be under a split hormonal ... Their study showed that M-cells periodically send a "reset" signal which determines the oscillations of the E-cells. It is ...
CETSA® can also be conducted on cell lysates versus intact cells, helping to determine sample penetration of the cell membrane ... "A thermal stability assay can help to estimate the crystallization likelihood of biological samples". Acta Crystallographica. ... July 2013). "Monitoring drug target engagement in cells and tissues using the cellular thermal shift assay". Science. 341 (6141 ... Biochemical assays require a catalytic activity of the protein in question as well as a specific assay. Circular dichroism and ...
MTT assay Cell Counting Kit-8 (WST-8 based cell viability assay) SRB (Sulforhodamine B) assay CellTiter-Glo® Luminescent Cell ... biochemical assays) may be similar to chemical analysis and titration. However, assays typically involve biological material or ... BCA assay) Bradford protein assay Lowry protein assay Secretion assay Nuclear run-on Ribosome profiling A cell-counting assay ... Cell fractionation or Immunoprecipitation Migration assays, Chemotaxis assay Secretion assays Apoptosis assays such as the DNA ...
Prior to his role as CEO at Biological Dynamics, Dr. Billings was the Chief Medical Officer at Natera, a leader in cell-free ... Biological Dynamics' ExoVita™ assays utilize the ExoVerita platform to enable reliable surveillance and early cancer detection ... Clinical Validation of a Plasma Donor-derived Cell-free DNA Assay to Detect Allograft Rejection and Injury in Lung Transplant. ... A novel donor-derived cell-free DNA assay for the detection of acute rejection in heart transplantation. J Heart Lung ...
Zebrafish AB9 Biological immortality Cell culture assays Electric cell-substrate impedance sensing List of contaminated cell ... cells Cell-to-cell contact can stimulate cell cycle arrest, causing cells to stop dividing, known as contact inhibition. Cell- ... These cells may be cells isolated from a donor organism (primary cells) or an immortalised cell line. The cells are bathed in a ... Plant cell lines Tobacco BY-2 cells (kept as cell suspension culture, they are model system of plant cell) Other species cell ...
To ensure that the interaction with the intended target structure is measured appropriate biological controls, such as cells ... "Assay Acceptance Criteria for Multiwell-Plate-Based Biological Potency Assays". BioProcess International. 12 (1): 30-41. "SIMAG ... In this type of assay the binding of a ligand to cells is followed over time. The obtained signal is proportional to the number ... In a filter binding assay, the filters are used to trap cell membranes by sucking the medium through them. This rapid method ...
Herblot, Sabine; Aplan, Peter D.; Hoang, Trang (2002-02-01). "Gradient of E2A Activity in B-Cell Development". Molecular and ... The Journal of Biological Chemistry. 273 (14): 8278-86. doi:10.1074/jbc.273.14.8278. PMID 9525934. Langlands K, Yin X, Anand G ... "Analysis of the oligomerization of myogenin and E2A products in vivo using a two-hybrid assay system". The Journal of ... Deed RW, Jasiok M, Norton JD (Apr 1998). "Lymphoid-specific expression of the Id3 gene in hematopoietic cells. Selective ...
In cell culture, myogenin can induce myogenesis in a variety of non-muscle cell types. Myogenin has been shown to interact with ... The Journal of Biological Chemistry. 271 (9): 5258-64. doi:10.1074/jbc.271.9.5258. PMID 8617811. Langlands K, Yin X, Anand G, ... "Analysis of the oligomerization of myogenin and E2A products in vivo using a two-hybrid assay system". The Journal of ... Myogenin is required for the proper differentiation of most myogenic precursor cells during the process of myogenesis. When the ...
Determined by consideration of all biological assay methods (c. 1970): The ovaries and adrenal glands also produce androgens, ... These are Leydig cells. Soon after they differentiate, Leydig cells begin to produce androgens. The androgens function as ... Determined by consideration of all biological assay methods (c. 1970): 5α-Dihydrotestosterone (DHT) was 2.4 times more potent ... The mesoderm-derived epithelial cells of the sex cords in developing testes become the Sertoli cells, which will function to ...
Since this conjugate may be rapidly exported from the cell and has not yet been characterized for biological activity, it is ... "Lipid G Protein-coupled Receptor Ligand Identification Using β-Arrestin Path Hunter Assay". Journal of Biological Chemistry. ... Evidence for a role of the reticulocyte lipoxygenase in the maturation of red cells". The Journal of Biological Chemistry. 265 ... Statins also inhibit PPARγ in human macrophages, vascular endothelial cells, and smooth muscle cells; this action may ...
Multicolor optical coding for biological assays has been achieved by embedding different-sized quantum dots into polymeric ... It is difficult to track a small group of cells throughout the body, so scientists used to dye the cells. These dyes needed to ... Complex drug delivery mechanisms are being developed, including the ability to get drugs through cell membranes and into cell ... K. Eric Drexler, one of the founders of nanotechnology, postulated cell repair machines, including ones operating within cells ...
Subsequent analysis using a commercial assay also indicated that ENOblock can inhibit enolase activity in biological contexts, ... It is expressed at much lower levels in most mammalian cells. When present in the same cell, different isozymes readily form ... It is present at some level in all normal human cells. ββ or muscle-specific enolase (MSE). Also known as enolase 3. This ... Three of these isoenzymes (all homodimers) are more commonly found in adult human cells than the others: αα or non-neuronal ...
An assay is a type of biological titration used to determine the concentration of a virus or bacterium. Serial dilutions are ... The positive or negative value may be determined by inspecting the infected cells visually under a microscope or by an ... Gay-Lussac (1824). "Instruction sur l'essai du chlorure de chaux" [Instructions on the assaying of chlorinated lime]. Annales ... New experiments on the assay of commercial potash and an apparatus called a "potassimeter" to perform it]. Journale de ...
Mycoplasma bacterial infections greatly affects the cell mediator release for biological assays, therefore regular Mycoplasma ... RBL (Rat Basophilic Leukemia cells) are cancerous basophil cells that are well suited for the study of allergies. RBL cells are ... They are used in place of mast cells due to a higher stability in tissue culture. RBL cells can also be humanized, aiding in ... Rodent cell lines, All stub articles, Cell biology stubs). ... "Use of Humanized Rat Basophilic Leukemia Reporter Cell Lines as ...
... in cell-based systems uses living cells as tools in biological research to elucidate the workings of normal and diseased cells ... in which thousands of compounds are tested in parallel for their activity in one or more biological assays, but involves assays ... Given the increase in the use of phenotypic/visual screening as a cell biological tool, methods are required that permit ... Changes in cell morphology are then detected using image analysis. Changes in the amounts of proteins synthesized by cells are ...
The biological activity of fisetin has been studied in many laboratory assays; like other polyphenols it has many activities. ... Studies of cell cultures of senescent human umbilical vein endothelial cells have shown that fisetin induces apoptosis by ... Like the other compounds, it has also been shown to be reactive in many different assays of biological activities, raising the ... Studies suggest that it lodges in cell membranes and prevents oxidative damage to lipids in the cell membrane. Like other ...
The neurosphere assay, or stemness assay, has been used to confirm that neurospheres contain neural stem cells. Neurospheres ... However useful the neurosphere culture has been for biological studies of developmental processes and the functional assay for ... After two days in vitro, most of the cells had died, but 15±2 cells for each plate were undergoing cell division. This ... Cerebral organoid Neural stem cell Progenitor cell Adult stem cell Kempermann, Gerd. Adult Neurogenesis. Oxford University ...
Wienken CJ, Baaske P, Rothbauer U, Braun D, Duhr S (2010). "Protein-binding assays in biological liquids using microscale ... Small molecules can have a variety of biological functions or applications, serving as cell signaling molecules, drugs in ... Small molecules may be used as research tools to probe biological function as well as leads in the development of new ... Pharmacology usually restricts the term "small molecule" to molecules that bind specific biological macromolecules and act as ...
... the Salk Institute for Biological Studies". ASSAY and Drug Development Technologies. 1 (6): 749-754. doi:10.1089/ ... Harpold, M. M.; Evans, R. M.; Salditt-Georgieff, M.; Darnell, J. E. (1979). "Production of mRNA in Chinese hamster cells: ... Glaser, Vicki (December 2003). "An Interview with Ronald M. Evans, Ph.D. Investigator, The Salk Institute for Biological ... "Salk scientist awarded Glenn T. Seaborg Medal". Salk Institute for Biological Studies. Retrieved 2019-10-05. Foundation, Lasker ...
Molecular Cell Biology. 4 (5): 397-407. doi:10.1038/nrm1103. PMID 12728273. S2CID 22622224. Ye C, Zhang Z, Wang Z, Hua Q, Zhang ... Using β-arrestin2 recruitment and cAMP accumulation assays, it was recently found that cannabidiol is an inverse agonist for ... The Journal of Biological Chemistry. 284 (18): 12328-12338. doi:10.1074/jbc.M806516200. PMC 2673301. PMID 19286662. Godlewski G ... doi:10.1016/j.cell.2021.04.037. PMC 8238500. PMID 34048700. Schultz RM, Montgomery RR, Belanoff JR (June 1983). "Regulation of ...
It is a two-dimensional array on a solid substrate-usually a glass slide or silicon thin-film cell-that assays (tests) large ... The fabrication of the microarray requires both biological and physical information, including sample libraries, printers, and ... These assays allow high-throughput analysis in a controlled environment without inferences associated with intact cells. ... which makes the cloning and expression of proteins in host cells obsolete, because no intact cells are needed. The molecule of ...
Wienken CJ, Baaske P, Rothbauer U, Braun D, Duhr S (October 2010). "Protein-binding assays in biological liquids using ... "Heterobivalent ligands target cell-surface receptor combinations in vivo". Proceedings of the National Academy of Sciences of ... Ligand efficacy refers to the ability of the ligand to produce a biological response upon binding to the target receptor and ... In biochemistry and pharmacology, a ligand is a substance that forms a complex with a biomolecule to serve a biological purpose ...
Together with biological assay, these methods greatly facilitate the DNA methylation analysis. 5-Hydroxymethylcytosine 5- ... such as the inner cell mass of the blastocyst, primordial germ cells or embryonic stem cells. Since DNA methylation appears to ... DNA methylation marks - genomic regions with specific methylation patterns in a specific biological state such as tissue, cell ... If the mismatch is not repaired and the cell enters the cell cycle the strand carrying the T will be complemented by an A in ...