Polynucleotide 5'-hydroxyl-kinase
... hydroxyl kinase, DNA kinase, polynucleotide kinase, and polynucleotide 5'-hydroxy-kinase. Novogrodsky A, Hurwitz J (1966). "The ... hydroxyl polynucleotide kinase". J. Biol. Chem. 241 (12): 2933-43. PMID 4287930. Vivo New England Biolabs' T4 PNK page Portal: ... hydroxyl polynucleotide kinase, 5'-hydroxyl polyribonucleotide kinase, 5'-hydroxyl RNA kinase, DNA 5'- ... Polynucleotide kinase is a T7 bacteriophage (or T4 bacteriophage) enzyme that catalyzes the transfer of a gamma-phosphate from ...
PNK
... hydroxyl-kinase, an enzyme Pauna language (ISO 639-3 language code: pnk) Pinnacle Entertainment (Stock symbol: PNK) Supadio ... PNK or pnk may refer to: Polynucleotide 5'- ...
List of MeSH codes (D08)
... hydroxyl-kinase MeSH D08.811.913.696.620.682 - protein kinases MeSH D08.811.913.696.620.682.650 - phosphorylase kinase MeSH ... polynucleotide 5'-hydroxyl-kinase MeSH D08.811.150.240 - dna modification methylases MeSH D08.811.150.280 - dna restriction ... map kinase kinase kinase 1 MeSH D08.811.913.696.620.682.700.559.200 - map kinase kinase kinase 2 MeSH D08.811.913.696.620.682. ... map kinase kinase kinase 3 MeSH D08.811.913.696.620.682.700.559.400 - map kinase kinase kinase 4 MeSH D08.811.913.696.620.682. ...
Base excision repair
In humans, polynucleotide kinase-phosphatase (PNKP) promotes formation of these ends during BER. This protein has a kinase ... In order for ligation to occur, a DNA strand break must have a hydroxyl on its 3' end and a phosphate on its 5' end. ... 3'-Processing must occur before DNA synthesis can initiate because DNA polymerases require a 3' hydroxyl to extend from. Pol β ... The AP endonucleases cleave an AP site to yield a 3' hydroxyl adjacent to a 5' deoxyribosephosphate (dRP). AP endonucleases are ...
List of EC numbers (EC 2)
... deoxyadenosine kinase EC 2.7.1.77: nucleoside phosphotransferase EC 2.7.1.78: polynucleotide 5′-hydroxyl-kinase EC 2.7.1.79: ... subunit kinase EC 2.7.11.24: mitogen-activated protein kinase EC 2.7.11.25: mitogen-activated protein kinase kinase kinase EC ... NAD+ kinase EC 2.7.1.24: dephospho-CoA kinase EC 2.7.1.25: adenylyl-sulfate kinase EC 2.7.1.26: riboflavin kinase EC 2.7.1.27: ... acetate kinase EC 2.7.2.2: carbamate kinase EC 2.7.2.3: phosphoglycerate kinase EC 2.7.2.4: aspartate kinase EC 2.7.2.5: Now EC ...
Forkhead-associated domain
The forkhead associated (FHA) domain of polynucleotide kinase phosphatase (PNKP) is necessary for the recruitment of PNKP to ... hydroxyl ends, and the phosphatase domain removes phosphates from 3' ends. These activities, acting together, prepare single- ... In these activities PNKP acts both as a kinase and a phosphatase in the ligation of DNA ends. The kinase domain phosphorylates ... Hofmann K, Bucher P (September 1995). "The FHA domain: a putative nuclear signalling domain found in protein kinases and ...
Hybridization assay
... ends of the analyte by the joint action of T4 DNA ligase and T4 polynucleotide kinase. The kinase phosphorylates the 5'-end of ... One limitation of the method is that it requires a free 3'-end hydroxyl which may not be available when targeting moieties are ... end requiring to have a free hydroxyl (or a phosphate group). Further, T4 DNA ligases are incompatible with ligation of RNA ... The limitations with the hybridization-ligation assay also apply to the dual ligation assay, with the 5'-end in addition to the ...
Ribonucleotide
Instead of a hydroxyl group at the second carbon in the ribose ring, it is replaced by a hydrogen atom. Both types of pentoses ... There is no phosphodiester bond at each end of the polynucleotide. Phosphodiester bonds are formed between ribonucleotides by ... They are first reduced by RNR and then phosphorylated by nucleoside diphosphate kinases to dATP and dGTP. Ribonucleotide ... hydroxyl group of the last ribonucleotide in the chain acts as a nucleophile and launches a hydrophilic attack on the 5'- ...
TRNA-intron endonuclease
It was found that the tRNA splicing endonuclease in humans reacts with a polynucleotide kinase known as CLP1. The exact ... hydroxyl terminus. The use of tRNA-intron endonuclease in pre-tRNA intron excision is just one of the steps for tRNA maturation ... hydroxyl terminus This enzyme catalyses one of the beginning stages in the maturation of tRNA molecules. Organisms from every ... At the 5' end, cleavage occurs typically at the canonical position which is one nucleotide 3′ to the anticodon. At the 3' end, ...
Hepatitis delta virus ribozyme
... cyclic phosphate that can easily be removed by treatment with a phosphatase or T4 polynucleotide kinase. The target RNA can ... hydroxyl. In addition, it is possible that the phosphate of U23 can act as a Lewis acid to accept a proton from the 2′-hydroxyl ... A magnesium ion in the active site of the ribozyme is coordinated to the 2'-hydroxyl nucleophile and an oxygen of the scissile ... hydroxyl leaving group, it is also now accepted that the HDV ribozyme can use a metal ion to assist in activation of the 2′- ...
Thymidine kinase
The analog has been modified so that it does not have the hydroxyl group in the 3'-position that is required for continued ... Reichard P, Estborn B (February 1951). "Utilization of desoxyribosides in the synthesis of polynucleotides". The Journal of ... Thymidine kinase 1 Thymidine kinase from herpesvirus Thymidylate kinase Nucleoside-diphosphate kinase Thymidylate synthase ... Thymidine kinase is an enzyme, a phosphotransferase (a kinase): 2'-deoxythymidine kinase, ATP-thymidine 5'-phosphotransferase, ...
Ligation (molecular biology)
... therefore it needs to be phosphorylated by treatment with T4 polynucleotide kinase. Blunt-end ligation is also reversibly ... Finally, a phosphodiester bond between the two DNA ends is formed via the nucleophilic attack of the 3'-hydroxyl at the end of ... Raae AJ, Kleppe RK, Kleppe K (December 1975). "Kinetics and effect of salts and polyamines on T4 polynucleotide ligase". ... hydroxyl group (-OH) at one end of a strand of DNA and the 5'-phosphate group (-PO4) of another. In animals and bacteriophages ...
Biology
In pure water, the number of hydrogen ions balances (or equals) the number of hydroxyl ions, resulting in a pH that is neutral ... DNA is composed of two polynucleotide chains that coil around each other to form a double helix. It is found as linear ... Other types of receptors include protein kinase receptors (e.g., receptor for the hormone insulin) and G protein-coupled ... As a molecule, water is not completely stable as each water molecule continuously dissociates into hydrogen and hydroxyl ions ...
Glossary of genetics (M-Z)
The opposite process is performed by kinases. phosphate backbone The linear chain of alternating phosphate and sugar compounds ... saturation hybridization An in vitro nucleic acid hybridization reaction in which one polynucleotide component (either DNA or ... hydroxyl group from the ribose ring of ribonucleoside diphosphates (rNDPs). RNR plays a critical role in regulating the overall ... where ribose has an attached hydroxyl group that deoxyribose lacks. ribosomal DNA (rDNA) ribosomal RNA (rRNA) ribosome A ...
Protein biosynthesis
The phosphate group is removed from the donor molecule ATP by a protein kinase and transferred onto the hydroxyl group of the ... DNA has an antiparallel, double helix structure composed of two, complementary polynucleotide strands, held together by ... the phosphodiester bonds connecting the nucleotides are formed by joining the hydroxyl group on the 3' carbon of one nucleotide ... The ribosome reads the mRNA molecule in a 5'-3' direction and uses it as a template to determine the order of amino acids in ...
Nucleotide
UMP is phosphorylated by two kinases to uridine triphosphate (UTP) via two sequential reactions with ATP. First, the ... Abbreviations and Symbols for Nucleic Acids, Polynucleotides and their Constituents (IUPAC) Provisional Recommendations 2004 ( ... hydroxyl groups of the sugar. Some signaling nucleotides differ from the standard single-phosphate group configuration, in ... 5-nucleotides are also used in flavour enhancers as food additive to enhance the umami taste, often in the form of a yeast ...
List of EC numbers (EC 3)
... polynucleotide 3′-phosphatase EC 3.1.3.33: polynucleotide 5′-phosphatase EC 3.1.3.34: deoxynucleotide 3′-phosphatase EC 3.1. ... pyruvate kinase]-phosphatase EC 3.1.3.50: sorbitol-6-phosphatase EC 3.1.3.51: dolichyl-phosphatase EC 3.1.3.52: [3-methyl-2- ... N ω-hydroxy-L-arginine amidinohydrolase * EC 3.5.3.26: (S)-ureidoglycine aminohydrolase * *No Wikipedia article EC 3.5.4.1: ... EC 3.8.1.2: (S)-2-haloacid dehalogenase EC 3.8.1.3: haloacetate dehalogenase EC 3.8.1.4: Now EC 1.97.1.10, thyroxine 5′- ...
Glossary of genetics (0-L)
kinase Any of a class of enzymes which catalyze the transfer of phosphate groups from high-energy, phosphate-donating molecules ... excision The enzymatic removal of a polynucleotide sequence from one or more strands of a nucleic acid, or of a polypeptide ... carbon is often still bonded to a hydroxyl group). By convention, sequences and structures positioned nearer to the 3'-end ... 5-methyluracil See thymine. Contents Top A B C D E F G H I J K L M N O P Q R S T U V W X Y Z See also References External links ...