... may refer to: Ligation (molecular biology), the covalent linking of two ends of DNA or RNA molecules Chemical ligation ... a method of attaching the archwires to the brackets KAHA Ligation Ligation-independent cloning Typographic ligature forming ... Look up ligation in Wiktionary, the free dictionary. ... Tubal ligation, a method of female sterilization Rubber band ... ligation, a treatment for hemorrhoids In coordination chemistry, making a bond between a ligand and a Lewis acid In ...

Partial tubal ligation or full salpingectomy (a tubal ligation method that relies upon the physical removal of the fallopian ... Tubal ligation is considered a permanent method of sterilization and birth control. Female sterilization through tubal ligation ... Since most forms of tubal ligation require abdominal surgery under regional or general anesthesia, tubal ligation is also ... "postabortion tubal ligation", or more than six weeks after the end of a pregnancy, termed an "interval tubal ligation". The ...

It is an alternative to the Native Chemical Ligation (NCL). KAHA Ligation was developed by Jeffrey W. Bode group at ETH Zürich ... The first reported protein synthesized by KAHA ligation was human GLP-1 (7-36). Since then, a variety of small proteins (up to ... The most commonly used N-terminal hydroxylamine is the 5-oxaproline, which results in a homoserine residue after ligation and O ... The α-Ketoacid-Hydroxylamine (KAHA) Amide-Forming Ligation is a chemical reaction that is used to join two unprotected ...

The Staudinger ligation continues to be developed but has not yet found widespread use. Ser/Thr ligation was introduced into ... The original chemical ligation methods involved the formation of a non-native bond at the ligation site. Subsequently, native ... Native chemical ligation relies on the presence of a cysteine residue at the ligation site. Methods using removable auxiliary ... Chemical ligation is usually carried out in aqueous solution. Multiple consecutive chemical ligation reactions can be used to ...

... (LIC) is a form of molecular cloning that is able to be performed without the use of restriction ... "Ligation Independent Cloning (LIC)". New England BioLabs (NEB). Retrieved 15 January 2016. "Get Your Clone 90% Of The Time with ... Haun, RS; Serventi, IM; Moss, J (1992). "Rapid, reliable ligation-independent cloning of PCR products using modified plasmid ...

Intein KAHA Ligation Peptide synthesis Protein synthesis SEA Native Peptide Ligation Dawson, P. E.; Muir, T. W.; Clark-Lewis, I ... Alternatively, thiol-containing ligation auxiliaries can be used that mimic an N-terminal cysteine for the ligation reaction, ... Native Chemical Ligation (NCL) is an important extension of the chemical ligation concept for constructing a larger polypeptide ... Native chemical ligation of this kind using a recombinant polypeptide segment is known as Expressed Protein Ligation. Similarly ...

... is a DNA sequencing method that uses the enzyme DNA ligase to identify the nucleotide present at a given ... Sequencing by ligation relies upon the sensitivity of DNA ligase for base-pairing mismatches. The target molecule to be ... Sequencing by ligation can proceed in either direction (either 5'-3' or 3'-5') depending on which end of the probe ... This sequencing by ligation method has been reported to have problems sequencing palindromic sequences. Sequencing by ...

"Haemorrhoidal artery ligation versus rubber band ligation for the management of symptomatic second-degree and third-degree ... The CRH O'Regan ligation system also eliminates the use of forceps. It is much more expensive on a per-case basis than the ... Rubber band ligation is a popular procedure for the treatment of hemorrhoids, as it involves a much lower risk of pain than ... With rubber band ligation, a small band is applied to the base of the hemorrhoid, stopping the blood supply to the hemorrhoidal ...

TA ligation is therefore a form of sticky end ligation. Blunt-ended vectors may be turned into vector for TA ligation with ... Ligation is complicated by the fact that the desired ligation products for most ligation reactions should be between two ... Blunt-end ligation is much less efficient than sticky end ligation, so a higher concentration of ligase is used in blunt-end ... For the ligation reaction to proceed efficiently, the ends should be stably annealed, and in ligation experiments, the Tm of ...

In situ proximity ligation assays (isPLA) has been applied to antibody validation in human tissues with various advantages over ... Proximity ligation assay (in situ PLA) is a technology that extends the capabilities of traditional immunoassays to include ... Roussis IM, Guille M, Myers FA, Scarlett GP (2016). "RNA Whole-Mount In situ Hybridisation Proximity Ligation Assay (rISH-PLA ... Leuchowius KJ, Weibrecht I, Landegren U, Gedda L, Söderberg O (October 2009). "Flow cytometric in situ proximity ligation ...

The overall process of SEA native peptide ligation involves first an N,S-acyl shift for in in situ formation of a peptide ... SEA is an abbreviation of bis(2-sulfanylethyl)amido (Scheme 1). SEA ligation involves the reaction of a peptide featuring a C- ... The first peer reviewed publication describing SEA native peptide ligation was published in Organic Letters by Melnyk, O. et al ... Ollivier, N.; Dheur, J.; Mhidia, R.; Blanpain, A.; Melnyk, O., Bis(2-sulfanylethyl)amino native peptide ligation. Org. Lett. ...

... (MLPA) is a variation of the multiplex polymerase chain reaction that permits ... Multiplex ligation-dependent probe amplification was invented by Jan Schouten, a Dutch scientist. The method was first ... "Multiplex Ligation-dependent Probe Amplification (MLPA)". Bitesize Bio. 2018-12-27. Retrieved 2021-06-07. Yau SC, Bobrow M, ... X and Y based on multiplex ligation-dependent probe amplification (MLPA)". Eur. J. Hum. Genet. 13 (2): 171-175. doi:10.1038/sj. ...

... (DRIL) is a surgical method of treating vascular access steal syndrome. DRIL was ... by arteriovenous fistula with distal artery ligation and revascularization". J Vasc Surg. 7 (6): 770-3. doi:10.1016/0741-5214( ...

... (SICLOPPS) is a biotechnology technique that permits the creation of ...

"Tubal Ligation". Johns Hopkins Medicine. "Vasectomy". Johns Hopkins Medicine. Lin, Luo; Wu Shi-Zhong; Zhu Changmin; Fan Qifu; ... There are multiple ways of having sterilization done, but the two that are used most frequently are tubal ligation for women ... This method is considered more effective than tubal ligation, as there is no chance of tubal reconnection or clip failure, and ... A 1996 Chinese study found that "risk for depression was 2.34 times greater after tubal ligation, and 3.97 times greater after ...

One such method employs the technique of partial ligation. DNA ligation is the process by which linear DNA pieces are connected ... Bowen, R (20 October 1999). "DNA Ligation". Biotechnology and Genetic Engineering. Retrieved 12 November 2013. Lan, Vo Thi ...

1959 Aug;66:640-8. Tubal ligation. Green GH. N Z Med J. 1958 Oct;57(321):470-7. Foetal renal hypoplasia and the origin of ...

In traceless Staudinger ligation, the organophosphorus group dissociates giving a phosphorus-free bioconjugate. Gololobov, Y. G ... In classical Staudinger ligation, the organophosphorus compound becomes incorporated into the peptide. Typically, appended to ... Of interest in chemical biology is the Staudinger ligation, which has been called one of the most important bioconjugation ... Two versions of the Staudinger ligation have been developed. Both begin with the classic iminophosphorane reaction. ...

... the tetrazine ligation, the isocyanide-based click reaction, and most recently, the quadricyclane ligation. The use of ... The Staudinger ligation has been used in both live cells and live mice. The azide can act as a soft electrophile that prefers ... The Staudinger ligation is a reaction developed by the Bertozzi group in 2000 that is based on the classic Staudinger reaction ... The quadricyclane ligation utilizes a highly strained quadricyclane to undergo [2+2+2] cycloaddition with π systems. ...

Glal hydrolysis and Ligation Adapter Dependent PCR assay (GLAD-PCR assay) is the novel method to determine R(5mC)GY sites ... The universal adapter ligation. As an adapter an oligonucleotide duplex 5'-CCTGCTCTTTCATCG-3'/3'-pGGACGAGAAAGTAGCp-5' is used, ...

In native chemical ligation, a peptide C-terminal thioester reacts with a second peptide that has a cysteine residue at its N- ... Peptide and protein ligation. 25 (18): 4953-4965. doi:10.1016/j.bmc.2017.05.020. PMID 28705433. Bunker RD, Mandal K, Bashiri G ... Native chemical ligation of unprotected peptide segments is used to prepare the protein's polypeptide chain, which is then ... The hydrazide is stable to native chemical ligation reaction conditions, and can be converted in situ to a reactive peptide- ...

ISBN 978-4-431-55823-1. Nuijens T, Schmidt M (2019). Enzyme-mediated ligation methods. Humana, New York, NY. ISBN 978-1-4939- ...

... the tetrazine ligation, the isocyanide-based click reaction, and most recently, the quadricyclane ligation. In supramolecular ... A number of chemical ligation strategies have been developed that fulfill the requirements of bioorthogonality, including the 1 ... Blackman, Melissa L.; Royzen, Maksim; Fox, Joseph M. (2008). "The Tetrazine Ligation: Fast Bioconjugation based on Inverse- ... Sletten, Ellen M.; Bertozzi, Carolyn R. (2011). "A Bioorthogonal Quadricyclane Ligation". Journal of the American Chemical ...

Their suturing ligation without excision]". Khirurgiia (in Bulgarian). 43 (4): 65-8. PMID 2097429. Serdev N (1991). "[Free ...

The oxime ligation can then occur readily, and it has been reported that the rate increased up to 400 times under mild acidic ... The Staudinger ligation of azides and phosphine has been used extensively in field of chemical biology. Because it is able to ... Even though Staudinger ligation is a suitable bioconjugation in living cells without major toxicity, the phosphine's ... Lemieux GA, De Graffenried CL, Bertozzi CR (April 2003). "A fluorogenic dye activated by the staudinger ligation". Journal of ...

Endonucleolytic cleavage and ligation "Ptc1". Saccharomyces Genome Database. SGD Project. Retrieved 21 March 2014. Malleshaiah ...

"Multiplex Ligation-Dependent Probe Amplification". The Journal of Molecular Diagnostics. 8 (4): 433-443. doi:10.2353/jmoldx. ... multiplex ligation-dependent probe amplification (MLPA), array comparative genomic hybridization (aCGH), and EHMT1 sequencing. ...

Additional ligation of the intersphincteric fistula tract did not improve the outcome after endorectal advancement flap. ... ligation of intersphincteric fistula tract) procedure. LIFT procedure is based on secure closure of the internal opening and ... the ligation of intersphincteric fistula tract". J Med Assoc Thai. 90 (3): 581-6. PMID 17427539. van Onkelen, RS; Gosselink, MP ... ligation of intersphincteric tract close to the internal opening and removal of intersphincteric tract, scraping out all ...

"Multiplex Ligation-dependent Probe Amplification (MLPA)". bitesizebio.com. 2018-12-27. Retrieved 2022-03-24. "Nablus mask-like ... and multiplex ligation-dependent probe amplification (MLPA) based techniques. Post-natal diagnoses are more common, but ...

He, Ye; Hinklin, Ronald; Chang, Jiyoung; Kiessling, Laura (2004). "Stereoselective N-Glycosylation by Staudinger Ligation". ...