... binding pockets can be exploited for their affinity and thus be labeled. Affinity labels can be broken down into three distinct ... "Affinity labeling-a general method for labeling the active sites of antibody and enzyme molecules". Biochemistry. 1: 1031-9. ... The tag may be either a reporter such as a fluorophore or an affinity label such as biotin or an alkyne or azide for use with ... Affinity labels are a class of enzyme inhibitors that covalently bind to their target causing its inactivation. The hallmark of ...

Vinkenborg JL, Mayer G, Famulok M (September 2012). "Aptamer-based affinity labeling of proteins". Angewandte Chemie. 51 (36): ... Because the affinity of the aptamer also affects its dynamic range and limit of detection, aptamers with a lower affinity may ... Affinity chromatography also depends on the ability of the affinity reagent, such as an aptamer, to bind and release its target ... They exhibit a range of affinities (KD in the pM to μM range), with variable levels of off-target binding and are sometimes ...

Purich DL (2010). "Irreversible Enzyme Inhibition by Affinity Labelling Agents". Enzyme Kinetics: Catalysis and Control: A ... Furthermore, the affinity of the inhibitor for the free enzyme and the enzyme-substrate complex may differ.: 136-139 By ... In these cases some of these inhibitors rapidly bind to the enzyme in a low-affinity EI complex and this then undergoes a ... The Km relating to the affinity of the enzyme for the substrate should in most cases relate to potential changes in the binding ...

See LP label of Affinity release on Discogs. See copyright credits on Impulse! CD back cover on Discogs. See credits on the ... On Compact Discs issued by Charly and Affinity the concert had an extra track as opener, "How High the Moon". In 1989 MCA ... through which it also appeared on Affinity in the UK. All releases were distributed with the same front and back cover. ... finally released the album in the US on their Impulse! label, but omitting "How High the Moon" on CD as well. When Bridgewater ...

... received a PhD in chemistry from Georgia Tech in 1978; his thesis was entitled "Affinity Labeling of ... Rasnick, David William (1978). Affinity labeling of metalloendoproteases (electronic thesis or dissertation). OCLC 638449874. ...

Her 1971 dissertation was titled, Affinity labelling lysozyme with a carbene. She earned a M.P.H. from Harvard T.H. Chan School ... Ziegler, Regina Gale (1971). Affinity labelling lysozyme with a carbene (Ph.D. thesis). University of California, Berkeley. ...

It was released in 1981 on the Affinity label. The same recordings were officially released in 2021 in cooperation with the ...

Inglese J, Johnson DL, Shiau A, Smith JM, Benkovic SJ (February 1990). "Subcloning, characterization, and affinity labeling of ... Using a bromoacetyl dideazafolate affinity analog James Inglese and colleagues first identified Asp144 as an active site ...

Affinity purification Protein array TimeSTAMP protein labelling Western blotting Mahmoudi Gomari, Mohammad; Saraygord-Afshari, ... Affinity tags are appended to proteins so that they can be purified from their crude biological source using an affinity ... Affinity tags include chitin binding protein (CBP), maltose binding protein (MBP), Strep-tag and glutathione-S-transferase (GST ... "CaptureSelect C-tag Affinity Matrix - Thermo Fisher Scientific". www.thermofisher.com. Cooper, Merideth A.; Taris, Joseph E.; ...

"Label-free microscale thermophoresis discriminates sites and affinity of protein-ligand binding". Angew. Chem. Int. Ed. Engl. ... The thermophoresis of a fluorescently labeled molecule A typically differs significantly from the thermophoresis of a molecule- ... The thermophoretic movement of the fluorescently labelled molecule is measured by monitoring the fluorescence distribution F ... change of the fluorescence detected when a temperature gradient is applied and can be used to determine binding affinities. MST ...

Other labels can be used, such as affinity, photochemical or radioisotope tags. These labels are attached to the probe itself ... Probe molecules, typically labeled with a fluorescent dye, are added to the array. Any reaction between the probe and the ... All these label free detection methods are relatively new and are not yet suitable for high-throughput protein interaction ... The advantages of controlling the precise mode of surface attachment through use of an appropriate affinity tag are that the ...

Ligand conjugation is targeted to specific lysine residues via an affinity labeling mechanism. There has been a reluctance for ... The design of covalent drugs requires careful optimization of both the non-covalent binding affinity (which is reflected in Ki ... Ostrem JM, Peters U, Sos ML, Wells JA, Shokat KM (November 2013). "K-Ras(G12C) inhibitors allosterically control GTP affinity ... Smith AJ, Zhang X, Leach AG, Houk KN (January 2009). "Beyond picomolar affinities: quantitative aspects of noncovalent and ...

Ratajczak T, Sheppard PN, Capon RJ, Hähnel R (November 1981). "The synthesis and study of some potential affinity labeling ... "A comparison of 11 beta-chloromethylestradiol and tamoxifen aziridine as affinity labeling reagents for estrogen receptors". ... The relative binding affinity of 11β-CME2 for the estrogen receptors ranges from 230 to 3,320% of that of estradiol depending ... It has very high affinity for the estrogen receptor and dissociates from it relatively slowly. It was originally thought that ...

The film was distributed internationally by Sierra/Affinity, a label of Entertainment One. Orphan: First Kill was released ... The project was announced as a joint-venture production between eOne, Dark Castle Entertainment, Sierra/Affinity, and Eagle ... by Paramount Pictures through the Paramount Players label in select theatres, digital, and streaming via Paramount+. The film ...

Maryanoff BE, Simon EJ, Gioannini T, Gorissen H (August 1982). "Potential affinity labels for the opiate receptor based on ... Maryanoff BE, Simon EJ, Gioannini T, Gorissen H (August 1982). "Potential affinity labels for the opiate receptor based on ... Essawi MY (April 1999). "Fentanyl analogues with a modified propanamido group as potential affinity labels: synthesis and in ...

Uram JD, Ke K, Hunt AJ, Mayer M (March 2006). "Label-free affinity assays by rapid detection of immune complexes in ... Gao QH, Zhang WM, Zou HX, Li WB, Yan H, Peng ZK, Meng G (2019). "Label-free manipulation via the magneto-Archimedes effect: ... Other research has also shown that the label-free separation of cells may be possible by suspending cells in a paramagnetic ... Akiyama Y, Morishima K (2011-04-18). "Label-free cell aggregate formation based on the magneto-Archimedes effect". Applied ...

"Inactivation of Escherichia coli outer-membrane phospholipase A by the affinity label hexadecanesulfonyl fluoride. Evidence for ...

... a specific high affinity ligand for labeling the dopamine transport complex". European Journal of Pharmacology. 107 (2): 289-90 ... and oxo substituents on affinity for the dopamine and serotonin transporters". Journal of Medicinal Chemistry. 51 (9): 2795-806 ... 3H radiolabelled form for the purpose of mapping the distribution of dopaminergic neurons in the brain by selective labelling ...

Affinity labelling of the 40S subunits by derivatives of oligoribonucleotides containing the AUG codon". Biochim. Biophys. Acta ...

Affinity labelling of the 40S subunits by derivatives of oligoribonucleotides containing the AUG codon". Biochim. Biophys. Acta ...

Affinity labelling of the 40S subunits by derivatives of oligoribonucleotides containing the AUG codon". Biochim. Biophys. Acta ...

Isotope-coded affinity tag (ICAT), label-free) and qualitative. In collaboration with scientists at the University of Dundee, ...

"Solution-Phase vs Surface-Phase Aptamer-Protein Affinity from a Label-Free Kinetic Biosensor". PLOS ONE. 8 (9): e75419. Bibcode ... which prove to get the advantages of both label-based and label-free detection methods in the single setup. SPR imaging is used ... Affinity interactions can be studied for a variety of target molecules e.g. proteins and nucleic acids. Mismatching of bases in ... Although it's label free but will need to have crystal clean experimental conditions. Sensitivity of SPRM can be improved with ...

Affinity labelling of the 40S subunits by derivatives of oligoribonucleotides containing the AUG codon". Biochim. Biophys. Acta ...

... a high affinity ligand and novel photoaffinity labeling reagent for the progesterone receptor". J. Steroid Biochem. 35 (2): 179 ... The affinity of DU-41164 for the progesterone receptor was described in 1974 as "probably the highest reported for any steroid- ... The drug shows extremely high potency as a progestogen in animals; it was reported to possess 500 times the affinity of ... A closely related compound, DU-41165, has been developed as a photoaffinity label for the progesterone receptor. Halkes SJ, ...

As affinity probes in CAE, fluorophore-labeled compounds with affinities for the target molecules are employed. With ACE, ... The proteins that do not have affinity for the affinity probes pass through the affinity-trap gel, and proteins with affinity ... These receptor molecules often take the form of affinity probes consisting of fluorophore-labeled molecules that will bind to ... Phosphate affinity electrophoresis utilizes an affinity probe which consists of a molecule that binds specifically to divalent ...

Murale DP, Hong SC, Haque MM, Lee JS (2017-06-24). "Photo-affinity labeling (PAL) in chemical proteomics: a handy tool to ... In a label transfer reaction, a known protein is tagged with a detectable label. The label is then passed to an interacting ... Affinity electrophoresis as used for estimation of binding constants, as for instance in lectin affinity electrophoresis or ... Label transfer can also detect weak or transient interactions that are difficult to capture using other in vitro detection ...

Affinity labeling of an active site lysine by pyridoxal 5'-phosphate and pyridoxal 5'-diphospho-5'-adenosine". The Journal of ...

... for affinity chromatography, affinity labeling, affinity therapy, and the avidin-biotin system. The avidin-biotin complex is ... Specific uses of affinity chromatography include antibody affinity, Immobilized metal ion affinity chromatography and ... Strausbauch, P.H., Weinstein, Y., Wilchek, M., Shaltiel, S., Givol, D. (1971). "A homologous series of affinity labeling ... thus laying the grounds for modern hemoperfusion Affinity label is a molecule that is similar in structure to a particular ...

Affinity label for the tRNA binding sites on the E. coli ribosome allowed the identification of A and P site proteins most ... During 1977, Czernilofsky published research that used affinity labeling to identify tRNA-binding sites on rat liver ribosomes ... "The site of reaction on ribosomal protein L27 with an affinity label derivative of tRNA Met f". FEBS Letters. 63 (2): 283-6. ... likely associated with the peptidyltransferase activity; labelled proteins are L27, L14, L15, L16, L2; at least L27 is located ...