... is the ex vivo culture of cells freshly obtained from a multicellular organism, as opposed to the culture ... More specific terms include organotypic culture, tissue slices and explants. Neuronal primary cell cultures are cells collected ... primary cell cultures did not become widespread until the 2000s. These cultures present several advantages over cell lines, ... In general, primary cell cultures are considered more representative of in vivo tissues than cell lines, and this is recognized ...
ISBN 978-0-471-19350-0. Schantz JT, Ng KW (2004). A manual for primary human cell culture. World Scientific. p. 89. Comroe JH ( ... Phelan K, May KM (March 2015). "Basic techniques in mammalian cell tissue culture". Current Protocols in Cell Biology. 66 (1): ... is used in a standard antibiotic cocktail to prevent bacterial infection in cell culture. When purifying protein from a ... This results in an unstable ribosomal-mRNA complex, leading to premature stopping of protein synthesis, leading to cell death. ...
Stem cells are cells in the body that can divide indefinitely in a culture and can be formulated into specialized cells. In ... This range of yield is expected for the expansion of primary cells. We isolated these cells from the marrow of over 350 donors ... "about cell type gene expression, cell lineage maps and stem cell differentiation protocols for both human and mouse stem cells ... In the expanded cultures, hematopoietic stem cells were not found. The mesenchymal cells appeared to be different from marrow ...
1989). "Secondary metabolites in cell cultures of Teabernaemontana species". In Kurz, Wolfgang G. W. (ed.). Primary and ... In cell cultures, it has shown cytotoxicity against the experimental lymphocytic leukemia P388 cell line. It exhibits strong ... Secondary Metabolism of Plant Cell Cultures II. Springer-Verlag. pp. 138-148. doi:10.1007/978-3-642-74551-5_16. ISBN 978-3-642- ...
These cells may be cells isolated from a donor organism (primary cells) or an immortalised cell line. The cells are bathed in a ... Cancer Cells in Culture Evolution of Cell Culture Surfaces Hypertext version of the Cell Line Data Base Cell Culture ... Plant cell lines Tobacco BY-2 cells (kept as cell suspension culture, they are model system of plant cell) Other species cell ... A cell strain is derived either from a primary culture or a cell line by the selection or cloning of cells having specific ...
Listed below are the cell types (primary and cell lines) that have been successfully cultured by the magnetic levitation method ... 3D cell culturing is scalable, with the capability for culturing 500 cells to millions of cells or from single dish to high- ... Because of the ability of 3D cell culturing by magnetic levitation to bring cells together, co-culturing different cell types ... Standard monolayer cell culturing on tissue culture plastic has notably improved our understanding of basic cell biology, but ...
examined one particular cell type, neurons (from primary neuron cultures). They exhibited 28,492 putative enhancers generating ... May 2007). "High-resolution profiling of histone methylations in the human genome". Cell. 129 (4): 823-837. doi:10.1016/j.cell. ... Cell. 121 (5): 725-737. doi:10.1016/j.cell.2005.04.030. PMID 15935759. Ren B (May 2010). "Transcription: Enhancers make non- ... used these cultured neurons to examine the timing of specific enhancer eRNAs compared to the mRNAs of their target genes. The ...
"Prolific Replication of HIV-1 in Primary Dendritic Cell Cultures". Proceedings of the National Academy of Sciences. 88 (18): ... After he was briefed on the project to isolate human embryonic stem cells and embryonic germ cells at Geron Corporation in ... Together with Healthcare Ventures, Haseltine formed the company Activated Cell Therapy Inc. to use dendritic cells to treat ... "Development of Methotrexate Resistance in a Human Squamous Cell Carcinoma of the Head and Neck in Culture". Proceedings of the ...
"Interleukin 4 induces cultured monocytes/macrophages to form giant multinucleated cells". Primary. The Journal of Experimental ... Eukaryotic cells usually have a single nucleus, but a few cell types, such as mammalian red blood cells, have no nuclei, and a ... Other multinucleate cells in the human are osteoclasts a type of bone cell. Multinucleated and binucleated cells can also be ... the cell divides to form two cells. In order for this process to be possible, each of the new daughter cells must have a full ...
Evidence from cell lines and primary breast cancer cultures in vitro". Angiogenesis. 8 (3): 197-204. doi:10.1007/s10456-005- ... Paracrine signaling is a form of cell-cell communication in which a cell produces a signal to induce changes in nearby cells, ... These T cells can then go on to perform effector functions such as macrophage activation, B cell activation, and cell-mediated ... When interleukin-1 is produced in response to external stimuli, it can bind to cell-surface receptors on the same cell that ...
Common types of 2D cell culture used to model Alzheimer's disease are immortalized cell lines, primary neuron cultures, and ... traditional 2D cell culture, 3D cell culture, microphysiological systems, and animal models. Traditional two dimensional cell ... These cultures occur on a dish or flask in a monolayer and can be made up of a single cell type or multiple cell types. 2D ... Primary neuron cultures are generated from embryonic or postnatal rodent brain tissue and cultured on plates. Common brain ...
Lowy, R.J.; Dawson, D.C.; Ernst, S.A. (1989). "Mechanism of ion transport by avian salt gland primary cell cultures". American ... These cells are similar to the mitochondria rich cells found in teleost fish. These cells within the salt gland employ several ... triangular shaped cells which have only a few mitochondria. The second specialized cells are the principal cells which are ... The ATPase uses energy from ATP to pump three sodium ions out of the cell and two potassium ions into the cell. The potassium ...
White AL, Lanford RE (November 1994). "Cell surface assembly of lipoprotein(a) in primary cultures of baboon hepatocytes". The ... which attracts inflammatory cells to vessel walls and leads to smooth muscle cell proliferation. Moreover, Lp(a) also is ... Lp(a) is not fully synthesised until the precursor protein is released from the cell, so the slower rate of production for the ... This inhibition of PLG by Lp(a) also promotes the proliferation of smooth muscle cells. These unique features of Lp(a) suggest ...
... and Primary Cell Cultures". JNCI: Journal of the National Cancer Institute. 28: 147-157. doi:10.1093/jnci/28.1.147. PMID ... Crowell, Richard L.; Syverton, Jerome T. (1961). "The Mammalian Cell-Virus Relationship. VI. Sustained Infection of HeLa Cells ... "Isolation and Elimination of Pleuropneumonia-Like Organisms from Mammalian Cell Cultures". Experimental Biology and Medicine. ... over 1300 citations) Scherer, W. F.; Syverton, J. T. (1954). "The viral range in vitro of a malignant human epithelial cell ( ...
2020-04-03). "Three-Dimensional Primary Cell Culture: A Novel Preclinical Model for Pancreatic Neuroendocrine Tumors". ... together with nearby cells, are damaged by the attached radiation. Not all cells are immediately killed; cell death can go on ... small-cell lung cancer (SCLC) large cell neuroendocrine carcinoma of the lung (LCNEC) Extrapulmonary small cell carcinomas ( ... Histologically, NETs are an example of "small blue cell tumors," showing uniform cells which have a round to oval stippled ...
"Establishment of a primary cell culture from a sponge:primmorphs from Suberites domuncula". Marine Ecology Progress Series. 178 ... This species also inhabits Wnt signaling, which is responsible for cell communication, specifically cell-fate decisions, tissue ... This species is made up of archeocytes and choanocytes, which act as the stem cells to the sponge. Suberites domuncula are able ... Dissociated single cells from Suberites domuncula form multicellular aggregates, known as primmorphs. These primmorphs are ...
"On the use of neuro-2a neuroblastoma cells versus intact neurons in primary culture for neurotoxicity studies". Critical ... N2a cells (also known as Neuro2a cells) are a fast-growing mouse neuroblastoma cell line. Originating from a mouse, the N2a ... in N2a cells, a major contributor to N2a cell differentiation. N2a cells are able to rapidly, reliably, and easily ... The cells, due to passaging since initial collection, can exhibit responses to toxins that differ from those of neuronal cells ...
"Obtaining of measles virus haemagglutinin from strain L-16 grown in primary cell cultures". Acta Virologica. 19 (6): 497. ISSN ... Growing up on Manhattan's Upper East Side, Frankenthaler absorbed the privileged background of a cultured and progressive ... culture wars' that eventually led to deep budget cuts for the National Endowment for the Arts and a ban on grants to individual ... New York City Mayor's Award of Honor for Arts and Culture (1986); and Distinguished Artist Award for Lifetime Achievement, ...
"The bZIP transcription factor Nrl stimulates rhodopsin promoter activity in primary retinal cell cultures". The Journal of ... The encoded protein is conserved among vertebrates and is a critical intrinsic regulator of photoreceptor cell development and ... binds to and transactivates photoreceptor cell-specific genes". Neuron. 19 (5): 1017-30. doi:10.1016/S0896-6273(00)80394-3. ...
She was one of the first scientists in Hungary to detect pathogenic virus in primary cell cultures. She led the Institute until ... Simonyi, Erzsébet (1963). "Isolation of Rubarth's Virus from Primary Dog Kidney Tissue Culture Cells". Acta veterinaria. ... Simonyi, Erzsébet (1963). "Titration of Antiviral Sera of Monolayer Tissue Cultures of Kidney Cells". Acta veterinaria. ... Measurement of Antiviral Serum Values in Monolayer Kidney Cell Culture]. Magyar állatorvosok lapja (in Hungarian). Budapest: ...
"Nonviral in situ green fluorescent protein labeling and culture of primary, adult human hair follicle epithelial progenitor ... Stem cell markers are genes and their protein products used by scientists to isolate and identify stem cells. Stem cells can ... 2005). "Somatic stem cell marker prominin-1/CD133 is expressed in embryonic stem cell-derived progenitors". Stem Cells. 23 (6 ... Hirao A, Arai F, Suda T (December 2004). "Regulation of cell cycle in hematopoietic stem cells by the niche". Cell Cycle. 3 (12 ...
Harovltch, Sharon (1975). "Myogenesis in primary cell cultures from Drosophila melanogaster: protein synthesis and actin ... If placed in cell culture, most myoblasts will proliferate if enough fibroblast growth factor (FGF) or another growth factor is ... In addition, satellite cells have the capability to also differentiate into bone or fat. In this way, satellite cells have an ... Cells that make myogenic bHLH transcription factors (including MyoD or Myf5) are committed to development as a muscle cell. ...
The primary purpose behind the entrepreneurship cell was to accelerate the entrepreneurial culture at ICT. ICT also has ... "E-Cell ICT Mumbai - A Next Generation Entrepreneurship Initiative". ecellict.com. Retrieved 9 April 2022. BioPrepWatch Reports ... ICT inaugurated its entrepreneurship cell in April 2013 with an inauguration lecture from Ashwin Dani (Founder of Asian Paints ... MHRD's Innovation Cell (MIC), Ministry of Education, Govt. of India. p. 7. Retrieved 6 October 2020. "New method developed to ...
... adherent mammalian cell lines and primary cell cultures show very high transfection rates. Suspension cells and cells from ... "Successfully Transfected Cells". IBA Solutions for Life Sciences. Archived from the original on February 23, 2012. Davis, M. E ... The magnetic field causes the iron particles to be rapidly drawn towards the surface of the cell membrane. Cellular uptake ... Magnet-assisted transfection is a relatively new and time-saving method to introduce nucleic acids into a target cell with ...
"Efficient gene disruption in cultured primary human endothelial cells by CRISPR/Cas9". Circulation Research. 117 (2): 121-8. ... He hired slowly, knowing that each new person would have a significant effect on the overall department culture. Saltzman ... cell interactions with polymers, and tissue engineering. He studies how to create safer and more effective medical and surgical ... Saltzman built scaffolds that could be seeded with cells to sculpt new replacement tissues. He also created drug-impregnated ...
"Regulation of pepsinogen release from canine chief cells in primary monolayer culture". The American Journal of Physiology. 245 ... It also has the function of stimulating pepsinogen secretion by chief cells. VIP seems to be an important neuropeptide during ... The SCN coordinates daily timekeeping in the body and VIP plays a key role in communication between individual brain cells ... The ability of the population to remain synchronized as well as the ability of single cells to generate oscillations is ...
... it is oncogenic in some rodents and can immortalize some human cells in primary cell culture. SV40 has three early proteins, ... abrogating either binding site renders LTag unable to transform primary cultured cells. In fact, p53 - now established as a key ... the cell must be in S phase (the part of the cell cycle in which the host cell's genome is normally replicated) in order to ... The major functions of LTag in the viral life cycle involve dysregulation of the host cell's cell cycle and replication of the ...
Cellular Effects of T-2 Toxin on Primary Hepatic Cell Culture Models of Chickens. Toxins 2020, 12, 46. https://doi.org/10.3390/ ... In red blood cells T-2 mycotoxin is metabolized to neosolaniol, and, in white blood cells, to HT-2 via hydrolysis catalyzed by ... Furthermore, the level of Fas, an apoptosis-related cell-surface antigen and p53, a protein regulating the cell cycle, were ... in a study carried out in different chicken derived hepatic cell culture models, no alterations were found in the redox status ...
... identification of the cell types initially infected during primary transmission in humans; and the connection between viral and ... Lytic growth of Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) in culture. Nature Medicine. 1996;2(3):342-346. ... Hassman, L.H., Ellison, T.J., Kedes, D.H. KSHV infects a subset of human tonsillar B cells, driving proliferation and ... Progression through the Lytic Cascade and Variations in Intracellular Viral Loads Revealed by High-Throughput Single-Cell ...
"Characterization of viral particles isolated from primary cultures of human breast cancer cells". Cancer Research. 67 (18): ...