Plasmids can be broadly classified into conjugative plasmids and non-conjugative plasmids. Conjugative plasmids contain a set ... Many plasmids have been created over the years and researchers have given out plasmids to plasmid databases such as the non- ... Virulence plasmids, which turn the bacterium into a pathogen. e.g. Ti plasmid in Agrobacterium tumefaciens Plasmids can belong ... Plasmids are generally circular, but examples of linear plasmids are also known. These linear plasmids require specialized ...
These were all separate isolates, and later found to be identical plasmids. The IncP-1 plasmid group (IncP plasmids in ... The RK2 Plasmid is a broad-host-range plasmid belonging to the incP incompatibility group It is notable for its ability to ... Minimal plasmids such as PFF1 are useful for studying the basic mechanisms of plasmid replication and copy number regulation, ... Plasmid, 2010 Nov;64(3):119-34 U Kües and U Stahl: "Replication of plasmids in gram-negative bacteria", Microbiol Rev. 1989 ...
... is a non-coding RNA found in bacterial plasmids including pIP501. RNAIII acts by transcriptional attenuation of ... Page for Plasmid RNAIII at Rfam v t e (Non-coding RNA, All stub articles, Molecular and cellular biology stubs). ... importance of a U-turn loop structure in the target RNA of plasmid pIP501 for efficient inhibition by the antisense RNA". ...
... is a plasmid that contains an inserted piece of foreign DNA. "Everything Bio : Hybrid plasmid". Everythingbio. ... v t e (Articles needing additional references from November 2013, All articles needing additional references, Plasmids, All ...
... and purification of the plasmid DNA. Purification of plasmids is central to molecular cloning. A purified plasmid can be used ... A typical plasmid DNA yield of a miniprep is 5 to 50 µg depending on the cell strain. Miniprep of a large number of plasmids ... A plasmid preparation is a method of DNA extraction and purification for plasmid DNA, it is an important step in many molecular ... The plasmid DNA yield will vary depending on the plasmid copy number, type and size, the bacterial strain, the growth ...
The R1 plasmid is a plasmid that was first isolated from Salmonella paratyphi bacteria in 1963. It is a short plasmid, composed ... Nordström, Kurt (2006). "Plasmid R1--replication and its control". Plasmid. 55 (1): 1-26. doi:10.1016/j.plasmid.2005.07.002. ... The R1 plasmid partitioning is a mechanism needed for the inheritance of the R1 plasmid. The par system is composed of the ParR ... The R1 plasmid imparts multi-drug antibiotic resistance to its host bacteria. It's known as a "low copy" plasmid, meaning that ...
While all types of Ri-plasmid contain T-DNA, the agropine plasmid contains both a TL and a TR-domain (left and right, resp.). ... The root inducing (Ri) -plasmid of Rhizobium rhizogenes (formerly Agrobacterium rhizogenes) is a plasmid capable of undergoing ... The Ri-plasmid is generally classified based on the type of opines produced, and four have been described so far: the agropine ... The T-DNA of the Ri-plasmid affects the plant host in such a way, that gene expression is altered, especially in regard to ...
The Ti plasmid is a member of the RepABC plasmid family found in Alphaproteobacteria. These plasmids are often relatively large ... the Ti plasmid is part of a family of plasmids carried by many species of Alphaproteobacteria. Members of this plasmid family ... Some notable early milestones in the studies of Ti plasmids include the mapping of a Ti plasmid in 1978 and the studying of ... virF is a host specificity factor found in some but not all types of Ti plasmids; for example, octopine-type Ti plasmids ...
The F plasmid belongs to F-like plasmids, a class of conjugative plasmids that control sexual functions of bacteria with a ... The F-plasmid belongs to a class of conjugative plasmids that control sexual functions of bacteria with a fertility inhibition ... F+ bacteria possess F factor as a plasmid independent of the bacterial genome. The F plasmid contains only F factor DNA and no ... The F factor was the first plasmid to be discovered. Unlike other plasmids, F factor is constitutive for transfer proteins due ...
... is the transfer of antibiotic resistance genes which are carried on plasmids. Plasmids possess ... Study investigating physiological effect of pHK01 plasmid in host E.coli J53 found that the plasmid reduced bacterial motility ... In Enterobacteriaceae, 28 different plasmid types can be identified by PCR-based replicon typing (PBRT).The plasmids that have ... According to that, Mobilizable plasmids are smaller in size (usually < 10 kb) while conjugative plasmids are largger (usually ...
Each plasmid has its independent replication system which controls the number of copies of the plasmid in a cell. The higher ... pSK1 is a plasmid from Staphylococcus aureus. This plasmid has a partition system determined by a single gene, par, previously ... A plasmid partition system is a mechanism that ensures the stable inheritance of plasmids during bacterial cell division. ... For instance, if there are 2 copies of a plasmid in a cell, there is 50% chance of having one plasmid-less daughter cell. ...
When the plasmid concentration is high, RepA plasmids bound to iterons form dimers in between two plasmids, "handcuffing" them ... Low copy number plasmids, also called stringent plasmids, require tighter control of replication. In ColE1 derived plasmids, ... of the plasmid). This is particularly likely with low copy number plasmids. Plasmids can also be incompatible due to shared ... In R1 plasmids RepA can be transcribed from two different promoters. It is made from the first promoter until the plasmid ...
The Bacillus-plasmid RNA motif is a predicted conserved RNA structure usually located in plasmids. It is known in species under ... The fact that the RNA structure is typically found in plasmids suggests that it might be involved in the regulation plasmid ... However, the Bacillus-plasmid RNA motif does not appear to be homologous to any such known RNA. Weinberg Z, Wang JX, Bogue J, ... Page for Bacillus-plasmid RNA at Rfam v t e (Orphaned articles from December 2021, All orphaned articles, Non-coding RNA, All ...
However, many of the plasmids that carry lactis-plasmid RNAs also carry ctRNA-pND324 RNAs, which are involved in plasmid copy ... Therefore lactis-plasmid RNAs might have a different function. R1162-like plasmid antisense RNA Weinberg Z, Wang JX, Bogue J, ... They typically lie near to repB genes, and are almost found in plasmids. This data suggested that lactis-plasmid RNAs ... The lactis-plasmid RNA motif is a conserved RNA structure identified by bioinformatics. The RNAs are restricted to lactic acid ...
Human HGF plasmid DNA therapy of cardiomyocytes is being examined as a potential treatment for coronary artery disease (a major ... Transfection with HGF plasmids in damaged cardiac tissue also promotes angiogenesis (increased capillary density compared to ... Animal research has demonstrated that administration of HGF cDNA plasmids into ischemic cardiac tissue can increase cardiac ... "Enhanced cardioprotective effects by coexpression of two isoforms of hepatocyte growth factor from naked plasmid DNA in a rat ...
... is a 75-base RNA molecule which negatively regulates the RepI region of the plasmid. The ... "Copy number of the broad host-range plasmid R1162 is determined by the amounts of essential plasmid-encoded proteins". Journal ... Kim K, Meyer RJ (October 1986). "Copy-number of broad host-range plasmid R1162 is regulated by a small RNA". Nucleic Acids ... Page for R1162-like plasmid antisense RNA at Rfam v t e (Antisense RNA, All stub articles, Molecular and cellular biology stubs ...
Smaller independent pieces of DNA, called plasmids, are also found in archaea. Plasmids may be transferred between cells by ... Sota M, Top EM (2008). "Horizontal Gene Transfer Mediated by Plasmids". Plasmids: Current Research and Future Trends. Caister ... ISBN 978-1-904455-27-1. Lipps G (2008). "Archaeal Plasmids". Plasmids: Current Research and Future Trends. Caister Academic ... Schleper C, Holz I, Janekovic D, Murphy J, Zillig W (August 1995). "A multicopy plasmid of the extremely thermophilic archaeon ...
Plasmids containing the Factor IX gene, along with plasmids with a gene that codes for resistance to methotrexate, were ... The recombinant plasmids were inserted into Escherichia coli bacteria, which were "induced to produce 100,000 molecules of ... The gene that was inserted into the plasmid was created by reverse transcription of the mRNA found in pituitary glands to ... The artificial genes were "then inserted... into plasmids... among a group of genes that" are activated by lactose. Thus, the ...
"ABOUT THE WRITER". GENTLE PLASMIDS. JY Yang. 12 January 2017. Archived from the original on 21 February 2018. Retrieved 20 ...
It is used to increase the transfection efficiency of RNA (including mRNA and siRNA) or plasmid DNA into in vitro cell cultures ... DNA plasmids. Lipofectamine consists of a 3:1 mixture of DOSPA (2,3‐dioleoyloxy‐N‐ [2(sperminecarboxamido)ethyl]‐N,N‐dimethyl‐1 ...
Cells harboring the plasmid will survive when exposed to the antibiotic, while those that have failed to take up plasmid ... "plasmid / plasmids , Learn Science at Scitable". www.nature.com. Retrieved 2017-12-06. Shizuya H, Birren B, Kim UJ, Mancino V, ... and a plasmid cloning vector. E. coli and plasmid vectors are in common use because they are technically sophisticated, ... That is, these plasmids could serve as cloning vectors to carry genes. Virtually any DNA sequence can be cloned and amplified, ...
Fertility plasmids, or f plasmids, allow for conjugation to occur whereas resistance plasmids, or r plasmids, contain genes ... Circular bacterial plasmids are classified according to the special functions that the genes encoded on the plasmid provide. ... Circular bacterial plasmids are also the basis for the production of DNA vaccines. Plasmid DNA vaccines are genetically ... The linear plasmids with a covalently attached protein may assist with bacterial conjugation and integration of the plasmids ...
Plasmids in Bacteria. Boston, MA: Springer US. ISBN 1-4613-2447-5. {{cite book}}: ,first1= has generic name (help)CS1 maint: ...
The G+C base content of the DNA is 46.6%. T. petrophila is also known to contain one of the smallest plasmids. Thermotoga ... "Mobility of plasmids". Microbiology and Molecular Biology Reviews. 74 (3): 434-452. doi:10.1128/MMBR.00020-10. PMC 2937521. ... petrophila RKU1 plasmid (pRKU1) is negatively supercoiled, contains 846 base pairs, and carries only the rep gene. Due to T. ...
As of 2014 Addgene's repository comprised 30,000 plasmids, deposited by 1,700 labs. Its plasmid collection contains plasmids ... Plasmid Cloning Guides Molecular Cloning Guides-References to help scientists design plasmid cloning experiments, including ... The plasmids are available to both academic and industry labs. In 2020, Addgene received funding from Fast Grants to subsidize ... Addgene accepts plasmids from researchers for distribution and archival. Addgene obtains revenues and licensing fees using the ...
Plasmids in Bacteria. Boston, MA: Springer US. ISBN 978-1-4613-2447-8. {{cite book}}: ,first1= has generic name (help)CS1 maint ...
Kandavelou K, Chandrasegaran S (2008). "Plasmids for Gene Therapy". In Lipps, Georg (ed.). Plasmids: Current Research and ...
Kandavelou K, Chandrasegaran S (2008). "Plasmids for Gene Therapy". Plasmids: Current Research and Future Trends. Norfolk: ... Since ZFN-encoding plasmids could be used to transiently express ZFNs to target a DSB to a specific gene locus in human cells, ... The ZFN-encoding plasmid-based approach has the potential to circumvent all the problems associated with the viral delivery of ... The construction of plasmid vectors is simple and straightforward. Custom-designed ZFNs that combine the non-specific cleavage ...
The T-DNA must be cut out of the circular plasmid. This is typically done by the Vir genes within the helper plasmid. A VirD1/ ... The two plasmids are pTiC58, responsible for the processes involved in virulence, and pAtC58, once dubbed the "cryptic" plasmid ... "At plasmid" when talking about related plasmids Smith EF, Townsend CO (April 1907). "A Plant-Tumor of Bacterial Origin". ... To be virulent, the bacterium contains a tumour-inducing plasmid (Ti plasmid or pTi) 200 kbp long, which contains the T-DNA and ...
"Geraldine Seydoux Lab Plasmids". Addgene. (Articles with short description, Short description matches Wikidata, Articles with ...