Microinjection
... can also be used in the cloning of organisms, in the study of cell biology and viruses, and for treating male ... Microinjection is the use of a glass micropipette to inject a liquid substance at a microscopic or borderline macroscopic level ... Microinjection is a simple mechanical process usually involving an inverted microscope with a magnification power of around ... There are two basic types of microinjection systems. The first is called a constant flow system and the second is called a ...
Micro injection molding
"Micro Injection Moulding Market: A phenomenal growth in the coming years". Micro Systems. Retrieved May 22, 2023. "Micro ... The most common polymers used in micro injection molding are reported in the table below: In the 1980s, micro injection molding ... Micro injection molding is a molding process for the manufacture of plastics components for shot weights of 1 to 0.1 grams with ... The micro injection molding plastic market was valued at $1,145.85 million in 2022 and is anticipated to reach $2,640 million ...
Clytia hemisphaerica
Stepicheva, Nadezda A.; Song, Jia L. (2014-01-21). "High Throughput Microinjections of Sea Urchin Zygotes". Journal of ...
Helen Sang
Sang, Helen (1994). "Transgenic Birds by DNA Microinjection". EMBO Reports. 12 (1): 60-63. doi:10.1038/nbt0194-60. PMID 7764327 ...
Nanoinjection
Microinjection is the predecessor to nanoinjection. Still used in biological research, microinjection is useful in the ... It is claimed to be more effective than microinjection because the lance used is ten times smaller than a micropipette and the ... Just as with the other methods, a lance ten times smaller than that of microinjection is used. Preparing the lance for ... As opposed to nanoinjection, microinjection uses DNA-filled liquid driven into the cell under pressure. Depending on factors ...
R. Marthanda Varma
Intracellular delivery
... methods that utilize direct penetration include: Classic microinjection Newer forms of microinjection (e ... For a century microinjection has been the dominant method for introducing microscale cargo into cells. A classic example was ... Hiramoto, Y. (1962). "Microinjection of Live Spermatozoa into Sea Urchin Eggs". Exp. Cell Res. 27 (3): 416−426. doi:10.1016/ ... Plasmid DNA began to be transfected into animal cells for the purpose of gene expression in the late 1970s via microinjection ...
Transcription activator-like effector
August 2011). "Knockout rats generated by embryo microinjection of TALENs". Nature Biotechnology. 29 (8): 695-6. doi:10.1038/ ...
Humboldt squid
Rojas, & Luxoro (1963). "Micro-injection of Trypsin into Axons of Squid". Nature. 199 (4888): 78-79. Bibcode:1963Natur.199... ...
Transcription activator-like effector nuclease
This approach has also been used to generate knockin rats by TALEN mRNA microinjection in one-cell embryos. TALEN has also been ... "Site specific mutation of the Zic2 locus by microinjection of TALEN mRNA in mouse CD1, C3H and C57BL/6J oocytes". PLOS ONE. 8 ( ... "Knockout rats generated by embryo microinjection of TALENs". Nature Biotechnology. 29 (8): 695-6. doi:10.1038/nbt.1940. PMID ...
Morpholino
A microinjection apparatus is usually used for delivery into an embryo, with injections most commonly performed at the single- ... Rosen JN, Sweeney MF, Mably JD (March 2009). "Microinjection of zebrafish embryos to analyze gene function". Journal of ... as demonstrated by the nuclear splice-modifying activity of Morpholinos observed after microinjection into the cytosol of cells ...
Zebrafish
Rosen JN, Sweeney MF, Mably JD (March 2009). "Microinjection of zebrafish embryos to analyze gene function". Journal of ...
Knockout rat
Standard microinjection techniques allow this technology to make knockout rats in 4-6 months. A major advantage of ZFN- and ... al, Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases. Science. Vol 325: 433 (24 July 2009) Geurts, A. M.; Cost ... Tesson et al., Knockout rats generated by embryo microinjection of TALENs. Nature Biotechnology Vol 29:695-96 (5 August 2011) ... "Knockout rats generated by embryo microinjection of TALENs". Nature Biotechnology. 29 (8): 695-696. doi:10.1038/nbt.1940. PMID ...
Nucleus raphe obscurus
It has also been shown that this nucleus stimulates gastrointestinal motor function; microinjections of 5-HT into the nucleus ...
Recombinant AAV mediated genome engineering
July 2009). "Knockout rats via embryo microinjection of zinc-finger nucleases". Science. 325 (5939): 433. Bibcode:2009Sci...325 ...
Restriction enzyme
July 2009). "Knockout rats via embryo microinjection of zinc-finger nucleases". Science. 325 (5939): 433. Bibcode:2009Sci...325 ...
Zinc finger nuclease
"Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases". Science. 325 (5939): 433. Bibcode:2009Sci...325..433G. doi: ...
Marshall A. Barber
He proposed the technique of microinjection to clone bacteria. He developed micropipette methods in 1904 for microscopic renal ... Korzh, Vladimir; Strähle, Uwe (2002-08-01). "Marshall Barber and the century of microinjection: from cloning of bacteria to ...
Maria Byrne (biologist)
Edgar, Allison; Byrne, Maria; Wray, Gregory A. (17 September 2019). "Embryo microinjection of the lecithotrophic sea urchin ...
Keith Burridge
Feramisco, JR (1979). "Microinjection of fluorescently labeled alpha-actinin into living fibroblasts". Proceedings of the ... Burridge K, Feramisco JR; Feramisco (March 1980). "Microinjection and localization of a 130K protein in living fibroblasts: a ...
History of genetic engineering
... micro-injection and particle bombardment with a gene gun (invented in 1987). In the 1980s techniques were developed to ... Agrobacterium-mediated recombination and microinjection. The first genetically modified animal was a mouse created in 1974 by ... sheep and pigs by microinjection". Nature. 315 (6021): 680-83. Bibcode:1985Natur.315..680H. doi:10.1038/315680a0. PMID 3892305 ...
Zinc finger chimera
July 2009). "Knockout rats via embryo microinjection of zinc-finger nucleases". Science. 325 (5939): 433. Bibcode:2009Sci...325 ...
Zinc finger
"Knockout rats via embryo microinjection of zinc-finger nucleases". Science. 325 (5939): 433. Bibcode:2009Sci...325..433G. doi: ...
Genetic engineering
DNA is generally inserted into animal cells using microinjection, where it can be injected through the cell's nuclear envelope ... "Knockout rats via embryo microinjection of zinc-finger nucleases". Science. 325 (5939): 433. Bibcode:2009Sci...325..433G. doi: ... material followed by the incorporation of that material either indirectly through a vector system or directly through micro- ...
Ralph L. Brinster
... and was at the forefront of the field in applying these microinjection methods to generate transgenic mice. Direct injection of ... sheep and pigs by microinjection". Nature. 315(6021): 680-683. Bibcode:1985Natur.315..680H. doi:10.1038/315680a0. PMID 3892305 ...
Lateral hypothalamus
Consistently, microinjections of orexin-A into the rostral ventrolateral medulla (Huang et al., 2010) or rostral ventromedial ...
Mouse brain
"Genetic transformation of mouse embryos by microinjection of purified DNA". Proceedings of the National Academy of Sciences. 77 ...
Genetically modified crops
... micro-injection and particle bombardment with a gene gun (invented in 1987). In the 1980s techniques were developed to ... Microinjection is used to directly inject foreign DNA into cells. Plant scientists, backed by results of modern comprehensive ... microinjection and agrobacterium. More recently, CRISPR and TALEN offered much more precise and convenient editing techniques. ...
George Scangos
"Genetic transformation of mouse embryos by microinjection of purified DNA". Proceedings of the National Academy of Sciences. 77 ...
Genetically modified mouse
"Genetic transformation of mouse embryos by microinjection of purified DNA". Proc. Natl. Acad. Sci. USA. 77 (12): 7380-7384. ...