... proteins become localized at the kinetochores of all sister-chromatid pairs. Mad2 is capable of forming multimers and ... Binding partners of Mad2 include either Cdc20 or Mad1. Mad1 and Cdc20 bind Mad2 in an identical fashion. Mad2 uses the same ... Cdc20:Mad2 then dissociates and Mad1:Mad2 is enabled to bind a free cytosolic Mad2 again. It is speculated that once formed, ... In this model, external Open Mad2 is recruited to the Mad1:Mad2 template. This Mad1:Mad2 interaction is thought to enable a ...

Tang Z, Bharadwaj R, Li B, Yu H (August 2001). "Mad2-Independent inhibition of APCCdc20 by the mitotic checkpoint protein BubR1 ... Fang G (March 2002). "Checkpoint protein BubR1 acts synergistically with Mad2 to inhibit anaphase-promoting complex". Molecular ... The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/ ... BubR1 protein levels are shown to decline with age. Furthermore, loss of BubR1 in young organisms is associated with rapid ...

"Chromosome missegregation and apoptosis in mice lacking the mitotic checkpoint protein Mad2". Cell. 101 (6): P635-645. doi: ... "Friendster for Proteins". Forbes. Feb 23, 2007. Retrieved 17 December 2021. Spencer, S. L.; Gaudet, S.; Albeck, J. G.; Burke, J ... Sorger, P. K.; Pelham, H. R. (9 September 1988). "Yeast heat shock factor is an essential DNA-binding protein that exhibits ... Recent work has focused on deep learning as means to further understand complex protein networks and drug mechanisms. The ...

Fang G, Yu H, Kirschner MW (1998). "The checkpoint protein MAD2 and the mitotic regulator CDC20 form a ternary complex with the ... Fang G, Yu H, Kirschner MW (June 1998). "The checkpoint protein MAD2 and the mitotic regulator CDC20 form a ternary complex ... The APC/CCdc20 is an important target of the SAC, which consists of several different proteins, including Mad2, Mad3(BubR1), ... CDC20 is a protein related to the beta subunit of heterotrimeric G proteins. Near its C-terminus it contains seven WD40 repeats ...

MAD2), and of the metalloprotease-disintegrin MDC9 with a novel MAD2-related protein, MAD2beta". Biochem. J. 343 (3): 673-80. ... MAD2), and of the metalloprotease-disintegrin MDC9 with a novel MAD2-related protein, MAD2beta". Biochem. J. 343 (3): 673-80. ... Fang G, Yu H, Kirschner MW (1998). "The checkpoint protein MAD2 and the mitotic regulator CDC20 form a ternary complex with the ... Fang G, Yu H, Kirschner MW (June 1998). "The checkpoint protein MAD2 and the mitotic regulator CDC20 form a ternary complex ...

"Role of Hec1 in spindle checkpoint signaling and kinetochore recruitment of Mad1/Mad2". Science. 297 (5590): 2267-70. Bibcode: ... 40S ribosomal protein S27 also known as metallopan-stimulin 1 or MPS-1 is a protein that in humans is encoded by the RPS27 gene ... The protein belongs to the S27E family of ribosomal proteins. It contains a C4-type zinc finger domain that can bind to zinc. ... Studies in rat indicate that ribosomal protein S27 is located near ribosomal protein S18 in the 40S subunit and is covalently ...

"Specific association of estrogen receptor beta with the cell cycle spindle assembly checkpoint protein, MAD2". Proc. Natl. Acad ... The nuclear receptor coactivator 2 also known as NCoA-2 is a protein that in humans is encoded by the NCOA2 gene. NCoA-2 is ... Chen D, Ma H, Hong H, Koh SS, Huang SM, Schurter BT, Aswad DW, Stallcup MR (1999). "Regulation of transcription by a protein ... Naltner A, Ghaffari M, Whitsett JA, Yan C (2000). "Retinoic acid stimulation of the human surfactant protein B promoter is ...

"Specific association of estrogen receptor beta with the cell cycle spindle assembly checkpoint protein, MAD2". Proceedings of ... Thus, ERβ helps control Aβ levels by maintaining the protein it is derived from, β-amyloid precursor protein. ERβ helps by up- ... Upon binding to 17-β-estradiol, estriol or related ligands, the encoded protein forms homo-dimers or hetero-dimers with ... Halachmi S, Marden E, Martin G, MacKay H, Abbondanza C, Brown M (June 1994). "Estrogen receptor-associated proteins: possible ...

"Specific association of estrogen receptor beta with the cell cycle spindle assembly checkpoint protein, MAD2". Proceedings of ... Qi C, Chang J, Zhu Y, Yeldandi AV, Rao SM, Zhu YJ (August 2002). "Identification of protein arginine methyltransferase 2 as a ... Dhamad AE, Zhou Z, Zhou J, Du Y (2016-08-02). Picard D (ed.). "Systematic Proteomic Identification of the Heat Shock Proteins ( ... March 2001). "A subfamily of RNA-binding DEAD-box proteins acts as an estrogen receptor alpha coactivator through the N- ...

"Specific association of estrogen receptor beta with the cell cycle spindle assembly checkpoint protein, MAD2". Proc. Natl. Acad ... "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/ ... "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. Bibcode:2005Natur. ... Méthot N, Rom E, Olsen H, Sonenberg N (1997). "The human homologue of the yeast Prt1 protein is an integral part of the ...

Aurora B may be involved in the localization of MAD2 and BubR1, proteins that recognize correct chromosome attachment to ... 2004). "Identification of the substrates and interaction proteins of aurora kinases from a protein-protein interaction model". ... Aurora B has been shown to bind to end-binding protein 1 (EB1), a protein that regulates microtubule dynamics. Indirect ... including the type-III intermediate filament proteins vimentin, desmin, and glial fibrillary acidic protein (GFAP). In general ...

"Nuf2 and Hec1 are required for retention of the checkpoint proteins Mad1 and Mad2 to kinetochores". Current Biology. 13 (23): ... Kinetochore protein NDC80 homolog is a protein that in humans is encoded by the NDC80 gene. Ndc80 is one of the proteins of ... "Identification of the substrates and interaction proteins of aurora kinases from a protein-protein interaction model". ... This protein complex has microtubule-binding domains. HEC is one of several proteins involved in spindle checkpoint signaling. ...

This protein regulates expression of Mad2 mitotic arrest deficient-like 1, a cell division checkpoint protein. This protein ... PCI domain containing 2 is a protein that in humans is encoded by the PCID2 gene. This gene encodes a component of the TREX-2 ... also interacts with and stabilizes Brca2 (breast cancer 2) protein. Alternative splicing results in multiple transcript ...

2004). "Nuf2 and Hec1 are required for retention of the checkpoint proteins Mad1 and Mad2 to kinetochores". Curr. Biol. 13 (23 ... Kinetochore protein Nuf2 is a protein that in humans is encoded by the NUF2 gene. This gene encodes a protein that is highly ... 2004). "Identification of the substrates and interaction proteins of aurora kinases from a protein-protein interaction model". ... The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a ...

The proteins responsible for the SAC signal compose the mitotic checkpoint complex (MCC), which includes SAC proteins, MAD2/ ... O-Mad2) to the kinetochores. This O-Mad2 changes its conformation to closed Mad2 (C-Mad2) and binds Mad1. This Mad1/C-Mad2 ... O-Mad2) to a closed form (C-Mad2.) The C-Mad2 bound to Mad1 then dimerizes with a second O-Mad2 and catalyzes its closure ... Since MAD1 and CDC20 both contain a similar MAD2-binding motif, the empty O-MAD2 conformation changes to C-MAD2 while binding ...

The complex of proteins which regulate the cell arrest are BUB1, BUB2, BUB3 (this protein), Mad1, Mad2, Mad3 and MPS1. At ... Mitotic checkpoint protein BUB3 is a protein that in humans is encoded by the BUB3 gene. Bub3 is a protein involved with the ... Mad2, Mad3, and the increasingly important Mps1 (a protein kinase). When the SAC is activated, the production of the Bub3-Cdc20 ... Bub3 also acts as a regulator in that it affects binding of Mad3 to Mad2. Structural and sequence analysis indicated the ...

Evidence shows that TRIP13/PCH2 uses p31-Comet as an adaptor protein to convert C-Mad2 into O-Mad2. However, the connection ... open O-Mad2 and closed C-Mad2) (2). When kinetochores are unattached, O-Mad2 converts to C-Mad2, which is then able to latch to ... TRIP13/PCH2 also functions as a kinetochore protein that interacts with the silencing protein p31-Comet. Meiosis in mammalian ... that activation of the SAC and the formation of the MCC requires not only C-Mad2 but also the conversion of C-Mad2 to O-Mad2. ...

... is thought to serve as a platform recruiting other checkpoint and motor proteins as Mad1, Mad2, BubR1, CENP-E and PLK1 to ... At least thirteen other proteins (Mad1, MAD2, MAD3/BubR1, BUB3, Mps1 etc.) are part of the check point, among which many have ... Sharp-Baker H, Chen RH (Jun 2001). "Spindle checkpoint protein Bub1 is required for kinetochore localization of Mad1, Mad2, ... The N-terminal region mediates binding of Hs-BUB1 to the mitotic kinetochore protein blinkin (a protein also commonly referred ...

In molecular biology, the HORMA domain (named after the Hop1p, Rev7p and MAD2 proteins) is a protein domain that has been ... Hop1 is a meiosis-specific protein, Rev7 is required for DNA damage induced mutagenesis, and MAD2 is a spindle checkpoint ... Humans proteins containing this domain include: HORMAD1, HORMAD2, MAD2L1, MAD2L2 He X, Patterson TE, Sazer S (1997). "The ... Eukaryotic Linear Motif resource motif class LIG_MAD2 This article incorporates text from the public domain Pfam and InterPro: ...

Fang G, Yu H, Kirschner MW (June 1998). "The checkpoint protein MAD2 and the mitotic regulator CDC20 form a ternary complex ... coordinate the cellular localisation of proteins; activate and inactivate proteins; and modulate protein-protein interactions. ... coordinate the cellular localisation of proteins; activate and inactivate proteins; and modulate protein-protein interactions. ... Proteins are affected by these modifications in a number of ways: they regulate the degradation of proteins via the proteasome ...

The protein encoded by this gene was identified as a binding protein of the MAD2 mitotic arrest deficient-like 1 (MAD2/MAD2L1 ... Habu T, Kim SH, Weinstein J, Matsumoto T (Dec 2002). "Identification of a MAD2-binding protein, CMT2, and its role in mitosis ... Suzuki H, Fukunishi Y, Kagawa I, Saito R, Oda H, Endo T, Kondo S, Bono H, Okazaki Y, Hayashizaki Y (Oct 2001). "Protein-protein ... MAD2L1-binding protein is a protein that in humans is encoded by the MAD2L1BP gene. ...

The DNA-binding region is capable of doing so independent of the rest of the protein, cleaved or not. It is unable, however, to ... In the absence of TNKs, mitosis arrest is observed in pre-anaphase through Mad2 spindle checkpoint. TNKs can also PARsylate Mcl ... Poly (ADP-ribose) polymerase (PARP) is a family of proteins involved in a number of cellular processes such as DNA repair, ... The auto-modification domain is responsible for releasing the protein from the DNA after catalysis. Also, it plays an integral ...

In the absence of Aurora A mad2, a protein that normally dissipates once a proper kinetochore-microtubule connection is made, ... Among these target proteins are TACC, a microtubule-associated protein that stabilizes centrosomal microtubules and Kinesin 5, ... protein with ch-TOG and GAS41/NuBI1 suggests multiple TACC1-containing protein complexes in human cells". Biochem. J. 363 (Pt 1 ... The MOS protein accumulates until it exceeds a threshold and then transduces the phosphorylation cascade in the map kinase ...

This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved ... Cell division cycle protein 27 homolog is a protein that in humans is encoded by the CDC27 gene. The protein encoded by this ... a protein domain important for protein-protein interaction. ... This protein and 3 other members of the APC complex contain the ... "Mammalian p55CDC mediates association of the spindle checkpoint protein Mad2 with the cyclosome/anaphase-promoting complex, and ...

... kinetochores promote the formation of the mitotic checkpoint complex which is composed of four different proteins known as Mad2 ... This is usually achieved by mutations in the p53 protein or by loss of the chromosome region that contains the genetic code for ... p53 acts to prevent the propagation of tumor cells and is considered a major tumor suppressor protein. p53 works by either ... Tumors cells often have inactivated the machinery that is required for apoptosis such as the p53 protein. ...

In higher eukaryotes, cytoplasmic dynein is involved in silencing the SAC by removing the checkpoint proteins Mad2 and the Rod- ... Centromere/kinetochore protein zw10 homolog is a protein that in humans is encoded by the ZW10 gene. This gene encodes a ... 2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi: ... The encoded protein binds to centromeres during the prophase, metaphase, and early anaphase cell division stages and to ...

"Mammalian p55CDC mediates association of the spindle checkpoint protein Mad2 with the cyclosome/anaphase-promoting complex, and ... This protein and two other APC complex proteins, CDC23 and CDC27, contain a tetratricopeptide repeat (TPR), a protein domain ... This gene encodes a component protein of the APC complex, which is composed of eight proteins and functions as a protein ... Cell division cycle protein 16 homolog is a protein that in humans is encoded by the CDC16 gene. ...

... binds noncovalently to the spindle assembly checkpoint protein MAD2". Proceedings of the National Academy of Sciences of the ... E2 proteins and E3 enzymes are essential for attaching ubiquitin to matrix proteins. Among them, only one type of E1 protein ... Ubiquitin is a protein composed of 76 amino acids. In order for ubiquitin to bind to other proteins, it must go through an ... The amino-terminal rule means that the degradation rate of a protein, that is, the half-life of a protein, varies depending on ...

Through a not-yet-known mechanism, VHL also increases the concentration of MAD2, an important protein of the spindle checkpoint ... The resultant protein is produced in two forms, an 18 kDa and a 30 kDa protein that functions as a tumor suppressor. The main ... The protein encoded by the VHL gene is the substrate recognition component of a protein complex that includes elongin B, ... Li Z, Wang D, Na X, Schoen SR, Messing EM, Wu G (April 2003). "The VHL protein recruits a novel KRAB-A domain protein to ...

Hop/Rev7/Mad2) domain-containing proteins ATG13 and ATG101. ULK1, specifically, appears to be the most essential for autophagy ... December 2000). "Interaction of the Unc-51-like kinase and microtubule-associated protein light chain 3 related proteins in the ... 2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3 (1): 89. ... Ulk1/2 is an important protein in autophagy for mammalian cells, and is homologous to ATG1 in yeast. It is part of the ULK1- ...