Isoelectric focusing
... can resolve proteins that differ in pI value by as little as 0.01. Isoelectric focusing is the first step ... "Multijunction Capillary Isoelectric Focusing Device Combined with Online Membrane-Assisted Buffer Exchanger Enables Isoelectric ... Isoelectric focusing (IEF), also known as electrofocusing, is a technique for separating different molecules by differences in ... Isoelectric focusing is the only step in preparative one-dimensional gel electrophoresis. According to some opinions, living ...
Pier Giorgio Righetti
"Chapter 2 Evolution and development of isoelectric focusing". In Garfin, David; Ahuja, Satinder (eds.). Handbook of isoelectric ... Righetti, P. G.; Drysdale, J. W. (1976). Isoelectric focusing. New York: American Elsevier Pub. Co. 2014, AES Lifetime ... They developed techniques for isoelectric focusing (IEF) using soluble carrier ampholytes and polyacrylamide gels. In 1971, ... Righetti, P. G. (1983). Isoelectric focusing : theory, methodology, and applications. 1st ed. Laboratory techniques in ...
QPNC-PAGE
Isoelectric focusing". Isoelectric focusing. Methods in Enzymology. Vol. 182. pp. 459-77. doi:10.1016/0076-6879(90)82037-3. ... The aim of isoelectric focusing (IEF), for example, is to separate proteins according to their isoelectric point (pI), thus, ... and isoelectric focusing of proteins and nucleic acids, which have applications in both pharmacology and medicine. Proteins ... Under standard conditions, metalloproteins with different molecular mass ranges and isoelectric points have been recovered in ...
Acid dissociation constant
... so different molecules have different isoelectric points. This permits a technique called isoelectric focusing, which is used ... 2005). Handbook of Isoelectric Focusing and Proteomics. Vol. 7. Elsevier. ISBN 0-12-088752-5. Hulanicki, A. (1987). Reactions ... In the case that there is one species of each type, the isoelectric point can be obtained directly from the pK values. Take the ... For substances in solution, the isoelectric point (pI) is defined as the pH at which the sum, weighted by charge value, of ...
Isoelectric point
Isoelectric focusing is also the first step in 2-D gel polyacrylamide gel electrophoresis. In biomolecules, proteins can be ... Henderson-Hasselbalch equation Isoelectric focusing Zeta potential Electrophoretic deposition Isoionic point pK acid ... This is termed the isoelectric point. Thus, the isoelectric point is the value of pH at which the colloidal particle remains ... CRC Press; 1st edition (Hardcover). ISBN 978-1-4200-5188-9 IPC - Isoelectric Point Calculator - calculate protein isoelectric ...
Eastern blot
Reinhart and Malamud; Malamud, D (1982). "Protein transfer from isoelectric focusing gels: the native blot". Analytical ...
Immobilized pH gradient
IPG increased reproducibility of Isoelectric focusing and 2D-Gel Electrophoresis. Other advantages are increased resolution, ... of protein spots in two-dimensional polyacrylamide gel electrophoresis using immobilised pH gradient isoelectric focusing in ... The gel is then washed to remove catalysts and unpolymerized monomers, which interfere with isoelectric separation. ...
BBE-like enzymes
After that, the resulting solution was fractionated using standard procedures and generated, after isoelectric focusing, a ... This value was obtained with isoelectric focusing and chromatofocusing techniques. The enzyme broad temperature range is ... The BBE-like enzyme's optimum pH is 8.9, this means it works in an alkaline medium, but the isoelectric point of the ...
Protein mass spectrometry
The first-dimension of 2D gel is isoelectric focusing (IEF). In this dimension, the protein is separated by its isoelectric ...
Protein
"Fractionation of Complex Protein Mixtures by Liquid-Phase Isoelectric Focusing". 2D PAGE: Sample Preparation and Fractionation ... ISBN 978-0-19-963789-8. Kozlowski LP (January 2017). "Proteome-pI: proteome isoelectric point database". Nucleic Acids Research ... can be monitored using various types of gel electrophoresis if the desired protein's molecular weight and isoelectric point are ...
Shotgun proteomics
Klose J (1975). "Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissues. A novel approach to ... It can be used in projects ranging from large-scale whole proteome to focusing on a single protein family. It can be done in ...
Core-shell semiconductor nanocrystal
"Size-selective separation of gold nanoparticles using isoelectric focusing electrophoresis (IEF)". Chemical Communications (6 ... Clark, Michael D.; Kumar, Sanat K.; Owen, Jonathan S.; Chan, Emory M. (2011-05-11). "Focusing Nanocrystal Size Distributions ...
Zingibain
1995). "Purification of ginger proteases by DEAE-Sepharose and isoelectric focusing". Biochimica et Biophysica Acta (BBA) - ...
Droplet-based microfluidics
"Interface solution isoelectric focusing with in situ MALDI-TOF mass spectrometry". Electrophoresis. 35 (17): 2528-33. doi: ... Flow focusing can also be an active method with the constraint point being adjustable using pneumatic side chambers controlled ... Using specific flow-focusing multiphase designs that control flow rate and temperature, the size of nanoparticle formation can ... Hatch AC, Patel A, Beer NR, Lee AP (April 2013). "Passive droplet sorting using viscoelastic flow focusing". Lab on a Chip. 13 ...
ACES (buffer)
ACES use in isoelectric focusing of proteins has also been documented. Use of ACES has been published in a protocol for the ... Alonso, A., "Human α-1-antitrypsin subtyping by hybrid isoelectric focusing in miniaturized polyacrylamide gel." " ...
Two-dimensional gel electrophoresis
Separation of the proteins by isoelectric point is called isoelectric focusing (IEF). Thereby, a pH gradient is applied to a ... In addition, proteins will not migrate when they have no charge (a result of the isoelectric focusing step) therefore the ... See Isoelectric focusing In quantitative proteomics, these tools primarily analyze bio-markers by quantifying individual ... Klose, J (1975). "Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissues. A novel approach to ...
Tubulin
Williams RC, Shah C, Sackett D (November 1999). "Separation of tubulin isoforms by isoelectric focusing in immobilized pH ... These subunits are slightly acidic, with an isoelectric point between 5.2 and 5.8. Each has a molecular weight of approximately ...
Staphylococcus aureus beta toxin
... comparison of three isoelectric focusing methods". Protein Expression and Purification. 9 (1): 76-82. doi:10.1006/prep. ...
Alpha-1 antitrypsin
As protein electrophoresis is imprecise, the A1AT phenotype is analysed by isoelectric focusing (IEF) in the pH range 4.5-5.5, ... this causes the heterogeneity observed on normal A1AT when analysed by isoelectric focusing. Also, the fucosylated triantennary ... where the protein migrates in a gel according to its isoelectric point or charge in a pH gradient. Normal A1AT is termed M, as ...
Amy Herr
Her thesis is titled "Isoelectric Focusing for Multi-Dimensional Separations in Microfluidic Devices."[citation needed] After ...
Metaproteomics
250, 4007-4021 (1974). Klose, J. Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissues. A novel ...
Amyloidosis
ATTR can be identified using isoelectric focusing which separates mutated forms of transthyretin. Findings can be corroborated ...
Amphoterism
Ampholytes are used to establish a stable pH gradient for use in isoelectric focusing. Metal oxides which react with both acids ... Ate complex Isoelectric point Zwitterion IUPAC, Compendium of Chemical Terminology, 2nd ed. (the "Gold Book") (1997). Online ... The pH at which the average charge is zero is known as the molecule's isoelectric point. ...
Tetrasodium tris(bathophenanthroline disulfonate)ruthenium(II)
"Ultrasensitive fluorescence protein detection in isoelectric focusing gels using a ruthenium metal chelate stain". ...
Bio-MEMS
Isoelectric focusing is the separation of proteins, organelles, and cells with different isoelectric points. Isoelectric ... Examples of continuous-flow separation techniques include continuous-flow electrophoresis, isoelectric focusing, continuous- ... Sorting and focusing of the species of interest is achieved because an electrophoretic force causes perpendicular migration ... Thus, companies focusing on tropical diseases must often combine their research objectives for tropical disease with research ...
ESD (gene)
1987). "Genetic study of red cell esterase D polymorphism by ultrathin layer isoelectric focusing. Distribution in the Veneto ...
SDS-PAGE
Two-dimensional gel electrophoresis sequentially combines isoelectric focusing or BAC-PAGE with a SDS-PAGE. Native PAGE is used ...
Siamese fighting fish
"Genetic variation in different varieties of Siamese fighting fish using isoelectric focusing of sarcoplasmic proteins". ...
Gliadin
... s are prolamins and are separated on the basis of electrophoretic mobility and isoelectric focusing. Gliadin peptides ...
Luschan's salamander
There were around ten molecular forms of esterases present based on isoelectric focusing and gel electrophoresis. The main two ...