Ethyl methanesulfonate
... (EMS) is a mutagenic, teratogenic, and carcinogenic organic compound with formula C3H8SO3. It produces ... "Ethyl Methanesulfonate" (PDF). Report on Carcinogens, Fourteenth Edition. NIEHS. Retrieved 18 June 2021. Sega, Gary A. (1984 ... Ethyl methanesulfonate". Molecular & General Genetics : MGG. 167 (2): 197-207. doi:10.1007/BF00266913. PMID 215891. S2CID ... "A review of the genetic effects of ethyl methanesulfonate". Mutation Research/Reviews in Genetic Toxicology. Elsevier BV. 134 ( ...
Protein engineering
This is because sodium bisulfate catalyses deamination of unmethylated cytosine to uracil.[page needed] Ethyl methane sulfonate ...
ENU
Mutagenesis (molecular biology technique) Ethyl methanesulfonate (EMS) "N-Nitroso-N-ethylurea" (PDF). Report on Carcinogens, ... ENU, also known as N-ethyl-N-nitrosourea (chemical formula C3H7N3O2), is a highly potent mutagen. For a given gene in mice, ENU ... The chemical is an alkylating agent, and acts by transferring the ethyl group of ENU to nucleobases (usually thymine) in ... Salinger, Andrew P.; Justice, Monica J. (2008). "Mouse Mutagenesis Using N-Ethyl-N-Nitrosourea (ENU): Figure 1". Cold Spring ...
Jrk
It can be achieved by utilizing ethyl methanesulfonate (EMS) as a mutagen. The mutation results in a cytosine being swapped for ...
Balancer chromosome
Hermann Muller Invented the Balancer Chromosome Lewis, E. B.; F. Bacher (1968). "Methods of feeding ethyl methane sulphonate ( ... this usually occurs by feeding larvae ethyl methanesulfonate (EMS). The DNA-damaged larvae (or the adults into which they ...
Sodium azide
"Sodium azide mutagenesis in diploid and hexaploid oats and comparison with ethyl methanesulfonate treatments". Environmental ...
Mutagenesis (molecular biology technique)
Ethyl methanesulfonate (EMS) is also often used to generate animal, plant, and virus mutants. In a European Union law (as 2001/ ... In animal studies, alkylating agents such as N-ethyl-N-nitrosourea (ENU) have been used to generate mutant mice. ...
Methanesulfonic acid
... or methanesulfonates, as in ethyl methanesulfonate). It is hygroscopic in its concentrated form. Methanesulfonic acid can ...
Plant breeding
Chemical mutagens like ethyl methanesulfonate (EMS) and dimethyl sulfate (DMS), radiation, and transposons are used for ...
Poekilocerus pictus
Adaptive response of meiotic cells of the grasshopper, Poecilocerus pictus, to a low dose of ethyl methanesulfonate. Mutat Res ...
Agricultural biotechnology
Scientists can use mutating chemicals such as ethyl methanesulfonate, or radioactivity to create random mutations within the ...
Forward genetics
Chemicals like ethyl methanesulfonate (EMS) cause random point mutations particularly in G/C to A/T transitions due to guanine ... Another chemical such as ENU, also known as N-ethyl-N-nitrosourea works similarly to EMS. ENU also induces random point ...
Arabidopsis thaliana
A. thaliana mutants were developed using the mutagen ethyl methanesulfonate and screened to identify mutants with increased ...
Clastogen
Some ligands more than others can stop or reduce the clastogen activity of ethyl methanesulfonate such as adenosine, ADP or DAP ... of clastogen damage seen in chromosomes and reduce the amount of micronuclei affected brought on by ethyl methanesulfonate and ...
Escherichia virus T4
Mutation breeding
Ethyl methanesulfonate (EMS) is the most popular because of its effectiveness and ease of handling, especially its ... There are different kinds of mutagenic breeding such as using chemical mutagens like ethyl methanesulfonate and dimethyl ...
Hugo J. Bellen
These discoveries were made by carrying out multiple forward genetic screens using the mutagen, ethyl methane sulfonate, as ...
Ronald J. Konopka
He used ethyl methanesulfonate (EMS) to induce point mutations in the Drosophila melanogaster genome, and eventually isolated ...
Christiane Nüsslein-Volhard
... generating random mutations in fruit flies using ethyl methanesulfonate. Some of these mutations affected genes involved in the ...
Gerd Jürgens
... ethyl methane sulfonate (EMS)) looking for embryonic defects. His laboratory discovered and characterized the protein KNOLLE, ...
Diepoxybutane
Ethyl methanesulfonate Record in the GESTIS Substance Database of the Institute for Occupational Safety and Health ...
Genetically modified maize
... the herbicide-tolerance trait was bred using tissue culture selection and the chemical mutagen ethyl methanesulfonate, not ...
History of research on Arabidopsis thaliana
George Rédei pioneered the use of A. thaliana for fundamental studies, mutagenizing plants with ethyl methanesulfonate (EMS) ...
EMS
... is the range of all possible electromagnetic radiation Ethyl methanesulfonate (or methanesulfonic acid ethyl ester), a mutagen ...
List of MeSH codes (D02)
... ethyl methanesulfonate MeSH D02.455.326.146.100.050.500.500 - methyl methanesulfonate MeSH D02.455.326.146.100.250 - hepes MeSH ... ethyl methanesulfonate MeSH D02.886.645.600.055.050.510.500 - methyl methanesulfonate MeSH D02.886.645.600.055.050.632 - sodium ... 2-ethyl 2-(4-nitrophenyl) ester MeSH D02.705.539.783 - phorate MeSH D02.705.539.790 - phosmet MeSH D02.705.539.900 - temefos ... 2-ethyl 2-(4-nitrophenyl) ester MeSH D02.886.309.783 - phorate MeSH D02.886.309.790 - phosmet MeSH D02.886.309.900 - temefos ...
IARC group 2B
2-Epoxybutane Ethyl acrylate Ethylbenzene Ethylene dichloride 2-Ethylhexyl acrylate Ethyl methanesulfonate Foreign bodies, ...
Chinese hamster ovary cell
CHO-K1 was mutagenized in the 1970s with ethyl methanesulfonate to generate a cell line lacking dihydrofolate reductase (DHFR) ...
TILLING (molecular biology)
The method combines a standard and efficient technique of mutagenesis using a chemical mutagen such as ethyl methanesulfonate ( ... Draper, BW; McCallum, CM; Stout, JL; Slade, AJ; Moens, CB (2004). "A high-throughput method for identifying N-ethyl-N- ...
Reverse genetics
... a method that combines a standard and efficient technique of mutagenesis with a chemical mutagen such as ethyl methanesulfonate ...
Teff
Sequencing of the teff genome improved breeding, and an ethyl methanesulphonate (EMS)-mutagenized population was then used to ...