Ethidium bromide
... can be degraded chemically, or collected and incinerated. It is common for ethidium bromide waste below a ... Lowe, Derek (2016-04-18). "The Myth of Ethidium Bromide". In the Pipeline. Retrieved 2019-02-28. "Ethidium Bromide: Swap or Not ... ISBN 978-0-470-40753-0. "Application Note: Ethidium Bromide" (PDF). Retrieved 6 April 2014. Borst P (November 2005). "Ethidium ... but it is clear that ethidium bromide can cause mutations in mammalian and bacterial cells. Ethidium bromide is not regulated ...
Ethidium homodimer assay
An ethidium homodimer assay can be used to detect dead or dying cells. Ethidium homodimer is a membrane-impermeable fluorescent ... One reason for doing an ethidium homodimer assay instead of using a TUNEL assay to measure cell death is that ethidium ... Because of this, ethidium homodimer may enter those cells and bind to DNA within those cells. Because live cells don't have a ... After a cell sample has been stained with ethidium homodimer, the dead cells may be viewed and counted under a UV-light ...
Nucleic acid quantitation
Ethidium bromide). This method is useful for cases where concentration is too low to accurately assess with spectrophotometry ...
Electrophoretic color marker
... is ethidium bromide (2,7-diamino-10-ethyl-9-phenylphenanthridiniumbromide).[citation needed] Ethidium Bromide (EtBr) is an ... "Ethidium Bromide - US". www.thermofisher.com. Retrieved 2023-05-11. "SYBR™ Green I Nucleic Acid Gel Stain - 10,000X concentrate ... Nucleic acids are commonly stained and detected using either ethidium bromide or SYBR Green dyes.[citation needed] The most ... common electrophoretic stain in agarose gel is ethidium bromide, however, SYBR green presents greater resolution and yield for ...
Methidiumpropyl-EDTA
Ethidium bromide Tanner, N.Kyle; Cech, Thomas R. (1985). "Self-catalyzed cyclization of the intervening sequence RNA of ...
Polyacrylamide gel electrophoresis
Ethidium bromide binds nucleic acid chains through the process of Intercalation. While Ethidium bromide is a popular stain it ... Ethidium bromide (EtBr) is a popular nucleic acid stain. EtBr allows one to easily visualize DNA or RNA on a gel as EtBr ... Singer VL, Lawlor TE, Yue S (1999). "Comparison of SYBR Green I nucleic acid gel stain mutagenicity and ethidium bromide ... ethidium bromide; or for either, silver stain), allowing visualization of the separated proteins, or processed further (e.g. ...
Base pair
Examples include ethidium bromide and acridine.[citation needed] Mismatched base pairs can be generated by errors of DNA ...
Z-DNA
The toxic effect of ethidium bromide (EtBr) on trypanosomas is caused by shift of their kinetoplastid DNA to Z-form. The shift ... "The killing of African trypanosomes by ethidium bromide". PLOS Pathogens. 6 (12): e1001226. doi:10.1371/journal.ppat.1001226. ...
DNA
Most intercalators are aromatic and planar molecules; examples include ethidium bromide, acridines, daunomycin, and doxorubicin ...
Gel electrophoresis of nucleic acids
Circular DNA are more strongly affected by ethidium bromide concentration than linear DNA if ethidium bromide is present in the ... The ethidium bromide fluoresces reddish-orange in the presence of DNA, since it has intercalated with the DNA. The DNA band can ... Increasing ethidium bromide intercalated into the DNA can change it from a negatively supercoiled molecule into a fully relaxed ... 2000). "Effect of ethidium bromide intercalation on DNA radiosensitivity". Int J Radiat Biol. 76 (1): 1-9. doi:10.1080/ ...
Phenanthridine
Examples of such dyes are ethidium bromide and propidium iodide. It is an isomer of acridine. Phenanthridine was discovered by ...
Fluorescent tag
Ethidium bromide and variants were developed in the 1950s, and in 1994, fluorescent proteins or FPs were introduced. Green ... Ethidium bromide, fluorescein and green fluorescent protein are common tags. The most commonly labelled molecules are ...
Small multidrug resistance protein
Purewal AS (1991). "Nucleotide sequence of the ethidium efflux gene from Escherichia coli". FEMS Microbiol. Lett. 66 (2): 229- ... is Escherichia coli mvrC P23895 which prevents the incorporation of methyl viologen into cells and is involved in ethidium ...
SYBR Safe
However, as the molecule itself is quite a bit larger than ethidium bromide, it does not bind to the column of a gel extraction ... Thiazole Orange has been shown to be three to four times less mutagenic than ethidium bromide whereas SYBR Safe is four to five ... O'Neil, Casey S.; Beach, Jacie L.; Gruber, Todd D. (June 2018). "Thiazole orange as an everyday replacement for ethidium ... SYBR Safe is marketed as a safer alternative to ethidium bromide. ...
Propidium iodide
Viability assay Vital stain SYBR Green I Ethidium bromide "Propidium Iodide". Thermo Fisher Scientific. 2019-11-14. Retrieved ...
Arthur Peacocke
"Circular dichroism in ultraviolet of aminoacridines and ethidium bromide bound to {DNA}". Biopolymers. 10 (10): 1853-1863. doi: ...
Staining
Piet Borst
Molecular-weight size marker
One of the most common dyes used for markers is ethidium bromide. The range of a particular marker refers to variety of bands ...
Propidium monoazide
as an improvement on ethidium monoazide (EMA). PMA provides better discrimination between live and dead bacteria because it is ... Nocker, Andreas; Ching-Ying Cheung, and Anne K. Camper (2006). "Comparison of propidium monoazide with ethidium monoazide for ... Nocker, Andreas; Cheung, Ching-Ying; Camper, Anne K. (2006-11-01). "Comparison of propidium monoazide with ethidium monoazide ... Nocker, Andreas; Cheung, Ching-Ying; Camper, Anne K. (November 2006). "Comparison of propidium monoazide with ethidium ...
Intercalation (biochemistry)
The degree of unwinding varies depending on the intercalator; for example, ethidium cation (the ionic form of ethidium bromide ... Intensively studied DNA intercalators include berberine, ethidium bromide, proflavine, daunomycin, doxorubicin, and thalidomide ... allowing the ethidium to move away from the hydrophilic (aqueous) environment surrounding the DNA and into the intercalation ...
Streptomyces lavendulae
Part V. Reactions of mitomycin C with DNA examined by ethidium fluorescence assay. Canadian Journal of Biochemistry. 54:2 110- ...
Agarose gel electrophoresis
The ethidium bromide may be added to the agarose solution before it gels, or the DNA gel may be stained later after ... Ethidium bromide which intercalates into circular DNA can change the charge, length, as well as the superhelicity of the DNA ... When stained with ethidium bromide, the gel is viewed with an ultraviolet (UV) transilluminator. The UV light excites the ... DNA as well as RNA are normally visualized by staining with ethidium bromide, which intercalates into the major grooves of the ...
Fluorescence in the life sciences
Ethidium bromide's fluorescence is greatly enhanced when it binds to DNA, so this compound is very useful in visualising the ... Ethidium bromide can be toxic - a purportedly safer alternative is the dye SYBR Green. The DNA microarray. Immunology: An ... DNA detection: the compound ethidium bromide, when free to change its conformation in solution, has very little fluorescence. ...
Fluorescence
... the compound ethidium bromide, in aqueous solution, has very little fluorescence, as it is quenched by water. Ethidium ... protected from quenching by water which is excluded from the local environment of the intercalated ethidium. Ethidium bromide ... Intercalated ethidium is in a hydrophobic environment when it is between the base pairs of the DNA, ...
Leptospira biflexa
"Screening of a Leptospira biflexa Mutant Library To Identify Genes Involved in Ethidium Bromide Tolerance". Applied and ...
Viability PCR
Norwegian researchers suggest the use of Ethidium Monoazide, an azide form of Ethidium Bromide, which was used in other ... Nogva, Hege Karin; Drømtorp, Signe Marie; Nissen, Hilde; Rudi, Knut (2003-04-01). "Ethidium monoazide for DNA-based ... Nocker, Andreas; Cheung, Ching-Ying; Camper, Anne K. (2006-11-01). "Comparison of propidium monoazide with ethidium monoazide ...
GelRed
The substance is marketed as a less toxic and more sensitive alternative to ethidium bromide. GelRed is sold as a solution in ... Ethidium bromide GelGreen SYBR Green I Gel electrophoresis Phenanthridine Molecular genetics US application 2010323453, Mao, ... GelRed structurally consists of two ethidium subunits that are bridged by a linear oxygenated spacer. GelRed is a fluorophore, ... and its optical properties are essentially identical to those of ethidium bromide. When exposed to ultraviolet light, it ...
Green chemistry
Ethidium bromide, xylene, mercury, and formaldehyde have been identified as "worst offenders" which have alternatives. Solvents ...
GelGreen
Ethidium bromide GelRed SYBR Green I Agarose gel electrophoresis and gel electrophoresis of nucleic acids Acridine orange US ... The substance is marketed as a less toxic and more sensitive alternative to ethidium bromide. GelGreen is sold as a solution in ...