Gel electrophoresis
... paper electrophoresis 1955 - introduction of starch gels, mediocre separation (Smithies) 1959 - introduction of acrylamide gels ... Gel electrophoresis can also be used for the separation of nanoparticles. Gel electrophoresis uses a gel as an anticonvective ... by native gel electrophoresis, by preparative gel electrophoresis (QPNC-PAGE), or by 2-D electrophoresis. Characterization ... Gel electrophoresis of large DNA or RNA is usually done by agarose gel electrophoresis. See the "Chain termination method" page ...
Null allele
Clayton, J. W.; Tretiak, D. N. (1972-08-01). "Amine-Citrate Buffers for pH Control in Starch Gel Electrophoresis". Journal of ... Many different loci were tested for enzyme activity using a specific gel electrophoresis technique. Alleles that produced an ... Researchers have been able to work around this problem by using detailed Electrophoresis, gel assays, and chromosomal ...
Isozyme
Allozyme Electrophoresis Techniques - a complete guide to starch gel electrophoresis Development of new isozyme specific ... Hunter, R. L.; Merkert, C.L. (1957). "Histochemical demonstration of enzymes separated by zone electrophoresis in starch gels ... Historically, this has usually been done using gels made from potato starch, but acrylamide gels provide better resolution. All ... This assay method requires that the enzymes are still functional after separation (native gel electrophoresis), and provides ...
Zymography
"Improved detection of amylase activity by sodium dodecyl sulfate-polyacrylamide gel electrophoresis with copolymerized starch ... Here a native PAGE gel was run to separate the proteins in a homogenate. Subsequently, a thin gel with starch dissolved (or ... In imprint technique, the enzyme is separated by native gel electrophoresis and the gel is laid on top of a substrate treated ... is embedded in the resolving gel during preparation of the acrylamide gel. Following electrophoresis, the SDS is removed from ...
James E. Bowman
"Characterization of Enterobacteria by Starch-Gel Electrophoresis of Glucose-6-Phosphate Dehydrogenase and Phosphogluconate ...
List of biochemists
British-American geneticist and physical biochemist who introduced starch as a medium for gel electrophoresis. Nobel Prize in ... Charles Cantor (b. 1942). American biophysicist, he developed the method of pulse field gel electrophoresis, and was formerly ... Later she pioneered the use of electrophoresis to study haemoglobin variants. Otto Fritz Meyerhof FRS (foreign member) (1884- ... who developed protein electrophoresis. Nobel Prize for Chemistry (1948). Foreign associate Natl. Acad. Sci. USA. Chen-Lu Tsou ( ...
Evolution of the wolf
Domestic dogs are distinguishable from wolves by starch gel electrophoresis of red blood cell acid phosphatase. During the Last ... Electrophoresis. 25 (14): 2165-2176. doi:10.1002/elps.200305922. PMID 15274000. S2CID 11567406. Birky, C. William (2001). "The ...
Kathleen Margaret Cole
... using starch gel electrophoresis". Thirteenth International Seaweed Symposium. pp. 179-183. doi:10.1007/978-94-009-2049-1_25. ...
Russell's viper
"Identification of enzymes and toxins in venoms of Indian cobra and Russell's viper after starch gel electrophoresis". J. Biol. ... "Fractionation of Vipera russelli venom by gel filtration. I. Venom composition and relative fraction function". Toxicon. 5 (3 ...
Oliver Smithies
He is known for introducing starch as a medium for gel electrophoresis in 1955, and for the discovery, simultaneously with ... Smithies developed the technique of gel electrophoresis using a starch matrix, as a sideline of (unproductive) research into an ... "The Nobel Prize in Physiology or Medicine 2007". Smithies, Oliver (December 1955). "Zone electrophoresis in starch gels: group ... He was assisted technically in his later electrophoresis work by Otto Hiller. He used starch electrophoresis to reveal ...
History of electrophoresis
Starch gel (and later polyacrylamide and other gels) enabled the efficient separation of proteins, making it possible with ... Pulsed-field gel electrophoresis, and Preparative electrophoresis. Electrophoresis History of chromatography History of ... Zone electrophoresis found widespread application in biochemistry after Oliver Smithies introduced starch gel as an ... which used filter paper or gels as supporting media. By the 1960s, increasingly sophisticated gel electrophoresis methods made ...
Phaedon Fessas
1961 Feb 11;1(7172):297-301 IF=47 Demonstration of small components in red cell haemolysates by starch-gel electrophoresis. ... IF 11,8 Two-dimensional paper-agar electrophoresis of haemoglobin. FESSAS P, KARAKLIS A. Clin Chim Acta. 1962 Jan;7:133-8. IF 2 ...
Crown-of-thorns starfish
... planci using starch-gel electrophoresis. The samples were from localities across the Pacific: Ryukyu archipelago (four ...
Leslie D. Gottlieb
Gottlieb using starch gel electrophoresis, determined that indeed C. lingulata had a high degree of genetic similarity to C. ... Using what was then the most modern molecular technique, starch gel electrophoresis, to determine the genetics of populations ... Gottlieb, L.D. (1971). "Gel electrophoresis: New approach to the study of evolution". BioScience. 21 (18): 939-944. doi:10.2307 ... "Assessment of Pleiotropic Effects of a Gene Substitution in Pea by Two-Dimensional Polyacrylamide Gel Electrophoresis". ...
Flat-spired three-toothed snail
Species status was also confirmed in 1988 by starch-gel electrophoresis of foot tissue, while examining evolutionary ...
Harry Harris (geneticist)
The second research focus was on gene product analysis, where he used starch gel electrophoresis to explore the genetic ... Handbook of Enzyme Electrophoresis in Human Genetics (1976) Advances in Human Genetics (1965-1990). A journal that Harris ... Giblett, Eloise R. (January 1977). "Book Review: Handbook of enzyme electrophoresis in human genetics". American Journal of ...
14-3-3 protein
... were located in the 14th fraction eluting from a DEAE-cellulose column and in position 3.3 on a starch electrophoresis gel. 14- ... 14-3-3 proteins were initially found in brain tissue in 1967 and purified using chromatography and gel electrophoresis. In ...
Orchis pallens
... pallens and their hybrids were analysed by enzyme electrophoresis on starch gels, this a form of species identification using ... by Enzyme Electrophoresis". Plant Systematics and Evolution. 153 (3): 229-241. Buttler, Karl Peter (2001). "Taxonomy of ...
QPNC-PAGE
Gordon AH (1969). Electrophoresis of proteins in polyacrylamide and starch gels (Part I, Chapter 2 Acrylamide gel). pp. 34-45. ... Electrophoresis Gel electrophoresis Agarose gel electrophoresis Protein electrophoresis Electroelution Protein purification ... This standardized variant of native gel electrophoresis and subset of preparative polyacrylamide gel electrophoresis is used by ... cite book}}: ,journal= ignored (help) Woolley P (1987). "Thermal instability of electrophoresis gels". Electrophoresis. 8 (8): ...
List of atheists in science and technology
He is known for introducing starch as a medium for gel electrophoresis in 1955 and for the discovery, simultaneously with Mario ...
Pregnancy zone protein
This was a result of an experimental process using starch gel zone-electrophoresis, which detected a protein band in the sera ...
Polydora glycymerica
Researchers used starch gel electrophoresis to compare the number of isozyme loci and the allozymic variation present in both ...
List of geneticists
... created starch gel electrophoresis to separate proteins Bruce Lahn (born 1969), Chinese-born geneticist specializing in ... gel electrophoresis, knockout mice George Snell (1903-1996), US mouse geneticist, pioneer transplant immunologist, won Nobel ...
SDS-PAGE
When using different buffers in the gel (discontinuous gel electrophoresis), the gels are made up to one day prior to ... in contrast to the previously used paper discs or starch gels, provided a higher stability of the gel and no microbial ... For the gel solution, acrylamide is mixed as gel-former (usually 4% V/V in the stacking gel and 10-12 % in the separating gel ... These gels are delivered cast and ready-to-use. Since they use only one buffer (continuous gel electrophoresis) and have a ...
Isoelectric focusing
Proteins are introduced into an Immobilized pH gradient gel composed of polyacrylamide, starch, or agarose where a pH gradient ... Isoelectric focusing is the first step in two-dimensional gel electrophoresis, in which proteins are first separated by their ... Isoelectric focusing is the only step in preparative one-dimensional gel electrophoresis. According to some opinions, living ... Microchip based electrophoresis is a promising alternative to capillary electrophoresis since it has the potential to provide ...
List of MeSH codes (E05)
... electrophoresis, gel, two-dimensional MeSH E05.196.401.485 - electrophoresis, starch gel MeSH E05.196.401.500 - electrophoretic ... gel, pulsed-field MeSH E05.196.401.250 - electrophoresis, gel, two-dimensional MeSH E05.196.401.319 - electrophoresis, paper ... electrophoresis, agar gel MeSH E05.196.401.153.150 - comet assay MeSH E05.196.401.190 - electrophoresis, capillary MeSH E05.196 ... electrophoresis, polyacrylamide gel MeSH E05.196.401.402.236 - electrophoresis, disc MeSH E05.196.401.402.250 - ...
List of MeSH codes (H01)
... electrophoresis, gel, two-dimensional MeSH H01.181.529.307.437.402 - starch gel electrophoresis MeSH H01.181.529.307.437.568 - ... electrophoresis, gel, pulsed-field MeSH H01.181.529.307.437.230 - electrophoresis, gel, two-dimensional MeSH H01.181.529.307. ... electrophoresis MeSH H01.181.529.307.437.100 - agar gel electrophoresis MeSH H01.181.529.307.437.100.150 - comet assay MeSH ... 437.236 - paper electrophoresis MeSH H01.181.529.307.437.319 - polyacrylamide gel electrophoresis MeSH H01.181.529.307.437.319. ...
Eloise Giblett
... began research studying polymorphisms of the human plasma proteins haptoglobin and transferrin using starch gel electrophoresis ...
Biopolymer
Nucleic acid sequence can be determined using gel electrophoresis and capillary electrophoresis. Lastly, mechanical properties ... Starch: Starch is an inexpensive biodegradable biopolymer and copious in supply. Nanofibers and microfibers can be added to the ... Without the fibers, starch has poor mechanical properties due to its sensitivity to moisture. Starch being biodegradable and ... Starch and PLA are commercially available and biodegradable, making them a common choice for packaging. However, their barrier ...
Metaproteomics
The first proteomics experiment was conducted with the invention of two-dimensional polyacrylamide gel electrophoresis (2D-PAGE ... After subjects consumed diets high in resistant starch, it was discovered that several microbial proteins were altered such as ... The current proteomics of microbial community makes use of both gel-based (one-dimensional and two-dimensional) and non-gel ... combined metaproteomics with metagenomics and metabolomics to show the effects of resistant starch on the human intestinal ...