Gel electrophoresis Nonlinear frictiophoresis Kaufmann, Mary Elizabeth (1998). "Pulsed-Field Gel Electrophoresis". Molecular ... Pulsed field gel electrophoresis is a technique used for the separation of large DNA molecules by applying to a gel matrix an ... Pulsed-field gel electrophoresis (PFGE) is a method used to separate large segments of DNA using an alternating and cross field ... Pulsed-Homogeneous Orthogonal Field Gel Electrophoresis (PHOGE) The 90 degree angle orientation method, also known as Pulsed ...
Barlow, Denise P.; Lehrach, Hans (1987). "Genetics by gel electrophoresis: the impact of pulsed field gel electrophoresis on ... Barlow, Denise (1989). "Pulsed-field gel electrophoresis". Genome. 31: 465-466. doi:10.1139/g89-084. Sleutels, F; Barlow, DP ( ... Denise P. Barlow (31 January 1950 - 21 October 2017) was a British geneticist who worked in the field of epigenomics. Barlow ...
This number was determined via pulsed-field gel electrophoresis. Since R. rickettsii needs a moving vector to contract the ...
Pulsed field gel electrophoresis (PFGE) Infectious disease Outbreak Foodborne illness "PulseNet". Centers for Disease Control ... Through the network, cooperating groups can share pulsed field gel electrophoresis (PFGE) results which act as fingerprints to ... "Establishment of a universal size standard strain for use with the PulseNet standardized pulsed-field gel electrophoresis ...
"Genomic variation of Arcobacter skirrowii determined by pulsed-field-gel electrophoresis." Meeting Abstract. Pentimalli D, ...
Schwartz, D C; Cantor, C R (1984). "Separation of yeast chromosome-sized DNAs by pulsed field gradient gel-electrophoresis". ... developed pulse field gel electrophoresis for very large DNA molecules. Cantor's three-volume book Biophysical Chemistry, co- ... 2412 on his paper on pulsed field gradient gel-electrophoresis, 1437 on the launching of the ENCODE project (with about 200 ... "Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis". Cell. 37 (1): 67-75. doi:10.1016/0092- ...
"Localization of CYP2F1 by multipoint linkage analysis and pulsed-field gel electrophoresis". Genomics. 10 (1): 284-6. doi: ...
... and pulse strategy for one-dimensional pulsed-field gel electrophoresis (ODPFGE)". Mol Biotechnol. 4 (1): 25-43. doi:10.1007/ ... zero mean periodic electric field is used in zero-integrated-field electrophoresis (ZIFE), where the field time dependence ... This allows to separate long fragments in agarose gel, nonseparable by standard constant field electrophoresis. The long DNA ... For electrophoresis of colloid particles under a small strength electric field, the force f ( t ) {\displaystyle f(t)} in the ...
Detection by pulsed field gel electrophoresis and evidence for involvement of homologous recombination". Journal of Molecular ...
2006). "Characterization of the Legionella anisa population structure by pulsed-field gel electrophoresis". FEMS Microbiol Lett ...
June 2002). "Intra-species chromosome-length polymorphism in Geotrichum candidum revealed by pulsed field gel electrophoresis ...
Pulsed-field gel electrophoresis, detailed methodology on this specific version of gel electrophoresis Gemmill, R. M.; Coyle- ... the Pulsed-field gel electrophoresis (PFGE) process can begin: alternating voltages are combined with a standard gel ... After Pulsed-field gel electrophoresis, a southern blotting technique is performed and detections of specific fragments using ... "Construction of long-range restriction maps in human DNA using pulsed field gel electrophoresis". Gene Analysis Techniques. 4 ( ...
Pilot Study Using Multilocus Sequence Typing and Pulsed-Field Gel Electrophoresis". J. Clin. Microbiol. 40 (3): 868-76. doi: ... "Multilocus Sequence Typing Compared to Pulsed-Field Gel Electrophoresis for Molecular Typing of Pseudomonas aeruginosa". J. ... "Multilocus Sequence Typing Lacks the Discriminatory Ability of Pulsed-Field Gel Electrophoresis for Typing Salmonella enterica ... molecular typing schemes have been proposed to determine the relatedness of pathogens such as pulsed-field gel electrophoresis ...
Separation of very large DNA fragments requires pulse field gel electrophoresis (PFGE). In field inversion gel electrophoresis ... Electrophoresis techniques used in the assessment of DNA damage include alkaline gel electrophoresis and pulsed field gel ... Voltage is also limited by the fact that it heats the gel and may cause the gel to melt if a gel is run at high voltage for a ... High percentage agarose gels should be run with PFGE or FIGE. Low percentage gels (0.1−0.2%) are fragile and may break. 1% gels ...
Other categories of methods include using microarrays, pulsed-field gel electrophoresis, cytogenetics and paired-end tags. ... Rather than being immobilized within a gel matrix, DNA molecules were held in place by electrostatic interactions on a ...
... electrophoresis Gel electrophoresis of nucleic acids Immunoelectrophoresis Isoelectric focusing Isotachophoresis Pulsed-field ... relatedness of Bordetella species as determined by macrorestriction digests resolved by pulsed-field gel electrophoresis". Int ... which used filter paper or gels as supporting media. By the 1960s, increasingly sophisticated gel electrophoresis methods made ... Gel electrophoresis and related techniques became the basis for a wide range of biochemical methods, such as protein ...
Researchers performed pulse field gel electrophoresis, a similar method described by Barton et al., and determined that the ... Extrachromosomal DNA in the form of two large plasmids was detected by pulsed-field gel electrophoresis. Sequencing of the 16S ...
... tuberculosis strains were typed by pulsed field gel electrophoresis. This has now been superseded by variable numbers of tandem ... "DNA polymorphisms in strains of Mycobacterium tuberculosis analyzed by pulsed-field gel electrophoresis: a tool for ... Mycobacteria growth indicator tubes can contain a gel that emits fluorescent light if mycobacteria are grown. It is ...
... pulsed-field gel electrophoresis and metabolic footprinting". Journal of Clinical Microbiology. 51 (1): JCM.02845-12. doi: ... As the field of microbiology becomes increasingly more centered on microbial community structure, exometabolomics has provided ... Exometabolomics, also known as 'metabolic footprinting', is the study of extracellular metabolites and is a sub-field of ... Exometabolomic techniques have been used in the following fields: Metabolite utilization to annotate function of unknown genes ...
Thus, gel electrophoresis techniques like pulsed-field gel electrophoresis can be used in molecular epidemiology to ... Goering, R. (6 August 2010). "Pulsed field gel electrophoresis: A review of application and interpretation in the molecular ... pulsed-field gel electrophoresis is relied upon heavily in molecular epidemiological studies. Molecular epidemiology allows for ... Field, N. (2014). "Strengthening the Reporting of Molecular Epidemiology for Infectious Diseases (STROME-ID): an extension of ...
Intraspecific characterization of Vibrio tapetis strains by use of pulsed-field gel electrophoresis, ribotyping, and plasmid ...
In prokaryotes, pulsed field gel electrophoresis and complete genome sequencing are the predominant methods of genome size ...
History of electrophoresis Electrophoretic mobility shift assay Gel extraction Isoelectric focusing Pulsed field gel ... Instead high percentage agarose gels should be run with a pulsed field electrophoresis (PFE), or field inversion ... Gel electrophoresis can also be used for the separation of nanoparticles. Gel electrophoresis uses a gel as an anticonvective ... but the resolution of over 6 Mb is possible with pulsed field gel electrophoresis (PFGE). Polyacrylamide gels are run in a ...
... comparison with pulsed-field gel electrophoresis and spa-typing". PLOS ONE. 4 (4): e5082. Bibcode:2009PLoSO...4.5082S. doi: ... "Establishment of a universal size standard strain for use with the PulseNet standardized pulsed-field gel electrophoresis ... uses BioNumerics to compare pulsed field gel electrophoresis (PFGE) patterns and whole genome sequences from different ... organisms or samples under study and are characterized by a unique key and by a number of user-defined information fields. Each ...
He has also developed electroporation techniques, a method for pulsed-field gel electrophoresis, and methods for analyzing ... "Electrophoresis using contour-clamped electric fields". "Significance analysis of microarrays". Gilbert Chu: DNA Dreamer [1] ... electrophoresis, and statistical methods for microarrays. His other notable contributions include discovering and ... "Separation of large DNA molecules by contour-clamped homogeneous electric fields" Science 234 (4783): 1582-1585 Dec 19, 1986 ...
Using pulsed-field gel electrophoresis (PFGE), size of the H. rufescens nucleomorph genome was estimated to be 580 kb. The most ...
... and Pulsed-Field Gel Electrophoresis Profiling". Journal of Clinical Microbiology. 51 (1): 9-14. doi:10.1128/JCM.05124-11. ISSN ...
Pulsed-field gel electrophoresis is a technique used to separate large DNA in an electric field that periodically changes ... be run through a gel electrophoresis to allow diagnostics of the organism by referencing back to previous gel electrophoresis ... Gel electrophoresis is a technique to separate macromolecules by taking advantage of the charge on many of the molecules found ... By cutting segments of the DNA with restriction enzymes, pulse-field can be used to separate out the segments of DNA. ...
... reservoirs of Clostridium difficile during an outbreak associated with North American pulsed-field gel electrophoresis type 1 ...
... and pulsed-field gel electrophoresis". Journal of Clinical Microbiology. 36 (8): 2214-9. doi:10.1128/jcm.36.8.2214-2219.1998. ...