The possibility that BrdU being substituted for thymine in the genomic DNA could induce double stranded chromosomal breaks and ... Strand-seq (single-cell and single-strand sequencing) was one of the first single-cell sequencing protocols described in 2012. ... Single-cell DNA template strand sequencing, or Strand-seq, is a technique for the selective sequencing of a daughter cell's ... Unlike single cell sequencing protocols, Strand-seq does not utilize multiple displacement amplification or MALBAC for DNA ...
"Breaking and joining single-stranded DNA: the HUH endonuclease superfamily". Nature Reviews Microbiology. Nature Portfolio. 11 ... However they do find it to be highly conserved, 98% of successfully infected plants testing positive for Clink DNA. Timchenko ...
The ~400-aa Hel domain is classified as a 5' to 3' DNA Hel which is involved in the breaking and joining of single-stranded DNA ... 2013). "Breaking and Joining Single-stranded DNA: The HUH Endonuclease Superfamily". Nature Reviews Microbiology. 11 (8): 525- ... the target strands do not occur at the same time since a single-stranded circular DNA is first formed with one of the strands ... The free 3' OH in the target DNA attacks the DNA-Y1 bond and forms a bond with the donor strand resulting in strand transfer. ...
Short stretches of single stranded DNA containing such damaged nucleotide are removed from duplex DNA by separate endonucleases ... Nucleases variously effect single and double stranded breaks in their target molecules. In living organisms, they are essential ... During DNA replication, DNA polymerases elongate new strands of DNA against complementary template strands. Most DNA ... These AP sites are removed by AP endonucleases, which effect single strand breaks around the site. Nucleotide excision repair, ...
CSB also accumulates at sites of DNA double-strand breaks in a transcription dependent manner and influences double-strand ... Chiu CF, Tsai MH, Tseng HC, Wang CL, Tsai FJ, Lin CC, Bau DT (2008). "A novel single nucleotide polymorphism in ERCC6 gene is ... Batenburg NL, Thompson EL, Hendrickson EA, Zhu XD (2015). "Cockayne syndrome group B protein regulates DNA double-strand break ... CSB protein facilitates homologous recombinational repair of double-strand breaks and represses non-homologous end joining. In ...
Cells have mechanisms for repairing single-strand DNA damage and double-stranded DNA damage. However, double-stranded DNA ... Targeting double-stranded breaks increases the probability that cells will undergo cell death. Cancer cells are generally less ... Single-strand DNA damage is then passed on through cell division; damage to the cancer cells' DNA accumulates, causing them to ... of tumor oxygen supply because these particles act mostly via direct energy transfer usually causing double-stranded DNA breaks ...
Cell-free extracts have been instrumental to unravel the signaling pathways activated in response to DNA double-strand breaks ( ... "Checkpoint signaling from a single DNA interstrand crosslink". Molecular Cell. 35 (5): 704-715. doi:10.1016/j.molcel.2009.08. ... "Rapid activation of ATM on DNA flanking double-strand breaks". Nature Cell Biology. 9 (11): 1311-1318. doi:10.1038/ncb1651. ... "Checkpoint signaling from a single DNA interstrand crosslink". Molecular Cell. 35 (5): 704-715. doi:10.1016/j.molcel.2009.08. ...
"Entrez Gene: TDP1 tyrosyl-DNA phosphodiesterase 1". El-Khamisy SF, Caldecott KW (2007). "TDP1-dependent DNA single-strand break ... 2005). "Defective DNA single-strand break repair in spinocerebellar ataxia with axonal neuropathy-1". Nature. 434 (7029): 108- ... overhangs of DNA double strand breaks by the human tyrosyl-DNA phosphodiesterase hTdp1". J. Biol. Chem. 277 (30): 27162-8. doi: ... This protein may also remove glycolate from single-stranded DNA containing 3-prime phosphoglycolate, suggesting a role in ...
"Breaking and joining single-stranded DNA: the HUH endonuclease superfamily". Nature Reviews Microbiology. 11 (8): 525-538. doi: ... HUH endonucleases (HUH-tags) are sequence-specific single-stranded DNA (ssDNA) binding proteins originating from numerous ... "Sequence-Directed Covalent Protein-DNA Linkages in a Single Step Using HUH-Tags". Journal of the American Chemical Society. 139 ... and TrwC relaxases used in orthogonal assembly on DNA nanostructures PCV2 Rep protein fused to luciferase linked to DNA ...
The cleavage is symmetrical, and gives two recombined DNA molecules with single stranded breaks. The eukaryotic mechanism is ... Branch migration is the second step of genetic recombination, following the exchange of two single strands of DNA between two ... It can also be seen when a foreign piece of DNA invades the strand. The mechanism for branch migration differs between ... RuvB is a hexamer with helicase activity, and also binds the DNA. As ATP is hydrolyzed, RuvB rotates the recombined strands ...
Due to hydrolytic depurination, DNA fragments into smaller pieces, leading to single-stranded breaks. Combined with the damage ... December 2015). "New insights on single-stranded versus double-stranded DNA library preparation for ancient DNA". BioTechniques ... A single-stranded DNA (ssDNA) library preparation method has sparked great interest among ancient DNA (aDNA) researchers. In ... June 2006). "Crosslinks rather than strand breaks determine access to ancient DNA sequences from frozen sediments". Genetics. ...
Exposure to coumestrol also causes single stranded breaks in hamster DNA, compromising genome stability. Studies have shown ...
thereby bridging units attached to double-stranded DNA and units attached to single-stranded DNA. There are two main DNA-damage ... that binds to the double-stranded TTAGGG region of the telomere and prevents the recognition of double-strand DNA breaks. ... that bind POT1 to single-stranded DNA, which increase its affinity for single-stranded TTAGGG region of telomeric DNA. POT1 ... POT1a inhibits DNA damage repair at the telomere, whereas POT1b regulates the length of telomeric single-stranded DNA. TPP1 ( ...
In particular, single- and double-strand breaks in DNA can be repaired. The DNA damage checkpoint kinase ATM has a major role ... Koppen G, Verschaeve L (2001). "The alkaline single-cell gel electrophoresis/comet assay: a way to study DNA repair in radicle ... Dormancy breaking often involves changes in membranes, initiated by dormancy-breaking signals. This generally occurs only ... The uptake of water by seeds is called imbibition, which leads to the swelling and the breaking of the seed coat. When seeds ...
Sawada, S.; Okada, S. (1970). "Cysteamine, Cystamine, and Single-Strand Breaks of DNA in Cultured Mammalian Cells". Radiation ... Corry, P. M.; Cole, A. (1968). "Radiation-Induced Double-Strand Scission of the DNA of Mammalian Metaphase Chromosomes". ... The nucleic acids that form from binding to DNA are more stable then unbound nucleic acids. Binding of cystamine to ... The affinity of cystamine to DNA plays a role in the toxicity and radioprotecting properties of cystamine.[citation needed] ...
"XRCC1 stimulates human polynucleotide kinase activity at damaged DNA termini and accelerates DNA single-strand break repair". ... "XRCC1 stimulates human polynucleotide kinase activity at damaged DNA termini and accelerates DNA single-strand break repair". ... "The protein kinase CK2 facilitates repair of chromosomal DNA single-strand breaks". Cell. 117 (1): 17-28. doi:10.1016/S0092- ... polymerase-1-dependent DNA double-strand breaks rejoining pathway". J. Mol. Biol. 356 (2): 257-65. doi:10.1016/j.jmb.2005.11. ...
... causes dose-dependent single- and double-stranded breaks in DNA and DNA-protein cross-links. The substance has been ... The cytotoxic effects of teniposide are related to the relative number of double-stranded DNA breaks produced in cells, which ... since it does not intercalate into DNA or bind strongly to DNA. ... are a reflection of the stabilization of a topoisomerase II-DNA ... found to act as an inhibitor of topoisomerase II (an enzyme that aids in DNA unwinding), ...
In this step, the 5' strand of the break is degraded by nucleases to create long 3' single-stranded tails. DSBs that have not ... Ku70/80 DNA-PKcs DNA Ligase IV XRCC4 XLF Artemis DNA polymerase mu DNA polymerase lambda PNKP Aprataxin APLF BRCA1 BRCA2 CYREN ... These microhomologies are often present in single-stranded overhangs on the ends of double-strand breaks. When the overhangs ... is a pathway that repairs double-strand breaks in DNA. It is called "non-homologous" because the break ends are directly ...
At various steps of these recombination processes, heteroduplex DNA (double-stranded DNA consisting of single strands from each ... Meiotic recombination can be initiated by a double-strand break (DSB) in DNA. The 5' ends of the break are degraded, leaving ... During mitosis, the major homologous recombination pathway for repairing DNA double-strand breaks appears to be the SDSA ... When mismatches occur in heteroduplex DNA, the sequence of one strand can be repaired to bind the other strand with perfect ...
Görsdorf S, Appel KE, Engeholm C, Obe G.; Nitrogen dioxide induces DNA single-strand breaks in cultured Chinese hamster cells: ... Although toxic, VHP breaks down in a short time to water and oxygen. Peracetic acid (0.2%) is a recognized sterilant by the FDA ... The four hour cycle uses a single use gas generation ampoule and a disposable scrubber to remove nitrogen dioxide gas. Ozone is ... In hospitals, most EO sterilizers use single-use cartridges because of the convenience and ease of use compared to the former ...
PNKP is active in the repair of DNA damage by the processes of base excision repair, single-strand break repair and non- ... These activities, acting together, prepare single-strand breaks with damaged termini for ligation. Hofmann K, Bucher P ( ... homologous end joining in the case of double strand breaks. In these activities PNKP acts both as a kinase and a phosphatase in ... It spans approximately 80-100 amino acid residues folded into an 11-stranded beta sandwich, which sometimes contains small ...
... single-strand breaks in DNA, double-strand breaks in DNA, the intercalation of foreign substances into the DNA double helix, or ... leading to single-stranded breaks. When the coding strand is single-stranded, it can also hybridize with itself, creating DNA ... For single stranded breaks, replication occurs until the location of the break, then the other strand is nicked to form a ... potentially causing single-stranded DNA breaks. In yeast, proteins act as barriers at the 3' of the transcription unit to ...
... relies on DNA repair pathways to resist DNA damage. Double-strand breaks are especially threatening to ... SSA involves single-strand resection, annealing of the repeats, flap removal, gap filling and ligation. In M. smegmatis the SSA ... SSA is employed as a repair pathway when a double-strand break arises between direct repeat sequences in DNA. ... The NHEJ pathway for repairing double-strand breaks involves the rejoining of the broken ends. It does not depend on a second ...
Both the excision and insertion of the transposon leaves single or double stranded gaps in the DNA, which are repaired by host ... The interaction of these transposases activates the complex; transposase makes double stranded breaks in the DNA and the ... Transposase creates a double stranded break in the DNA and integrates the transposon into the target site. ... Transposase acts like DNA scissors; it is an enzyme that cuts through double-stranded DNA to remove the transposon, then ...
Pardo, B; Gomez-Gonzales, B; Aguilera, A (March 2009). "DNA repair in mammalian cells: DNA double-strand break repair: how to ... At some later point the DNA ends are processed so that unnecessary residuals of chemical groups are removed and single strand ... The polymerase synthesizes the missing part of the broken strand. When the broken strand is rebuilt, both strands need to ... When a double strand DNA lesion is repaired by NHEJ there is no validating DNA template present so it may result in a novel DNA ...
An enzyme system is present in oocytes that ordinarily accurately repairs DNA double-strand breaks. This repair system is ... Primordial follicles are immature primary oocytes surrounded by a single layer of granulosa cells. ... As women age, double-strand breaks accumulate in the DNA of their primordial follicles. ... "Impairment of BRCA1-related DNA double-strand break repair leads to ovarian aging in mice and humans". Science Translational ...
The resulting peroxide decomposes to form single- or double-stranded breaks in DNA, ultimately leading to cell death. With ... producing single-strand breaks. Puzzlingly, while the structure of kedarcidin chromophore is most closely related to that of ... to its DNA target. Thus, the general mechanism by which kedarcidin chromophore damages DNA is known; however, the details of ... including the deoxyribose sugars of DNA. This generates a carbon-centered free radical on DNA, which undergoes oxidation by ...
"Base excision repair causes age-dependent accumulation of single-stranded DNA breaks that contribute to Parkinson's disease ... and the role of DNA in determining interactions between transcription factors and co-factors. His laboratory at IMBB was the ... "Heritable and inducible genetic interference by double-stranded RNA encoded by transgenes". Nature Genetics. 24 (2): 180-3. doi ... "Heritable and inducible genetic interference by double-stranded RNA encoded by transgenes". Nature Genetics. 24 (2): 180-183. ...
Lai, H; Singh, NP (1995). "Acute low-intensity microwave exposure increases DNA single-strand breaks in rat brain cells" (PDF ... radiation considered safe by government standards and found that the DNA in the brain cells of the rats was damaged-or broken ... Lai published research in 1995 that concluded that low-level microwave radiation caused DNA damage in rat brains. Lai earned a ... were looking at the effects of nonionizing microwave radiation the same type of radiation emitted by cell phones on the DNA of ...
They showed that as women age, double-strand breaks accumulate in the DNA of their primordial follicles. Primordial follicles ... are immature primary oocytes surrounded by a single layer of granulosa cells. An enzyme system is present in oocytes that ... "Impairment of BRCA1-related DNA double-strand break repair leads to ovarian aging in mice and humans". Sci Transl Med. 5 (172 ... normally accurately repairs DNA double-strand breaks. This repair system is referred to as homologous recombinational repair, ...