... (msDNA) is a type of extrachromosomal satellite DNA that consists of a single-stranded DNA ... further extension of the DNA strand presents a problem: as DNA synthesis progresses, the bulky RNA strand extending from the 3 ... Hsu MY, Inouye M, Inouye S (December 1990). "Retron for the 67-base multicopy single-stranded DNA from Escherichia coli: a ... Yee T, Furuichi T, Inouye S, Inouye M (August 1984). "Multicopy single-stranded DNA isolated from a gram-negative bacterium, ...
... that binds to single-stranded regions of deoxyribonucleic acid (DNA). Single-stranded DNA is produced during all aspects of DNA ... Binding of single-stranded DNA to the tetramer can occur in different "modes", with SSB occupying different numbers of DNA ... It has a structure of three beta-strands to a single six-stranded beta-sheet to form a protein dimer. DNA-binding protein ... "Crystal structure of the homo-tetrameric DNA binding domain of Escherichia coli single-stranded DNA-binding protein determined ...
Strand-seq (single-cell and single-strand sequencing) was one of the first single-cell sequencing protocols described in 2012. ... Single-cell DNA template strand sequencing, or Strand-seq, is a technique for the selective sequencing of a daughter cell's ... Unlike single cell sequencing protocols, Strand-seq does not utilize multiple displacement amplification or MALBAC for DNA ... By aligning Strand-seq reads from a single cell to the reference genome, the inherited template strands can be determined. If ...
The single stranded DNA (ssDNA) and complementary double stranded DNA (dsDNA) will form a D-loop structure. Another possible ... the strand would undergo a conformational change to form a triple stranded DNA helix. The triple-stranded DNA (H-DNA) was ... Triple-stranded DNA (also known as H-DNA or Triplex-DNA) is a DNA structure in which three oligonucleotides wind around each ... H-DNA formation involves the formation of single-stranded DNA (ssDNA), which is more susceptible to attack by nucleases. ...
This enzyme is not active on DNA containing a T/G mispair or single-stranded DNA. Barrett TE, Schärer OD, Savva R, Brown T, ... Double-stranded uracil-DNA glycosylase (EC 3.2.2.28, Mug, double-strand uracil-DNA glycosylase, Dug, dsUDG, double-stranded DNA ... Sung JS, Mosbaugh DW (August 2000). "Escherichia coli double-strand uracil-DNA glycosylase: involvement in uracil-mediated DNA ... Double-stranded+uracil-DNA+glycosylase at the U.S. National Library of Medicine Medical Subject Headings (MeSH) Portal: Biology ...
The difference in shape between two single-stranded DNA strands with different sequences can cause them to migrate differently ... Single-strand conformation polymorphism (SSCP), or single-strand chain polymorphism, is defined as a conformational difference ... the physical properties of the double strands are almost identical for both alleles. After denaturation, single-stranded DNA ... A single nucleotide change in a particular sequence, as seen in a double-stranded DNA, cannot be distinguished by gel ...
... is single-stranded, and topologically a circle. That is, the DNA single strand extends from one end of the phage particle to ... Wen JD, Gray DM (March 2004). "Ff gene 5 single-stranded DNA-binding protein assembles on nucleotides constrained by a DNA ... Gray CW (July 1989). "Three-dimensional structure of complexes of single-stranded DNA-binding proteins with DNA. IKe and fd ... The single-stranded Ff phage DNA runs down the central core of the phage, and is protected by a cylindrical protein coat built ...
Reineck P, Wienken CJ, Braun D (January 2010). "Thermophoresis of single stranded DNA". Electrophoresis. 31 (2): 279-286. doi: ... A short introduction to thermophoresis, including helpful animated graphics, is at aerosols.wustl.edu Thermophoresis of DNA in ... Thamdrup LH, Larsen NB, Kristensen A (February 2010). "Light-Induced Local Heating for Thermophoretic Manipulation of DNA in ... Furthermore, thermophoresis has been demonstrated as a versatile technique for manipulating single biological macromolecules, ...
Single stranded closed circular DNA. Many begomoviruses have a bipartite genome: this means that the genome is segmented into ... Briddon RW, Stanley J (January 2006). "Subviral agents associated with plant single-stranded DNA viruses". Virology. 344 (1): ... Briddon RW, Patil BL, Bagewadi B, Nawaz-ul-Rehman MS, Fauquet CM (2010). "Distinct evolutionary histories of the DNA-A and DNA- ... of virion strand DNA replication. Component exchange (pseudorecombination) occurs in this genus. The usual mechanism of ...
Reineck P, Wienken CJ, Braun D (2010). "Thermophoresis of single stranded DNA". Electrophoresis. 31 (2): 279-86. doi:10.1002/ ... DNA, RNA, peptides, small molecules, fragments and ions for interactions with high molecular weight complexes, large molecule ... "Thermophoretic melting curves quantify the conformation and stability of RNA and DNA". Nucleic Acids Res. 39 (8): e52. doi: ...
... double-stranded DNA viruses; (II) single-stranded DNA viruses; (III) double-stranded RNA viruses; (IV) positive-sense single- ... negative-sense single-stranded RNA viruses; (VI) positive-sense single-stranded RNA viruses that replicate through a DNA ... double-stranded DNA viruses that replicate through a single-stranded RNA intermediate. The greatest share of bat-associated ... A single bat can host several different kinds of viruses without becoming ill. Bats have also been shown to be more susceptible ...
Partly double-stranded DNA viruses: Hepadnaviridae. These viruses are enveloped. One family of single-stranded DNA viruses ... Both of these taxa are non-enveloped single-stranded DNA viruses. Human-infecting virus families offer rules that may assist ... Positive single-stranded RNA families: three non-enveloped (Astroviridae, Caliciviridae and Picornaviridae) and four enveloped ... Negative single-stranded RNA families: Arenaviridae, Bunyaviridae, Filoviridae, Orthomyxoviridae, ...
The Single-Stranded DNA Phages. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. OCLC 4491528, ISBN 0-87969-122-0 ... Comprehensive Virology: Reproduction of Bacterial DNA Viruses. Plenum Press, New York. OCLC 2331482, ISBN 0-306-35147-1, Google ...
Note that conceptually, DNA single stranded origami is more related to RNA origami than DNA origami. Though RNA nanotechnology ... "Single-stranded DNA and RNA origami". Science. 358 (6369): eaao2648. doi:10.1126/science.aao2648. ISSN 0036-8075. PMC 6384012. ... More recently, RNA origami was extended to the design of long single stranded RNA sequences able to fold into large pre-defined ... TectoRNAs are typically originating from single stranded RNA molecules and once folded, they act like LEGO bricks to build up ...
They have linear, single-stranded DNA (ssDNA) genomes that typically contain two genes encoding for a replication initiator ... Parvoviruses have linear, single-stranded DNA (ssDNA) genomes that are about 4-6 kilobases (kb) in length. The parvovirus ... "Recombination in eukaryotic single stranded DNA viruses". Viruses. 3 (9): 1699-1738. doi:10.3390/v3091699. PMC 3187698. PMID ... Genomic DNA strands in mature virions may be positive-sense or negative-sense. This varies from species to species as some have ...
... double-stranded RNA virus. The genome is segmented. Circoviruses are small single-stranded DNA viruses. There are two genera: ... Parvoviruses are linear, non-segmented single-stranded DNA viruses, with an average genome size of 5000 nucleotides. They are ... Pestiviruses have a single stranded, positive-sense RNA genomes. They cause Classical swine fever (CSF) and Bovine viral ... African swine fever virus (ASFV) is a large double-stranded DNA virus which replicates in the cytoplasm of infected cells and ...
... es (+ssRNA viruses) are a group of related viruses that have positive-sense, single-stranded genomes ... Smith EC, Denison MR (5 December 2013). "Coronaviruses as DNA wannabes: a new model for the regulation of RNA virus replication ... Modrow S, Falke D, Truyen U, Schätzl H (2013). "Viruses with Single-Stranded, Positive-Sense RNA Genomes". Molecular Virology. ... Double-stranded RNA virus Negative-strand RNA virus Sense (molecular biology) "Current ICTV Taxonomy Release , ICTV". ictv. ...
Krupovic M, Ghabrial SA, Jiang D, Varsani A (September 2016). "Genomoviridae: a new family of widespread single-stranded DNA ... Sclerotinia gemycircularvirus 1 is a single stranded DNA virus with a circular genome that infects the fungus Sclerotinia ... Articles with 'species' microformats, Single-stranded DNA viruses). ... The genes are encoded on complementary strands. This virus appears to be related to but distinct from members of the ...
Single-stranded DNA can occur during plasmid replication. Weinberg Z, Lünse CE, Corbino KA, Ames TD, Nelson JW, Roth A, Perkins ... its potential relationship to plasmid replication leaves open the possibility that it functions as a single-stranded DNA. In ... terms of secondary structure, RNA and DNA are difficult to distinguish when only sequence information is available. ...
... es have a circular, single-stranded DNA genome. The genome is negative-sense. Postweaning multisystemic wasting ... DNA loads in serum of healthy and postweaning multisystemic wasting syndrome (PMWS) affected pigs" (PDF). Vet. Microbiol. 152 ( ...
"AID mediates hypermutation by deaminating single stranded DNA". The Journal of Experimental Medicine. 197 (10): 1291-1296. doi: ... Papavasiliou, F. Nina; Schatz, David G. (November 2000). "Cell-cycle-regulated DNA double-strand breaks in somatic ... in DNA, which is recognized as DNA damage and repaired in such a way that introduces thymidine (T), effectively mutating Cs to ... She is best known for her work in the fields of DNA and RNA editing. Papavasiliou received her Bachelors of Science from ...
DNA helicases, DNA clamps and DNA topoisomerases, and replication proteins; e.g. single-stranded DNA binding proteins (SSB). In ... single stranded viruses) or one of the DNA strands (plasmids). The 5′ end of the nicked strand is transferred to a tyrosine ... including DNA gyrase) achieve this by adding negative supercoils to the DNA helix. Bare single-stranded DNA tends to fold back ... each one made up of a single strand of DNA. These two strands serve as the template for the leading and lagging strands, which ...
Bisulfite sequencing only converts single-stranded DNA (ssDNA). Complete bisulfite conversion requires thorough denaturation ... End repair and A-tailing: Due to the nature of how MspI cleaves double stranded DNA, this reaction results in strands with ... Using MspI to digest genomic DNA results in fragments that always start with a C (if the cytosine is methylated) or a T (if a ... End repair is necessary to fill in the 3' terminal of the ends of the strands. The next step is adding an extra adenosine to ...
It is a single stranded DNA virus (ssDNA). There are 49 species in this genus. Some members of this genus cause disease: PCV-1 ... DNA templated transcription, with some alternative splicing mechanism is the method of transcription. The virus exits the host ...
The genome of BBTV is made up of at least six circular, single-stranded DNA components, each about 1 kilo-base pair in length. ... Banana bunchy top is a viral disease caused by a single-stranded DNA virus called the banana bunchy top virus (BBTV). It was ... Banana bunchy top disease affects the banana fruit and foliage, and is caused by a single-strand DNA virus, the banana bunchy ... 1-4. Wang, Xiao-Wei; Blanc, Stéphane (2021-01-07). "Insect Transmission of Plant Single-Stranded DNA Viruses". Annual Review of ...
The encoded protein may bind single-stranded DNA. Model organisms have been used in the study of RTF1 function. A conditional ... De Jong, R. N.; Truffault, V.; Diercks, T.; Ab, E.; Daniels, M. A.; Kaptein, R.; Folkers, G. E. (2008). "Structure and DNA ... DNA Research. 3 (5): 321-329, 341-329. doi:10.1093/dnares/3.5.321. PMID 9039502. v t e (Articles with short description, Short ...
Some include: DNA sequencing; capillary electrophoresis; mass spectrometry; single-strand conformation polymorphism (SSCP); ... Haplotype mapping: sets of alleles or DNA sequences can be clustered so that a single SNP can identify many linked SNPs. ... Single nucleotide substitutions with an allele frequency of less than 1% are sometimes called single-nucleotide variants (SNVs ... An intronic SNP in DNA mismatch repair gene PMS2 (rs1059060, Ser775Asn) is associated with increased sperm DNA damage and risk ...
Formation of the RNA-DNA hybrid would protect the invading single-stranded DNA from degradation. After the transient RNA-DNA ... Thus DNA synthesis fills in gaps left over from annealing, and extends both ends of the still present single stranded DNA break ... ended single DNA strand that in the next step invades a homologous DNA duplex. RNA polymerase III is reported to catalyze ... Kaur H, De Muyt A, Lichten M (February 2015). "Top3-Rmi1 DNA single-strand decatenase is integral to the formation and ...
... and Single-Stranded DNA Polymers". Physical Review Letters. American Physical Society (APS). 92 (4): 048303. Bibcode:2004PhRvL ... Tang, Jau; Marcus, R. A. (2005-11-22). "Single particle versus ensemble average: From power-law intermittency of a single ... "Counting statistics of single molecule reaction events and reaction dynamics of a single molecule". Chemical Physics Letters. ... Colquhoun, D.; Hawkes, A. G. (1982-12-24). "On the Stochastic Properties of Bursts of Single Ion Channel Openings and of ...
Kikuchi M, Ohnishi K, Harayama S (2000-02-08). "An effective family shuffling method using single-stranded DNA". Gene. 243 (1 ... Ogiwara H, Kohno T (2011-12-14). "Essential factors for incompatible DNA end joining at chromosomal DNA double strand breaks in ... First, DNase I is used to fragment a set of parent genes into segments of double stranded DNA ranging from 10-50 bp to more ... In RACHITT, fragments of single stranded (ss) parent genes are annealed onto a ss template resulting in decreased mismatching ...
... sequences that melt become single-stranded and wash off. The temperatures at which labelled DNA comes off reflects the amount ... The mixture is incubated to allow DNA strands to dissociate and then cooled to form renewed hybrid double-stranded DNA. ... "DNA melting". To assess the melting profile of the hybridized DNA, the double-stranded DNA is bound to a column or filter and ... One method was introduced for hybridizing large numbers of DNA samples against large numbers of DNA probes on a single membrane ...