Comet assay
The comet assay is an extremely sensitive DNA damage assay. This sensitivity needs to be handled carefully as it is also ... The single cell gel electrophoresis assay (SCGE, also known as comet assay) is an uncomplicated and sensitive technique for the ... The overall structure resembles a comet (hence "comet assay") with a circular head corresponding to the undamaged DNA that ... Comet) assay. I. Management of invasive transitional cell human bladder carcinoma. II. Fluorescent in situ hybridization Comets ...
Genome instability
Møller, P (2005). "Genotoxicity of environmental agents assessed by the alkaline comet assay". Basic Clin Pharmacol Toxicol. 96 ...
Ingredients of cosmetics
February 2006). "A comet assay study reveals that aluminium induces DNA damage and inhibits the repair of radiation-induced ... Yiu G (December 2004). "Rapid Communications: Antiperspirant Induced DNA Damage in Canine Cells by Comet Assay". Toxicol. Mech ...
Sudan I
Toxicologic Pathology 1982, 10 (2), 71-78 Tsuda, S.; Matsusaka, N.; Madarame, H.; et al., The comet assay in eight mouse organs ... Repeated-dose liver and gastrointestinal tract micronucleus assays with CI Solvent Yellow 14 (Sudan I) using young adult rats. ...
Diana Anderson (scientist)
... "comet assay", which allows for detection of DNA damage with extraordinary sensitivity. Healthy chromosomes do not present Comet ... She worked with Mojgan Najafzadeh on the development of the comet assay test. She worked with IMSTAR to develop an accurate ... Anderson joined the University of Bradford as Chair of Biomedical Research and continued to work on comet assays. She showed ... 2000). "Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing". Environmental and ...
O-Toluidine
"Comparative investigation of multiple organs of mice and rats in the comet assay". Mutat. Res. 517 (1-2): 53-75. doi:10.1016/ ...
Genotoxicity
As a consequence, many sophisticated techniques including Ames Assay, in vitro and in vivo Toxicology Tests, and Comet Assay ... Comet assays are one of the most common tests for genotoxicity. The technique involves lysing cells using detergents and salts ... 2000). "Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing" (PDF). Environmental and ... To assay for genotoxic molecules, researchers assay for DNA damage in cells exposed to the toxic substrates. This DNA damage ...
DNA fragmentation
It can be measured by e.g. the Comet assay or by the TUNEL assay. Its main units of measurement is the DNA Fragmentation Index ... Using bright-field microscopy, the SCD test appears to be more sensitive than the TUNEL assay. DNA Fragmentation plays an ... comparison between sperm chromatin dispersion test and terminal uridine nick-end labeling assay". Fertil. Steril. 94 (3): 1027- ... and TUNEL assay are both effective in detecting sperm DNA damage. ...
Martin Brown (cancer biologist)
"DNA Damage Measured by the Comet Assay in Head and Neck Cancer Patients Treated with Tirapazamine1". Neoplasia.com. Retrieved ...
Helix lucorum
DNA damage in H. lucorum haemocytes and digestive gland cells is determined by the comet assay. Helix lucorum is used in ...
Water immersion objective
Electrophoretic preparations used in the case of comet assay can benefit from the use of water objectives. The refractive index ...
Tryptophol
A Pilot Study with Alkaline Comet Assay". Archives of Industrial Hygiene and Toxicology. 62 (1): 41-49. doi:10.2478/10004-1254- ...
Metolachlor
Comet) assay". Env. Mol. Mut. 29 (3): 277-288. doi:10.1002/(SICI)1098-2280(1997)29:3. 3.0.CO;2-9. PMID 9142171. S2CID 27619855 ...
In vitro fertilisation
Level of DNA fragmentation as measured, e.g. by Comet assay, advanced maternal age and semen quality. Women with ovary-specific ...
Acesulfame potassium
Determination of the effects of some artificial sweeteners on human peripheral lymphocytes using the comet assay. Journal of ...
Platinum nanoparticle
The damage to the DNA was measured for individual cells using single-gel electrophoresis via the comet assay. Researchers have ...
DNA damage theory of aging
Swain, U; Subba Rao, K (Aug 2011). "Study of DNA damage via the comet assay and base excision repair activities in rat brain ... PMID: 25858675; PMCID: PMC4394211 Hashimoto, K; Takasaki, W; Sato, I; Tsuda, S (Aug 2007). "DNA damage measured by comet assay ... Helbock, HJ; Beckman, KB; Shigenaga, MK (January 1998). "DNA oxidation matters: the HPLC-electrochemical detection assay of 8- ... "Gene-specific oxidative lesions in aged rat brain detected by polymerase chain reaction inhibition assay". Free Radic. Res. 41 ...
Tobacco smoking
... in human cells evaluated with the comet assay". Mutat. Res. 629 (1): 7-13. doi:10.1016/j.mrgentox.2006.12.007. PMID 17317274. ...
Smoking
... in human cells evaluated with the comet assay". Mutat. Res. 629 (1): 7-13. doi:10.1016/j.mrgentox.2006.12.007. PMID 17317274. ...
Cigarette
... in human cells evaluated with the comet assay". Mutat. Res. 629 (1): 7-13. doi:10.1016/j.mrgentox.2006.12.007. PMID 17317274. ... Cellulose acetate is degraded within 2-3 weeks under aerobic assay systems of in vitro enrichment cultivation techniques and an ...
Cryptolepine
"Effects of the anti-malarial compound cryptolepine and its analogues in human lymphocytes and sperm in the Comet assay". ...
Lupinus mexicanus
... montanus seeds by using comet assay". Genetics and Molecular Research. 13 (4): 10510-10517. doi:10.4238/2014.December.12.12. ...
Plant senescence
Koppen G, Verschaeve L (2001). "The alkaline single-cell gel electrophoresis/comet assay: a way to study DNA repair in radicle ...
Evolutionary history of plants
Koppen, G.; Verschaeve, L. (2001). "The alkaline single-cell gel electrophoresis/comet assay: a way to study DNA repair in ...
Germination
Koppen G, Verschaeve L (2001). "The alkaline single-cell gel electrophoresis/comet assay: a way to study DNA repair in radicle ...
Seed
Koppen G; Verschaeve L (2001). "The alkaline single-cell gel electrophoresis/comet assay: a way to study DNA repair in radicle ...
Sperm motility
"Sperm DNA damage measured by the alkaline Comet assay as an independent predictor of male infertility and in vitro ...
Edward L. Schneider
N. P. Singh of the "comet" assay for single cell DNA damage. Schneider is the chairperson of the advisory committee to the Los ...
Sperm Chromatin Structure Assay
SCSA has numerous advantages when compared to other sperm DNA fragmentation (sDF) assays [TUNEL assay, COMET assay, and Sperm ... Notably, SCSA outcompetes other convention sperm DNA fragmentation (sDF) assays such as TUNEL and COMET in terms of efficiency ... Sperm Chromatin Structure Assay (SCSA) is a diagnostic approach that detects sperm abnormality with a large extent of DNA ... "Sperm Chromatin Structure Assay (SCSA) , Center for Women's Health , OHSU". www.ohsu.edu. Retrieved 2021-03-31. Evenson, Donald ...
Nicotine
... causes DNA damage in several types of human cells as judged by assays for genotoxicity such as the comet assay, ... Nicotine derived genotoxic effects in human primary parotid gland cells as assessed in vitro by comet assay, cytokinesis-block ...