Gel permeation chromatography
Helmut, D. Gel Chromatography, Gel Filtration, Gel Permeation, Molecular Sieves: A Laboratory Handbook; Springer-Verlag, 1969. ... 2009). Modern size-exclusion liquid chromatography: practice of gel permeation and gel filtration chromatography (2nd ed.). ... Gel permeation chromatography (GPC) is a type of size-exclusion chromatography (SEC), that separates high molecular weight or ... Gel permeation chromatography is conducted almost exclusively in chromatography systems. The experimental design is not much ...
Size-exclusion chromatography
... the technique is known as gel-filtration chromatography, versus the name gel permeation chromatography, which is used when an ... Striegel A, Yau WW, Kirkland JJ, Bly DD (2009). Modern Size-Exclusion Liquid Chromatography: Practice of Gel Permeation and Gel ... While Lathe and Ruthven used starch gels as the matrix, Jerker Porath and Per Flodin later introduced dextran gels; other gels ... Moore JC (1964). "Gel permeation chromatography. I. A new method for molecular weight distribution of high polymers". J Polym ...
Desalting and buffer exchange
Cytiva Animation of desalting using gel filtration chromatography Porath, J; Flodin P (13 June 1959). "Gel Filtration: A method ... These methods are based on gel filtration chromatography, also called molecular sieve chromatography, which is a form of size- ... exclusion chromatography. Desalting and buffer exchange are two of the most common gel filtration chromatography applications, ... There are a number of common formats for performing gel filtration for smaller (less than 4mL) volumes: Chromatography columns ...
Two-dimensional chromatography
Two-dimensional gel electrophoresis Vékey, Károly; Vertes, Akos; Telekes, András (2008). Medical Applications of Mass ... Two-dimensional liquid chromatography (2D-LC) combines two separate analyses of liquid chromatography into one data analysis. ... Gas chromatography-mass spectrometry (GC-MS) is a two-dimensional chromatography technique that combines the separation ... There are two major classifications of 2D liquid chromatography. These include: Comprehensive 2D liquid chromatography (LCxLC) ...
Hinokinin
by silica gel column and counter-current chromatography". Journal of Chromatography B. 929: 6-10. doi:10.1016/j.jchromb.2013.04 ...
Sepharose
Gel permeation chromatography Superose Sephadex Ahmed, Hafiz (2004). Principles and Reactions of Protein Extraction, ...
Thin-layer chromatography
Silica gel is very common in normal-phase TLC. More polar compounds in a sample mixture interact more strongly with the polar ... Thin-layer chromatography (TLC) is a chromatography technique that separates components in non-volatile mixtures. It is ... doi:10.1016/j.chroma.2009.12.071) F. Geiss (1987): Fundamentals of thin layer chromatography planar chromatography, Heidelberg ... Step 1 Step 2 Step 3 Step 4 Step 5 Step 6 Step 7 Radial chromatography HPTLC Column chromatography Harry W. Lewis & Christopher ...
Column chromatography
"Material Harvest Silica Gel for Normal Phase Column Chromatography". Material Harvest. 2008. Retrieved 3 Jan 2019. Fair, JD; ... High performance liquid chromatography (HPLC) for column chromatography using high pressure. Fast protein liquid chromatography ... The most common stationary phase for column chromatography is silica gel, the next most common being alumina. Cellulose powder ... Column chromatography in chemistry is a chromatography method used to isolate a single chemical compound from a mixture. ...
Comet assay
The agarose is gelled at 4 °C and the coverslip removed. The agarose forms a matrix of carbohydrate fibres that encapsulate the ... Journal of Chromatography B. 722 (1-2): 225-254. doi:10.1016/S0378-4347(98)00313-2. PMID 10068143. Singh, N.P.; McCoy, M.T.; ... The single cell gel electrophoresis assay (SCGE, also known as comet assay) is an uncomplicated and sensitive technique for the ... Gedik, C. M.; Ewen, S. W.; Collins, A. R. (September 1992). "Single-cell gel electrophoresis applied to the analysis of UV-C ...
High-performance liquid chromatography
2. Gel filtration chromatography (GFC)-separation of water-soluble biopolymers. GFC uses primarily aqueous solvents (typically ... ligand-exchange chromatography, ion-exchange chromatography of proteins, high-pH anion-exchange chromatography of carbohydrates ... Two types of SEC are usually termed: 1. Gel permeation chromatography (GPC)-separation of synthetic polymers (aqueous or ... Gas chromatography (GC) at the time was more powerful than liquid chromatography (LC), however, it was obvious that gas phase ...
Chiral Lewis acid
The chiral ligand is recovered quantitatively by silica gel chromatography. The chiral (acyloxy) borane (CAB) complex is ...
Polymer Char
... acterization Polyolefin Gel permeation chromatography / Size Exclusion Chromatography Polymer science Polymer ... Molar mass distribution GPC-IR: high temperature gel permeation chromatography (GPC) for polyolefin molar mass distribution. It ... Data Unit 200: signals device for gel permeation chromatography instruments. GPC-QC: simplified and fully automated GPC ... instrument to analyze the polyolefin bivariate distribution by TREF and gel permeation chromatography. SGIC 2D: 3D results with ...
Nucleoside phosphoramidite
... s are purified by column chromatography on silica gel. To warrant the stability of the ...
Bovine serum albumin
"Characterization of proteins and other macromolecules by agarose gel chromatography". Journal of Chromatography A. 152 (1): 21- ...
QPNC-PAGE
Hjertén S (1963). ""Molecular-sieve" electrophoresis in cross-linked polyacrylamide gels". Journal of Chromatography A. 11: 66- ... As a result, the prepared gel is homogeneous (in terms of homogeneous distribution of cross-links throughout the gel sample), ... Gordon AH (1969). Electrophoresis of proteins in polyacrylamide and starch gels (Part I, Chapter 2 Acrylamide gel). pp. 34-45. ... This standardized variant of native gel electrophoresis and subset of preparative polyacrylamide gel electrophoresis is used by ...
Macroprolactin
The gold standard test to diagnose macroprolactin is gel-filtration chromatography. Sadideen H, Swaminathan R. (2006): " ...
Gary Ackers
Thermoresponsive polymers in chromatography
Gewehr, Markus; Nakamura, Katsunori; Ise, Norio; Kitano, Hiromi (1992). "Gel permeation chromatography using porous glass beads ... size exclusion chromatography, ion exchange chromatography, and affinity chromatography separations as well as pseudo-solid ... The research that appeared to spark an onslaught of modified applications was a gel permeation chromatography technique of ... Hydrophobic interaction chromatography requires high concentration salt elutions and eluent cleaning to remove the salt. To ...
Polysuccinimide
... the molar mass of which can be determined by gel permeation chromatography. The process provides reproducible polysuccinimide ...
Silica gel
In chemistry, silica gel is used in chromatography as a stationary phase. In column chromatography, the stationary phase is ... Silica gel is also applied to aluminium, glass, or plastic sheets for thin layer chromatography. The hydroxy (OH) groups on the ... The differences in particle size dictate if the silica gel should be used for flash or gravity chromatography. In this ... Silica gel is also combined with alkali metals to form a M-SG reducing agent. (See SiGNa chemistry) Silica gel is not expected ...
Bachelor of Science in Human Biology
Techniques in experimental biochemistry: Colorimetry, spectrophotometry, spectrofluorometry, pH determination, gel ... electrophoresis, chromatography. THIRD PHASE: The third year was devoted to specialization in a chosen field of anatomy, ...
Gamma-glutamyltransferase
"A high performance gel filtration chromatography method for gamma-glutamyltransferase fraction analysis". Analytical ...
Chromatography in blood processing
... anion exchange chromatography, cation exchange chromatography, and gel filtration chromatography. The recovered purified ... Sepharose fast flow ion exchange and sepharose fast flow chromatography procedures are run, and 3) gel filtration is run. The ... Chromatography is a physical method of separation that distributes the components you want to separate between two phases, one ... There is one big drawback in using chromatography, which has to do with the economics of the process. Although the method was ...
Stokes radius
Stokes radii are often determined experimentally by gel-permeation or gel-filtration chromatography. They are useful in ... Uversky, V.N. (1993). "Use of Fast Protein Size-exclusion Liquid Chromatography to Study the Unfolding of Proteins Which ...
Procyanidin
Gel permeation chromatography (GPC) analysis allows to separate monomers from larger PCO molecules. Monomers of procyanidins ...
Milos Novotny
Keene P. Dimick Award in Chromatography, Third International Symposium on Supercritical Fluid Chromatography Award for ... Novotny was recognized for the development of PAGE Polyacrylamide Gel-filled Capillaries for Capillary Electrophoresis in 1993 ... Tswett Award and Medal in Chromatography; American Chemical Society Award in Chromatography; ISCO Award in Biochemical ... "ACS Award in Chromatography". acs.org. Retrieved November 22, 2019. "ANACHEM". anachem.org. Retrieved November 22, 2019. "LCGC ...
Pepsin
The fragments can be purified by gel filtration, ion exchange, or affinity chromatography. Fab and F(ab')2 antibody fragments ...
Proanthocyanidin
Gel permeation chromatography (GPC) analysis allows separation of monomers from larger proanthocyanidin molecules. Monomers of ... galloyl glucoses and ellagitannins fit on a single calibration curve in high performance-gel permeation chromatography". ... Journal of Chromatography A. 1218 (43): 7804-7812. doi:10.1016/j.chroma.2011.08.082. PMID 21930278. Engström, M. T.; Pälijärvi ...
Coicenal
The yielded dry matter is then purified via gel permeation chromatography, followed by high-performance liquid chromatography. ... After rotary evaporation, this crude extract is purified via silica gel column chromatography. ... They used high-performance liquid chromatography with diode array detection and searched for characteristic UV maximal ...
Keratinase
1990) with the help of membrane ultra filtration and C-75 gel chromatography. He purified enzyme with 70-fold increased ... which shows a 7.5-fold increases in its activity after DEAE cellulose column chromatography. The enzyme-activity was inhibited ...