NHS-coupling gives biotinylation of any primary amines in the protein). Enzymatic biotinylation results in biotinylation of a ... Chemical biotinylation utilises various conjugation chemistries to yield nonspecific biotinylation of amines, carboxylates, ... In contrast to chemical biotinylation methods, enzymatic biotinylation allows biotin to be linked at exactly one residue ... Photoactivatable biotinylation reagents can also be used to activate biotinylation at specific times in an experiment or during ...

Biotinylation basically creates a strong affinity between the biotin on the cytokine-specific antibody and the streptavidin on ... "Biotinylation". ThermoFisher Scientific. Retrieved 6 December 2018. "BCIP/NBT-plus SDS Summary" (PDF). MABTECH. Retrieved 6 ...

Fouda AE, Pflum MK (August 2015). "A Cell-Permeable ATP Analogue for Kinase-Catalyzed Biotinylation". Angewandte Chemie. 54 (33 ... Senevirathne C, Embogama DM, Anthony TA, Fouda AE, Pflum MK (January 2016). "The generality of kinase-catalyzed biotinylation ...

Citrulline residues can be chemically modified with butanedione or by biotinylation prior to analysis, leading to a different ... Tutturen, Astrid E. V.; Holm, Anders; Fleckenstein, Burkhard (2013-11-01). "Specific biotinylation and sensitive enrichment of ...

"Biotinylation by antibody recognition - A method for proximity labeling". Nature Methods. 15 (2): 127-133. doi:10.1038/nmeth. ... "β-Actin mRNA interactome mapping by proximity biotinylation". Proceedings of the National Academy of Sciences. 116 (26): 12863- ...

Biotinylation Siegel L, Foote JL, Coon MJ (March 1965). "The enzymatic synthesis of propionyl coenzyme A holocarboxylase from d ...

... can act as an ATP sensor protein through biotinylation. Biotinylation will immobilize luciferase on the cell-surface ...

2004). "Use of protein biotinylation in vivo for chromatin immunoprecipitation". Analytical Biochemistry. 325 (1): 68-76. doi: ... or in vivo biotinylation can be used instead of antibodies to the native protein of interest. The DNA associated with the ...

Miller, B. T.; Collins, T. J.; Rogers, M. E.; Kurosky, A. (1997). "Peptide biotinylation with amine-reactive esters: ...

Chapman-Smith A, Cronan JE (September 1999). "The enzymatic biotinylation of proteins: a post-translational modification of ...

Chapman-Smith A, Cronan JE (1999). "The enzymatic biotinylation of proteins: a post-translational modification of exceptional ...

ISBN 978-1-118-91840-1. Chapman-Smith A, Cronan JE (September 1999). "The enzymatic biotinylation of proteins: a post- ...

Jander, G; Cronan, J E; Beckwith, J (1996). "Biotinylation in vivo as a sensitive indicator of protein secretion and membrane ...

Protein biotinylation in vivo was proposed to alleviate the problems caused by frequent incompatibility of antibody staining ... Viens, A.; Harper, F.; Pichard, E.; Comisso, M.; Pierron, G.; Ogryzko, V. (2008). "Use of Protein Biotinylation in Vivo for ...

The biotinylation motif Met-Lys-Met is located at the tip of the β-hairpin structure. Rotations around the CαCβ bond of this ... Removal of the thumb by mutagenesis rendered BCCP-87 a favorable substrate for lipoylation but abolished biotinylation (Reche ...

Streptavidin Biotinylation Helppolainen SH, Nurminen KP, Määttä JA, Halling KK, Slotte JP, Huhtala T, et al. (August 2007). " ...

Biotinylation of histone proteins in nuclear chromatin plays a role in chromatin stability and gene expression. The US National ... Biotinylation of histone proteins in nuclear chromatin is a posttranslational modification that plays a role in chromatin ... Biotin deficiency Biotin sulfoxide Biotinidase deficiency Biotinylation Multiple carboxylase deficiency NeutrAvidin Photobiotin ... the chemically modified biotin reagents are bound to the targeted compounds in a solution via a process called biotinylation. ...

Immunofluorescence Biomolecular engineering Biotinylation SpyTag/SpyCatcher Unnatural amino acids Bioconjugate Chemistry ...

The advent of proximity biotinylation by birA* has facilitated the first proteomics-based studies of the cadherin adhesome. ...

... biotinylation or glucose decomposition. Typically, the primary building blocks of these nanostructures are individual ...

biotinylation: covalent attachment of a biotin moiety using a biotinylation reagent, typically for the purpose of labeling a ...

Subtiligase biotinylation of N-termini uses enzymatic labeling of N-terminal peptides, but does not use lysine blocking ... Lysine guanidination followed by biotinylation of N-termini uses a chemical to block lysine residues and tag free N-termini. ... Acetylation of amines followed by tryptic digestion and biotinylation of free N-terminal peptides uses chemical (acetylation) ...

"Identification of new accessible tumor antigens in human colon cancer by ex vivo protein biotinylation and comparative mass ...

In 2009, one idea has been to attach the exonuclease to the nanopore, perhaps through biotinylation to the beta barrel ...

Limitations For Chem-seq to be feasible, the small molecule under study must be amenable to biotinylation without disruption of ...

Biotinylation of DNA and RNA with photoactivatable biotin is easier and less expensive than enzymatic methods since the DNA and ...

The dye nucleotide to be used will likely occur by treatment with a phosphorylation enzyme and biotinylation and reaction of ...

... thereby increasing the abundance of histone H4 biotinylation marks in HEK-293 human kidney cells". The Journal of Nutritional ...

The tag is recognized by a repertoire of single-domain antibodies AviTag, a peptide allowing biotinylation by the enzyme BirA ... such as biotinylation by biotin ligase) or chemical modification (such as coupling to other proteins through SpyCatcher or ...

... biotinylation MeSH E05.393.525.100 - blotting, northern MeSH E05.393.525.150 - blotting, southern MeSH E05.393.525.225 - ...