Localization of nectin-free afadin at the leading edge and its involvement in directional cell movement induced by platelet...
We first examined the effect of afadin on cell movement in response to PDGF stimulation using wild-type and afadin-knockdown NIH3T3 cells. The expression level of afadin in both cell types is shown in Fig. 1A. Wild-type and afadin-knockdown NIH3T3 cells were sparsely plated on μ-Slide VI Flow dishes pre-coated with vitronectin, an extracellular matrix protein that binds to αvβ3 integrin (Schvartz et al., 1999), and were directionally stimulated with PDGF. Wild-type NIH3T3 cells became polarized with the well-spreading leading edge toward the higher concentration of PDGF, whereas afadin-knockdown NIH3T3 cells showed elongated shapes and had a small leading edge that was randomly directed and was independent of the direction of the higher concentration of PDGF (Fig. 1B).. These results led us to assume that afadin is involved in directional cell movement. To ...http://jcs.biologists.org/content/122/23/4319
FRNK blocks v‐Src‐stimulated invasion and experimental metastases without effects on cell motility or growth | The EMBO Journal
Since MMP activity is also regulated by TIMP binding (Nagase and Woessner, 1999) and the dissociation of TIMP-MMP complexes during gel electrophoresis prior to zymography assays acts to enhance the apparent activity of proMMP isoforms, soluble gelatinase activity in the cell‐conditioned media samples was assayed using a peptide substrate (Figure 6C). Both v‐Src3T3 and v‐Src FRNK S‐1034 cells contained high levels of soluble gelatinase activity, whereas NIH‐3T3 and the various v‐Src FRNK cell clones had ∼4‐fold lower levels of gelatinase activity secreted from the same number of cells (Figure 6C). Analysis of whole‐cell lysates also revealed that FRNK expression resulted in lower levels of cell‐associated gelatinase activity (Figure 6D). Although blotting analyses did not reveal significant changes in TIMP expression (data not shown), addition of recombinant TIMP‐2 to v‐Src3T3s inhibited Matrigel invasion ...http://emboj.embopress.org/content/21/23/6289
Localization of nectin-free afadin at the leading edge and its involvement in directional cell movement induced by platelet...
Marian Blanca Ramírez from the CSIC in Spain has been studying the effects of LRRK2, a protein associated with Parkinson's disease, on cell motility. A Travelling Fellowship from Journal of Cell Science allowed her to spend time in Prof Maddy Parson's lab at King's College London, learning new cell migration assays and analysing fibroblasts cultured from individuals with Parkinson's. Read more on her story here. Where could your research take you? The deadline to apply for the current round of Travelling Fellowships is 23rd Feburary 2018. Apply now!. ...http://jcs.biologists.org/content/early/2009/11/03/jcs.048439
British Library EThOS: An investigation into the role of protein kinases in T lymphocyte migration
The migration of T lymphocytes is a vital component of the immune system, with roles in immunosurveillance and inflammation. The role of Phosphoinositide 3-kinase within T lymphocyte migration is unclear, with some evidence that it may be a disposable signal. Here, using Staphylococcal Enterotoxin B activated peripheral blood mononuclear cells and the T cell line CEM cells, the role of Phosphoinositide 3-kinase and its downstream kinases was investigated. CCL22 mediated CEM cell migration and CXCL12 mediated peripheral blood mononuclear cell migration were shown to be independent of Phosphoinositide 3-kinase using several different broad-spectrum Phosphoinositide 3-kinase inhibitors. However, these cells were Akt-dependent, as demonstrated by incubation with the Akt ...http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.501628
"Molecular Mechanism of Tetraspanin CD9 Mediated Cell Motility" by Jayaprakash Kotha
CD9, a member of the tetraspanin superfamily of proteins participates in the regulation of cell adhesive functions such as cell migration. The mechanisms underlying CD9 mediated cell migration are not known. In the current study, we investigated the molecular basis for the CD9 promoted cell migration. Our findings show that the phosphatidylinositol-3 kinase (PI-3K) inhibitors, wortamannin and LY294002 inhibited CD9 promoted cell motility in Chinese hamster ovary (CHO) cells. In contrast, inhibitors targeting protein kinase C or mitogen-activated protein kinase had no effect on CD9 driven CHO cell motility. Furthermore, inhibition of PI-3K activity in CHO cells by dominant/negative PI-3K cDNA transfection abolished CD9 mediated pro-migratory effects. Consistent with these ...http://dc.uthsc.edu/dissertations/130/
Metalloproteinase-Dependent and -Independent processes contribute to inhibition of breast cancer cell migration, angiogenesis...
© 2014 UICC. The ADAMTS proteinases are a family of secreted, matrix-Associated enzymes that have diverse roles in the regulation of tissue organization and vascular homeostasis. Several of the 19 human family members have been identified as having either tumor promoting or suppressing roles. We previously demonstrated that decreased ADAMTS15 expression correlated with a worse clinical outcome in mammary carcinoma (e.g., Porter et al., Int J Cancer 2006;118:1241-7). We have explored the effects of A Disintegrin and Metalloproteinase with Thrombospondin motifs-15 (ADAMTS-15) on the behavior of MDA-MB-231 and MCF-7 breast cancer cells by stable expression of either a wild-type (wt) or metalloproteinase-inactive (E362A) protein. No effects on mammary cancer cell proliferation or apoptosis were observed for either form of ADAMTS-15. However, both forms reduced cell migration on fibronectin or laminin matrices, though motility on ...https://oacentre.kennedy.ox.ac.uk/publications/501422
Metalloproteinase-dependent and -independent processes contribute to inhibition of breast cancer cell migration, angiogenesis...
The ADAMTS proteinases are a family of secreted, matrix-associated enzymes that have diverse roles in the regulation of tissue organization and vascular homeostasis. Several of the 19 human family members have been identified as having either tumor promoting or suppressing roles. We previously demonstrated that decreased ADAMTS15 expression correlated with a worse clinical outcome in mammary carcinoma (e.g., Porter et al., Int J Cancer 2006;118:1241-7). We have explored the effects of A Disintegrin and Metalloproteinase with Thrombospondin motifs-15 (ADAMTS-15) on the behavior of MDA-MB-231 and MCF-7 breast cancer cells by stable expression of either a wild-type (wt) or metalloproteinase-inactive (E362A) protein. No effects on mammary cancer cell proliferation or apoptosis were observed for either form of ADAMTS-15. However, both forms reduced cell migration on fibronectin or laminin matrices, though motility on a Type I ...https://www.kennedy.ox.ac.uk/publications/479671
Internal forces directing cell migration are revealed by live-cell microscopy
How do cells move in a certain direction in the body-go to a wound site and repair it, for example, or hunt down infectious bacteria and kill it?https://phys.org/news/2017-12-internal-cell-migration-revealed-live-cell.html
Cell migration is known to be related to not only physiological phenomena such as embryonic development, immune reaction, and wound healing, but also pathological phenomena such as asthma, vascular disease, and cancer metastasis. However, because genetic mutations of each cancer cell causing high migration ability depend on cell types, it still remains unclear that which pathways are the unity of cancer cell migration signaling irrespective of cancer cell types, and which pathways are the diversity depend on cell types. The aim of this study is to reveal the diversity and unity of regulatory signaling for cancer cell migration based on chemical genomic approach.. To understand the diversity and unity of regulatory signaling for cancer cell ...http://mct.aacrjournals.org/content/10/11_Supplement/C97
ERK2 drives tumour cell migration in 3D microenvironments by suppressing expression of Rab17 and Liprin-β2 | Journal of Cell...
Upregulation of the extracellular signal-regulated kinase (ERK) pathway has been shown to contribute to tumour invasion and progression. Since the two predominant ERK isoforms (ERK1 and ERK2) are highly homologous and have indistinguishable kinase activities in vitro, both enzymes were believed to be redundant and interchangeable. To challenge this view, here we show that ERK2 silencing inhibits invasive migration of MDA-MB-231 cells, and re-expression of ERK2 but not ERK1 restores the normal invasive phenotype. A detailed quantitative analysis of cell movement on 3D matrices indicates that ERK2 knockdown impairs cellular motility by decreasing the migration velocity as well as increasing the time that cells spend not moving. We used gene expression arrays to identify rab17 and liprin-β2 as genes whose expression was increased by knockdown ...http://jcs.biologists.org/content/early/2012/02/08/jcs.092916
Integrin αMβ2-Mediated Cell Migration to Fibrinogen and Its Recognition Peptides | JEM
Leukocyte migration is the hallmark of inflammation in vivo, and αMβ2 and Fg have been shown to contribute to leukocyte migration in multiple systems (23)(24). This study has used αMβ2 transfectants and selected mutants to dissect the molecular requirements for αMβ2-mediated cell migration to Fg and its derivatives. The major conclusions of our study are the following. (a) Fg supports a chemotactic cell migration mediated by αMβ2. This response is dependent on Fg concentration and occurs at low (1-50 μg/ml) Fg levels. (b) The αM I domain is necessary but not sufficient to support cell migration to Fg. In contrast to cell adhesion to Fg, efficient migration requires the β2 subunit. (c) The P1 and P2 peptides, as well as the D100 fragment, ...http://jem.rupress.org/content/193/10/1123
Short courses (certificate) - Introduction to Migration Studies - 1 month - Maastricht University
Would you like to study Behaviour & Social Culture or Business and Economics? All information about Introduction to Migration Studies in Maastricht: admission requirements, deadlines and grants.http://www.eurogates.nl/programs/short-course/introduction-migration-studies/maastricht-university/20115/
Effect of TGFβ1 on the phenotype, migratory ability, a | Open-i
Effect of TGFβ1 on the phenotype, migratory ability, and survival of CD16− monocytes. PBMCs were depleted of CD16+ cells using miniMACS magnetic selection. Thttps://openi.nlm.nih.gov/detailedresult.php?img=PMC2196052_20011608f8&req=4
Natalie Reid | COX-2 contributes to the maintenance of flow-induced dilation | Page 2
All-and genes, ATRA activates a RAR-dependent epithelial differentiation program. pro-migratory determinant to an anti-migratory mediator. Inhibition of the Level1 path not really just takes on a part in the anti-migratory actions of ATRA; it is usually relevant also for the … Continue reading →. ...http://lifescienceexec.com/author/administrator/page/2/
Examples of collective cell migration. First column: sc | Open-i
Examples of collective cell migration. First column: schematic representation of different migratory types. The regions where cells are interacting are depictedhttps://openi.nlm.nih.gov/detailedresult.php?img=PMC2927909_gr2&req=4
mTOR Signaling in Cancer Cell Motility and Tumor Metastasis - Critical Reviews™ in Eukaryotic Gene Expression, Том 20, 2010,...
Tumor cell migration is a key step in the formation of cancer metastasis. The mammalian target of rapamycin (mTOR), a highly conserved and ubiquitously expressed serinethreonine kinase, has been intensely studied for over a decade as a central regulator of cell growth, proliferation, differentiation, and survival. Recent data have shown that mTOR also plays a critical role in the regulation of tumor cell motility and cancer metastasis. Here, we briefly review recent advances regarding mTOR signaling in tumor cell motility. We also discuss recent findings about the mechanism by which rapamycin, a specific inhibitor of mTOR, inhibits cell motility in vitro and metastasis in vivo.. ...http://www.dl.begellhouse.com/ru/journals/6dbf508d3b17c437,275393334da16faf,2e91decb065f0749.html
Protocols and Video Articles Authored by Gersende Alphonse
Gersende Alphonse is the author of these articles in the Journal of Visualized Experiments: 'Isolation and Characterization of a Head and Neck Squamous Cell Carcinoma Subpopulation Having Stem Cell Characteristics', 'Evaluation of the Cell Invasion and Migration Process: A Comparison of the Video Microscope-based Scratch Wound Assay and the Boyden Chamber Assay'https://www.jove.com/author/Gersende_Alphonse
"Cell movement is guided by the rigidity of the substrate" by Chun-Min Lo, Hong-Bei Wang et al.
Directional cell locomotion is critical in many physiological processes, including morphogenesis, the immune response, and wound healing. It is well known that in these processes cell movements can be guided by gradients of various chemical signals. In this study, we demonstrate that cell movement can also be guided by purely physical interactions at the cell-substrate interface. We cultured National Institutes of Health 3T3 fibroblasts on flexible polyacrylamide sheets coated with type I collagen. A transition in rigidity was introduced in the central region of the sheet by a discontinuity in the concentration of the bis-acrylamide cross-linker. Cells approaching the transition region from the soft side could easily migrate across the boundary, with a concurrent increase in spreading area and traction forces. In contrast, cells migrating from the stiff side turned around or retracted as they reached the ...https://escholarship.umassmed.edu/oapubs/269/
2017 Space Migration Planning Project underway
By Cindy Bontrager. The 2017 space migration project is underway to reallocate spaces vacated as a result of the 2016 space migration project. The space migration process allows the university to make strategic, transparent space changes that provide long-term benefits to campus. This phase includes space in Anderson, Fairchild, Holton, Leasure and Seaton halls, as well as Hale Library and Unger Complex - the former old Foundation building. Additional information regarding the available space as well as a schedule of building tours and proposal guidelines is available on the space migration website.. The process for submitting and reviewing space migration proposals will follow a similar format as the previous projects. Concept proposals must be approved and submitted by a dean or vice president by Dec. 16. Final space ...http://www.k-state.edu/today/announcement.php?id=30593
Abi1 is a Novel Target of α4β1 Integrin Signaling - Pendergast Lab
Dynamic signals linking the actin cytoskeleton and cell adhesion receptors are essential for morphogenesis during development and normal tissue homeostasis. Abi1 is a central regulator of actin polymerization and a binding partner of the Abl kinases. The α4 integrin receptor is associated with enhanced protrusive activity and regulation of directional cell migration. Among integrin subunits, α4 predominantly accumulates at the leading edge of migrating cells. We identified Abi1 as a crucial linker between α4 and the actin nucleation machinery at the leading edge. We generated Abi1 knockout mice and found that loss of Abi1 phenocopies knockout of α4. Mice lacking Abi1 or α4 exhibit mid-gestational lethality with abnormalities in placental and cardiovascular development. Abi1 null mice have reduced angiogenesis in the yolk sac, edema, and hemorrhage. Additionally, allantoic explants derived from Abi1 mutant mice exhibit ...https://sites.duke.edu/pendergastlab/2017/03/14/abi1-is-a-novel-target-of-%CE%B14%CE%B21-integrin-signaling/
UNM Cancer Center Scientists Discover Novel Chemical That Controls Cell Behavior - Redorbit
First-in-class chemical compound might control metastases. It´s the spread of the original cancer tumor that kills most people. That´s why cancer researchers vigorously search for drugs that can prevent metastases, the spread of cancer. The research team co-led by Angela Wandinger-Ness, PhD, and Larry Sklar, PhD, at the University of New Mexico Cancer Center has found a chemical compound that appears to control cell migration and adhesion, two important characteristics of metastatic cancer cells. The team recently published a paper describing how the first-in-class compound acts on various cells.. Dr. Wandinger-Ness, a UNM Professor of Pathology and Director of the Fluorescence Microscopy and Cell Imaging Shared Resource, studies proteins called GTPases. GTPases act like chemical switches to control how cells behave: how much a cell grows, what shape it assumes, when it ...http://www.redorbit.com/news/science/1112803743/unm-cancer-center-scientists-discover-novel-chemical-that-controls-cell-behavior/
Come and Find Me | Science Signaling
The paucity of detectable apoptotic cells in tissues where many cells undergo apoptosis demonstrates the efficiency of cell-clearance mechanisms. Apoptotic cells are thought to release factors that signal their presence to scavenger cells such as macrophages, but the nature of these signals is unclear (see commentary by Gregory). Elliott et al. found that supernatant from thymocytes in which apoptosis was induced recruited more monocytes in a migration assay than did supernatant from live cells. Similarly, supernatant from apoptotic cells introduced into a subcutaneous air pouch in mice recruited more macrophages than did supernatant from live cells. Treatment of the supernatant from the apoptotic cells with apyrase, which hydrolyzes nucleoside triphosphates and diphosphates, blocked the recruitment of macrophages to the air ...http://stke.sciencemag.org/content/2/88/ec302
MiRs influence the PELP1-mediated migratory and invasio | Open-i
MiRs influence the PELP1-mediated migratory and invasion potential. A, Boyden chamber analysis of the cell migration potential of the ZR cells transfected withhttps://openi.nlm.nih.gov/detailedresult.php?img=PMC3935988_nihms550214f2&req=4
Cell Adhesion and Cell Sorting Cell IdentityMorphogenesis ? Cell Biology Cell Division/Death Cell Adhesion Cell Movement ...
Getting cells inside Cell Movements Relevant for Gastrulation Spreading tissues out Making tissues longer Convergence/extension Moving cells aroundhttp://slideplayer.com/slide/4225559/
Applications of Corning® FluoroBlok™ Permeable Supports
In vitro cell migration and invasion assays are frequently used as model systems for studying the directed movement of cells towards a chemoattractant stimulus, or to determine how a particular drug, growth factor or extra cellular matrix coating affects ...https://www.brighttalk.com/webcast/6639/115469