*  Critical Role of MicroRNA-155 in Herpes Simplex Encephalitis | The Journal of Immunology
Viral plaque assay. Virus titers were measured in the brain, TG, and skin of HSV-infected mice as described previously by ... D) The primary site of skin inoculation was examined for viral titers at day 6 p.i. using plaque assay. The level of ... by swabbing the HSV-infected eye with a sterile swab and assaying for the virus by plaque assay. The level of significance was ... Virus titers in all samples were measured using standard plaque assay as described previously (24). ...
  http://www.jimmunol.org/content/192/6/2734
*  Defining limits of treatment with humanized neutralizing monoclonal antibody for West Nile virus neurological infection in a...
Viral Plaque Assay. West Nile Fever / drug therapy*, immunology, mortality. West Nile virus / immunology*. ... Previous Document: A novel fluorescence intensity screening assay identifies new low-molecular-weight inhibitors of the.... ... Overall, these data suggest that in hamsters hE16 can ameliorate neurological disease after significant viral replication has ...
  http://www.biomedsearch.com/nih/Defining-limits-treatment-with-humanized/17452485.html
*  sodium bentonite sales in indiana
Vesicular stomatitis Indiana virus/drug effects Viral Plaque Assay/methods* .>More details ... Interferon titration by means of plaques under a bentonite .. [Interferon titration by means of plaques under a bentonite layer ...
  http://hica.co.za/11-2015/32466-sodium-bentonite-sales-in-indiana.html
*  NIOSHTIC-2 Publications Search - 20042261 - High humidity leads to loss of infectious influenza virus from simulated coughs.
At constant temperature, the RH was varied from 7-73% and infectivity was assessed by the viral plaque assay. Results: Total ... Humidity; Aerosols; Infectious-diseases; Diseases; Disease-vectors; Biological-effects; Statistical-analysis; Viral-diseases ...
  https://www.cdc.gov/niosh/nioshtic-2/20042261.html
*  9012-36-6 - Agarose LM, low melt, for recovery of samples after separation - H26417 - Alfa Aesar
Ideal for a variety of applications such as in-gel manipulation, tissue and cell culture, and viral plaque assays. Can also be ... used in protein electrophoresis applications such as Ouchterlony (antigen-antibody interaction assay) and radial ...
  https://www.alfa.com/en/catalog/H26417/
*  Insect Expression Support-Troubleshooting | Thermo Fisher Scientific - US
... take a P1 viral stock and redo the plaque assay. Be sure to select well-spaced, occ- plaques. If you are having difficulty ... I'm yielding no plaques from my baculovirus plaque assay. What are the possible causes for this? ... The viral titer is too low: Use a higher viral titer. You may need to re-infect your cells and collect a higher titer of your ... Viral stock contains a mixture of recombinant and non-recombinant baculovirus: Perform plaque purification to isolate ...
  http://www.thermofisher.com/us/en/home/technical-resources/technical-reference-library/protein-expression-support-center/insect-expression-support/insect-expression-support-troubleshooting.html
*  Microcrystalline cellulose - Wikipedia
"New low-viscosity overlay medium for viral plaque assays". Virology Journal. BioMed Central. 3: 63. doi:10.1186/1743-422X-3-63 ... It is also used in plaque assays for counting viruses, as an alternative to carboxymethylcellulose. A naturally occurring ...
  https://en.wikipedia.org/wiki/Microcrystalline_cellulose
*  Analysis of frameshifting in the viral context. (A) Rad | Open-i
Analysis of frameshifting in the viral context. (A) Radiolabeled TMEV translation products. BHK-21 cells were infected with ... UUU shift site to inhibit frameshifting results in an attenuated virus with reduced growth kinetics and a small-plaque ... Figure 3: Analysis of frameshifting in the viral context. (A) Radiolabeled TMEV translation products. BHK-21 cells were ... Figure 3: Analysis of frameshifting in the viral context. (A) Radiolabeled TMEV translation products. BHK-21 cells were ...
  https://openi.nlm.nih.gov/detailedresult.php?img=PMC4524249_zjv9990906770003&req=4
*  Frontiers | Enforced OX40 Stimulation Empowers Booster Vaccines to Induce Effective CD4+ and CD8+ T Cell Responses against...
... that the induction of strong T cell responses can be a fundamental component in the design of vaccines against persistent viral ... a mixture of SLPs containing MHC class II epitopes and OX40 agonistic antibodies resulted in a moderate reduction of the viral ... a mixture of SLPs containing MHC class II epitopes and OX40 agonistic antibodies resulted in a moderate reduction of the viral ... The viral titers of the produced virus stocks were determined by viral plaque assays with mouse NIH-3T3 Embryonic Fibroblasts ( ...
  https://www.frontiersin.org/articles/10.3389/fimmu.2017.00144/full
*  Laboratory Manual of Microbiology
Determination of viral plaque assay. 80. The one step bacteriophage growth curve. 81. Kirby-Bauer disk diffusion susceptibility ...
  http://www.scientificpub.com/book-details/Laboratory-Manual-of-Microbiology-93.html
*  Texaphyrin metal complexes having improved functionalization - Patent # 5599923 - PatentGenius
... viral inactivation as judged by viral plaque assay. As shown by mitogenic assay, aerobic photosensitization of cellsexposed to ... Similarly, texaphyrin complexes of choice may be added to standard viral culturesand the rate of viral growth retardation ... Viral Inactivation by Texaphyrin Macrocycles. One aspect of the utility of the present invention is the use of complexes ... In blood, an exemplary viral deactivating method would include: i) mixing with blood in vitro or ex vivo a texaphyrin metal ...
  http://www.patentgenius.com/patent/5599923.html
*  Human-murine hybridoma fusion partner - The Board of Trustees of the Leland Stanford Junior University
... and in the viral plaque reduction assay of paragraph C.8.d, showed neutralization at 5 μg/ml of protein. One of these subclones ... VZV antibody was assessed by the plaque reduction multiplicity analysis. The assay is as described by Grose, et al, J Infect ... The following paragraphs set forth assay procedures useful in the illustrations of the Invention. C.8.a. Assay for ... and successful clones were assayed for immunoglobulin production and for HLA surface proteins using the assay methods as set ...
  http://www.freepatentsonline.com/4634666.html
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
The antiviral activity was evaluated against HSV-1 and HSV-2 by a viral plaque number reduction assay (IC(50) ) and the ... The most active fraction was selected and assayed to determine the viral multiplication steps upon which it acted. In order to ... Serum-resting, fasted leptin levels were measured by Immunoradiometric Assay (IRMA), and leptin-to-fat mass ratios were ... were used for gastric mucin binding and HeLa cell invasion assays, or for in vivo experiments. Metacyclic trypomastigotes and ...
  https://www.jove.com/visualize?author=Vanessa+M+D%27Costa
*  Protein Expression in the Baculovirus System - Current Protocols
Support Protocol 1: Titrating Viral Stocks with the Plaque Assay. *Support Protocol 2: Titrating Viral Stocks with the End‐ ... a plaque assay and an end‐point assay). Once viral stocks have been prepared and titered, a protocol for testing the virus in ... Support Protocol 2: Titrating Viral Stocks with the End‐Point Assay. Materials * Sf9 cells (1 × 106 cells/ml), grown in Sf‐900 ... Basic Protocol 1: Large‐Scale Production of Viral Stock. MaterialsSpodoptera frugiperda (Sf9) cells * Insect cell medium: TC100 ...
  http://www.currentprotocols.com/WileyCDA/CPUnit/refId-ps0505.html?quicktabs_cp=toc
*  'protein subunits' Protocols and Video...
Viral Concentration Determination Through Plaque Assays: Using Traditional and Novel Overlay Systems', 'Optimized Negative ... Viral Nanoparticles for In vivo Tumor Imaging', 'Production of Disulfide-stabilized Transmembrane Peptide Complexes for ... G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay', 'Synthesis of ... Structural Studies', 'Coupled Assays for Monitoring Protein Refolding in Saccharomyces cerevisiae', 'Imaging the Neutrophil ...
  https://www.jove.com/keyword/protein+subunits
*  'encephalitis virus western equine' Protocols and Video...
Viral Concentration Determination Through Plaque Assays: Using Traditional and Novel Overlay Systems', 'Intracerebroventricular ... Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins', 'Thermostabilization, Expression, ... High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in ... Viral Nanoparticles for In vivo Tumor Imaging', 'Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins ...
  https://www.jove.com/keyword/encephalitis+virus+western+equine
*  Protocols and Video Articles Authored by Kathleen A. Kuehl (Translated to Korean)
... including a comparison of STEM-VQ to viral plaque assay and the ViroCyt Virus Counter. ... Preparation of Viral Samples Within Biocontainment for Ultrastructural Analysis: Utilization of an Innovative Processing ... Cross-protection Conferred by Filovirus Virus-like Particles Containing Trimeric Hybrid Glycoprotein Viral Immunology. Feb, ... quantitation is a valuable technique because it provides direct morphology information and counts of all viral particles, ...
  https://www.jove.com/author/Kathleen+A._Kuehl?language=Korean
*  Protocols and Video Articles Authored by Kathleen A. Kuehl
Utilization of Capsules for Negative Staining of Viral Samples within Biocontainment' ... including a comparison of STEM-VQ to viral plaque assay and the ViroCyt Virus Counter. ... Preparation of Viral Samples Within Biocontainment for Ultrastructural Analysis: Utilization of an Innovative Processing ... Utilization of Capsules for Negative Staining of Viral Samples within Biocontainment Candace D. Blancett1, Mitchell K. ...
  https://www.jove.com/author/Kathleen+A._Kuehl
*  Protocols and Video Articles Authored by Mei G. Sun (Translated to Spanish)
... including a comparison of STEM-VQ to viral plaque assay and the ViroCyt Virus Counter. ... Preparation of Viral Samples Within Biocontainment for Ultrastructural Analysis: Utilization of an Innovative Processing ... quantitation is a valuable technique because it provides direct morphology information and counts of all viral particles, ...
  https://www.jove.com/author/Mei+G._Sun?language=Spanish
*  Protocols and Video Articles Authored by Mei G. Sun (Translated to Portuguese)
Utilização de Cápsulas para Coloração Negativa de Amostras Viral dentro de Biocontenimento' ... including a comparison of STEM-VQ to viral plaque assay and the ViroCyt Virus Counter. ... Utilização de Cápsulas para Coloração Negativa de Amostras Viral dentro de Biocontenimento Candace D. Blancett1, Mitchell K. ... Preparation of Viral Samples Within Biocontainment for Ultrastructural Analysis: Utilization of an Innovative Processing ...
  https://www.jove.com/author/Mei+G._Sun?language=Portuguese
*  Protocols and Video Articles Authored by Candace D. Blancett (Translated to Dutch)
... including a comparison of STEM-VQ to viral plaque assay and the ViroCyt Virus Counter. ... Clinical laboratory assays that analyze blood or other biological fluids are the highest priority because these specimens can ... Preparation of Viral Samples Within Biocontainment for Ultrastructural Analysis: Utilization of an Innovative Processing ... However, progress in developing sensitive assays for monitoring B. mallei infection is hampered by a shortage of useful ...
  https://www.jove.com/author/Candace+D._Blancett?language=Dutch
*  Insect Expression Support-Getting Started | Thermo Fisher Scientific
We recommend you perform a plaque assay to determine the titer of your viral stock. You may also perform a plaque assay to ... So, if you have a well with viral dilution of 10-8 containing 18 white plaques, the viral titer is calculated as followed:. X ... Count plaques on a dilution plate. (1/dilution) x # of plaques = pfu/mL. e.g., if you have 50 plaques on the 10-6 plate, then ... Thus, recombinant Bac-N-Blue™ baculovirus will provide blue plaques in the plaque assay and can be easily identified. In the ...
  https://www.thermofisher.com/us/en/home/technical-resources/technical-reference-library/protein-expression-support-center/insect-expression-support/insect-expression-support-getting-started.html
*  Virus quantification - Wikipedia
Viral plaque assays determine the number of plaque forming units (pfu) in a virus sample, which is one measure of virus ... as these viruses would not be amenable to the plaque assay. Like the plaque assay, host cell monolayers are infected with ... The focus forming assay (FFA) is a variation of the plaque assay, but instead of relying on cell lysis in order to detect ... For example, the production of viral vaccines, recombinant proteins using viral vectors and viral antigens all require virus ...
  https://en.wikipedia.org/wiki/Virus_quantification
*  Infection-dependent phenotypes in MHC-congenic mice are not due to MHC: can we trust congenic animals? | BMC Immunology | Full...
TMEV titers were assayed by conducting viral plaque assays on brain and spinal cord homogenates [24]. Briefly, brain and spinal ... A subsequent study using an odor detection assay with mice confirmed that these two congenic strains did have odor differences ... Salmonella titers were assayed from platings of homogenized spleen. Briefly, spleens were collected under antiseptic conditions ... F1 and F2 mice were assayed for differences in weight gain and pathogen titer in this coinfection model. We report significant ...
  https://bmcimmunol.biomedcentral.com/articles/10.1186/1471-2172-5-14
*  A Critical Role for Induced IgM in the Protection against West Nile Virus Infection | JEM
2 D). In wild-type mice, kidney viral burdens were below the level of detection by direct plaque assay at all times measured. ... However, when viral RNA in serum was measured by a more sensitive fluorogenic RT-PCR assay (11, 42), additional information was ... days after subcutaneous footpad inoculation with 102 PFU of WNV and virus levels were measured using a viral plaque assay in ... The dotted line represents the limit of sensitivity of the assay. (B) WNV RNA levels in serum. Viral RNA levels were determined ...
  http://jem.rupress.org/content/198/12/1853