Viola phlebovirus is a novel Phlebotomus fever serogroup member identified in Lutzomyia ( Lutzomyia) longipalpis from Brazilian...
In addition, viral isolation of Viola virus in mammalian cell lines (Vero cells) and the presence of NSs and NSm proteins ... Viral isolation in Vero cell culture, RT-PCR and transmission electron microscopy. A dilution (1:10 in 1 ml of inoculum) in ... viral isolation of a putative Phlebovirus positive pool in Vero cells, RT-PCR and transmission electron microscopy (TEM). ... This phlebovirus was isolated in Vero cells, confirmed by TEM and RT-PCR for the L segment of the virus, and named Viola ...https://link.springer.com/article/10.1186%2Fs13071-018-2985-3
ProbesOnline, Fluorescence Imaging, Tracking Tumor Metastasis, Chemiluminescence Assays | Thermo Fisher Scientific
Subcellular localization of viral RNA transcription in vero cells. Vero cells were pretreated with 2 µM actinomycin D to ... Countess™ Automated Cell Counter. each. C10227. Countess™ Automated Cell Counter Starter Kit (includes 1 cell counter and 11 ... Countess™ Cell Counting Chamber Slides. 500 slides (1,000 counts). C10312. Countess™ Cell Counting Chamber Slides. 1,250 slides ... Countess™ Cell Counting Chamber Slides. 2,500 slides (5,000 counts). C10314. Countess™ Cell Counting Chamber Slides. 5,000 ...https://www.thermofisher.com/cl/en/home/references/newsletters-and-journals/probesonline/probesonline-issues-2009/probesonline-july-2009.html
Aiamkitsumrit B[au] - PubMed - NCBI
The stability of herpes simplex virus type I genomes in infected Vero cells undergoing viral induced apoptosis. ... Development of co-selected single nucleotide polymorphisms in the viral promoter precedes the onset of human immunodeficiency ... The receptor repertoire and functional profile of follicular T cells in HIV-infected lymph nodes. ... type 1 ICP4 deletion mutant virus d120 infection failed to induce apoptosis in nerve growth factor-differentiated PC12 cells. ...https://www.ncbi.nlm.nih.gov/pubmed?access_num=Aiamkitsumrit+B&link_type=AUTHORSEARCH&cmd=search&term=Aiamkitsumrit+B%5Bau%5D&dispmax=50
Rubella IgM MAX™ Antigen
Grown in VERO cells. • Viral Strain HPV77. • Suitable for use in CLIA, ELISA, and WB. ... It is prepared by extraction of Rubella antigens from cells infected with Rubella Virus strain HPV77 using a patented process ( ...https://meridianlifescience.com/news/rubella-igm-max-antigen.html
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Table 2.2: Anti-Viral Vero Cell-Based Vaccines *Table 3.1: Vaccines: Clinical Trials and Pipeline Data *Table 3.2: Cancer ... 2.1 Viral Vaccine Production: Eggs Versus Cell Culture *2.2 Cell Line Options *2.3 Conventional Vaccines *2.4 Adjuvants *2.5 ... 3.2.4 Renal Cell Carcinoma *3.2.5 Melanoma: Dendritic Cells *3.2.6 Prostate Cancer CHAPTER 4 Big Pharma Vaccinology. *4.1 ... 2.7 Vaccines Driving T-Cell Activation *2.8 Dendritic Cells CHAPTER 3 Vaccine Targets. *3.1 Intectious Diseases *3.1.1 ...http://www.giichinese.com.tw/report/cd298556-vaccines-end-illness-overview.html
Prophylaxis and immunization in mice by use of virus-free defective T particles to protect against intracerebral infection by...
... virus-free T particles at extremely high multiplicities depress cellular RNA and protein synthesis and kill BHK21 cells in ... Defective interfering T particles of vesicular stomatitis virus provide remarkable protection against viral disease and death ... Accumulation of defective interfering viral particles in only a few passages in Vero cells attenuates mumps virus ... Defective interfering T particles of vesicular stomatitis virus provide remarkable protection against viral disease and death ...https://www.semanticscholar.org/paper/Prophylaxis-and-immunization-in-mice-by-use-of-T-to-Doyle-Holland/84ebd7018d23e12bce87af9acaf075a09911414c
West Nile Virus from Blood Donors, Vertebrates, and Mosquitoes, Puerto Rico, 2007 - Volume 15, Number 8-August 2009 - Emerging...
Viral RNA was extracted from first passage Vero cell supernatant using the QIAamp Viral RNA kit (QIAGEN, Valencia, CA, USA). RT ... or Vero (African green monkey kidney) cell cultures with mosquito pool extract. ... Viral isolates from outside the United States were obtained from Mexico in 2003 and Argentina in 2006 (2,5). Although many ... Dr Hunsperger is a virologist and the chief of the Serology Diagnostics and Viral Pathogenesis Research Laboratory at the ...https://wwwnc.cdc.gov/eid/article/15/8/09-0333_article
β-d-N4-Hydroxycytidine Is a Potent Anti-alphavirus Compound That Induces a High Level of Mutations in the Viral Genome |...
... and viral titers were determined by a plaque assay on Vero cells. (D) A total of 5 × 105 Vero cells were infected with the ... A) Vero cells were infected with VEEV TC-83 at an MOI of 0.5 PFU/cell. At the time points indicated, cells were treated with 2 ... Vero cells in six-well Costar plates were infected at room temperature with VEEV TC-83 at an MOI of 20 PFU/cell. (A) Cells were ... B) Vero cells were infected with indicated mutant viruses at an MOI of 1 PFU/cell. Cells were incubated in the presence of 2 μM ...https://jvi.asm.org/content/92/3/e01965-17
New Vero-SF-ACF Cell Line for Viral Propagation. The new Vero-SF-ACF (ATCC® CCL-81.5™) is optimized to grow in the serum-free, ... RPTEC/TERT-OAT1 Cells. RPTEC/TERT-OCT2 Cells. OAT1 HEK 293T/17 Cells. ATCC offers OAT1 and OCT2 cells, which stably express ... We offer cells from the renal proximal convoluted tubule, the renal cortex, mixed epithelial cells. Theses cells are well- ... Browse Kidney Cell Lines. ATCC's holdings include a vast collection of cell lines derived from kidney, representing the normal ...https://www.atcc.org/en/Products/Cells_and_Microorganisms/Toxicology.aspx?utm_id=T170201124l1
Effects of cocktail of four local Malaysian medicinal plants (Phyllanthus spp.) against dengue virus 2 | BMC Complementary and...
The MNTD of both aqueous and methanolic extracts on Vero cells were 250.0 μg/ml and 15.63 μg/ml respectively. Phyllanthus ... including viral entry, viral transcription and translation regulations, cytoskeletal assembly, and cellular metabolisms. ... The differentially expressed proteins in the treated and infected cells were analysed with two dimensional gel electrophoresis ... A) Vero cells treated with aqueous cocktail extract of Phyllanthus and (B) Vero cells treated with methanolic cocktail extract ...https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-13-192
Yellow fever vaccine YF-17D activates multiple dendritic cell subsets via TLR2, 7, 8, and 9 to stimulate polyvalent immunity |...
Viral titers were calculated by plaque assay using Vero cells. Virus was inactivated by either incubating at 56°C for 1 h or UV ... 5 B) by cells incubated with media alone (presumably as a result of viral carry over) and the ensuing T cell responses to ... Plasmacytoid dendritic cell precursors/type I interferon-producing cells sense viral infection by Toll-like receptor (TLR) 7 ... T cell epitopes that had been previously identified (72). The CD4+ T cell epitope is an I-Ab-restricted epitope from the viral ...http://jem.rupress.org/content/203/2/413
Frontiers | Green Tea Catechin-Inactivated Viral Vaccine Platform | Microbiology
As the first report of using non-toxic natural compounds for the preparation of inactivated viral vaccines, the present results ... In a mouse model, vaccination with GT-inactivated influenza virus (GTi virus) elicited higher levels of viral neutralizing ... In a mouse model, vaccination with GT-inactivated influenza virus (GTi virus) elicited higher levels of viral neutralizing ... can be used as an inactivating agent to prepare inactivated viral vaccines. GT treatment resulted in complete and irreversible ...https://www.frontiersin.org/articles/10.3389/fmicb.2017.02469/full
Clinical Stage Products | VxP Biologics
The Vero cell platform enables rapid development of clinical-stage viral vaccines.. The Vero cell platform uses animal model ... Thus, the Vero cell development and industrial scale manufacturing platform was established to enable the rapid development of ... Also known as mAbs or moAbs, monoclonal antibodies are generated in clones of a cell, often from a human or rodent. They bind ... Beyond this, mAbs can also be engineered to deliver a specific cytokine, radioisotope or toxin to specific cells, or even to ...http://www.vxpbiologics.com/clinical-stage-products/
A Full-Length Infectious cDNA Clone of Zika Virus from the 2015 Epidemic in Brazil as a Genetic Platform for Studies of Virus...
Vero cell platform in vaccine production: moving towards cell culture-based viral vaccines. Expert Rev Vaccines 8:607-618. doi: ... Each cell line was infected at an MOI of 0.01 PFU/cell in duplicate. Titers were determined by plaque assay in Vero cells and ... Each cell line was infected at an MOI of 0.01 PFU/cell in duplicate. Titers were determined by plaque assay in Vero cells and ... Since Vero cells are used for commercial production of biological materials (38), we developed a Vero cell-adapted ZIKV clone, ...https://mbio.asm.org/content/7/4/e01114-16
JCVI: Isolation and Characterization of a Novel Gammaherpesvirus from a Microbat Cell Line.
Passage of supernatants from microbat cells to Vero cells resulted in syncytium formation, and expression of viral genes and ... Next-generation sequencing and de novo assembly of the viral genome from supernatants from Vero cells yielded a single contig ... This virus can replicate in select human and nonhuman primate cell lines. However, analyses of viral sequences support a bat ... amplification of viral DNA were demonstrated by quantitative PCR. Susceptibility of human cell lines to productive infection ...http://www.jcvi.org/cms/publications/listing/abstract/article/isolation-and-characterization-of-a-novel-gammaherpesvirus-from-a-microbat-cell-line-1/
... of either uninfected or virus-infected Vero cell lysates, or purified viral antigens or MBP at 10 μg/ml. These concentrations ... Few studies have examined T-cell responses to these viruses, and therefore the nature of the viral targets and the immune cells ... Wucherpfennig KW, Strominger JL: Molecular mimicry in T cell-mediated autoimmunity: viral peptides activate human T cell clones ... T-cells with the capacity to proliferate have been termed "central memory," or TCM cells. These cells lack immediate effector ...http://diabetes.diabetesjournals.org/content/51/6/1745
CiNii 論文 - Japanese Encephalitis Virus Infection in Vero Cells:The Involvement of...
Flavivirus infection enhancement in macrophages:an electron microscopic study of viral cellular entry GOLLINS S. W. ... Possible involvement of receptors in the entry of Kunjin virus into Vero cells NG M. L. ... The cytotoxic action of diphtheria toxin and its degradation in intact Vero cells are inhibited by bafilomycin A1, a specific ... Japanese Encephalitis Virus Infection in Vero Cells:The Involvement of Intracellular Acidic Vesicles in the Early Phase of ...http://ci.nii.ac.jp/naid/10004897252
ASMscience | Functions of Antibodies
In addition, in vivo models, particularly those engineered to knock in or knock out effector cells or effector molecules, are ... Antibodies can impact pathogens in the presence or in the absence of effector cells or effector molecules such as complement, ... Neutralizing monoclonal antibody to the E1 glycoprotein epitope of rubella virus mediates virus arrest in VERO cells. Viral ... Intracellular neutralization of viral infection in polarized epithelial cells by neonatal Fc receptor (FcRn)-mediated IgG ...http://www.asmscience.org/content/journal/microbiolspec/10.1128/microbiolspec.AID-0019-2014
Severe Fever with Thrombocytopenia Syndrome, South Korea, 2012 - Volume 19, Number 11-November 2013 - Emerging Infectious...
RNA was extracted from the stored blood and from virus-infected Vero cells by using a QIAamp Viral RNA Mini Kit (QIAGEN, Hilden ... we inoculated monolayers of Vero cells with the patient's blood sample and cultured the cells at 37°C in a 5% carbon dioxide ... we observed a cytopathic effect of SFTSV in DH82 cells. The SFTSV-infected Vero cell monolayer was fixed according to described ... Transmission electron microscopy image of Vero cells infected with severe fever with thrombocytopenia syndrome virus (arrows). ...https://wwwnc.cdc.gov/eid/article/19/11/13-0792_article
Vaccines - Eppendorf
Vero cells are anchorage-dependent cells that are widely used as a platform for viral vaccine production. In stirred-tank ... The cultivation of Vero cells in benchtop single-use and glass bioreactors with a packed bed made of Fibra-Cel disks has been ... Viral vector production. Viral vectors are used in molecular biology research, gene therapy applications, and in vaccine ... From Cell Seeding to Analysis - Getting the best out of your cell-based assay ...https://www.eppendorf.com/RU-ru/primenenie/bioprocess/vaccines/
Immunogenicity and Safety of Inactivated Vero Cell Derived Japanese Encephalitis Vaccine in Thai Children - Full Text View -...
Encephalitis, Viral. Central Nervous System Viral Diseases. RNA Virus Infections. Flavivirus Infections. Flaviviridae ... Immunogenicity and Safety of Inactivated Vero Cell Derived Japanese Encephalitis Vaccine in Thai Children (JE0153). This study ... This study aims to evaluate the immunogenicity and safety of inactivated Vero cell derived JE vaccine (Beijing P-3 strain) ... Immunogenicity and Safety of Inactivated Vero Cell Derived Japanese Encephalitis Vaccine in Thai Children. ...https://clinicaltrials.gov/ct2/show/NCT01408537
Impaired induction of IL-15 in response to herpes simplex vi... : AIDS
... viral preparation that was produced in VERO cells and contained 1 × 108 plaque-forming units per millilitre. After infection at ... Impaired in vitro interferon, blastogenic and natural killer cell responses to viral stimulation in acquired immune deficiency ... However, unlike IL-2, which is produced by activated T cells, IL-15 is produced from a wide variety of cells and tissues in the ... IL-15 is induced in the host as a defence to viral and non-viral intracellular pathogens. Human herpesviruses have been ...https://journals.lww.com/aidsonline/Fulltext/2000/04140/Impaired_induction_of_IL_15_in_response_to_herpes.15.aspx
Zika virus inhibits eIF2α-dependent stress granule assembly
Vero cells were seeded on 18 mm coverslips and infected as described above. Viral RNA was labeled as described in . Briefly ... Blue arrows: uninfected cells; red arrows: infected cells. B. At least 150 cells in each condition were analyzed. Cells with at ... To produce viral stocks, Vero cells were infected with ZIKV strain PRVABC59/2015 at a multiplicity of infection (MOI) of 0.01 ... U2OS cells were used in place of Vero cells due to the high toxicity of Se to the latter ones. SG assembly was determined by ...http://pubmedcentralcanada.ca/pmcc/articles/PMC5531678/
ASMscience | Vaccines for Severe Acut
... and T-cell responses. Then, it discusses different types of vaccines under development, including inactivated viruses, subunit ... Viral titers in the lungs were determined in Vero-E6 cell monolayers. The nonparametric Mann-Whitney U statistical method was ... Viral titers in the lungs were determined in Vero-E6 cell monolayers. The nonparametric Mann-Whitney U statistical method was ... A) Vero-E6 cells were infected at a multiplicity of infection of 0.5 with either the rSARS-CoV-ΔE or the recombinant wild-type ...http://www.asmscience.org/content/book/10.1128/9781555815790.ch25
Vero cells were used for virus propagation, detection, or enumeration of infectious viral particles and for viral- ... Although NK cells, CD3+/4+ T cells, and B cells were similar in number between the groups, CD3+/8+ T cells were significantly ... 106 cells/mL. The Jurkat human acute T-cell lymphoblastic leukemia cell line (from ATCC), the human acute immunoblastic B-cell ... Enumeration of B cell (CD19+), T cell (CD3+/CD4+ and CD3+/CD8+), and NK cell (NK1.1+) subpopulations was conducted on CyAn ADP ...http://clincancerres.aacrjournals.org/content/19/14/3832