*  Occurrence of integrons and resistance genes among sulphonamide-resistant Shigella spp. from Brazil - DTU Orbit
Objectives: To determine the occurrence of class 1 and 2 integrons and antimicrobial resistance genes among sulphonamide-resistant Shigella strains isolated in Brazil during 1999-2003. Methods: Sixty-two Shigella (Shigella flexneri, n = 47 and Shigella sonnei, n = 15) were tested against 21 antimicrobial agents. The presence of integrons classes 1 and 2 and antimicrobial resistance genes was investigated by PCR using specific primers. Results: A total of eight antimicrobial resistance profiles were identified, with the profile of resistance to sulfamethoxazole, trimethoprim, spectinomycin, streptomycin and tetracycline being the most common among S. sonnei, and additionally to ampicillin and chloramphenicol among S. flexneri. Class 1 integrons were found in only two strains, whereas class 2 integrons were found in 56 (90.3%) of the strains. All class 2-positive strains had a similar fragment of 2214 bp harbouring a gene cassette array ...
  http://orbit.dtu.dk/en/publications/occurrence-of-integrons-and-resistance-genes-among-sulphonamideresistant-shigella-spp-from-brazil
*  Integron, Plasmid and Host Strain Characteristics of Escherichia coli from Humans and Food Included in the Norwegian...
Antimicrobial resistant Escherichia coli (n=331) isolates from humans with bloodstream infections were investigated for the presence of class 1 and class 2 integrons. The integron cassettes arrays were characterized and the findings were compared with data from similar investigations on resistant E. coli from meat and meat products (n=241) produced during the same time period. All isolates were obtained from the Norwegian monitoring programs for antimicrobial resistance in human pathogens and in the veterinary sector. Methods used included PCR, sequencing, conjugation experiments, plasmid replicon typing and subtyping, pulsed-field-gel-electrophoresis and serotyping. Integrons of class 1 and 2 occurred significantly more frequently among human isolates; 45.4% (95% CI: 39.9-50.9) than among isolates from meat; 18% (95% CI: 13.2 -23.3), (p|0.01, Chi-square test). Identical cassette arrays including dfrA1-aadA1, aadA1, dfrA12-orfF-aadA2, oxa-30-aadA1 (class 1 ...
  http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0128797
*  Etymologia: Verona Integron - Volume 19, Number 7-July 2013 - Emerging Infectious Disease journal - CDC
Verona Integron. From the Latin integrare (to make whole), integrons are systems for capturing and spreading antibiotic resistance genes among gram-negative bacteria. Integrons were first described by Stokes and Hall in 1989, although they clearly contributed to the first outbreaks of multidrug resistance in the 1950s. The Verona integron was first described in carbapenem-resistant Pseudomonas aeruginosa isolated from a patient hospitalized at Verona University Hospital, Verona, Italy. Integrons are ancient structures that have been present in bacteria for millions of years, indicating that bacteria had the means of acquiring and disseminating antibiotic resistance long before humans developed antibiotics.. ...
  https://wwwnc.cdc.gov/eid/article/19/7/et-1907_article
*  PLOS Pathogens: Natural Transformation Facilitates Transfer of Transposons, Integrons and Gene Cassettes between Bacterial...
Author Summary Genetic elements, such as transposons and integrons, frequently carry antimicrobial resistance determinants and can be found widely disseminated among pathogenic bacteria. Their distribution pattern suggests dissemination through horizontal gene transfer. The role of natural transformation in horizontal transfer of genetic elements other than those that are self-replicative (plasmids) has remained largely unexplored. We have tested if natural transformation can facilitate transfer of transposons and class 1 integrons between bacterial species. We here provide experimental evidence showing that natural transformation can be a general mechanism for dissemination of genetic elements that by themselves do not encode interspecies transfer functions (e.g. transposons, insertion sequences). We demonstrate that antibiotic resistance determinants present in such genetic elements can spread by natural transformation between species of clinical interest. We show by ...
  http://journals.plos.org/plospathogens/article/authors?id=10.1371/journal.ppat.1002837
*  Distribution of Tetracycline and Streptomycin Resistance Genes and Class 1 Integrons inEnterobacteriaceaeIsolated from Dairy...
The prevalence of selected tetracycline and streptomycin resistance genes and class 1 integrons in Enterobacteriaceae (n = 80) isolated from dairy farm soil and nondairy soils was evaluated. Among 56 bacteria isolated from dairy farm soils, 36 (64.3%) were resistant to tetracycline, and 17 (30.4%) were resistant to streptomycin. Lower frequencies of tetracycline (9 of 24 or 37.5%) and streptomycin (1 of 24 or 4.2%) resistance were observed in bacteria isolated from nondairy soils. Bacteria (n = 56) isolated from dairy farm soil had a higher frequency of tetracycline resistance genes including tetM (28.6%), tetA (21.4%), tetW (8.9%), tetB (5.4%), tetS (5.4%), tetG (3.6%), and tetO (1.8%). Among 24 bacteria isolated from nondairy soils, four isolates carried tetM, tetO, tetS, and tetW in different combinations; whereas tetA, tetB, and tetG were not detected. Similarly, a higher prevalence of streptomycin resistance genes including strA (12.5%), strB (12.5%), ant(3″) (12.5), aph(6)-1c (12.5%), ...
  https://link.springer.com/article/10.1007%2Fs00248-007-9266-6
*  Structure of In31, a bla(IMP)-containing Pseudomonas aeruginosa integron phyletically related to In5, which carries an unusual...
The location and environment of the acquired bla(IMP) gene, which encodes the IMP-1 metallo-β-lactamase, were investigated in a Japanese Pseudomonas aeruginosa clinical isolate (isolate 101/1477) that produced the enzyme. In this isolate, bla(IMP) was carried on a 36-kb plasmid, and similar to the identical alleles found in Serratia marcescens and Klebsiella pneumoniae clinical isolates, it was located on a mobile gene cassette inserted into an integron. The entire structure of this integron, named In31, was determined. In31 is a class 1 element belonging to the same group of defective transposon derivatives that originated from Tn402-like ancestors such as In0, In2, and In5. The general structure of In31 appeared to be most closely related to that of In5 from pSCH884, suggesting a recent common phylogeny for these two elements. In In31, the bla(IMP) cassette is the first of an array of five gene cassettes that also includes an aacA4 cassette and three original cassettes that have never been ...
  https://ricerca.univaq.it/handle/11697/103073
*  Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ | BMC Genomics | Full Text
Although we previously developed an improved CRISPR/Cas system for HR-mediated gene cassette knock-in in mice by using chemically synthesized dual-RNAs and commercially available Cas9 protein [8], constructing targeting vectors containing long homology arms corresponding to every target locus has remained cumbersome and fundamentally unchanged for 30 years [2]. In this report, we applied the PITCh system for the generation of knock-in mice, and this resulted in the omission of the laborious steps of constructing targeting vectors. Our new method has major advantages for researchers generating gene cassette knock-in mice.. First, our method enables extremely convenient and high-throughput donor vector production. Because the MMEJ-directed donor vector can easily be prepared by a single PCR and TA-cloning using primers conjugated with 40-bp microhomology and gRNA-s1 crRNA target sequence from any template plasmid containing various gene cassettes (EGFP, Cre, and any functional gene cassettes) ...
  https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-016-3331-9
*  A novel family of potentially mobile DNA elements encoding site-specific gene-integration functions: integrons - Stokes - 1989 ...
A family of novel mobile DNA elements is described, examples of which are found at several independent locations and encode a variety of antibiotic resistance genes. The complete elements consist of two conserved segments separated by a segment of variable length and sequence which includes inserted antibiotic resistance genes. The conserved segment located 3′ to the inserted resistance genes was sequenced from Tn21 and R46, and the sequences are identical over a region of 2026 bases, which includes the sulphonamide resistance gene sull, and two further open reading frames of unknown function. The complete sequences of both the 3′ and 5′ conserved regions of the DNA element have been determined. A 59-base sequence element, found at the junctions of inserted DNA sequences and the conserved 3′ segment, is also present at this location in the R46 sequence. A copy of one half of this 59-base element is found at the end of the sull gene, suggesting that sull, though part of the conserved ...
  http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.1989.tb00153.x/abstract
*  Identification of acquired antimicrobial resistance genes - DTU Orbit
ObjectivesIdentification of antimicrobial resistance genes is important for understanding the underlying mechanisms and the epidemiology of antimicrobial resistance. As the costs of whole-genome sequencing (WGS) continue to decline, it becomes increasingly available in routine diagnostic laboratories and is anticipated to substitute traditional methods for resistance gene identification. Thus, the current challenge is to extract the relevant information from the large amount of generated data.MethodsWe developed a web-based method, ResFinder that uses BLAST for identification of acquired antimicrobial resistance genes in whole-genome data. As input, the method can use both pre-assembled, complete or partial genomes, and short sequence reads from four different sequencing platforms. The method was evaluated on 1862 GenBank files containing 1411 different resistance genes, as well as on 23 de-novo-sequenced isolates.ResultsWhen testing the 1862 GenBank files, the method identified the resistance ...
  http://orbit.dtu.dk/en/publications/identification-of-acquired-antimicrobial-resistance-genes
*  2014年 | 日本食・発酵食品の革新的研究開発拠点
発表年 発表論文名・著書名等 2014年 Ahmed, A. M., Shimamoto, T., & Shimamoto, T. Characterization of integrons and resistance genes in multidrug-resistant
  http://rcjf.hiroshima-u.ac.jp/achievement/per_year/year2014/
*  Integron unveils its new Wireless Managed Services (WMS) platform | Comms Business
Integron unveils its new Wireless Managed Services (WMS) platform for end-to-end visibility and control of M2M wireless assets". Integron, the global M2M wireless deployment services company, today announced the release of its new Wireless Managed Services - WMS™ platform. The platform integrates wireless session/transaction data from multiple Carriers/MVNOs and critical asset management information that gives clients end-to-end visibility and control of their wireless assets.. Exhibiting at the 2012 American Telemedicine Association Meeting & Exposition, Integron demonstrated the new platform's capabilities in supporting the deployment of home telehealth and remote patient monitoring solutions. The new WMS™ platform provides the client with an comprehensive view of their telehealth device's wireless and asset status.. device wireless usage and detailed session data history (CDR). rate plan and wireless transactions. integrated fleet usage and billing (across multiple wireless ...
  http://commsbusiness.co.uk/news/integron-unveils-its-new-wireless-managed-services-wms-platform/
*  Catalogue of antibiotic resistome and host-tracking in drinking water deciphered by a large scale survey | Microbiome | Full...
The overuse and misuse of antibiotics, not only for human therapy, but also for livestock breeding around the world over the past decades have led to the emergence and excess of antibiotic resistance genes (ARGs) and antibiotic resistant bacteria (ARB) in a diverse range of environments [1-4]. Among the various ARB and ARG reservoirs, aquatic ecosystems are considered to be the most important due to the high mobility of organisms and genetic elements [5]. ARGs have been reported to rapidly spread, conducted by mobile genetic elements in ecosystems [6]. The rapid transfer and spread of ARGs among bacterial cells [7] could be facilitated by mobile genetic elements, including plasmids, transposons and integrons, etc. Recently, findings on ARGs in drinking water distribution systems, especially in treated drinking water that may have direct contact with human beings, have given rise to medical concerns from both researchers and the public [8-10]. Although most microorganisms can be effectively ...
  https://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-017-0369-0
*  Difference between revisions of "Mazel" - OpenWetWare
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  https://openwetware.org/wiki/?title=Mazel&diff=630552&oldid=284914
*  Difference between revisions of "Mazel" - OpenWetWare
margin: -240px 0px 0px 50%; } .selflink{ color: #ffffff; font-weight: normal; } ,/style, ,meta name='keywords' content='BGP, lab, laboratory, BGP lab, Bacterial Genome Plasticity, Super, Integrons, superintegron, bacterial, genome, plasticity, gene, institut Pasteur, institute, vibrio, cholerae, gene cassette, antibiotics, resistance' /, ,/html, ...
  https://openwetware.org/wiki/?title=Mazel&diff=630552&oldid=404069
*  Difference between revisions of "Mazel" - OpenWetWare
margin: -240px 0px 0px 50%; } .selflink{ color: #ffffff; font-weight: normal; } ,/style, ,meta name='keywords' content='BGP, lab, laboratory, BGP lab, Bacterial Genome Plasticity, Super, Integrons, superintegron, bacterial, genome, plasticity, gene, institut Pasteur, institute, vibrio, cholerae, gene cassette, antibiotics, resistance' /, ,/html, ...
  https://openwetware.org/wiki/?title=Mazel&diff=694155&oldid=609830
*  Mazel - OpenWetWare
margin: -240px 0px 0px 50%; } .selflink{ color: #ffffff; font-weight: normal; } ,/style, ,meta name='keywords' content='BGP, lab, laboratory, BGP lab, Bacterial Genome Plasticity, Super, Integrons, superintegron, bacterial, genome, plasticity, gene, institut Pasteur, institute, vibrio, cholerae, gene cassette, antibiotics, resistance' /, ,/html, ...
  https://openwetware.org/wiki/?title=Mazel&oldid=787332
*  Microsoft word - main hall program final (eng)
Original Article High prevalence of trimethoprim-resistance cassettes in class 1 and 2 integrons in Senegalese Shigella spp isolates Amy Gassama-Sow 1 , Awa Aïdara-Kane 1§ , Olivier Barraud 2,3,4 , Martine Gatet 2,3 , François Denis 2,3,4 , and Marie-Cécile Ploy 2,3,4 1Unité de Bactériologie Expérimentale, Institut Pasteur, 220, Dakar, Sénégal 2Université de Limoge ...
  http://medpdffinder.com/i/iranathero.ir1.html
*  REI View
Definition : Proteus mirabilis strain PmAbb tRNA modification GTPase (thdF) gene, partial cds; Salmonella genomic island 1 variant SGI1-PmABB, complete sequence; transcriptional regulator (hipB) and regulatory protein (hipA) genes, complete cds; and membrane protein ...
  http://www.paidb.re.kr/view_rei.php?pn=Pro.1.5&m=r
*  NCTC 619 Strain Passport - StrainInfo
Clostridium acetobutylicum clone DC1494 CA_C1494 gene, partial sequence; pMTL007 integron, complete sequence; hypothetical protein (CA_C1492) gene, partial cds; and pMTL007 integron Erm resistance protein (ermB) gene, complete ...
  http://www.straininfo.net/strains/94108
*  Record details
When positioned on the flanking ends of gene cassettes, some the RB7 MAR has been shown to increase expression of transgenes and to reduce the incidence of gene silencing. It is hypothesized that MARs may act to buffer effects from neighbouring chromosomal sequences that could destabilize the expression of genes ...
  http://bch.cbd.int/database/record.shtml?documentid=104795
*  Stupid Mario Logic
... . WITH HI] fill! HMS INTI] WATER. Maybe he should install iOS7 Stupid Mario Logic WITH HI] fill! HMS INTI] WATER Maybe he should install iOS7
  http://www.funnyjunk.com/funny_pictures/4802733/Stupid+Mario+Logic/
*  SGI - Solutions
SGI is the world leader in providing high-performance computing solutions for scientific, engineering, and creative professionals.
  http://www.sgi.com/solutions/?/sciences/chembio/resources/htc/
*  GRAINGER APPROVED Cassette,1.629 In,PK500 - 21RK96|21RK96 - Grainger
Looking for GRAINGER APPROVED Cassette,1.629 In,PK500 (21RK96)? Grainger's got your back. Price:$51.50. Easy ordering & convenient delivery. Log-in or register for your pricing.
  https://www.grainger.com/product/GRAINGER-APPROVED-1-629-x-1-11-x-0-23-Polyoxymethylene-21RK96
*  Nige's Diet & Nutrition Blog: You are *never* going to guess what happened on Saturday night.
During the gig, a young lady asked the band if she could get up on stage and sing a song with them, to which they agreed. While the young lady was singing "Valerie" by Amy Winehouse, the lead singer stood by the table where I was sitting and put his glass on it. After a while, when he turned to pick up his glass, I attracted his attention and asked him "Can I have a go?" He asked me what I wanted to sing and I replied ""Sit down", by James ...
  http://nigeepoo.blogspot.com/2013/04/you-are-never-going-to-guess-what.html