Physical mobilization of secretory vesicles facilitates neuropeptide release by nerve growth factor-differentiated PC12 cells. ... Physical mobilization of secretory vesicles facilitates neuropeptide release by nerve growth factor-differentiated PC12 cells. ... Physical mobilization of secretory vesicles facilitates neuropeptide release by nerve growth factor-differentiated PC12 cells. ... based measurements in nerve growth factor-differentiated PC12 cells are used to test whether altering dense core vesicle (DCV) ...

... as well as in small synaptic vesicles (SSVs). This was discovered via studying rat adrenal medulla cells (PC12 cells). LDCVs ... "Preferential localization of a vesicular monoamine transporter to dense core vesicles in PC12 cells". J. Cell Biol. 127 (5): ... Specifically, VMAT1 is found in chromaffin cells, enterochromaffin cells, and small intensely fluorescent cells (SIFs). ... These intestinal endocrine cells process amine precursors, and VMAT1 pulls them into vesicles for storage. The activity of ...

Metabolic stress in PC12 cells induces the formation of the endogenous dopaminergic neurotoxin, 3,4-dihydroxyphenylacetaldehyde ... Here, we isolated for the first time, DA storage vesicles from the striatum of six autopsied brains of PD patients and four ... Is Parkinsons Disease a Vesicular Dopamine Storage Disorder? Evidence from a Study in Isolated Synaptic Vesicles of Human and ... Is Parkinsons Disease a Vesicular Dopamine Storage Disorder? Evidence from a Study in Isolated Synaptic Vesicles of Human and ...

Metabolic stress in PC12 cells induces the formation of the endogenous dopaminergic neurotoxin, 3,4-dihydroxyphenylacetaldehyde ... Metabolic stress in PC12 cells induces the formation of the endogenous dopaminergic neurotoxin, 3,4-dihydroxyphenylacetaldehyde ... Is Parkinsons Disease a Vesicular Dopamine Storage Disorder? Evidence from a Study in Isolated Synaptic Vesicles of Human and ... Is Parkinsons Disease a Vesicular Dopamine Storage Disorder? Evidence from a Study in Isolated Synaptic Vesicles of Human and ...

2004) Synaptotagmin VII is targeted to dense-core vesicles and regulates their Ca2+ -dependent exocytosis in PC12 cells Journal ... 2008) Two Ca(2+)-dependent steps controlling synaptic vesicle fusion and replenishment at the cerebellar basket cell terminal ... 2005) Synaptotagmin VII is targeted to secretory organelles in PC12 cells, where it functions as a high-affinity calcium sensor ... Cell Biology. Hsf1 and the molecular chaperone Hsp90 support a rewiring stress response leading to an adaptive cell size ...

... cell growth, proliferation, and survival. Less is known regarding its critical role in neuronal physiology, neuronal metabolism ... In PC12 cells, activation of PI3K-C2α enhances the exocytosis of neurosecretory granules, whereas transfection of PC12 cells ... PI3K-C2α is present in clathrin-coated vesicles of isolated brains [105] and binds to clathrin-mediated vesicles, which locally ... Experiments in PC12 cells strongly suggest that PI3K/AKT is the signaling pathway responsible for the activation of late phase ...

Ca2+ Dynamics in the Secretory Vesicles of Neurosecretory PC12 and INS1 Cells  Santo Domingo, Jaime; Fonteriz García, Rosalba ... A confocal study on the visualization of chromaffin cell secretory vesicles with fluorescent targeted probes and acidic dyes  ... Calcium dynamics in bovine adrenal medulla chromaffin cell secretory granules  Santo Domingo, Jaime; Vay, Laura; Camacho, ... Modulation of Ca2+ release and Ca2+ oscillations in HeLa cells and fibroblasts by mitochondrial Ca2+ uniporter stimulation  ...

... cells and will discuss potential functions of vesicle-free miRNAs and how vesicle-free miRNAs regulate cell-to-cell ... then released by vesicle fusion in response to stimulation; in this way, vesicle-free miRNA may regulate cell-to-cell ... then released by vesicle fusion in response to stimulation; in this way vesicle-free miRNA may regulate celltocell ... from neuroendocrine cells and will discuss potential functions of vesicle-free miRNAs and how vesicle-free miRNAs regulate cell ...

Here, we describe a mechanism for dense core vesicle docking in neurons. In Caenorhabditis e ... Docking to the plasma membrane prepares vesicles for rapid release. ... CAPS primes secretory granules in PC12 cells for calcium-stimulated release (Walent et al., 1992; Ann et al., 1997; Grishanin ... For dense core vesicles, the mean number of vesicles or docked vesicles per neuronal profile was calculated by dividing the ...

... by neighboring nerve cells. This release is based on exocytosis: The vesicle docks at the cell membrane, opens at the point of ... They carried out measurements on PC12 cells that release the neurotransmitter dopamine when stimulated by a high potassium ... Tags: B Cell, Cell, Cell Membrane, Cytoplasm, Dopamine, Electrode, Enzyme, G-Protein, Kinase, Living Cells, Mass Spectrometry, ... "By applying an electrode tip to the surface of the cell, we can follow the opening of an individual vesicle and compute the ...

In PC12 cells, Rab11 was detected in association with TGN and TGN-derived secretory vesicles (Urbe, 1993). In baby hamster ... melanosomes of pigment cells, exosomes of maturing red blood cells and secreted vesicles mediating cell-cell signaling. The ... J Extracell Vesicles 11(9): e12263. PubMed ID: 36103151 Summary:. Extracellular vesicle (EV) secretion enables cell-cell ... J Cell Biol 221(6). PubMed ID: 35404399 Summary:. During tissue morphogenesis, the changes in cell shape, resulting from cell- ...

... to ZnO NPs led to a reduction in cell viability and induction of inflammatory response and calcium influx in BV2 and PC12 cells ... This analysis indicated that ZnO NPs can transfer into the CNS, activate glial cells and cause neuroinflammation after tongue ... Moreover, the molecular mechanisms underlying neuroinflammation in vivo were further elucidated by treating BV2 and PC12 cells ... TEM images showed that ZnO NPs were taken up by PC12 cells and located in the vesicles, while numerous vacuoles but not ...

Syt IX localizes to dense core vesicles in PC12 cells. However, in the rat basophilic leukemia (RBL-2H3) mast cell line, as ... Syt IX localizes to dense core vesicles in PC12 cells. However, in the rat basophilic leukemia (RBL-2H3) mast cell line, as ... Syt IX localizes to dense core vesicles in PC12 cells. However, in the rat basophilic leukemia (RBL-2H3) mast cell line, as ... Syt IX localizes to dense core vesicles in PC12 cells. However, in the rat basophilic leukemia (RBL-2H3) mast cell line, as ...

In addition, expression of sialin in PC12 cells did not result in significant vesicle uptake of l-aspartate, and depolarization ... When expressed on the surface of rat hippocampal neurons or PC12 cells, the sensors respond to extracellular glutamate with a ... We acutely purified astrocyte-lineage cells from hCSs at varying stages up to 20 months in vitro using immunopanning and cell ... Synaptic Vesicle Protein NTT4/XT1 (SLC6A17) Catalyzes Na+-coupled Neutral Amino Acid Transport JOURNAL OF BIOLOGICAL CHEMISTRY ...

The small GTPase Cdc42 modulates the number of exocytosis-competent dense-core vesicles in PC12 cells ... modulating both the number of exocytosis-competent dense-core vesicles and the production of dense-core vesicles in PC12 cells ... a pan T cell-specific marker) and quantified the number of cells that were immunoreactive to a T cell antigen receptor, CD3 ( ... form of Cdc42 strongly facilitated high-KCl-induced exocytosis from the newly recruited plasma membrane vesicles in PC12 cells ...

Abrogating Munc18-1 SNARE complex interaction has limited impact on exocytosis in PC12 cells. Malintan, Nancy, Nguyen, Tam H, ... Subdiffractional tracking of internalized molecules reveals heterogeneous motion states of synaptic vesicles. Journal of Cell ... 2 double knockdown reveals the essential functions of interaction between Munc18 and closed syntaxin in PC12 cells. Molecular ... Abrogating Munc18-1 SNARE complex interaction has limited impact on exocytosis in PC12 cells. The Journal of Biological ...

We have developed a cell-free system for regulated exocytosis in the PC12 neuroendocrine cell line. Secretory vesicles were ... A cell-free system for regulated exocytosis in PC12 cells. Avery, J; Ellis, D J; Lang, T; Holroyd, P; Riedel, D; Henderson, R M ... and the cells were sonicated to produce flat, carrier-supported plasma membrane patches with attached vesicles. Exocytosis ... Strain OREX-4, a gram-positive bacterium with a hexagonal S-layer on its cell wall, metabolized compounds commonly used by ...

Using PC12 cells from Rattus norvegicus and artificial supported bilayers, we show that synaptotagmin-1 interacts with the ... This interaction allows both Ca(2+)-binding sites of synaptotagmin-1 to bind to phosphatidylserine in the vesicle membrane upon ... Our results suggest that PIP2 clusters organized by syntaxin-1 act as molecular beacons for vesicle docking, with the ... Synaptic-vesicle exocytosis is mediated by the vesicular Ca(2+) sensor synaptotagmin-1. Synaptotagmin-1 interacts with the ...

Identification of the Alzheimer beta/A4 amyloid precursor protein in clathrin-coated vesicles purified from PC12 cells. J Biol ... Our studies have shown that PKC increases the formation of APP-containing secretory vesicles from the TGN in a cell-free system ... Estrogen can stimulate the formation of APP-containing vesicles from the TGN in cell-free systems derived from both ... There is evidence linking APP to cell adhesion. APP was found to colocalize with β1 intergrins in neural cells [23]. An X-ray ...

... treated PC12 cells. In neuronal cells, TC10 activity at vesicles was higher than its activity at the plasma membrane, and TC10- ... Asymmetric distribution of VANGL2 was faulty in both and chondrocytes indicating impairment of planar cell polarity a ... Large Compact disc163+/Compact disc68+ and Compact disc204+/Compact disc68+ Vorapaxar inhibitor cell ratios had been ... positive vesicles were found to fuse to… Continue reading The use of exocytosis for membrane expansion at nerve growth cones ...

Finally, through Neo-Hookean porohyperelastic (NHPH) material model for the cell, the influence of fluid flow on the aspiration ... Cells experience nonlinear large deformations during the aspiration procedure. Neo-Hookean viscohyperelastic (NHVH) ... role in the deformation behavior of single cells and should be taken into account in the analysis of the mechanics of cells. ... incompressible and compressible models were used to simulate the creep behavior of cells in MA, particularly accounting for the ...

... which α9 integrin and its partner β1 are trafficked along axons and at the growth cone using adult DRG neurons and PC12 cells. ... and immunopurified Rab11 vesicles contain α9 and β1. Endocytosed β1 integrins traffic via Rab11. However, Rab11 vesicles in ... and immunopurified Rab11 vesicles contain α9 and β1. Endocytosed β1 integrins traffic via Rab11. However, Rab11 vesicles in ... and immunopurified Rab11 vesicles contain α9 and β1. Endocytosed β1 integrins traffic via Rab11. However, Rab11 vesicles in ...

These positions will be involved in functional characterization of extracellular vesicles (such as exosomes) in Alzheimers ... Exosomes are cell-derived vesicles that are present in many and perhaps all biological fluids, including blood, urine, and ... Regulation of Dihydropyrimidinase-like 3 Gene Expression by MicroRNAs in PC12 Cells with Induced Ischaemia and Hypothermia ... Extracellular vesicles: biological packages that modulate tumor cell invasion. *Researchers warn - direct-to-consumer ...

... imaging to reveal the diffusional signature for the SNARE proteins Munc18-1 and syntaxin-1A during secretory vesicle priming. ... Single-vesicle trajectories. DKD-PC12 cells were cotransfected with NPY-mCherry and pCMV, untagged Munc18-1WT, or Munc18-1Δ317- ... could rescue the docking of SVs in DKD-PC12 cells. SVs in DKD-PC12 cells lack the ability to dock at the plasma membrane and to ... Cell cultures and transfections. DKD-PC12 cells were maintained as described previously (Han et al., 2009), supplemented with ...

Studies in PC12 cells, a powerful model system, have been especially productive in providing insight into the basic mechanism ... This fusion pore spans both the vesicle and plasma membranes and in many ways behaves like an ion channel. We measure the flux ... Our research focuses broadly on the function of nerve terminals, both how their neurotransmitter-filled vesicles fuse with the ...

In both cell types, the biarsenical staining reagent FlAsH/Lumio Green accumulated in active mitochondria and led to ... We tested the tetracysteine-biarsenical labeling system in live cell fluorescence microscopy of reggie-1/flotillin-2 in HeLa ... which should be considered when this labeling system is to be used in live cell imaging. Mitochondrial accumulation of FlAsH/ ... Recent advances in the field of small molecule labels for live cell imaging promise to overcome some of the limitations set by ...

Therefore, we selected PC12 cells, the widely used cell line with neuronal properties, for further studies. In PC12 cells, PIP3 ... We propose that the GAKIN-PIP3BP complex transports PIP3-containing vesicles from the cell body to the distal end of the ... PIP3BP and GAKIN colocalize at the tip of growing neurites and growth cones of PC12 cells. PC12 cells were transfected with GFP ... PIP3BP and GAKIN colocalize at the tip of growing neurites and growth cones of PC12 cells. PC12 cells were transfected with GFP ...

PC12/IA-2 cells treated with IA-2 siRNA followed by stimulation with low K+, high K+, or high K+ plus PMA decreased dopamine ... as compared to cells treated with control siRNA (Fig. influences the function of dense core vesicles and has a broad modulating ... PC12/IA-2 cells treated with IA-2 siRNA followed by stimulation with low K+, high K+, or high K+ plus PMA decreased dopamine ... By keeping CD8+ T cell alloreactivity out, this CD4+ T cell-restricted model allows us to investigate the reciprocal interplay ...

They are secreted by a range of cell types and via blood reaching other cells whose functioning they can modify because they ... are membranous particles released by cells into the extracellular space. They are involved in cell differentiation, tissue ... focusing on cell-cell communication in physiology and pathology. ... PC12 cells) and in vivo neurons and microglial cells of mouse, ... Extracellular Vesicle-Mediated Cell-Cell Communication in the Nervous System: Focus on Neurological Diseases by Celeste Caruso ...

2 double knockdown reveals the essential functions of interaction between Munc18 and closed syntaxin in PC12 cells. Molecular ... Subdiffractional tracking of internalized molecules reveals heterogeneous motion states of synaptic vesicles. Journal of Cell ... Abrogating Munc18-1 SNARE complex interaction has limited impact on exocytosis in PC12 cells. The Journal of Biological ... Unveiling the nanoscale dynamics of the exocytic machinery in chromaffin cells with single-molecule imaging. Chromaffin Cells ...