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*  Exosomal miR-9 inhibits angiogenesis by targeting MDK and regulating PDK/AKT pathway in nasopharyngeal carcinoma | SpringerLink
Exosomal miRNAs from cancer cells play crucial roles in mediating cell-cell communication and... ... HUVEC cells were cultured in endothelial basal medium (EBM-2) supplemented with 10% fetal bovine serum. NP69 was cultured in ... miR-9 targets CXCR4 and functions as a potential tumor suppressor in nasopharyngeal carcinoma. Carcinogenesis. 2014;35:554-63. ... did not affect the growth of human umbilical vein endothelial cell line (HUVECs) but did enhance endothelial cell migration and ...
  https://link.springer.com/article/10.1186%2Fs13046-018-0814-3
*  Gene Expression Analysis Identifies Potential Biomarkers of Neurofibromatosis Type 1 Including Adrenomedullin | Clinical Cancer...
NF1 is classified as a tumor suppressor gene (2). For example, NF1 patients are at increased risk for developing juvenile ... MPNST cell lines (described in ref. 19) were grown to 90% confluence in DMEM with 10% fetal bovine serum (FBS). Medium was ... Human umbilical vascular endothelial cells (HUVEC) were purchased from Lonza. HUVECs maintained in EBM2 medium (Lonza) with ... Identification of adrenomedullin receptors in cultured rat astrocytes and in neuroblastoma × glioma hybrid cells (NG108-15). ...
  http://clincancerres.aacrjournals.org/content/16/20/5048.long
*  Organs of the immune system
Genes affecting thymus organogenesis and embryonic patterning : gene. fetal expression pattern. phenotype of knockout mice/ ... IRTA1 was mainly expressed by tumor cells involved in lympho-epithelial lesions. In contrast, no or a low number of IRTA1+ ... It also improves and normalizes suppressor cell activities in diabetic patients. Thymosin-b4 can undergo enzymatic cleavage in ... Absent in CNS, PNS, placenta, umbilical cord, cornea and cartilage. In liver, kidney, lungs, heart, pancreas and many endocrine ...
  http://www.ufrgs.br/imunovet/molecular_immunology/organsimmune.html
*  AAV-mediated human PEDF inhibits tumor growth and metastasis in murine colorectal peritoneal carcinomatosis model | BMC Cancer ...
This result suggested that rAAV2-hPEDF could transfer hPEDF gene into cultured cells and produce secretory protein. ... fetal bovine serum (FBS, GIBCO, USA) and 1% penicillin/streptomycin. Primary HUVECs were isolated from human umbilical cord ... as a potent tumor suppressor and a potential candidate for cancer gene therapy. ... Induction of tumor apoptosis. To explore the role of rAAV2-hPEDF on apoptosis of tumor cells, tumor resections were subjected ...
  https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-12-129
*  Patente WO2011099007A1 - Pharmaceutical compositions and methods for the treatment and prevention of ... - Google Patentes
These cell extracts can direct epigenetic reprogramming of the cancer cells and thereby reverse their neoplastic phenotype. ... or decrease cell transformation. Tumor suppressor genes are genes that encode protein(s) that regulate cell growth and ... Cells are cultured in alpha-MEM medium supplemented with 10% fetal bovine serum and 100 units/ml penicillin/streptomycin at ... Isolation of stem/progenitor cells from amniotic membrane of umbilical cord.. WO2008014426A2. 26 Jul 2007. 31 Ene 2008. ...
  http://www.google.es/patents/WO2011099007A1?cl=en
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Using the BLT Humanized Mouse as a Stem Cell based Gene Therapy Tumor Model ... Immunology, Issue 89, Varicella zoster virus, cell-mediated immunity, T cells, interferon gamma, ELISpot, umbilical cord blood ... resulting in expression of mutated oncogenes and deletion of tumor suppressor genes. The phenotypic consequences of these ... The earlier models involved the use of C. B 17 scid/scid mice and the transplantation of human fetal thymus and fetal liver ...
  https://www.jove.com/visualize/abstract/21085478/cost-effectiveness-primary-prophylaxis-aids-associated-cryptococcosis
*  Articles Added on November 17, 2012
Cell cycle gene-specific control of transcription has a critical role in proliferation ... ... Gastrokines: stomach-specific proteins with putative homeostatic and tumor suppressor roles. Menheniott Trevelyan R - - 2012 ... Fetal intrauterine growth restriction (IUGR) results in increased placental resistance to blood flow, fetal hypertension, and ... extract of sappanwood was found to inhibit melanin synthesis in cultured human melanoma HMV-II cells stimulated with forskolin ...
  http://www.biomedsearch.com/added/20121117-p10.html
*  Autophagy and Stem Cells | SpringerLink
Beclin 1, an autophagy gene essential for early embryonic development, is a haploinsufficient tumor suppressor. Proc Natl Acad ... FIP200 is required for the cell-autonomous maintenance of fetal hematopoietic stem cells. Blood. 2010;116(23):4806.CrossRef ... Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. ... Autophagy is required for human umbilical cord mesenchymal stem cells to improve spatial working memory in APP/PS1 transgenic ...
  https://link.springer.com/chapter/10.1007%2F978-3-319-98146-8_1
*  IJMS | Free Full-Text | Wharton's Jelly-Derived Mesenchymal Stem Cells: Phenotypic Characterization and Optimizing Their ...
... stemness and immune properties appear to be more robustly expressed and functional which are more comparable with fetal than ... A unique cell population of WJ that has been suggested as displaying the stemness phenotype is the mesenchymal stromal cells ( ... is a gelatinous tissue within the umbilical cord that contains myofibroblast-like stromal cells. ... microarray analysis of rat UCMSC revealed over-expression of multiple tumor suppressor gene [65]. The second potential ...
  http://mdpi.com/1422-0067/14/6/11692/htm
*  PEDF - Wikipedia
PEDF induces PPAR-gamma expression which in turn induces p53, a tumor suppressor gene involved in cell cycle regulation and ... to chromosome 17p13.1 and expression in cultured human retinoblastoma cells". Genomics. 19 (2): 266-72. doi:10.1006/geno. ... "PEDF induces p53-mediated apoptosis through PPAR gamma signaling in human umbilical vein endothelial cells". Cardiovascular ... Soon thereafter, the same laboratory sequenced the PEDF protein and compared it to a human fetal eye library. They found that ...
  https://en.wikipedia.org/wiki/PEDF
*  Hyaluronic acid - Wikipedia
By producing anti-adhesive HA, HAS can allow tumor cells to release from the primary tumor mass, and if HA associates with ... During fetal development, the migration path through which neural crest cells migrate is rich in HA. HA is closely associated ... Yoshihara S, Kon A, Kudo D, Nakazawa H, Kakizaki I, Sasaki M, Endo M, Takagaki K (2005). "A hyaluronan synthase suppressor, 4- ... The average molecular weight in human synovial fluid is 3-4 million Da, and hyaluronic acid purified from human umbilical cord ...
  https://en.wikipedia.org/wiki/Hyaluronic_acid
*  Protocols and Video Articles Authored by David Warburton (Translated to Portuguese)
Dec, 2011 , Pubmed ID: 22167068 Even though the role of the tyrosine phosphatase PTEN as a tumor suppressor gene has been well ... In the case of CF, the Cftr gene has been shown to regulate fetal lung epithelial cell differentiation and maturation. Subtle ... When cultured AEC2 placed in suspension were labeled with fluorescent-tagged antibodies to E-cadherin, cells could be sorted ... on human umbilical vascular endothelial cells (HUVECs). HUVECs were treated either directly with LeTx or indirectly with LeTx ...
  https://www.jove.com/author/David_Warburton?language=Portuguese
*  Protocols and Video Articles Authored by Peng Jin (Translated to Danish)
We recovered 19 mutations in the tumor suppressor lethal (2) giant larvae (dlgl) gene and 90 mutations at other loci. dlgl ... In cultured COLO205 cells, the effect of estradiol (E2) and antagonist ICI182.780 on the expression of hMLH1 and hMSH2 was ... Tei Index for Prenatal Diagnosis of Acute Fetal Hypoxia Due to Intermittent Umbilical Cord Occlusion in an Animal Model ... In order to screen out important genes from large gene data of gene microarray after nerve injury, we combine gene ontology (GO ...
  https://www.jove.com/author/Peng_Jin?language=Danish
*  The RNA binding protein SORBS2 suppresses metastatic colonization of ovarian cancer by stabilizing tumor-suppressive...
SORBS2 suppresses ovarian cancer invasiveness and affects monocyte to myeloid-derived suppressor cell and M2-like macrophage ... By enhancing the stability of these gene transcripts, ... is a potent suppressor of ovarian cancer metastatic ... Our data illustrate a novel post-transcriptional network that links cancer progression and immunomodulation within the tumor ... and COV504 were cultured in Dulbecco's modified Eagle medium supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine, ...
  https://genomebiology.biomedcentral.com/articles/10.1186/s13059-018-1412-6
*  Anti-angiogenic potential of an ethanol extract of Annona atemoya seeds in vitro and in vivo | BMC Complementary and...
... and tube formation by human umbilical vascular endothelial cells (HUVECs); a Matrigel plug assay; and tumor-induced ... The anti-angiogenic potential of EEAA was evaluated using various in vitro/in vivo models, including cell proliferation, ... in cancer cells under hypoxic conditions. EEAA shows a strong anti-angiogenic potential in both in vitro and in vivo systems, ... plays a pivotal role during tumor development and metastasis. Therefore, many efforts have been made to develop effective anti- ...
  https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-14-353
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
8 Most anti-tumor gene therapies are focused on administration of the tumor suppressor genes, such as wild-type p53 (wt-p53), ... fetal organs have an increased cell proliferation rate as compared to adult organs, which could allow a more efficient gene or ... The arisen metastases are isolated and cultured in vitro as cell lines, which often show enhanced metastatic abilities than the ... 16 or umbilical vein17. Our novel surgical procedure enables researchers to inject the agent of choice directly into the fetal ...
  https://www.jove.com/visualize/abstract/23409158/the-relationship-between-xrcc1-xrcc3-gene-polymorphisms-lung-cancer
*  Inhibition of Endothelial NOTCH1 Signaling Attenuates Inflammation by Reducing Cytokine-Mediated Histone Acetylation at...
A, Human umbilical vein endothelial cells (HUVECs) were treated with IL (interleukin)-1β (1 ng/mL), TNF (tumor necrosis factor ... were isolated as described previously19 and cultured on 0.1% gelatin-coated plastic in MCBD 131 medium containing 7.5% fetal ... A-C, Gene set enrichment analysis of IL (interleukin)-1β-stimulated Human umbilical vein endothelial cells (HUVECs) comparing ... suppressor of hairless], or CSL [CBF1/RBP-Jκ, Su(H), Lag1]).1,2 ... human umbilical vein endothelial cells. IL. interleukin. JAG. ...
  http://atvb.ahajournals.org/content/38/4/854
*  The Light and Shadow of Senescence and Inflammation in Cardiovascular Pathology and Regenerative Medicine
... biological response modifiers and the family of cell adhesion-promoting molecules. ... to downregulate tumor suppressor RB. Consequently, the vascular cell is arrested at the Gap 1 phase of the mitotic replication ... the transcription factor gene T-box 1, and the fibroblast growth factor genes Fgf-8/10. Other Isl-1 cells, coexpressing the ... and fetal-type mesenchymal stem cells in a rat model of heart cryoinjury," Cell Transplantation, vol. 17, pp. 679-694, 2008. ...
  https://www.hindawi.com/journals/mi/2017/7953486/
*  MPGES-1 - Wikipedia
"Interleukin 1beta induces functional prostaglandin E synthase in cultured human umbilical vein endothelial cells". J. Physiol. ... Its expression can also be induced by tumor suppressor protein TP53, and may be involved in TP53-induced apoptosis. Knockout ... The protein encoded by this gene is a glutathione-dependent prostaglandin E synthase. The expression of this gene has been ... Alfaidy N, Sun M, Challis JR, Gibb W (2003). "Expression of membrane prostaglandin E synthase in human placenta and fetal ...
  https://en.wikipedia.org/wiki/MPGES-1
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... resulting in expression of mutated oncogenes and deletion of tumor suppressor genes. The phenotypic consequences of these ... and human umbilical vein endothelial cells (HUVEC), but are applicable to all adherent growing cells. ... It utilizes pressure applied to the cells cultured in flexible-bottomed wells. The pressure applied may easily be controlled ... Medicine, Issue 89, fetal, ethanol, exposure, paradigm, vapor, development, alcoholism, teratogenic, animal, mouse, model ...
  https://www.jove.com/visualize/abstract/22675443/transcriptional-profiling-human-brain-endothelial-cells-reveals-key
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... resulting in expression of mutated oncogenes and deletion of tumor suppressor genes. The phenotypic consequences of these ... The umbilical cord is a rich source for progenitor cells with high proliferative potential including mesenchymal stromal cells ... spheroid cultures that maintain constant cell/cell contact. The chopping method has primarily been used for fetal brain-derived ... Neuroscience, Issue 79, Neurobiology, Cellular Biology, Cells, Cultured, Neurons, Central Nervous System, Neurodegenerative ...
  https://www.jove.com/visualize/abstract/25115920/mesenchymal-stromal-cell-proliferation-gene-expression-protein
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... fetal organs have an increased cell proliferation rate as compared to adult organs, which could allow a more efficient gene or ... resulting in expression of mutated oncogenes and deletion of tumor suppressor genes. The phenotypic consequences of these ... When cultured on OP9 stromal cells, ES cells differentiate into Flk-1+ hemangioblasts, hematopoietic progenitors, and finally ... 16 or umbilical vein17. Our novel surgical procedure enables researchers to inject the agent of choice directly into the fetal ...
  https://www.jove.com/visualize/abstract/21931777/epstein-barr-virus-induced-gene-3-ebi3-novel-diagnosis-marker-burkitt
*  Application # 2011/0230544. MODULATION OF C-REACTIVE PROTEIN EXPRESSION - Patents.com
HMECs and MCF7 cells express p53, whereas T47D cells do not express this tumor suppressor gene. Cells were cultured as ... fetal bovine serum (Invitrogen Life Technologies, Carlsbad, Calif.). T47D cells were cultured in DMEM High glucose media ( ... examined in human umbilical vascular endothelial cells (HUVECs) (ATCC, Manassus, Va.). HUVECs are cultured according to the ... The latter two cell lines express similar genes. MCF7 cells express the tumor suppressor p53, while T47D cells are deficient in ...
  http://patents.com/us-20110230544.html
*  EZH2-dependent chromatin looping controls INK4a and INK4b, but not ARF, during human progenitor cell differentiation and...
During progenitor cell differentiation and ageing, PcG silencer EZH2 attenuates, causing loss of PRC binding and ... We found that INK4b and INK4a, but not ARF, are upregulated following the differentiation of haematopoietic progenitor cells, ... coupling human progenitor cell differentiation to proliferation control. Our results revealed a chromatin looping mechanism of ... in ageing fibroblasts and in senescing malignant rhabdoid tumour cells. To investigate the underlying molecular mechanism we ...
  https://epigeneticsandchromatin.biomedcentral.com/articles/10.1186/1756-8935-2-16
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... for the genes of interest. The cells were subsequently cultured in medium containing growth factors other than erythropoietin ( ... 16 or umbilical vein17. Our novel surgical procedure enables researchers to inject the agent of choice directly into the fetal ... Thermal tumor ablation can be performed at open surgery, laparoscopy or using a percutaneous approach. When performed ... This protocol can be implemented for novel future haploid screens, such as in enhancer and suppressor screens, to address the ...
  https://www.jove.com/visualize/abstract/25923652/fetal-calcifications-are-associated-with-chromosomal-abnormalities