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*  Difference between revisions of "User:Pranav Rathi/Notebook/OT/2012/11/13/Optical Tweezers Calibration" - OpenWetWare
It is absolutely unpractical to measure the laser power in the trap before every power-spectrum data is acquired to calculate ... The parameters I want to calibrate: Stiffness; which measures the force that is exerts on the trapped bead, and Sensitivity; ... I put a water droplet on the objective and record the power with a power meter directly. I used Thorlabs sensor: model D10MM ( ... Difference between revisions of "User:Pranav Rathi/Notebook/OT/2012/11/13/Optical Tweezers Calibration". From OpenWetWare ...
  https://openwetware.org/wiki/?title=User:Pranav_Rathi/Notebook/OT/2012/11/13/Optical_Tweezers_Calibration&curid=126877&diff=657417&oldid=657414
*  Live cell imaging of mechanotransduction | Journal of The Royal Society Interface
Optical tweezers use lights to manipulate microscopic objects. The radiation pressure from a focused laser beam can trap and ... A force-sensing lever arm based on the silicon micro-electro-mechanical system technology was developed to exert and measure ... When the tip of the force sensor is inserted into the droplet of FN solution to suck the liquid and result in stretching the FN ... Relatively local stretch can be conveniently applied on cells with laser/optical tweezers or magnetic tweezers. ...
  http://rsif.royalsocietypublishing.org/content/early/2010/03/29/rsif.2010.0042.focus
*  Frontiers | Haptic Feedback for Microrobotics Applications: A Review | Robotics and AI
To overcome the lack of force-sensing, vision seems a promising solution. Finally, although the literature on haptic feedback ... To overcome the lack of force-sensing, vision se... ... Another challenge lies in the measurement of position and force ... Another challenge lies in the measurement of position and force signals in the remote environment. The integration of ... "First high speed simultaneous force feedback for multi-trap optical tweezers," in Advanced Intelligent Mechatronics (AIM), 2014 ...
  https://www.frontiersin.org/articles/10.3389/frobt.2016.00053/full
*  Difference between revisions of "User:Pranav Rathi/Notebook/OT/2012/11/13/Optical Tweezers Calibration" - OpenWetWare
We can assume that the trap exerts a linear spring force on the bead. The one-dimensional equation of motion of a trapped bead ... It is absolutely unpractical to measure the laser power in the trap before every power-spectrum data is acquired to calculate ... I put a water droplet on the objective and record the power with a power meter directly. I used Thorlabs sensor: model D10MM ( ... Optical tweezers calibration schematics in image shows all the parameters and programs I use. To determine the force exerted by ...
  https://openwetware.org/wiki/?title=User:Pranav_Rathi/Notebook/OT/2012/11/13/Optical_Tweezers_Calibration&diff=658487&oldid=658472
*  Single-cell nanosurgery | Mo Costandi
... involves the use of laser beams focused by an objective microscope lens to exert a controlled force onto, and therefore ... whereas mitochondria trapped in optical tweezers were bleached within 2 seconds, those trapped in an optical vortex remained ... Since then, the technique has been used to investigate the mechanical properties of microtubules and to measure the forces ... In particular, we are developing a droplet nanolab platform for single-organelle assays and chemical analysis, because many ...
  https://neurophilosophy.wordpress.com/2007/01/16/single-cell-nanosurgery/
*  Open access articles from Soft Matter Home
Using Brownian motion to measure shape asymmetry in mesoscopic matter using optical tweezers Basudev Roy, Argha Mondal, Sudipta ... The bending modulus κ is known to be a crucial parameter for the stability of the droplet phase. By combination of droplet ... Multifrequency atomic force microscopy is used to gain sub-nanometre insights into the structure of aquaporin 0 and its ... The apparent charge of nanoparticles trapped at a water interface Guilherme Volpe Bossa, Joseph Roth, Klemen Bohinc and Sylvio ...
  http://pubs.rsc.org/en/journals/articlecollectionlanding?sercode=sm&themeid=e297b895-0853-410c-8079-aa5917ba7475
*  Blog on Optical Tweezers: November 2011
We measured the optical force constants using linearly and radially polarized trapping beams finding a reshaping of the optical ... the cell aligns along the optical axis. A defocused-edge detection method was devised to measure the orientation of the rotated ... Mechanical stochastic tug-of-war models cannot explain bidirectional lipid-droplet transport ... We used optical tweezers to rotate bacterial cells relative to the optical axis. We rapidly oscillate the optical tweezers ...
  http://opticaltweezers.blogspot.com/2011_11_01_archive.html
*  Microfluidic tools for cell biological research
... optical tweezers [116], or complex dedicated force machines [117]. Most of these apparatus measure global and local forces of ... Picture of a cell trapping array using hydrodynamic trap and zoom on a single cell trap [168]. (B) Droplet based yeast culture ... measured the force produced by a bacteria film and a single fungus appressoria using a PDMS membrane with optical deformation ... Methods previously mentioned cannot measure side forces. However, it is possible to measure side forces using pillars or thin ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC2998071/
*  Laser manipulation in liquid crystals: an approach to microfluidics and micromachines | Philosophical Transactions of the Royal...
... drag force or a stronger optical trap. The anisotropy of the optical trapping forces is considered to be low owing to the steep ... You described the use of optical tweezers to drag a colloidal particle through a nematic liquid and measure from the velocity ... thus decreasing the axial trapping force that allows the droplet to be trapped in three dimensions. This is readily studied ... An established method for measuring optical trapping forces is to apply a known viscous drag force to the optically trapped ...
  http://rsta.royalsocietypublishing.org/content/364/1847/2789
*  New England Complex Fluids Workgroup
One experimental approach to active microrheology uses optical tweezers, which trap a μm-sized particle located within the ... "Traction Force Rheology of Colloidal Solids". We present a new technique, traction force rheology, to directly measure the ... We have developed a droplet-based microfluidics technology that is capable of first encapsulating single cells in droplet, ... which is a direct measure of the viscoelastic response of the material.. Keywords: Microrheology, Biopolymer, Optical tweezers ...
  http://www.complexfluids.org/necf/abstracts.php?meetingID=75
*  Blog on Optical Tweezers: May 2012
We measure the fluctuations of the position of a Brownian particle confined by an optical trap in an aging gelatin droplet ... We report optical tweezers with the assistance of a strong instantaneous force generated by a focused pulse laser beam of sub- ... Optical Fiber Technology (1) Optical Materials (1) Optical Materials Express (4) Optical Memory and Neural Networks (1) Optical ... Blog on Optical Tweezers Concisely bringing the latest news and relevant information regarding optical trapping and ...
  http://opticaltweezers.blogspot.com/2012/05/
*  Blog on Optical Tweezers: January 2011
We explain the flake dynamics in the optical trap and measure force and torque constants from the correlation functions of the ... a nematic droplet held in laser tweezers. The identification and spatial evolution of such a topological fingerprint is ... Optical Fiber Technology (1) Optical Materials (1) Optical Materials Express (3) Optical Memory and Neural Networks (1) Optical ... Blog on Optical Tweezers Concisely bringing the latest news and relevant information regarding optical trapping and ...
  http://opticaltweezers.blogspot.com/2011_01_01_archive.html
*  Blog on Optical Tweezers: February 2010
We used optical tweezers to measure the rupture force between HU proteins and Lon; HUα, HUβ, and HUβ-A20D can bind to Lon, and ... Side imaging was used in conjunction with imaging in the plane of the optical trap. Droplet sizing was performed using cavity- ... Optical fibre probe-based near-field optical tweezers overcomes the diffraction limit of conventional optical tweezers, ... Optical Fiber Technology (1) Optical Materials (1) Optical Materials Express (4) Optical Memory and Neural Networks (1) Optical ...
  http://opticaltweezers.blogspot.com/2010/02/
*  Blog on Optical Tweezers: June 2010
In addition, by integrating the optical force potential with the temperature elevation under optical trapping condition, it was ... manipulated with an optical trap, and a streptavidin-functionalized coverslip were used to measure the effect of different ... Here, the nanoparticles covered the entire area of the assembled SnO2 rod by evaporating a droplet of nanoparticle solution ... Optical Fiber Technology (1) Optical Materials (1) Optical Materials Express (3) Optical Memory and Neural Networks (1) Optical ...
  http://opticaltweezers.blogspot.com/2010_06_01_archive.html
*  Blog on Optical Tweezers: April 2012
The end-to-end distance of DNA molecules was varied by using dual trap optical tweezers, and the force-fluctuation relation was ... to calibrate optical tweezers by using only videomicroscopy to measure the position autocorrelation function of the trapped ... Further, an increase in core intensity was shown to broaden the range in droplet sizes that were optically trapped. The ... Optical Fiber Technology (1) Optical Materials (1) Optical Materials Express (3) Optical Memory and Neural Networks (1) Optical ...
  http://opticaltweezers.blogspot.com/2012_04_01_archive.html
*  Blog on Optical Tweezers: January 2010
... optical trap, where the trapped object has an active role in enhancing the restoring force. We demonstrate experimentally ... The ability of the sensor to measure the dynamic position and the forces between six optically trapped spheres, down to ... If the object is relatively easy to deform, as is, e.g., a low surface tension droplet, the resulting deformation is easily ... Optical Fiber Technology (1) Optical Materials (1) Optical Materials Express (4) Optical Memory and Neural Networks (1) Optical ...
  http://opticaltweezers.blogspot.com/2010/01/
*  Blog on Optical Tweezers: November 2009
Moving an optical trap in response to the trapped object's motion is a powerful technique for optical control and force ... By analyzing the motion correlations of the trapped nanostructures, we measure with high accuracy both the optical trapping ... The optical properties of the droplet are determined using stimulated Raman scattering. The droplet is in thermodynamic ... we created multiple optical traps with an inclined dual-fiber optical tweezers setup. One 3D trap and two 2D traps were formed ...
  http://opticaltweezers.blogspot.com/2009_11_01_archive.html
*  Publications | Stavans Lab
This technique is demonstrated by applying it to a single Brownian particle in an optical trap of force constant k. The decay ... this approach by measuring the three-dimensional force constants of a single bead and a pair of beads trapped by laser tweezers ... By scattering a focused laser beam from a particle observed in an optical microscope, we measure its fluctuations via the ... Phase diagrams of droplet volume fraction versus polymer concentration are presented. At large droplet volume fractions where ...
  http://www.weizmann.ac.il/complex/stavans/publications
*  Blog on Optical Tweezers: September 2010
Droplet manipulation and trapping potential in such a two-dimensional anisotropic system show that optical trapping has two ... Random Brownian force affects the trajectories. Meanwhile, trapping potential depths and minimum trapping particle radii in ... By combining experimental data with a physical model and an optimization algorithm for analysis, we measure the 10 unique ... Optical Fiber Technology (1) Optical Materials (1) Optical Materials Express (3) Optical Memory and Neural Networks (1) Optical ...
  http://opticaltweezers.blogspot.com/2010_09_01_archive.html
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
These techniques include so-called force spectroscopy approaches such as atomic force microscopy, optical tweezers, flow ... magnetic tweezers and the freely-orbiting magnetic tweezers, which makes it possible to directly measure the torque stored in a ... In this assay, neutrophils migrate directly from the blood droplet, through small channels, towards the source of ... side chains from the AM form of the Ca2+ indicator and a hydrophilic fluorescent dye/Ca2+ complex is formed and trapped in the ...
  https://www.jove.com/visualize/abstract/19956649/systematic-differences-in-impact-across-publication-tracks-at-pnas
*  Patente US20110229872 - Microfabricated Cell Sorter - Google Patentes
... can be sorted into an appropriate branch channel based on the presence or amount of a detectable signal such as an optical ... Optical Trapping. * [0175] In another embodiment, an optical trap, or laser tweezers, may be used to sort or direct cells in a ... A charged droplet may cause the next droplet of the opposite polarity to have a reduced charge. On the other hand, if ... The capillary force causes the sample to enter the device. The force and direction of flow can be controlled by any desired ...
  http://www.google.es/patents/US20110229872
*  Blog on Optical Tweezers: October 2009
We present measurements that characterize the mirror traps in terms of trap stiffness, maximum trapping force and capture range ... We applied oscillatory optical tweezers to measure the differential stiffness of dsDNA molecules, interacting with RecA, as a ... 2) Positive/negative photophoresis on a droplet: We show that a droplet is driven by a laser beam, either toward and backward ... Optical Fiber Technology (1) Optical Materials (1) Optical Materials Express (3) Optical Memory and Neural Networks (1) Optical ...
  http://opticaltweezers.blogspot.com/2009_10_01_archive.html
*  Blog on Optical Tweezers: September 2009
Optical trapping experiments were used to measure directly the mechanical properties of these spirochetes. These results imply ... The force field of optical tweezers is commonly assumed to be conservative, neglecting the complex action of the scattering ... isolation we compare the influence of different optical landscapes on the flow of free aerosol around a trapped droplet. We ... Optical Fiber Technology (1) Optical Materials (1) Optical Materials Express (3) Optical Memory and Neural Networks (1) Optical ...
  http://opticaltweezers.blogspot.com/2009_09_01_archive.html
*  Download Alien Encounters
Prerequisite of practice; transition tweezers findings in new military reflection projects. download of application; optical ... groups, download, force, fires, and basis of class permissions. May See been for download alien when records 'm. draws cross: ... now 13 queue in link( more on the droplet). lost from and been by Amazon. Please call a sexual UK gene. Please become a ... One download alien of such a life is a range; plugin; tools; robot; that, when trapped, widely actually is many man embedded on ...
  http://pd19.org/ebook/download-alien-encounters
*  DEVICE AND METHOD FOR PRESSURE-DRIVEN PLUG TRANSPORT - Patent application
0409] The plug traps are separated by narrow regions that help force the plugs into the traps. [0410] In this experiment, a ... optical tweezers, and combinations thereof, without regard for any particular theory or mechanism of action. [0089] The term " ... an optical microscope. Long exposures (i.e., about 2 seconds) have been used to measure fast (i.e., about 2 milliseconds) ... Vapor Diffusion Protein Crystallization in Capillaries by an Alternating Droplet System [0429] The principle of transferring ...
  http://www.patentsencyclopedia.com/app/20110177586