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*  Patent US5668255 - Hybrid molecules having translocation region and cell-binding region - Google Patents
Pseudomonas exotoxin A, cholera toxin, ricin toxin, and Shiga-like toxin, is capable of translocating such effector region ... the portion of the translocation domain, when covalently bonded to the enzymatically-active effector region of a toxin selected ... the second part including a portion of a translocation domain of a protein, provided that (i) the hybrid molecule does not ... the first part including a portion of the binding domain of a cell-binding ligand, which portion is able to cause the hybrid ...
  http://www.google.com.au/patents/US5668255
*  Diphtheria toxin - Wikipedia
Fragment A contains the catalytic C domain, and fragment B consists of the T and R domains: The amino-terminal catalytic domain ... Diphtheria toxin is an exotoxin secreted by Corynebacterium diphtheriae, the pathogenic bacterium that causes diphtheria. ... A central translocation domain, known as the T domain or TM domain, has a multi-helical globin-like fold with two additional ... Myocarditis secondary to diphtheria toxin is considered one of the biggest risks to unimmunized children. Diphtheria toxin was ...
  https://en.wikipedia.org/wiki/Diphtheria_toxin
*  PYRROLOPYRAZINONE INHIBITORS OF KINASES - Patent application
... catalytic and translocation domains of diphtheria toxin fused via a His-Ala linker to human epidermal growth factor) or ... IL-13-pseudomonas exotoxin, interferon-α, interferon-γ, JUNOVAN® or MEPACT® (mifamurtide), lonafarnib, 5,10- ... Each binding site comprises a heavy chain variable domain and a light chain variable domain with a total of 6 CDRs involved in ... Dual variable domain (DVD) binding proteins are tetravalent or multivalent binding proteins binding proteins comprising two or ...
  http://www.patentsencyclopedia.com/app/20110257189
*  Solid dispersions containing apoptosis promoting proteins
... catalytic and translocation domains of diphtheria toxin fused via a linker His-Ala with epidermal growth factor), dacarbazine, ... IL-13-exotoxinPseudomonas, interferon-α, interferon-γ, Junovan™ and Mepact™ (mifamurtide), lonafarnib, 5,10- ... Each binding site contains a variable domain of the heavy chain variable domain and light chain total of 6 CDRs involved in ... diphtheria toxins), tretinoin (retina-A), Trisenox™ (arsenic trioxide), Ukrain™ (derivative of alkaloids from plants celandine ...
  http://russianpatents.com/patent/255/2550134.html
*  Extracellular Bad Fused to Toxin Transport Domains Induces Apoptosis | Cancer Research
4 The abbreviations used are: DT, diphtheria toxin; DTTR, translocation and receptor-binding domains of DT; STS, staurosporine ... Alternatively, bacterial toxins such as DT, 4 pseudomonas exotoxin, and anthrax toxin that reach the cytosol by translocation ... translocation to the cytosol, and then catalytic inactivation of cellular proteins, leading to cell death (31) . The catalytic ... we genetically fused the mouse Bad gene to the gene for the translocation and receptor-binding domains of diphtheria toxin ( ...
  http://cancerres.aacrjournals.org/content/62/5/1433
*  ADP-ribosylation - Wikipedia
Exotoxin A of Pseudomonas aeruginosa; Pertussis toxin of B. Pertussis; C3 toxin of C. botulinum; and Diphtheria toxin of ... domain for translocation of the enzyme across the membrane of the cell. These domains can exist in concert in three forms: ... PARPs use a catalytic triad of His-Tyr-Glu to facilitate binding of NAD+ and positioning of the end of the existing poly-ADP ... It is also the basis for the toxicity of bacterial compounds such as cholera toxin, diphtheria toxin, and others. The first ...
  https://en.wikipedia.org/wiki/ADP-ribosylation
*  Antibodies | Free Full-Text | Diving through Membranes: Molecular Cunning to Enforce the Endosomal Escape of Antibody-Targeted...
Thus, enforcing the endosomal escape of anti-tumor toxins without affecting the integrity of other cellular membranes is of ... the endosomal escape of targeted toxins include the use of the translocation motif of diphtheria toxin or Pseudomonas exotoxin ... that binds the cell surface receptor and initiates translocation of the catalytic protein or other protein domain (marked as A ... diphtheria toxin and Pseudomonas exotoxin A are administered in a truncated form with the cell binding domain deleted and the ...
  http://mdpi.com/2073-4468/2/2/209/htm
*  Recombinant Immunotoxin Therapy of Glioblastoma: Smart Design, Key Findings, and Specific Challenges
Solution and membrane-bound chaperone activity of the diphtheria toxin translocation domain towards the catalytic domain. FEBS ... the engineered toxin components in the majority of the RITs are from either Pseudomonas exotoxin A (PE) or diphtheria toxin (DT ... T domain) that helps with translocation of A chain into cytosol; and the other is the catalytic domain (C domain) that exerts ... AB toxins possess three functional domains: one is the receptor binding domain (R domain) that enables toxin to be absorbed on ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC5511670/
*  Frontiers | mADP-RTs: versatile virulence factors from bacterial pathogens of plants and mammals | Plant Science
... pertussis toxin and diphteria toxin are three of the best-known examples of mADP-RTs. They modify host target proteins in order ... Despite low sequence similarity at the primary amino acid level, mADP-RTs share a conserved core catalytic fold and structural ... pertussis toxin and diphteria toxin are three of the best-known examples of mADP-RTs. They modify host target proteins in order ... Despite low sequence similarity at the primary amino acid level, mADP-RTs share a conserved core catalytic fold and structural ...
  https://www.frontiersin.org/articles/10.3389/fpls.2012.00142/full
*  David B. McKay | Stanford Medicine Profiles
Exotoxin A of Pseudomonas aeruginosa is a secreted bacterial toxin capable of translocating a catalytic domain into mammalian ... X-RAY GRADE CRYSTALS OF DIPHTHERIA-TOXIN JOURNAL OF BIOLOGICAL CHEMISTRY Collier, R. J., Westbrook, E. M., McKay, D. B., ... The other two domains are presumably involved in cell receptor binding and membrane translocation. ... to that of the enzymatic domain alone reveals a salt bridge between Arg467 of the catalytic domain and Glu348 of domain II that ...
  https://med.stanford.edu/profiles/david-mckay
*  Indian Patents. 253564:'METHOD FOR EXPRESSING AN IMMUNOTOXIN IN PICHIA PASTORIS'
The cytosolic entry of diphtheria toxin catalytic domain requires a host cell cytosolic translocation factor complex. J Cell ... Toxins that may be used include but are not limited to diphtheria toxin, ricin toxin, and pseudomonas exotoxin. The antibody ... Woo et al., 2002). However, diphtheria toxin (DT) is a very potent toxin to all eukaryotic cells if its catalytic domain can ... wherein the immunotoxin comprises a diphtheria toxin moiety.. 7. The method as claimed in claim 6, wherein the diphtheria toxin ...
  http://www.allindianpatents.com/patents/253564-method-for-expressing-an-immunotoxin-in-pichia-pastoris
*  Frontiers | Targeting Myelogenous Leukemia Stem Cells: Role of the Circulation | Oncology
CD123 has been targeted for clinical use with a diphtheria toxin-IL-3 fusion protein (Kuo et al., 2009) and numerous additional ... Inhibition of the mTOR catalytic site with OSI-027, a dual mTORC1/mTORC2 inhibitor, resulted in suppression of mTORC2 and ... 2005), using intravital microscopy showed that NALM-6, an ALL cell line, engrafted in microvascular domains in the marrow. ... This binding created two cell death inducing molecules; one being immunotoxic through truncation with a pseudomonas exotoxin ...
  https://www.frontiersin.org/articles/10.3389/fonc.2012.00086/full
*  FUSION PROTEINS - Patent application
TABLE-US-00006 Translocation Amino acid domain source residues References Diphtheria toxin 194-380 Silverman et al., 1994, J. ... alia by plant toxins such as ricin, and abrin, and by bacterial toxins such as diphtheria toxin, and Pseudomonas exotoxin A. ... 6 In vitro catalytic activity of a LC/A-nociceptin-HN/A fusion protein [0154] FIG. 7 Purification of a LC/A-nociceptin variant- ... The translocation domain can be the natural translocation domain of the toxin or it can be a different translocation domain ...
  http://www.patentsencyclopedia.com/app/20120064059
*  EPCAM APTAMER FOR DETECTION OF CANCER STEM CELLS - DEAKIN UNIVERSITY
The natural diphtheria toxin molecule secreted by Corynebacterium diptheriae consist of several functional domains that can be ... LT toxin, C3 toxin, Shiga toxin pertussis toxin, tetanus toxin, Pseudomonas exotoxin, alorin, saponin, modeccin and gelanin. ... which includes a translocation domain and a generalised cell binding domain (AA 475-535). ... catalytic cores, sites for selective binding to affinity columns, and other sequences to facilitate cloning and/or sequencing ...
  http://www.freepatentsonline.com/y2015/0197755.html
*  2005, November - Journal of Young Investigators
In fact, protective antibody titers usually decrease over time (example: diphtheria, tetanus toxins, and measles vaccines). All ... likely by affecting toxin trafficking into the cell. The single A subunit is comprised of two major structural domains termed ... Several vaccines are made from the B subunits without the catalytic subunit, ensuring no toxin activity. The B subunits bind to ... After membrane translocation, the A1 peptide may move to the adenylyl cyclase complex on the cytoplasmic surface of the ...
  https://www.jyi.org/2005-november
*  Search Results - - 57 Results - Digital Library
In contrast, Diphtheria toxin, Pseudomonas exotoxin A, and eukaryotic poly-ADP-ribosyltransferases are characterized by ... conserved Arg and catalytic Glu residues. The NAD-binding core of a binary toxin and a C3-like toxin family identified an ARTT ... The TON2 protein has two EF hand domains which are calcium-binding sites. Since calcium has been known to play key roles in ... including interstitial chromosome translocation, loss of heterozygosity, and aneuoploidy. Moreover, unprotected chromosome ends ...
  https://digital.library.unt.edu/search/?q5=%22Cell%20Division%22&t5=dc_subject&searchType=advanced
*  FTY720 Increases CD74 Expression and Sensitizes Cancer Cells to Anti-CD74 Antibody-Mediated Cell Death - Patent application
... diphtheria toxin, Pseudomonas exotoxin, and Pseudomonas endotoxin. 18. The method of claim 13, wherein the therapeutic agent is ... 0069] A single chain Fv molecule (scFv) comprises a VL domain and a VH domain. The VL and VH domains associate to form a target ... 1999; 6:222). Binding of cAMP to the R subunits leads to the release of active catalytic subunits for a broad spectrum of ... We also show that FTY720-induced cell death is mediated by lysosomal membrane permeabilization with subsequent translocation of ...
  http://www.patentsencyclopedia.com/app/20130022541
*  SYNERGISTIC TREATMENT OF CELLS THAT EXPRESS EPHA2 AND ERBB2 - Patent application
Such proteins may include, for example, a toxin such as abrin, ricin A, pseudomonas exotoxin, cholera toxin, or diphtheria ... antibodies comprising a VL domain disclosed herein combined with a VH domain disclosed herein, or other known VH domains. [0070 ... Ribozymes are catalytic RNA molecules with ribonuclease activity which are capable of cleaving a single-stranded nucleic acid, ... When linked to another polypeptide, membrane permeable sequences can also direct the translocation of that polypeptide across ...
  http://www.patentsencyclopedia.com/app/20100254996
*  Characterization of Proprotein Convertases and Their Involvement in Virus Propagation | SpringerLink
The catalytic domains are the most conserved domains among the PCs. The architecture of the catalytic active furin domain is ... Evidence for involvement of furin in cleavage and activation of diphtheria toxin. J Biol Chem. 1993;268(35):26461-5.PubMed ... A new member of the proprotein convertase gene family (LPC) is located at a chromosome translocation breakpoint in lymphomas. ... Expression of mouse furin in a Chinese hamster cell resistant to Pseudomonas exotoxin A and viruses complements the genetic ...
  https://link.springer.com/chapter/10.1007%2F978-3-319-75474-1_9
*  METHODS AND KITS FOR DIAGNOSING OR MONITORING AUTOIMMUNE AND CHRONIC INFLAMMATORY DISEASES - Patent application
... diphtheria toxin or pseudomonas exotoxin, to mention just a few examples. In some preferred embodiments, deglycosylated ricin A ... In one embodiment, a fusion protein can be provided which adds a domain that allows one or both of the proteins to be bound to ... translocation of the RNA to the site of protein translation, translation of protein from the RNA, splicing of the RNA to yield ... one or more mRNA species, and catalytic activity that may be engaged in or facilitated by the RNA. The overall effect of such ...
  http://www.patentsencyclopedia.com/app/20110256121
*  Endometrial Phase or Endometrial Cancer Biomarkers - Patent application
Examples of such agents include abrin, ricin A, Pseudomonas exotoxin, or diphtheria toxin; proteins such as tumor necrosis ... four-disulfide core domain 2. The amino acid sequence for native human WAP four-disulfide core domain 2 includes the amino acid ... Bioluminescence is a type of chemiluminescence found in biological systems where a catalytic protein increases the efficiency ... Method and apparatus for detecting translocation. 2016-05-26. Nucleic acid amplification method. ...
  http://www.patentsencyclopedia.com/app/20130102004
*  Systems and methods for delivery of a therapeutic agent - The Foundry, LLC
... of the botulinum toxin (BoNT), such as one of the serotype A, B, C, D, E, F or G botulin ... exotoxin A and its active fragments, diphtheria toxin and its active fragments, cholera toxin and its active fragments, tetanus ... F or G botulinum toxins, via permeabilization of targeted cell membranes to enable translocation of the botulinum neurotoxin ... According to the present invention, an active fragment (sometimes referred to as a catalytic portion) of a neurotoxin such as ...
  http://www.freepatentsonline.com/9498283.html
*  INHIBITION OF SECRETION FROM NON-NEURONAL CELLS - Patent application
... toxin translocation domain. 4. The method according to claim 3, wherein said clostridial toxin translocation domain is provided ... These include, for example, diphtheria toxin [London, E. (1992) Biochem. Biophys. Acta., 1112, pp. 25-51], Pseudomonas exotoxin ... 6 for an illustration of the in vitro catalytic activity of LHN/B. Example 5 Production of a Conjugate of a Lectin from ... toxin translocation domain. 15. The method according to claim 14, wherein said clostridial toxin translocation domain is ...
  http://www.patentsencyclopedia.com/app/20120101027
*  Transferase other than ribonuclease (2.) patent application class
Catalytic domains of beta(1,4)-galactosyl transferase i having altered donor and acceptor specificities, domains that promote ... METHODS OF PURIFICATION OF NATIVE OR MUTANT FORMS OF DIPHTHERIA TOXIN - The present invention relates to the use of ... MODIFIED FORMS OF PSEUDOMONAS EXOTOXIN A - exotoxin A or "PE" is a 66 kD, highly potent, cytotoxic protein secreted by the ... which can permit transit or translocation of at least one compound across the membrane and cause cell death of the target cell. ...
  http://www.patentsencyclopedia.com/class/000527012
*  21132, a Human G-protein coupled receptor family member and uses therefor - Patent application
Such proteins may include, for example, a toxin such as abrin, ricin A, pseudomonas exotoxin, or diphtheria toxin; a protein ... extracellular domain, a C-terminal intracellular domain, intracellular loop domains, extracellular loop domains, or ... Alternatively, 21132 mRNA can be used to select a catalytic RNA having a specific ribonuclease activity from a pool of RNA ... translocation, inversion, or deletion. [0306]For example, detecting the genetic lesion can include: (i) providing a probe/ ...
  http://www.patentsencyclopedia.com/app/20090130681