Loading...
*  Single-Cell Transfection by Electroporation Using an Electrolyte/Plasmid-Filled Capillary
Thus, transfection success is closely related to the small molecule mass transport dynamics as indicated by the loss of ... The EGFP expression is weaker than bulk lipid-mediated transfection, as indicated by the EGFP fluorescence intensities. However ... the success with the single-cell approach is considerably greater than lipid-mediated transfection. ... Single-cell transfection of adherent cells has been accomplished using single-cell electroporation (SCEP) with a pulled ...
  http://publications.lib.chalmers.se/publication/107247-single-cell-transfection-by-electroporation-using-an-electrolyteplasmid-filled-capillary
*  A Spanish breakthrough allows the electroporation of cell cultures for less t... ( Researchers from the Polytechnic Uni...)
This process is called transfection and in 2012 it generated a global turnover of 650 million euros, according to data from the ... Garca-Snchez explains to SINC that during the process of developing the new system they experimented with a transfection ...
  http://bio-medicine.org/biology-news-1/A-Spanish-breakthrough-allows-the-electroporation-of-cell-cultures-for-less-than-1-Euro-28764-1/
*  Generator Only - Ecm 830 Square Wave Electroporation System, Btx - Model 45-0052 - Each
The unit can be used for applications such as mammalian cell transfections/gene therapy and cell protein/drug ...
  http://www.egeneralmedical.com/vwr-47745-928.html
*  Patent US20070043345 - Tissue ablation with irreversible electroporation - Google Patents
Methods for promoting cell transfection in vivo. US20020077676 *. 14 Dec 2001. 20 Jun 2002. Schroeppel Edward A.. Implantable ...
  http://www.google.ca/patents/US20070043345
*  Polyethyleneimine functionalized iron oxide nanoparticles as agents for DNA delivery and transfection - Journal of Materials...
... efficient DNA delivery and transfection agent has been developed by chemically attaching short chain polyethyleneimine (PEI) ... as agents for DNA delivery and transfection S. C. McBain, H. H. P. Yiu, A. El Haj and J. Dobson, J. Mater. Chem., 2007, 17, ... A novel, efficient DNA delivery and transfection agent has been developed by chemically attaching short chain polyethyleneimine ...
  http://pubs.rsc.org/en/Content/ArticleLanding/2007/JM/b617402g
*  Patente US7718409 - Controlled electroporation and mass transfer across cell membranes - Google Patentes
"Highly Efficient Transfection of Mammalian Cells by Electric Field Pulses: Application to Large volumes of Cell Culture by ... Methods for promoting cell transfection in vivo. US20020077676. 14 Dic 2001. 20 Jun 2002. Schroeppel Edward A.. Implantable ... A high electric field, for example, may thus produce an increase in transfection efficiency in one portion of a cell population ... Rols, M.P., et al., Highly Efficient Transfection of Mammalian Cells by Electric Field Pulses: Application to Large Volumes of ...
  http://www.google.es/patents/US7718409?dq=flatulence
*  Different Incubation Times of Cells After Gene Electrotransfer in Fetal Bovine Serum Affect Cell Viability, but Not...
On the other hand, transfection efficiency of gene electrotransfer was not affected by long incubation of cell in FBS, ... Gene electrotransfer Fetal bovine serum Melanoma cells Endothelial cells Cell survival Transfection efficiency ... Different applications of gene electrotransfer require high viability of cells and high transfection efficiency of gene ... Optimization of a gene electrotransfer method for mesenchymal stem cell transfection. Gene Ther 15:537-544CrossRefPubMedGoogle ...
  https://link.springer.com/article/10.1007/s00232-014-9649-9
*  Gene Pulser Xcell™ Electroporation Systems | Life Science Research | Bio-Rad
Preset transfection protocols for common mammalian and bacterial cells; easy programming. ... The Transfection Protocol Online Library contains protocols obtained from the literature, developed by Bio-Rad scientists, or ... Home>Life Science Research>Products>Transfection>Electroporation,Gene Pulser Xcell™ Electroporation Systems ... These transfection instruments are cited extensively in the literature. Our chemically mediated methods include lipid-based ...
  http://www.bio-rad.com/en-us/product/gene-pulser-xcell-electroporation-systems?pcp_loc=lnav
*  Patent US7521224 - Microelectronic cell electroporation array - Google Patents
These gene insertion methods include, viral transfection, chemical weakening of the cell membrane and microinjection of the ...
  http://www.google.com/patents/US7521224?dq=No.+6,411,949&ei=AUR7T-LGJqSr0AHy2aSiBg
*  Transfection
GeneXPlus Transfection Reagent. GeneXPlus Transfection Reagent is designed to efficiently transfect a broad spectrum of cell ... TransfeX™ Transfection Reagent. TransfeX™ Transfection Reagent has been optimized for use on a wide range of cell types, ... Our transfection reagents are cationic lipid-based; due to their positive charge, these transfection reagents interact with the ... ATCC® Transfection reagents may be used for the transfer of nucleic acids for a variety of applications, including protein ...
  https://www.atcc.org/en/Products/Transfection.aspx?utm_id=t170206185l1
*  Nucleic Acid Transfection | SpringerLink
Strategies for the Preparation of Synthetic Transfection Vec ... DNA Synthetic Transfection Vectors Targeted siRNA Transfection ... Strategies for the Preparation of Synthetic Transfection Vectors, by Asier Unciti-Broceta, Matthew N. Bacon, and Mark Bradley. ... Hyperbranched Polyamines for Transfection, by Wiebke Fischer, Marcelo Calderon, and Rainer Haag. * ... Chemically Programmed Polymers for Targeted DNA and siRNA Transfection, by Eveline Edith Salcher and Ernst Wagner. * ...
  https://link.springer.com/book/10.1007/978-3-642-16430-9
*  Transfection problems
To date, all my transfections were unsuccessfull. Does , anybody have any ideas why the transfections won't work? , , Thanks , ... Transfection problems. John Ladasky jladasky at pmgm.Stanford.EDU Thu Jan 29 18:50:09 EST 1998 *Previous message: Transfection ... construct using the calsium phosphate method of transfection. The DNA I , used for this transfection was isolated with ... Rentia I have used Promega's Wizard minipreps and maxipreps to prepare plasmid DNA that was suitable for transfection. The cell ...
  http://www.bio.net/bionet/mm/methods/1998-January/064258.html
*  Supercoiled DNA/transfection
... Vladimir Svetlov svetlov at oncology.wisc.edu Tue Aug 26 14:36:02 EST 1997 *Previous message: ... Good luck with transfection with DNA ligated in presence of EtBr. Yours, Pointless peasant Baldrick. *Previous message: ...
  http://www.bio.net/bionet/mm/methods/1997-August/060431.html
*  Transfection with Lambda
... Bruce Elder elder at CGL.UCSF.EDU Tue Jun 14 14:57:14 EST 1994 *Previous message: Undergrad. computer ...
  http://www.bio.net/bionet/mm/methods/1994-June/015264.html
*  Reporter Assays and Transfection
... luciferase-expressing cell lines and low-toxicity transfection reagents to ensure successful delivery of vectors to the cell ... Transfection Reagents. Proven, well-cited transfection solutions for a range of cell types, including primary cells and iPSCs. ... Reporter Assays and Transfection. Browse the product groups below to find reporter assays, a comprehensive range of reporter ... vectors, luciferase-expressing cell lines and low-toxicity transfection reagents to ensure successful delivery of vectors to ...
  https://www.promega.com/products/reporter-assays-and-transfection/
*  Transfection of HepG2 cells
... kathy gately kathy.gately at ucg.ie Mon Jul 6 14:53:41 EST 1998 *Previous message: Smeared ... Hi, Has anybody transfected HepG2 cells with SV40-CAT or CMV enhanced tk-CAT? I have done several transfections and I've used ...
  http://www.bio.net/bionet/mm/methods/1998-July/068370.html
*  transfection problem: DNA purity
When I used stock pcDNA-lacZ for , transfection, everything worked fine and b-gal was obviously detected by , western blot ... transfection problem: DNA purity. Bruno Cenni bruno.removethis.cenni at removethis.insel.ch Mon Nov 19 02:11:57 EST 2001 * ... I've a problem during transfection. I tried to transfect pcDNA-lacZ to , mammalian cells using lipofectamine plus. ...
  http://www.bio.net/bionet/mm/methods/2001-November/091236.html
*  TransMessenger Transfection Reagent Handbook ( ...)
Transfection,Reagent,Handbook,biological,advanced biology technology,biology laboratory technology,biology device technology, ... For transfection of eukaryotic cells with RNA and siRNA Contents Kit Contents Storage and Stability Quality Control a href #Te, ... Optimizing siRNA Transfection. Guidelines for Transfection of siRNA Duplexes Using TransMessenger Transfection Reagent. ... TransMessenger Transfection Reagent. 2. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection ...
  http://bio-medicine.org/biology-technology/TransMessenger-Transfection-Reagent-Handbook-723-1/
*  Transfections...?
... Eric Tsai ericbt at umich.edu Wed Jan 14 11:56:58 EST 1998 *Previous message: Transfections...? ... I am using, primarily, the neomycin gene (G418). I would like to follow this with a secondary transfection using the psv2gpt ... If I am doing a second transfection, would my selection medium contain both G418 and the resistance reagents for psv2gpt and ... Hi, everyone! I have a question about subsequent transfections. ... As far as selection medium following the first transfection, I ...
  http://www.bio.net/bionet/mm/cellbiol/1998-January/008045.html
*  Transfection - BioForum
Javascript Disabled Detected You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.. ...
  http://www.protocol-online.org/forums/videos/category-39-transfection/?sort_by=date&sort_order=desc&listview=1
*  INTERCHIM: Transfection
Transfection. For consistent success in your Transfection and 'silencing' RNA applications (to inhibit and suppress target ... LIFE SCIENCES > Molecular Biology - Genetics > Transfection'>LIFE SCIENCES > Molecular Biology - Genetics > Transfection. ... our chemical-transfection agent UptiFectin™OFF. This is a patented synthetic derivative of a natural compound designed ...
  http://www.interchim.com/pp/465/transfection.html
*  What is Transfection? (with pictures)
Transfection is the process of introducing certain nucleic acids into a eukaryotic cell by means other than a virus. While the ... Two main types of transfection exist: transient transfection and stable transfection. In transient transfection, the DNA is ... In stable transfections, the new DNA becomes part of the cell's original DNA by either adding on to it or replacing a piece of ... Transfection is the process of introducing certain nucleic acids into a eukaryotic cell by means other than through a virus. ...
  https://www.wisegeek.com/what-is-transfection.htm
*  Transfection-Tissue culture | Physics Forums
Or does it only matter at transfection? How do I know that my cells are in the mid log phase? How do i... ... confluent and preferably not in colonies for optimal transfection. I usually split my cells 24 hours before transfection. ... Should I always split my cells at their mid log phase? Or does it only matter at transfection? How do I know that my cells are ... Its all rough estimates, 80-100% means usually a full dish of cells, depending on the transfection methods and the cell lines ...
  https://www.physicsforums.com/threads/transfection-tissue-culture.67889/
*  TransPass™ R2 Transfection Reagent | NEB
R2 Transfection Reagent is a two component non-liposomal reagent combination developed specifically for efficient transfection ... siRNA Transfection Guidelines: *The transfection of siRNA must be optimal in order to obtain maximum silencing of a target gene ... The transfection efficiency of plasmic DNA varies from cell line to cell line. For efficient DNA transfection we recommend the ... In order to easily estimate the efficiency of transfection of particular cell lines use Fluorescein-siRNA Transfection Control ...
  https://www.neb.com/products/m2552-transpass-r2-transfection-reagent