N-Myc downstream-regulated gene 1 (NDRG1) plays vital roles in tumor metastasis suppression and is frequently silenced in ... Transcriptional silencing of N-Myc downstream-regulated gene 1 (NDRG1) in metastatic colon cancer cell line SW620 Clin Exp ... N-Myc downstream-regulated gene 1 (NDRG1) plays vital roles in tumor metastasis suppression and is frequently silenced in ... in agreement with its gene expression level. The low Pol II binding at the NDRG1 promoter in SW620 was associated with gene- ...

Myc). Using Saccharomyces cerevisiae, we have shown that the Max bHLH/LZ domain enables Myc to activate transcription through ... whilst helix 1 is essential for sequence-specific DNA binding of Myc-Max complexes. Furthermore, the ability of Myc HLH/LZ ... In addition, we have used yeast to investigate the role of the Myc helix-loop-helix (HLH) and leucine zipper (LZ) motifs in ... The results show that, while both motifs are essential for Myc to activate transcription, helix 2 of the HLH together with the ...

In particular, the Myc protein amplifies transcription by binding to promoters and enhancers of active genes where it directly ... Commonly used gene expression experiments interrogate the expression of one gene (qPCR) or many genes (microarray, RNA-Seq). ... In cancer, Myc-driven transcriptional amplification is posited to help tumor cells overcome rate-limiting constraints in growth ... Transcriptional amplification is caused by changes in the amount or activity of transcription-regulating proteins. Gene ...

MYC) is one of the most frequently overexpressed genes in breast cancer that drives cancer stem cell-like traits, resulting in ... and a transcriptional regulatory circuitry encompassing MYC, ZNF148, and ID1/3 in driving cancer stem cell traits in aggressive ... as a direct target of MYC. ZNF148 suppressed cell proliferation and migration and was transcriptionally repressed by MYC in ... as previously uncharacterized targets of transcriptional repression by ZNF148. Silencing of ZNF148 increased the stemness and ...

Hippo regulates growth and progenitor genes like Sox2, Snai2, Ccdn1, Cdc20, and l-Myc in cardiomyocytes. In livers ... Lats kinases complex with Mob to phosphorylate Yap and Taz, two related transcriptional coactivators. ... Dual-Luciferase Reporter Gene Assay. The pmirGLO luciferase reporter vectors containing wild and mutant 3. -UTR sequence of ... Mammals have two Lats genes, Lats1 and Lats2, which are homologous to Drosophila Warts. ...

Therefore, transcriptional repression by Myc could play a crucial role in regulating the expression of this gene. Our data led ... One of the target genes of TCF-4 is Myc, which encodes a transcription factor, c-Myc (herein called Myc). This factor can ... Gartel AL, Shchors K. Mechanisms of c-myc-mediated transcriptional repression of growth arrest genes. Exp Cell Res ... The assay revealed that HMGCS2 is a direct target of c-Myc, which represses HMGCS2 transcriptional activity. c-Myc ...

Onvansertib suppresses MYC expression and transcriptional Activity. *Substantially downregulated MYC target genes in ... Significant enhancement in both cell lines G2M checkpoint, mitotic spindle, MYC targets, P53 pathway, and apoptosis gene sets ... Notable reduction of c-MYC protein abundance in medulloblastoma cells. *Affected ~50% differential expressed genes regulated by ... PLK1 is a top negatively selected gene.. An investigation of PLK1 expression in DepMap found PLK1 is a critical mediator in 55 ...

Transcriptional activation targets downstream genes such as NFκB, MYC, and BCL2 (2). ... Another potential target in ACC-I is the apoptotic protein BCL2, and again upregulation of the gene with MYC and NICD1 ... Mechanistically, Notch signaling activation is thought to drive MYC transcriptional upregulation and overexpression (4). Of ... mutant cases as part of an ACC-I subclass that collectively demonstrate upregulation of other hyperactivating genes such as MYC ...

GADD45B, MAFF, and MYC were identified and validated as the hub genes. Finally, anisomycin and MG-262 were predicted to target ... GADD45B, MAFF, and MYC were identified and validated as the hub genes. Finally, anisomycin and MG-262 were predicted to target ... database to identify the candidate target genes and potential compounds. Four groups of co-expressing genes were identified as ... database to identify the candidate target genes and potential compounds. Four groups of co-expressing genes were identified as ...

MYC encodes a transcriptional regulator that modulates expression of genes controlling cell growth, proliferation, metabolism, ... The MYC gene family plays essential roles in normal development and in multiple cellular functions. Moreover aberrant MYC gene ... Cellular MYCro Economics: Balancing MYC Function with MYC Expression. David Levens. MYC and Transcription Elongation. Peter B. ... MYC and the Control of Apoptosis. Steven B. McMahon. c-MYC-Induced Genomic Instability. Alexandra Kuzyk and Sabine Mai. MYC and ...

MeCP2 and methylation of tumor-related genes in human gastric cancer ... we examined the methylation and transcriptional level of tumor-related genes including p16INK4A, hMSH2 and c-myc, and detected ... Methyl-specific PCR (MSP) for p16INK4A and c-myc genes. To amplify the promoters of p16INK4A and c-myc genes, we carried out ... The transcriptional levels of p16INK4A and MeCP2 genes did not display any difference between gastric cancerous and matched non ...

As shown in Figure 3B, we observed a significant regulation of most of these genes (e.g., c-Myc [Myc]; claudin 1 [Cldn1], ref. ... The RIP140 gene is a transcriptional target of E2F1. PLoS One. 2012;7(5):e35839.. View this article via: PubMed CrossRef Google ... RIP140 expression was inversely correlated with β-catenin target genes such as c-Myc (MYC) or gastrin (GAST) in the whole ... 26, 27, and Figure 6C). In order to demonstrate transcriptional regulation of the APC gene by RIP140, we transiently ...

... and PRMT5-containing Brg1 complex is involved in transcriptional repression of the Myc target gene cad. Pal, S., Yun, R., Datta ... Gene context of aminomethanone. *These observations prompted us to examine the expression profile of the c-Myc target genes, ... wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary ... DNA sequence of the carA gene and the control region of carAB: tandem promoters, respectively controlled by arginine and the ...

Run-on analysis showed transcriptional attenuation in the normal rat myc gene, similar to the situation in mice and humans. The ... Transcriptional deregulation of myc in IgH/myc 6;7 translocation carrying rat immunocytomas Author ... Transcriptional deregulation of myc in IgH/myc 6;7 translocation carrying rat immunocytomas ... of the intact gene, proximal to the myc promoters. In this paper we describe the effect of the translocation on myc ...

... in single-cell sequencing enable recovery of the complete adaptive immune receptor sequences in addition to transcriptional ... We could relate clonal expansion, germline gene usage, and clonal convergence to cell phenotypes spanning activation, memory, ... S2A). This was characterized by increased expression of genes associated with fatty acid metabolism, hypoxia and Myc targets, ... Mitochondrial and ribosomal genes were removed when visualizing DE genes. Gene expression heatmaps, feature plots, and violin ...

The gene encoding products of AREs and GREs, such as C-MYC, Epidermal Growth Factor (EGF), Prostaglandin-Endoperoxide Synthase ... thereby reducing the stability and transcriptional activity of ARE- or GRE-mRNAs (81, 82). Indeed, compared to normal tissues, ... Gene-based genome-wide association analysis in european and asian populations identified novel genes for rheumatoid arthritis. ... small RNA-mediated gene silencing technology has become a weapon in gene function research and has been widely used. Similar to ...

Lastly, IL-21 also promotes activation of the STAT3 promoter and other transcriptional factors. These findings may be relevant ... By activating these transcriptional factors, IL-21 has broader regulatory roles that will likely impact on expression of genes ... In addition, IL-21 regulates other transcriptional factors including Smad3/4, AP-2, Pax-5, C-myc, MEF-1 and CRE via partial ... Assays for transcriptional activity based on the luciferase reporter gene. In: Tymms MJ, editor. Transcription Factor Protocols ...

55 but MYC has a complex transcriptional and post-transcriptional regulation which acts to strictly limit the levels of MYC in ... the coding region of the MYC gene is transferred intact. The breakpoint in the immunoglobulin gene to which MYC is transferred ... A defining feature of BL is the reciprocal translocation between the MYC gene and one of the three immunoglobulin genes: the ... A key aspect of MYC function in BL cells is the phenomenon of MYC-induced apoptosis. While MYC potently drives S phase ...

... ends of genes where it promotes transcriptional competence. How DNA methylation and H2A.Z influence transcription remains ... Finally, overexpression of Myc, a known suppressor of p21 synthesis, significantly increases H2A.Z localization at the Myc- ... The variant histone H2A.Z regulates gene transcription, and deletion of the gene encoding H2A.Z strongly increases the ... Microarray analysis revealed that HTZ1-activated genes cluster near telomeres. The reduced expression of most of these genes in ...

NICD/CSL-dependent transcriptional activation of target genes, such as BMI1, CCND1, CD44, HES1, HEY1, MYC, NOTCH3, REST and ... Robbins J, Blondel BJ, Gallahan D and Callahan R: Mouse mammary tumor gene int-3: A member of the Notch gene family transforms ... Maier MM and Gessler M: Comparative analysis of the human and mouse Hey1 promoter: Hey genes are new Notch target genes. ... Panel-based genomic tests detecting mutations and other alterations in 400~500 cancer-related genes, FISH detecting gene Amp or ...

... cells confirmed inhibition of MYC-related transcriptional activity and revealed an enrichment of HOXA5 target gene expression. ... Using a transwell invasion assay, we discovered 33 genes essential for invasion. Of the 11 genes we selected for secondary ... Transcriptional changes in astrocytes are accompanied by alterations in phagocytic capacity and effects on neuronal calcium ... Here, we demonstrate high levels of GABRA5, which encodes the α5-subunit of the GABAA receptor complex, in aggressive MYC- ...

Reducing MYCs transcriptional footprint unveils a good prognostic gene signature in melanoma  Zacarias Fluck, Mariano F; ...

BHLHE40 plays a pathological role in pre-eclampsia through upregulating SNX16 by transcriptional inhibition of miR-196a-5p. Mi ... SNX16 activates c-Myc signaling by inhibiting ubiquitin-mediated proteasomal degradation of eEF1A2 in colorectal cancer ... Gene neighbors Overlapping genes and two nearest non-overlapping genes on either side ... Observational study of gene-disease association. (HuGE Navigator) Title: Evaluation of nine candidate genes in patients with ...

1997) HBP1: a HMG box transcriptional repressor that is targeted by the retinoblastoma family Genes & Development 11:383-396. ... including N-Myc and DNA methyltransferase 1 (DNMT1), but also transcriptionally activates genes, such as p16, p21 and histone ... Hbp1 executes dual transcriptional functions by directly binding high-affinity elements on negatively regulated target genes, ... All genes in this study except RanBP9 were cloned from human cDNA using standard PCR protocols. The N-terminus of RanBP9 (bps 1 ...

Molecular cloning and transcriptional mapping of the mouse cellular retinoic acid-binding protein gene., Nguyen-Huu MC, ... Embryonic lethality in mice homozygous for a targeted disruption of the N-myc gene., Alt FW, Robertson EJ, Goff SP, Jeannotte L ... YY1 acts as a transcriptional activator of Hoxa5 gene expression in mouse organogenesis., Jeannotte L, Prudhomme C, Bérubé- ... Identification of Notch target genes in uncommitted T-cell progenitors: No direct induction of a T-cell specific gene program. ...

Upon PRMT6 overexpression, transcription of Hox genes and Myc-dependent genes, both well-known targets of H3 K4 trimethylation ... Transcriptional activation of a gene involves an orchestrated recruitment of components of the basal transcription machinery ... Transcriptional regulation of the human ferritin gene by coordinated regulation of Nrf2 and protein arginine methyltransferases ... Specific sites of histone tail methylation are associated with transcriptional activity at gene loci. These methyl marks are ...

L-myc, a new myc-related gene amplified and expressed in human small cell lung cancer ... Human c-myc onc gene is located on the region of chromosome 8 that is translocated in Burkitt lymphoma cells ... Myc regulates a transcriptional program that stimulates mitochondrial glutaminolysis and leads to glutamine addiction (English) ... Myc regulates a transcriptional program that stimulates mitochondrial glutaminolysis and leads to glutamine addiction. ...

... transcriptional co-activator translocates into the nucleus of cells to transcriptionally activate Hippo pathway target genes ( ... S5C). Some of these TFs are predicted to act in several cell subsets [e.g. MYC and TP53 (TRP53)], but most display selectivity ... the gene groups (and the number of genes in each); right: representative enriched genes and the most statistically significant ... the gene groups (and the number of genes in each); right: representative enriched genes and the most statistically significant ...

Most genes involved in the isoflavonoids biosynthesis had the lowest expression in the leaves, and the highest in the stems. ... For triterpene saponin biosynthesis, we found the genes in MVA and non-MVA pathways were differentially expressed among three ... Therefore, a global transcriptome analysis on A. mongolicus tissues was performed to identify the genes essential for the ... encodes a protein similar to transcriptional activators and contains the myc-homology region. Proceedings of the National ...

... PubMed, SCI ... Then, the gene expression level of each sample was calculated using RSEM [35]. The expressed genes were further analyzed ... Oncogenes; β-catenin, K-Ras (p21ras), c-myc, TERT, receptor tyrosine kinase c-MET, PIK3CA ... were used to get the target gene of differential expressed miRNA and extract intersection or union of target gene as final ...