*  Frontiers | Gut Microbiota and Developmental Programming of the Brain: From Evidence in Behavioral Endophenotypes to Novel...
... or bacterial metabolic activities characterize also the gut microbiota of obese individuals (Manco et al., 2010). Gut dysbiosis ... This phenomenon, which extends to peri- and post-natal periods, is known as "developmental programming of health and disease" ( ... Microbial colonization of mammals is an evolution-driven process that modulates many host physiological functions, many of ... the set of behavioral/physiological symptoms which result into more stable phenotypes of complex traits characterized by low ...
  https://www.frontiersin.org/articles/10.3389/fcimb.2012.00109/full
*  ARTICLES | Physiological Genomics
To further characterize the complex phenomena associated with this common bacterial product, we studied differential gene ... available at the Physiological Genomics web site).1 Total RNA from the PBMC of four subjects was extracted as above, treated ... Physiological Genomics Published 13 April 2006 Vol. 25 no. 2, 203-215 DOI: 10.1152/physiolgenomics.00192.2005 ... Within 1 h, total leukocyte counts reached their nadir due to margination of cells to activated vascular endothelium (31, 39, ...
  http://physiolgenomics.physiology.org/content/25/2/203
*  Protocols and Video Articles Authored by Jongyoon Han
The total passage time can be divided into two components: the entry time required for a cell to deform and enter a ... This phenomenon displaces the leukocytes to the vessel wall and is aptly termed as margination. Here, we demonstrate using ... We characterize this method by comparing the selectivity with that achieved in devices with different dimensions. The results ... Feb, 2017 , Pubmed ID: 28024083 Mammalian tissues rely on a variety of nutrients to support their physiological functions. It ...
  https://www.jove.com/author/Jongyoon_Han
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Developmental Biology, Issue 79, Eukaryota, Biological Phenomena, Cell Physiological Phenomena, C. elegans, cell culture, ... total cellular RNA. In many cases changes in total cellular RNA occur either too slowly or too quickly to represent the ... Many intracellular bacterial pathogens use freshwater protozoans as a natural reservoir for proliferation in the environment. ... We have designed a high-density microarray, in Affymetrix format, aiming to optimally characterize individual HERV loci ...
  https://www.jove.com/visualize/abstract/19204800/microarray-analyses-gene-expression-during-tetrahymena-thermophila
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Bioengineering, Issue 80, Cardiovascular Diseases, Circulatory and Respiratory Physiological Phenomena, Fluid Mechanics and ... In patients with end-stage heart failure (HF), a total artificial heart (TAH) may be implanted as a bridge to cardiac ... The use of transgenic mice and defined bacterial mutants makes this model particularly suitable for identifying both host and ... Aortic diameter is measured for each pressure step to characterize the pressure-diameter relationship of the ascending aorta. ...
  https://www.jove.com/visualize/abstract/22723973/aortic-calcification-femoral-bone-density-are-independently
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
To characterize the metabolically active bacterial species in the gut of an insect, we used stable isotope probing (SIP) in ... Biological Phenomena, Chemical Phenomena, Metabolic Phenomena, Microbiological Phenomena, Earth Resources and Remote Sensing, ... A physiological stress response by herbivores to the risk of predation can change the C:N elemental composition of herbivore ... Stellate cells are derived from bone marrow precursors and store up to 80% of the total body vitamin A1, 2. Upon activation, ...
  https://www.jove.com/visualize/abstract/22479477/variation-basal-erod-activities-ten-passerine-bird-species
*  Protocols and Video Articles Authored by Fabio Cafini (Translated to Korean)
Decrease in Bacterial Load Versus Resistance Selection of Pneumococcal Subpopulations by Beta-lactam Physiological ... At time zero, the recombined population was 0.00095% of the total population. In controls, the BL⁻ and BL⁺ total populations ... Jul, 2008 , Pubmed ID: 18648650 Although protein binding is a reversible phenomenon, it is assumed that antibacterial activity ... Dec, 2008 , Pubmed ID: 18799472 To phenotypically and genotypically characterize 11 strains (isolated in four different centres ...
  https://www.jove.com/author/Fabio_Cafini?language=Korean
*  Protocols and Video Articles Authored by Michael D. Menger (Translated to Hindi)
A total of seven IMAs were prepared in their full intrathoracic length. A change in the preparation technique (lateral ... To characterize the type of bone repair, we performed additional histomorphometric analyses at days 3-15. IVM was conducted in ... Intraabdominal Bacterial Infections Significantly Alter Regeneration and Function of the Liver in a Rat Model of Major ... Moreover, a leukocyte-endothelial cell interaction was found in a physiological range at the implantation site of all three ...
  https://www.jove.com/author/Michael++D._Menger?language=Hindi
*  Genetics - Medical Microbiology - NCBI Bookshelf
These latter characteristics are often selected as the inherited traits to be analyzed in studies of bacterial genetics. ... Bacterial viruses (bacteriophages or phages) have DNA or RNA as genetic material. The two essential functions of genetic ... This phenomenon is called diauxic growth. The failure to induce the lac or ara operon in the presence of glucose is an example ... As an example, consider using a complementation test to characterize two independently derived Lac- mutants of E. coli. The ...
  https://www.ncbi.nlm.nih.gov/books/NBK7908/?report=reader
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... identify bacterial species that contain class 1 integrons; and characterize these integrons and their associated gene cassettes ... Musculoskeletal and Neural Physiological Phenomena, HIV, HIV-1, AIDS, Frailty, Depression, Weight Loss, Weakness, Slowness, ... In total, these integrons have acquired and disseminated over 130 different antibiotic resistance genes. With continued ... observation of the BRET phenomenon requires that the donor and acceptor be in close proximity. To test for an interaction ...
  https://www.jove.com/visualize/abstract/26087013/clonal-evolution-blast-crisis-correlate-with-enhanced-proteolytic
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Biological Phenomena, Chemical Phenomena, Metabolic Phenomena, Microbiological Phenomena, Earth Resources and Remote Sensing, ... A physiological stress response by herbivores to the risk of predation can change the C:N elemental composition of herbivore ... A 1,000 m2 facility was constructed which consists of 24 individual 33.6 m2 field plots, each equipped for measuring total ... Parameters addressed with these protocols yield the minimum on information essential to characterize and understand the ...
  https://www.jove.com/visualize/abstract/24223215/ecosystem-service-tradeoffs-associated-with-switching-from-annual-to
*  Protocols and Video Articles Authored by José Prieto
However, there are very few bacterial species in which this phenomenon has been documented. The aim of this work was to assess ... Decrease in Bacterial Load Versus Resistance Selection of Pneumococcal Subpopulations by Beta-lactam Physiological ... At time zero, the recombined population was 0.00095% of the total population. In controls, the BL⁻ and BL⁺ total populations ... The aim of this study was to isolate and characterize renin inhibitory peptides from the red seaweed P. palmata for use in ...
  https://www.jove.com/author/Jos%C3%A9_Prieto
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
To characterize the metabolically active bacterial species in the gut of an insect, we used stable isotope probing (SIP) in ... estimates of the total bacterial communities when processing poultry feces and litter samples. ... As such, the roles that these mechanisms play in the metabolic memory phenomenon are currently being examined. We have recently ... However, a number of physiological and technical issues can affect the accuracy of the results and appropriate guidelines for ...
  https://www.jove.com/visualize/abstract/25279695/faecal-microbiota-of-cats-with-insulin-treated-diabetes-mellitus
*  The ecology of environmental DNA and implications for conservation genetics | SpringerLink
2007; Paige 2010). Mitochondrial DNA from one species can comprise just 10−8 percent of the total eDNA in freshwater (Turner et ... Afshinnekoo E, Meydan C, Chowdhury S et al (2015a) Geospatial resolution of human and bacterial diversity with city-scale ... Origin: What are the physiological sources of eDNA production?. Despite burgeoning interest and study of eDNA applications ... Microbial metatranscriptomics provides guidance for using HTS technologies to characterize eRNA (Shi et al. 2009). Quantifying ...
  https://link.springer.com/article/10.1007/s10592-015-0775-4
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Cell Physiological Phenomena, mRNA translation, ribosomes, protein synthesis, genome-wide analysis, translatome, mTOR, eIF4E, ... First, bacterial DNA is extracted along with host tissue DNA from clinical biopsies or slit skin smears (SSS).10 The desired ... Likewise, the total analysis time can be shortened or lengthened depending on the specific intended use, research question, and ... With this protocol, it is possible to follow the kinetics of toxin entry into the cytosol and to characterize inhibitory ...
  https://www.jove.com/visualize/abstract/21647370/snp-based-typing-useful-tool-to-study-bordetella-pertussis
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
To characterize the metabolically active bacterial species in the gut of an insect, we used stable isotope probing (SIP) in ... Biological Phenomena, Chemical Phenomena, Metabolic Phenomena, Microbiological Phenomena, Earth Resources and Remote Sensing, ... A physiological stress response by herbivores to the risk of predation can change the C:N elemental composition of herbivore ... Internal phosphorus (P) loading from lake sediments can account for a substantial portion of the total P load in eutrophic, and ...
  https://www.jove.com/visualize/abstract/22403625/spatial-scales-bacterial-diversity-cold-water-coral-reef
*  Protocols and Video Articles Authored by Russell J. Crawford
... were employed to characterize substrata and bacterial surfaces. Modification of the glass surface resulted in nanometer-scale ... Certain nanoscale surface phenomena are being exploited to promote or prevent the attachment of living cells. However, as yet, ... The results of phylogenetic and genomic analysis, together with physiological and biochemical properties, indicated that the ... of the total fatty acids). The DNA G+C content was 30.1 mol%. A 16S rRNA gene sequence of the isolate was determined and ...
  https://www.jove.com/author/Russell++J._Crawford
*  Composition of the adult digestive tract bacterial microbiome based on seven mouth surfaces, tonsils, throat and stool samples ...
A key known physiological difference between these two regions is the lower redox potential found subgingivally [47]. ... The Human Microbiome Project (HMP) established by the National Institutes of Health aims to characterize the microbiome of a ... This phenomenon was widely observed within this normal population. The phylum TM7 was highly prevalent, detected in at least ... 226 stool samples in total; Additional file 7) and absence from all the upper digestive tract sites (1,879 samples; Additional ...
  https://genomebiology.biomedcentral.com/articles/10.1186/gb-2012-13-6-r42
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Cell Physiological Phenomena, Natural Science Disciplines, Life Sciences (General), circulating cells, extravasation, ... Isolate single bacterial colonies by the streak-plating method. ● Use pour-plating and spread-plating methods to determine the ... Our aim was to develop and characterize a highly reproducible rat syngeneic in vitro model of the BBB using co-cultures of ... This paper details the methodology and the results obtained from a subset of the total females sampled. Of the 230 female ...
  https://www.jove.com/visualize/abstract/23077669/warm-is-better-incubation-temperature-influences-apparent-survival
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Biological Phenomena, Chemical Phenomena, Metabolic Phenomena, Microbiological Phenomena, Earth Resources and Remote Sensing, ... A physiological stress response by herbivores to the risk of predation can change the C:N elemental composition of herbivore ... However, identification of bacterial taxa that transform specific pools of DOC in nature poses a technical challenge. Here we ... Each was loaded with loose straw, carcasses and manure totaling ~95,000 kg. A 40-cm base layer of loose barley straw was placed ...
  https://www.jove.com/visualize/abstract/24039974/different-land-use-intensities-grassland-ecosystems-drive-ecology
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Bioengineering, Issue 80, Cardiovascular Diseases, Circulatory and Respiratory Physiological Phenomena, Fluid Mechanics and ... Our aim was to develop and characterize a highly reproducible rat syngeneic in vitro model of the BBB using co-cultures of ... Here, we describe a technique to quantify total amounts of GLUT4 translocation to the plasma membrane of cells during a chosen ... Bacterial Infections and Mycoses, Mucosal immunity, oleic acid, linoleic acid, linolenic acid, cholera toxin, cholera, fatty ...
  https://www.jove.com/visualize/abstract/22028857/changes-cardiac-substrate-transporters-metabolic-proteins-mirror
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Biological Phenomena, Chemical Phenomena, Metabolic Phenomena, Microbiological Phenomena, Earth Resources and Remote Sensing, ... A physiological stress response by herbivores to the risk of predation can change the C:N elemental composition of herbivore ... Indeed, total biomass, with no marine mammals in the ecosystem, generally remained surprisingly similar, or even decreased for ... Such host-bacterial associations (symbioses) can be detrimental (pathogenic), have no fitness consequence (commensal), or be ...
  https://www.jove.com/visualize/abstract/22970153/marine-mammal-impacts-exploited-ecosystems-would-large-scale-culling
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins. Authors: Savannah E. Sanchez, Daniel A. Cuevas ... identify bacterial species that contain class 1 integrons; and characterize these integrons and their associated gene cassettes ... Therefore, a total of 6 hours is generally needed to complete the collection series described here. ... Research in zebrafish has recently demonstrated that two distinctive phenomena transpire after adult nephrons incur damage: ...
  https://www.jove.com/visualize/abstract/25856230/molecular-barcoding-aquatic-oligochaetes-implications-for
*  Characterizing the Presence and Sensitivity of the P2X7 Receptor in Different Compartments of the Gut - FullText - Journal of...
In this sense, apoptosis constitutes a physiological phenomenon that is expected to affect the highly differentiated ... The lysate was heated to 98°C for 5 min in a sample buffer (Sigma-Aldrich) and 80 µg of total protein was added to each lane. ... Hooper LV, Gordon JI: Commensal host-bacterial relationships in the gut. Science 2001;292:1115-1118. External Resources * ... Nihei OK, Savino W, Alves LA: Procedures to characterize and study P2Z/P2X7 purinoceptor: flow cytometry as a promising ...
  https://www.karger.com/Article/FullText/336628
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... total cellular RNA. In many cases changes in total cellular RNA occur either too slowly or too quickly to represent the ... Among these bacterial pathogens, Listeria monocytogenes is a Gram positive microorganism that has been used as a paradigm for ... Th2 cytokines promote physiological changes in the intestinal microenvironment including rapid turnover of IECs, goblet cell ... A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions ...
  https://www.jove.com/visualize/abstract/22384000/insights-into-scptaps-proteins-liver-flukes-based-on-large-scale