Tracking dendritic cell migration into lymph nodes by using a novel PET probe 18F-tetrafluoroborate for sodium-iodide symporter...
Tracking dendritic cell migration into lymph nodes by using a novel PET probe 18F-tetrafluoroborate for sodium-iodide symporter ... Tracking dendritic cell migration into lymph nodes by using a novel PET probe 18F-tetrafluoroborate for sodium-iodide symporter ... Tracking dendritic cell migration into lymph nodes by using a novel PET probe 18F-tetrafluoroborate for sodium-iodide symporter ... BackgroundRecently, F-tetrafluoroborate TFB was used as a substrate for the human sodium-iodide symporter hNIS reporter gene. ...http://libros.duhnnae.com/2017/jul4/149904577075-Tracking-dendritic-cell-migration-into-lymph-nodes-by-using-a-novel-PET-probe-18F-tetrafluoroborate-for-sodium-iodide-symporter.php
About the mechanism of down regulation of Na + /I - symporter expression in thyroid cancer - Thyroid Disease ManagerThyroid...
Topic: Regulation of Expression of Na + /I - Symporter. Title: PTTG and PBF repress the human sodium iodide symporter.. Authors ... About the mechanism of down regulation of Na + /I - symporter expression in thyroid cancer. May 1, 2007. ... is overexpressed in thyroid tumours and downregulates the expression of the sodium iodide symporter (NIS) in FRTL-5 cells. ...http://www.thyroidmanager.org/2007/05/01/about-the-mechanism-of-down-regulation-of-na-i-symporter-expression-in-thyroid-cancer/
Diarrhea as a cause of mortality in a mouse model of infectious colitis | Genome Biology | Full Text
Additional proteins able to affect intracellular pH include oligopeptide-proton symporters Pept1 and Pept2, whose expression ...https://genomebiology.biomedcentral.com/articles/10.1186/gb-2008-9-8-r122
H1 2017 Ileal Sodium/Bile Acid Cotransporter (Apical Sodium Dependent Bile Acid Transporter or ASBT or Sodium/Taurocholate...
Description: Ileal Sodium/Bile Acid Cotransporter (Apical Sodium Dependent Bile Acid Transporter or ASBT or Sodium/Taurocholate Cotransporting Polypeptidehttps://www.medgadget.com/2017/07/h1-2017-ileal-sodiumbile-acid-cotransporter-apical-sodium-dependent-bile-acid-transporter-or-asbt-or-sodiumtaurocholate-cotransporting-polypeptide-ileal-or-solute-carrier-family-10-member-2-or-slc1.html
Sodium-dependent multivitamin transporter
Human SMVT protein is glycosylated, and that glycosylation is important for its function. The study also shows a role for the putative PKC-phosphorylation site Thr(286) of hSMVT in the PKC-mediated regulation of biotin uptake ...https://pharos.nih.gov/idg/targets/Q9Y289
Lipopolysaccharide inhibits colonic biotin uptake via interference with membrane expression of its transporter: a role for a...
Biotin (vitamin B7), an essential micronutrient for normal cellular functions, is obtained from both dietary sources as well as gut microbiota. Absorption of biotin in both the small and large intestine is via a carrier-mediated process that involves the sodium-dependent multivitamin transporter (SMVT). Although different physiological and molecular aspects of intestinal biotin uptake have been delineated, nothing is known about the effect of LPS on the process. We addressed this issue using in vitro (human colonic epithelial NCM460 cells) and in vivo (mice) models of LPS exposure. Treating NCM460 cells with LPS was found to lead to a significant inhibition in carrier-mediated biotin uptake. Similarly, administration of LPS to mice led to a significant inhibition in biotin uptake by native colonic tissue. Although no changes in total cellular SMVT protein and mRNA levels were observed, LPS caused a decrease in the fraction of SMVT expressed at the cell surface. A role for casein kinase 2 (CK2) ...http://ajpcell.physiology.org/content/312/4/C376.most-cited
Probing the putative active site of YjdL: an unusual proton-coupled oligopeptide transporter from E. coli. - PubMed - NCBI
PLoS One. 2012;7(10):e47780. doi: 10.1371/journal.pone.0047780. Epub 2012 Oct 22. Comparative Study; Research Support, Non-U.S. Gov'thttps://www.ncbi.nlm.nih.gov/pubmed/23110099
Activity-Dependent Cleavage of the K-Cl Cotransporter KCC2 Mediated by Calcium-Activated Protease Calpain | Journal of...
Cell-attached and whole-cell patch-clamp recordings were done at 32°C from visually identified CA1 pyramidal neurons using an EPC 10 amplifier and Pulse software (HEKA). Patch pipettes were fabricated from borosilicate glass (Harvard Apparatus), and their resistance ranged from 4.5 to 6.5 MΩ. The pipette solution consisted of (in mm): 30 N-methyl-d-glucamine-HCl, 95 K-gluconate, 1 EGTA, 5 HEPES, 10 d-glucose, 2 Mg-ATP, 20 sucrose, 0.1 Alexa Fluor 488, 2 NaOH, and 5.4 KOH, pH 7.3. For recordings, slices were positioned in a submerged-type recording chamber and continuously perfused at a rate of 3.5 ml/min with extracellular solution containing (in mm): 124 NaCl, 3.5 KCl, 2 CaCl2, 25 NaHCO3, 1.1 NaH2PO4, 2 MgSO4, and 10 d-glucose, equilibrated with 95% O2 and 5% CO2, pH 7.4. Membrane potential values were corrected for calculated liquid junction potentials (Barry, 1994). Interictal-like activity was induced by omitting Mg2+ from the extracellular solution after recovery and during the recordings ...http://www.jneurosci.org/content/32/33/11356.full
Biotin requirements are lower in human Jurkat lymphoid cells but homeostatic mechanisms are similar to those of HepG2 liver...
The following proteins are candidates for maintaining biotin homeostasis in humans: the biotin transporters sodium-dependent multivitamin transporter (SMVT) and monocarboxylate transporter 1, the biotinyl-protein ligase holocarboxylase synthetase (HChttp://www.biomedsearch.com/nih/Biotin-Requirements-Are-Lower-in/20357078.html
KAKEN - Research Projects | Strucure and function of rat sodium/iodide symporter gene and its expression mechanism in the...
Principal Investigator：ENDO Toyoshi, Project Period (FY)：1997 - 1998, Research Category：Grant-in-Aid for Scientific Research (C), Section：一般, Research Field：内分泌・代謝学https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671037/
By forming signaling complexes through specific interactions with other proteins, G-protein-coupled receptors convert extracellular signals into diverse neuronal responses. In the case of GABABRs, this includes G-proteins that are required for their "classic" signaling, but also interactions with auxiliary proteins that modulate the kinetics of receptor signaling (Schwenk et al., 2010), desensitization (Pontier et al., 2006), subunit dimerization (Couve et al., 2001), and regulate the localization of the receptor or other proteins within cells (White et al., 2000; Boyer et al., 2009). Here we have identified a novel association between the GABABR and the potassium-chloride cotransporter KCC2. This association was discovered in an unbiased screen for proteins present within GABABR complexes at the neuronal membrane, was confirmed by biochemical experiments in hippocampal brain slices and heterologous cells, and was shown to be mediated via the transmembrane region of KCC2. Agonist activation of ...http://www.jneurosci.org/content/37/22/5447
Dietary inclusion of peptides and the effect on the regional expression of the oligo-peptide transporter PepT1 (Slc15a1) in the...
PepT1 (Peptide Transporter 1, SLC15A1) is a proton-coupled peptide transporter responsible for uptake of short peptides and peptide-like drugs from the intestinal lumen into enterocytes. A PepT1-type transporter has recently been sequenced for Atlantic cod and found to be expressed along the whole post-gastric intestine including pyloric caeca. In the present thesis the effect of dietary inclusion of peptides and amino acids on expression of PepT1 mRNA in different regions of juvenile Atlantic cod intestine was investigated. Five groups of cod weighing 10-15 grams were fed for 46 days with a diet containing approximately 42% crude protein, either as fishmeal (FM diet) or 30 % of the fish meal substituted by either whole fish hydrolysate (FH diet), retenate after ultra filtration of fish hydrolysate (UFR diet), retenate after nano filtration of fish hydrolysate (NFR diet) or a mix of free amino acids (FAA diet). After 14 days (T1) and at the end of the experiment (46 days; T3), three fish were ...http://bora.uib.no/handle/1956/3239
Effects of High- Versus Moderate-Intensity Training on Neuroplasticity and Functional Recovery After Focal Ischemia | Stroke
Methods-Rats were randomized as follows: control (n=15), SHAM (n=9), middle cerebral artery occlusion (n=13), middle cerebral artery occlusion at day 1 (n=7), MOD (n=13), and HIT (n=13). Incremental tests were performed at day 1 (D1) and 14 (D14) to identify the running speed associated with the lactate threshold (SLT) and the maximal speed (Smax). Functional tests were performed at D1, D7, and D14. Microglia form, cytokines, p75NTR (pan-neurotrophin receptor p75), potassium-chloride cotransporter type 2, and sodium-potassium-chloride cotransporter type 1 expression were made at D15. ...http://stroke.ahajournals.org/content/early/2017/09/13/STROKEAHA.117.017962
Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 5 (SLC12A5) Antikörper
Reaktivität: Fledermaus, Rind (Kuh), Hund and more. 115 verschiedene SLC12A5 Antikörper vergleichen. Alle direkt auf antikörper-online bestellbar!http://www.antikoerper-online.de/abstract/Solute+Carrier+Family+12+
April 20, 2017. It's a cruel conundrum: States that could use drugs to save lives are instead using them to kill. In the…. Read more ...http://reverbpress.com/tag/potassium-chloride/
Jason Yau Page - Department of Biochemistry, Oxford University
Members of the proton-coupled oligopeptide transporter (POT) family are responsible for transporting small peptides across the cell membrane. The mammalian PepT1 and PepT2 transporters play a key role in this process for dietary peptide uptake in the small intestine and peptide retention in kidney, respectively, and are capable of recognizing and transporting a diverse library (~8000 combinations) of di- and tri-peptides. Recently, PepT1 has also been shown to transport drugs with peptide-like structures, such as β-lactam antibiotics. This makes PepT1 and PepT2 attractive targets to improve drug uptake and bioavailability within the body.. My work focuses on the functional dynamics of PepTso, a bacterial homologue of PepT1. Given the high substrate promiscuity of these peptide transporters, the binding site must be incredibly accommodating and flexible in order to recognize and transport various substrates. Using solid-state NMR, electron spin resonance, and other biophysical techniques, I will ...http://www.bioch.ox.ac.uk/aspsite/printable.asp?pageid=1357
A SEQUENCE FILE IMPOSSIBILITIES « FAROUCHE OMBRÉ
.. The human intestinal proton-coupled oligopeptide transporter hPEPT1 has been implicated in the neuropeptide N-acetyl-Asp-Glu (NAAG), that were judged by their ability to evoke inward currents with no trivial probabilities. That they are mutually attracting autolisins. With non-trivial probabilities, has been shown to be an N-acetyl - muramoyl-L-alanine amidase that human saliva contains and the…https://faroucheombre.wordpress.com/2006/08/08/a-sequence-file-impossibilities/
The Biology of the Sodium Iodide Symporter and its Potential for Targeted Gene Delivery - Surrey Research Insight Open Access
Adenovirus, gene therapy, measles virus, sodium iodide symporter, radioiodide, radiotherapy, THYROID-CANCER CELLS, PROTEIN-KINASE-A, HUMAN NA+/I-SYMPORTER, DEFECTIVE ADENOVIRAL VECTORS, PAIRED-DOMAIN TRANSCRIPTION, MESSENGER-RIBONUCLEIC-ACID, EXPERIMENTAL I-131 THERAPY, TUMOR-NECROSIS-FACTOR, TRANS-RETINOIC ACID, DENSE CORE VESICLES ...http://epubs.surrey.ac.uk/824006/
KLUEDO | Fachbereich Biologie
In my doctoral thesis, I present new information about the developmental expression pattern of the potassium chloride cotransporter KCC2 in the rat auditory brain stem and the morphometrical effects caused by KCC2 gene silencing in mice. The thesis is divided into 3 Chapters. Chapter 1 is a general introduction which gives a brief outline of the primary ascending auditory pathway in mammals. Also, it provides information about the presence of a large number of inhibitory inputs in the auditory system and how these inputs develop; the involvement of inhibition in the acoustic processing is mentioned. In addition, the role of the KCC2 cotransporter in the shift of GABA/glycine transmission, and thus, in maintaining the normal level of inhibition in the mature brain, is described. The focus of Chapter 2 was to investigate the KCC2 immunofluorescent signal from postnatal day (P) 0 to P60 in four major nuclei of the rats superior olivary complex (SOC), namely the medial nucleus of the trapezoid body ...https://kluedo.ub.uni-kl.de/solrsearch/index/search/searchtype/collection/id/15992/start/0/rows/10/yearfq/2004/sortfield/year/sortorder/desc
NF-kappaB p65 subunit mediates lipopolysaccharide-induced Na(+)/I(-) symporter gene expression by involving functional...
The Gram-negative bacterial endotoxin lipopolysaccharide (LPS) elicits a variety of biological responses. Na(+)/I(-) symporter (NIS)-mediated iodide uptake is the main rate-limiting step in thyroid hormonogenesis. We have recently reported that LPS shttp://www.biomedsearch.com/nih/NF-kappaB-p65-Subunit-Mediates/20667985.html
Abstract 2946: Multimodality Monitoring of Cardiac Cell Therapy by PET and MRI | Circulation
Aim: We assessed the feasibility and usefulness of reporter gene imaging for PET and iron labeling for MRI to monitor transplanted cells.. Methods and results: Human endothelial progenitor cells (EPC) were transduced with both human sodium/iodide symporter (NIS) gene using a retroviral vector and labeled with superparamagnetic iron oxides. Nude rats received an intramyocardial injection of 4 million EPC (control:n =6, labeled:n = 10). MRI and I-124 PET were performed at day1 (n = 10), 3 (4) and 7 (7) after cell transplantation. The cell injection site was visualized as a signal void by MRI and a focal I-124 accumulation by PET at day1. The normalized signal contrast (contrast-to-noise ratio; CNR) (0.8 ± 0.2 vs -0.3 ± 0.1, p,0.001) and percentage of tracer injected dose by PET (4.1 ± 1.0 vs -0.2 ± 0.4, p,0.001) were significantly higher in labeled than in non-labeled EPC. CNR remained unchanged at day3 (3.9+/-0.6) and day7 (4.4 ± 1.3). In contrast, I-124 uptake disappeared at 3d (0.42 ± ...http://circ.ahajournals.org/content/116/Suppl_16/II_657.4
Structure-function studies on the mammalian intestinal dipeptide transporter hPEPT1 :: University of Southern California...
Intestinal protein digestion generates a massive variety and quantity of short chain peptides that are later absorbed into small intestinal epithelial cells by the di/tri-peptide transporter (PEPT1) on the apical membrane of enterocytes. PEPT1 functions as an electrogenic proton/peptide symporter with the ability to transport most di- and tripeptide arising from food digestion. It also has significance in its ability to transport pharmacologically active drugs. Due to its uniquely broad substrate specificity and high capacity, hPEPT1 has been suggested to be relevant drug targets at the level of drug transport. Substrate docking studies to identify specific interactions between the substrate and transporter would be facilitated by crystallization of hPEPT1; however, crystallization of hPEPT1 protein is currently unapproachable due to the size of the protein and the requirement of a lipid membrane to retain tertiary. My research built on the rudimentary computer model of hPEPT1 generated by Dr. ...http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/368654/rec/9
Structure-function studies on the mammalian intestinal dipeptide transporter hPEPT1 :: University of Southern California...
Intestinal protein digestion generates a massive variety and quantity of short chain peptides that are later absorbed into small intestinal epithelial cells by the di/tri-peptide transporter (PEPT1) on the apical membrane of enterocytes. PEPT1 functions as an electrogenic proton/peptide symporter with the ability to transport most di- and tripeptide arising from food digestion. It also has significance in its ability to transport pharmacologically active drugs. Due to its uniquely broad substrate specificity and high capacity, hPEPT1 has been suggested to be relevant drug targets at the level of drug transport. Substrate docking studies to identify specific interactions between the substrate and transporter would be facilitated by crystallization of hPEPT1; however, crystallization of hPEPT1 protein is currently unapproachable due to the size of the protein and the requirement of a lipid membrane to retain tertiary. My research built on the rudimentary computer model of hPEPT1 generated by Dr. ...http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/368654/rec/97
NIOSHTIC-2 Publications Search - 00232156 - HOCl causes airway substance P hyperresponsiveness and neutral endopeptidase...
Guinea-pig tracheal tissue was exposed to hypochlorous-acid (7790923) (HOCl) or hydrogen-peroxide (7722841) (H2O2) in an effort to determine whether there would be any effect on the responsiveness of airway muscle to acetylcholine (ACh), potassium-chloride (KCl), or substrate-P in the presence or absence of phosphoramidon. Tracheas were isolated from male Hartley-guinea-pigs, and used for airway chttps://www.cdc.gov/niosh/nioshtic-2/00232156.html
SLC46A2 Gene - GeneCards | TSCOT Protein | TSCOT Antibody
Complete information for SLC46A2 gene (Protein Coding), Solute Carrier Family 46 Member 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendiumhttp://www.genecards.org/cgi-bin/carddisp.pl?gene=SLC46A2