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*  Betulinic Acid-Induced Apoptosis in Glioma Cells: A Sequential Requirement for New Protein Synthesis, Formation of Reactive...
1995) Tumor suppressor p53 is a direct transcriptional activator of the human BAX gene. Cell 80:293-299. ... Transfer of TP53 can induce apoptosis in different cell types, including human glioma cells (Li et al., 1997). Evasion of the ... We find that BA-induced apoptosis of glioma cells is characterized by a sequential requirement for new protein, but not RNA, ... N-acetyl-Asp-Glu-Val-Asp-aldehyde. NAC. N-acetyl-cysteine. PARP. poly(ADP-ribose)polymerase. PBN. N-tert-butyl-α-phenylnitrone ...
  http://jpet.aspetjournals.org/content/289/3/1306.long
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Protoplasts are plant cells that have had their cell walls enzymatically removed. Isolation of protoplasts from different plant ... Co-expression of a viral RNA silencing suppressor, p23 or p19, in N. benthamiana resulted in earlier accumulation and increased ... Lignin, a polyphenolic material present in many cell wall types, is known to be a significant hindrance to enzyme access. ... Similar to Agrobacterium tumerfaciens, Agrobacterium rhizogenes can transfer foreign DNAs into plant cells based on the ...
  https://www.jove.com/visualize/abstract/25938231/manipulation-rice-l-galactose-pathway-evaluation-effects-transgene
*  Upcoming PhD Oral Examinations | Medical Biophysics, University of Toronto
Thesis Title: Function and regulation of the cell fate determinant Numb in polarized epithelial cells. Date & Time: Monday, ... Thesis Title: Structural and Biophysical Characterization of Tumor Suppressor p53-Interacting Proteins. Date & Time: Thursday, ... Val Lapin Thesis Title: RNAi Screening of the Kinome to Identify Mediators of Proliferation and Herceptin Resistance in HER2 ... Thesis Title: Functional analysis of the pseudo protease iRhom2 and nonsense-mediated RNA decay factor Smg1 using gene ...
  http://medbio.utoronto.ca/node/1843
*  JCI - TNF signaling drives myeloid-derived suppressor cell accumulation
Adoptive transfer of Tnfr+/+ CD11b+Gr1+ cells restores tumor growth in Tnfr-/- mice in a dose-dependent manner. (A and B) ... Real time RT-PCR. Total RNA was extracted from 1 × 107 isolated CD11b+Gr1+ cells with TRIzol (Invitrogen) and quantified on a ... 1 U enzyme activity was defined as the amount of enzyme that catalyzes the formation of 1 μmol of urea per minute. Data are ... 42% ± 3%; P , 0.01; Figure 4F). Addition of either the pan-caspase inhibitor Z-Val-Ala-Asp(OMe)-CH2F (z-VAD; 41% ± 2%) or the ...
  https://www.jci.org/articles/view/64115
*  humchr04.txt
FAT tumor suppressor homolog 4) (Fat-like cadherin protein FAT-J) [CDHF14] [FATJ] [Nbla00548] FBXL5 4p15.33 Q9UKA1 FBXL5_HUMAN ... RNA-binding motif protein 46) RBM47 4p14 A0AV96 RBM47_HUMAN RNA-binding protein 47 (RNA-binding motif protein 47) RBPJ 4p15.2 ... Ubiquitin-conjugating enzyme E2-25 kDa) (Ubiquitin-conjugating enzyme E2(25K)) (Ubiquitin-conjugating enzyme E2-25K) (Ubiquitin ... HUMAN 611434 Cytokine-dependent hematopoietic cell linker (Mast cell immunoreceptor signal transducer) [MIST] CLOCK 4q12 O15516 ...
  https://www.uniprot.org/docs/humchr04
*  Cell-Based Rna Interference and Related Methods and Compositions - Patent application
Dissecting p53 tumor suppressor functions in vivo. Cancer Cell 2002. 1: p. 289-98. [0126]Tumor cells which express exogenous ... RNA interference effects in the cells or their progeny. In certain embodiments, the system takes advantage of gene transfer of ... The term "encoded" is used to indicate that the vector, when acted upon by an appropriate enzyme, such as an RNA polymerase, ... the human and mouse H1 RNA promoter and the human tRNA-val promoter. A U6 snRNA leader sequence may be appended to the primary ...
  http://www.patentsencyclopedia.com/app/20080226553
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
This guided cell migration is referred as chemotaxis, which is essential for various cells to carry out their functions such as ... Co-expression of a viral RNA silencing suppressor, p23 or p19, in N. benthamiana resulted in earlier accumulation and increased ... The activity was measured by resting cell assays. For each oxidative step, enzyme activity was observed. To optimize the ... MitGO-ATeam is a Förster resonance energy transfer (FRET) probe in which the ε subunit of the FoF1-ATP synthase is sandwiched ...
  https://www.jove.com/visualize/abstract/25275379/cadmium-induced-hydrogen-sulfide-synthesis-is-involved-cadmium
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
CD4+ T cells, diabetogenic T cells, T cell transfer, diabetes induction method, diabetes, T cells, isolation, cell sorting, ... 8 Most anti-tumor gene therapies are focused on administration of the tumor suppressor genes, such as wild-type p53 (wt-p53), ... Co-expression of a viral RNA silencing suppressor, p23 or p19, in N. benthamiana resulted in earlier accumulation and increased ... We used a bi-functional caspase-3 substrate containing a DEVD (Asp-Glu-Val-Asp) caspase-3 recognition subunit and a DNA-binding ...
  https://www.jove.com/visualize/abstract/24466139/fuzi-attenuates-diabetic-neuropathy-rats-protects-schwann-cells-from
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Genes are commonly transfected into primary cells and cell lines in culture, however transfection of antibodies into cells has ... resulting in expression of mutated oncogenes and deletion of tumor suppressor genes. The phenotypic consequences of these ... It has been shown that patients with MS and HAM/TSP produce autoantibodies to the intracellular RNA binding protein ... Authors: Jason M. Conley, Tarsis F. Brust, Ruqiang Xu, Kevin D. Burris, Val J. Watts. ...
  https://www.jove.com/visualize/abstract/23284953/transport-mechanisms-their-pathology-induced-regulation-govern
*  Protocols and Video Articles Authored by Jianrong Li (Translated to Arabic)
In these reactions, a 5' diphosphate mRNA is capped by transfer of GMP via a covalent enzyme-GMP intermediate. RNA ... Here, we show that HN-independent fusion by L289A-F is not exhibited in BHK cells or in several other cell lines. However, ... Specifically, we show miR-204 may serve as a tumor suppressor gene at the 9q21.1-22.3 CAGR locus, a well established risk ... AP-F3 and NP-F1 but not Val-F1 significantly reduced meat lipid peroxidation by 20.1% and 12.9%, respectively. Our results ...
  https://www.jove.com/author/Jianrong_Li?language=Arabic
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... endothelial cells, cell signaling, cell, isolation, cell culture ... therapies are focused on administration of the tumor suppressor ... RNA interference), siRNA (small interfering RNA), cell-type specific delivery ... since extracellular addition of the enzyme promotes resealing of cells permeabilized in the absence of Ca2+. ... Briefly, translocation ability into lipid vesicles is measured as a function of the Foster Resonance Energy Transfer (FRET) ...
  https://www.jove.com/visualize/abstract/21998722/direct-translocation-as-major-cellular-uptake-for-cady-self
*  Index of genetics articles - Wikipedia
Skew Smooth endoplasmic reticulum SNP SnRNP Sociobiology Solenoid structure Somatic cell Somatic cell hybrid Somatic cells ... suppressor Noonan syndrome Norm of reaction Normal distribution Northern analysis Northern blot NPD Nu body Nuclear transfer ... protein Ribosomal RNA Ribosome Ribosomes Ribozyme Risk communication RNA RNA editing RNA phage RNA polymerase RNA replicase RNA ... Endonuclease Endopolyploidy Endosperm Enforced outbreeding Enhancer Enhancer trap Enriched medium Enucleate cell Enzyme ...
  https://en.wikipedia.org/wiki/Index_of_genetics_articles
*  Adenovirus‐Mediated Gene Transfer of Inhibitors of Apoptosis Proteins Delays Apoptosis in Cerebellar Granule Neurons, Journal...
Mediated Gene Transfer of Inhibitors of Apoptosis Proteins Delays Apoptosis in Cerebellar Granule Neurons, Journal of ... Adenovirus‐mediated gene transfer of the tumor suppressor, p53, induces apoptosis in postmitotic neurons. ... Bax deletion further orders the cell death pathway in cerebellar granule cells and suggests a caspase‐independent pathway to ... BAX‐induced cell death may not require interleukin 1β‐converting enzyme‐like proteases. ...
  https://www.deepdyve.com/lp/wiley/adenovirus-mediated-gene-transfer-of-inhibitors-of-apoptosis-proteins-ZJDDCz3sqA
*  Gene Report for G00002169 - Genes2Cognition Neuroscience Research Programme
The LKB1 tumor suppressor gene codes for a serine/threonine protein kinase, and among its substrates is the adenosine ... Some epithelial cells showed apical accumulation of LKB1, supporting its role in cell polarity. Regarding phospho-ACC (p-ACC), ... Two tryptic peptides derived from the enzyme become more radioactive after treatment of 32P-labelled cells with insulin. One of ... T1 is related to TC1, and has the structure: Ser-Ser(P)-Met-Ser-Gly-Leu-His-Leu-Val-Lys. C1 is identical with TC2. We have ...
  http://www.genes2cognition.org/db/Gene/G00002169
*  Gene Essay - avroarrow.org
BRAF is a protein kinase gene that is involved in putting out signals in cells that help produce cell division and growth. If ... 650 words - 3 pages Defining the Methyl-Specific Recognition of BRCT Domain Proteins An important tumor suppressor for ... During gene expression DNA is transcript to RNA and these RNA are transcribed to proteins. Bioinformatics deals with the ... The enzyme is also activated by various types of protein kinases, including protein kinase C (PKC) family members. On the other ...
  https://www.avroarrow.org/topic/gene
*  Gene Report for G00001322 - Genes2Cognition Neuroscience Research Programme
On blots of rat liver RNA, PMCA1 was more abundant than PMCA2, and purified rat parenchymal cell RNA gave similar findings. In ... Novel functional interaction between the plasma membrane Ca2+ pump 4b and the proapoptotic tumor suppressor Ras-associated ... Pretreatment of the cells with the caspase-3 inhibitor Z-DEVD-FMK (benzyloxycarbonyl-Asp(OMe)-Glu(OMe)-Val-Asp(OMe)- ... Although placental transfer of maternal calcium (Ca(2+)) is a crucial process for fetal development, the biochemical mechanisms ...
  http://www.genes2cognition.org/db/Gene/G00001322
*  Protocols and Video Articles Authored by Lijun Liu
A Model for Perineural Invasion in Head and Neck Squamous Cell Carcinoma' ... Isolation and Culture of Adult Mouse Cardiomyocytes for Cell Signaling and in vitro Cardiac Hypertrophy', ' ... to a lipid phosphatase that is similar to the tumor suppressor PTEN. How the VSD controls enzyme function has been unclear. ... which consequently inhibits cell proliferation in BT20 and DU145 cells. Consistently, we observe that small interfering RNA- ...
  https://www.jove.com/author/Lijun_Liu
*  Protocols and Video Articles Authored by Vitaly Citovsky
Assaying Proteasomal Degradation in a Cell-free System in Plants', 'Identification of Plasmodesmal Localization Sequences in ... A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment', 'Protein Membrane Overlay Assay: A ... from its tumor-inducing (Ti) plasmid into the host cell in a process known as horizontal gene transfer. Following its entry ... functions as an RNA-silencing suppressor that counteracts the innate immune response of the host plant. The host-cell target of ...
  https://www.jove.com/author/Vitaly_Citovsky
*  Organs of the immune system
follicular (FO) B cells. * T cell zones (TCZ) : T cells appear at pregnancy month 4. Here initial T and B cell activation ... IRTA1 was mainly expressed by tumor cells involved in lympho-epithelial lesions. In contrast, no or a low number of IRTA1+ ... It also improves and normalizes suppressor cell activities in diabetic patients. Thymosin-b4 can undergo enzymatic cleavage in ... Experiments with antisense thymosin-b15 RNA demonstrate that thymosin-b15 positively regulates cell motility, which has been ...
  http://www.ufrgs.br/imunovet/molecular_immunology/organsimmune.html
*  Activation of Vascular Endothelial Growth Factor Receptor-1 Sustains Angiogenesis and Bcl-2 Expression Via the...
Negative regulation of PKB/Akt-dependent cell survival by the tumor suppressor PTEN. Cell 95 : 29 -39,1998. ... Cells were treated with or without pan-caspase inhibitor z-Val-Ala-Dl-Asp-fluoromethylketone (z-VAD-fmk, 50 μmol/l) for 1 h ... Xiang J, Chao DT, Korsmeyer SJ: BAX-induced cell death may not require interleukin 1 beta-converting enzyme-like proteases. ... Our studies are consistent with the ability of adenoviral PlGF gene transfer studies in the ear of nude mice that resulted in ...
  http://diabetes.diabetesjournals.org/content/52/12/2959
*  Flavonoid Complexes as Promising Anticancer Metallodrugs | IntechOpen
... but the protective effect of cancer cells. The tumor cell lines studied after the application of modified zeolite CTQ or CTQD ... Chiral L/D‐valine (val) quercetin (Q) organotin(IV) complexes [(CH3)2Sn(Q)(val)] 1L/D [(C6H5)2Sn(Q)(val)] 2L/D. HeLa: IC50 (µg ... consecutively stimulating the tumorsuppressor protein p53, which consequently triggers cell apoptosis [69]. ... α and generate enzyme‐mediated DNA damage in cancer cells is an effective strategy in antitumor therapy [90]. Electrostatic ...
  https://www.intechopen.com/books/flavonoids-from-biosynthesis-to-human-health/flavonoid-complexes-as-promising-anticancer-metallodrugs/
*  Na+/2Cl-/K+ Cotransporter - Page 2 - BET-bromodomain inhibition research
... evading development suppressors, and sustaining proliferative signaling that enable cancer tumor cells to survive, proliferate ... RNA polymerase was packed into its capsid and ejected in to the sponsor cell thus removing the necessity to rely on sponsor RNA ... The enzyme tissue transglutaminase (tTG) has been recently identified to represent. Posted on June 17, 2017. by ... Passive transfer of less than 25 μL sensitized serum to naive supplementary recipients led to bone tissue marrow graft failing ...
  http://healthandwellnesssource.org/category/na2cl-k-cotransporter/page/2/
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... gene transfer, microRNA, overexpression, knock down, CD4 T cells, in vitro differentiation, regulatory T cell, virus, cell, ... miRNAs can function as oncogenes or tumor suppressors and are also promising biomarkers. Among the miRNA assays available, ... Bioluminescence resonance energy transfer (BRET) is a method similar to FRET except the donor is an enzyme (e.g. luciferase) ... In the cytoplasm, the PRRs RIG-I and PKR bind to specific viral RNA ligands. This first mediates conformational switching and ...
  https://www.jove.com/visualize/abstract/23342057/ack1-activation-and-regulation-by-allostery
*  Protocols and Video Articles Authored by Yao Zhang (Translated to Russian)
Sep, 2009 , Pubmed ID: 19551515 PDCD5 (programmed cell death 5) accelerates apoptosis of certain tumor cells and the ... MARVELD1-overexpressing cells were trasfected with p53 or p16 siRNA.Our findings suggest that MARVELD1 is a tumor suppressor by ... These results may suggest that negative pressure cavitation can compel heavy metals to transfer from the cell surface into ... by silencing PLCε with short hairpin RNA (shRNA) in human bladder cancer cells BIU-87 in vitro and in vivo. ...
  https://www.jove.com/author/Yao_Zhang?language=Russian
*  Compositions and methods relating to novel compounds and targets thereof - Glick, Gary D.
... different tumorigenic cancer cell lines with nontumorigenic lines show consistently lower profilin 1 levels in tumor cells. ... Expression profiling using RNA isolated from cells treated with Bz-423, Bz-OMe, or vehicle control was done with the HGU133A ... The synthesis of poylamines in cells involves several enzymatic steps, however ornithine decarboxylase is the enzyme that ... "Gene transfer vectors for mammalian cells" (J. M. Miller & M. P. Calos, eds., 1987); "Current protocols in molecular biology" ( ...
  http://www.freepatentsonline.com/y2006/0025388.html