Measurement of sulfobromophthalein uptake in isolated rat hepatocytes by a direct spectrophotometric method. | Archivio della...
A spectrophotometric technique is described for the continuous recording of sulfobromophthalein uptake by isolated hepatocytes. The technique is based on the principle that sulfobromophthalein behaves as a pH-indicator and may be followed photometrically when moving from the medium at pH 7.8 into the interior of the cell. Data show that upon addition of cells to a sulfobromophthalein solution, an absorbance change can be recorded. The kinetics of the process is biphasic and the initial rate is linearly related to the amount of cells added. By this technique it was confirmed that the substrate dependence of the initial velocity of transport is a compound function including a saturable portion with an apparent Km in the mu molar region. Experiments carried out either in the presence of valinomycin or of high concentrations of potassium chloride indicate that the presence of a membrane potential opposes the entry of sulfobromophthalein into ...https://www.iris.unisa.it/handle/11386/3138247
Differences of re absorption of unconjugated sulfobromophthalein sodium and sulfobromophthalein sodium glutathione from the rat...
Dammann, H.G.; Czok, G., 1975: Differences of re absorption of unconjugated sulfobromophthalein sodium and sulfobromophthalein sodium glutathione from the rat biliary tree after retrograde intra biliary injectionhttps://eurekamag.com/research/005/151/005151802.php
bromsulphthalein | Ligand page | IUPHAR/BPS Guide to PHARMACOLOGY
The IUPHAR/BPS Guide to Pharmacology. bromsulphthalein ligand page. Quantitative data and detailed annnotation of the targets of licensed and experimental drugs.http://www.guidetopharmacology.org/GRAC/LigandDisplayForward?tab=summary&ligandId=4506
Expression of sodium-independent organic anion uptake systems of skate liver in Xenopus laevis oocytes - edoc
The expression of the basolateral sodium-independent organic anion uptake system of the little skate (Raja erinacea) has been studied in Xenopus laevis oocytes. Injection of oocytes with skate liver poly(A)+ RNA resulted in the functional expression of chloride-dependent sulfobromophthalein (BSP) uptake and sodium-independent taurocholate uptake within 3-5 days. The expressed chloride-dependent BSP uptake activity exhibited saturation kinetics [apparent Michaelis constant (Km) 1.8 microM] and efficiently extracted BSP from its binding sites on bovine serum albumin. The chloride-sensitive portion of BSP uptake was inhibited by bilirubin (10 microM; 27%), 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (100 microM; 57%), bumetanide (100 microM; 48%), taurocholate (200 microM; 51%), and cholate (200 microM; 45%). Size fractionation of total skate liver mRNA revealed that a 1.8- to 2.9-kb size class mRNA was sufficient to express chloride-dependent BSP uptake ...http://edoc.unibas.ch/3739/
Our study demonstrates that the antihyperlipidemic clofibrate derivatives, which are structurally different from the known activator, sulfobromophthalein, exert stimulatory effects specifically on AKR 1C4 of human liver 3αHSD isoforms. For the activation by sulfobromophthalein, there has been no direct information about the structurally specific interaction between the molecule and the enzyme, except for its sulfonyl group(s) (Matsuura et al., 1996,1997). Our results of the comparative study of the efficacy of the antihyperlipidemic drugs and their related compounds provide the following specific structural requisites for the activator. 1) The existence of a negatively charged carboxyl group, together with at least a hydrophobic aromatic ring, in the activator molecule is necessary to interact with the activator site of the enzyme, because the clofibric acid derivatives lacking the free ...http://jpet.aspetjournals.org/content/285/3/1096
The hepatic transport of organic anions has been studied extensively. In rats, sinusoidal uptake of many organic anions is mediated by the oatp proteins (oatp1 and oatp2) whose substrates include bile acids as well as nonbile acid organic anions (Meier et al., 1997; Muller and Jansen, 1998). Additionally, at least three other carrier systems may mediate sinusoidal uptake of nonbile acid organic anions, including another family of multispecific transporters (oat) (Sekine et al., 1998), bilitranslocase and bromosulfophthalein/bilirubin-binding protein (Meier et al., 1997). Sinusoidal efflux of organic anions from the liver also has been shown to be carrier-mediated (De Vries et al., 1985; Evans et al., 1995). In this study, the appearance in perfusate of acetaminophen glucuronide and clofibric acid glucuronide during perfusions with the respective parent aglycones (Fig. 1) demonstrates sinusoidal efflux of hepatically generated ether and acyl glucuronides. Although the identity of the efflux ...http://jpet.aspetjournals.org/content/295/1/44
Ochratoxin A (OTA) is a mycotoxin produced by mold. It mainly causes nephropathies in humans and domestic animals as a major pathogenic contaminant of cereals and animal feed. Upon p.o. uptake and intestinal absorption, a large part of OTA is taken up by hepatocytes and eliminated into bile. In the present study, hepatocellular uptake of radiolabeled [3H]OTA in isolated rat hepatocytes was characterized; a saturable (K(m) = 18.9 microM, Vmax = 473 pmol/mg/min), temperature (Aapp = 30.4 and 76.6 kilo Joule/mol) and energy-dependent mycotoxin transport was found. This OTA uptake was inhibited by various bile acids, sulfobromophthalein and the thrombin inhibitor CRC 220. Because all inhibitors are substrates of the organic anion-transporting polypeptide (oatp), a recently cloned hepatic carrier, uptake experiments were performed in oatp-cRNA-injected Xenopus laevis oocytes. These studies revealed an oatp-specific OTA uptake (K(m) = 16.6 microM). In contrast, OTA was not transported by the ...http://jpet.aspetjournals.org/content/279/3/1507
Liver Disease in Crohn's ColitisA Study of 21 Consecutive Patients Having Colectomy | Annals of Internal Medicine | American...
Twenty-one consecutive patients undergoing colectomy for Crohn's disease of total colon were studied, with particular emphasis on hepatic abnormality at colectomy and at 3- to 7-year follow-up. After colectomy there was a high incidence of recurrent Crohn's disease involving small bowel, regardless of initial small-bowel involvement. There were abnormalities of liver histology in 90% of the 20 operative biopsies, with 75% showing more than minor change. Cirrhosis, amyloidosis, and multiple hepatic granulomata were also noted. Elevated serum alkaline phosphatase was of value in detecting significant hepatic abnormality. Other tests were not as useful, being either too insensitive (such as serum bilirubin and serum transaminases) or too nonspecific (serum protein abnormalities and sulfobromophthalein retention). Blood transfusions, potentially hepatotoxic drugs, and previous hepatitis appeared unrelated to observed hepatic abnormalities. The frequency and type of hepatic abnormality ...http://annals.org/aim/article-abstract/685299/liver-disease-crohn-s-colitis-study-21-consecutive-patients-having
Changes of laboratory variables with time in cirrhosis: Prognostic and therapeutic significance - Christensen - 1985 -...
The time change of laboratory variables in cirrhosis was studied by analysis of data from 488 patients with cirrhosis included in a controlled clinical trial of long-term prednisone vs. placebo.. In the placebo group, a marked regression towards normal was seen within 3 months of entry into the trial (increase in serum albumin, acetylcholinesterase, cholesterol, hemoglobin and decrease in erythrocyte sedimentation rate). The subsequent course did not show a clear pattern, except for a slight increase in serum bilirubin and decrease in albumin. When studied in relation to the time of death in patients dying from a "hepatic" cause, marked increase in bilirubin and decrease in prothrombin index, albumin and cholesterol were seen in the year prior to death with little change before that time.. In the prednisone group, a more marked decrease in bilirubin, SGOT, alkaline phosphatase, γ-globulin, sulfobromophthalein retention, erythrocyte sedimentation rate and increase in leukocytes, prothrombin ...http://onlinelibrary.wiley.com/doi/10.1002/hep.1840050523/abstract
Idiopathic hepatic fibrosis in 15 dogs | Veterinary Record
Idiopathic hepatic fibrosis was diagnosed by liver biopsy in 15 young dogs, of which nine were German shepherds. Clinical signs included ascites, anorexia, weight loss and hepatic encephalopathy. Erythrocyte microcytosis was a consistent clinical feature, and clinical chemistry generally revealed hypoproteinaemia and high serum activities of alkaline phosphatase and, to a smaller extent, alanine aminotransferase. Fasting blood ammonia and serum bile acid concentrations were increased in most dogs examined, and all the dogs tested had prolonged retention of sulfobromophthalein at 30 minutes. Multiple acquired portosystemic shunts were revealed by laparotomy and/or portography. Non-inflammatory fibrosis was present to different degrees in all the dogs' livers, and on the basis of its predominant location these were classified as having central perivenous fibrosis, diffuse pericellular fibrosis or periportal fibrosis. The response to symptomatic treatment and anti-fibrotic therapy with ...http://veterinaryrecord.bmj.com/content/133/5/115
JCI - Welcome
In the 1960s, my lab was interested in understanding how bilirubin and other organic anions are transferred from the plasma through the liver cell and into the bile. We performed gel filtration of liver supernatants and identified two protein fractions, designated Y and Z, which bound organic anions including bilirubin, and thus we proposed that they were involved in hepatic uptake of organic anions from plasma. Subsequently, the Y and Z proteins responsible for this binding activity were purified, cloned, and sequenced. Y was identified as a member of the glutathione S-transferase (GST) protein family and Z found to be a member of the fatty acid-binding protein (FABP) family. These proteins have since been shown to have additional surprising roles, but understanding of their full role in physiology and disease has not yet been achieved.. ...https://www.jci.org/tags/92?content=articles&page=3
5508 Organic anion transporting polypeptides (OATPs) are large a family of membrane transporters which transport steroid sulfates and other organic anions across cell membranes. OATP2B1 (also known as OATP-B) is one member of this family which is known to transport estrone sulfate (E1S). E1S is the main reservoir of steroid hormones, forming biologically active estrogen via the action of steroid sulfatase (STS). The higher circulating levels of E1S in postmenopausal women, higher STS mRNA expression in breast tumour tissues and higher intra-tumoral STS activity suggest that functions of uptake transporters are important in hormone-dependent cancers. In the present study we have investigated the role of OATP2B1 in governing E1S transport and STS activity. Cellular uptake of E1S and corresponding STS activities were measured in 293-EBNA cells before and after transient transfection with OATP2B1 cDNA (293-EBNA[OATP]). Transport assays were carried out using 5-6 nM of 3H E1S and the amount of 3H ...http://cancerres.aacrjournals.org/content/66/8_Supplement/1295.2
Cartilage and bone macromolecules in knee joint synovial fluid in rheumatoid arthritis: relation to development of knee or hip...
The aggrecan concentrations as well as the aggrecan/COMP ratios decreased during progression of the destruction in the destructive group, which corroborates previous results from cross sectional studies.17 18 Thus we find striking changes in the release of aggrecan during development of destruction, whereas the COMP levels seem to vary little. This contrasts findings in a cross sectional study, where slightly, but significantly, lower concentrations of COMP were seen in patients with advanced destruction.19 The reasons for this discrepancy is at present unclear. The BSP concentrations in the destructive group increased during progression of destruction, which corroborates and extends the observations from our previous cross sectional study.22 It should be noted that the concentrations of BSP did not differ between the groups at initiation of the study and no clinical usefulness of BSP in predicting individual susceptibility is therefore apparent.. The longitudinal changes of the synovial fluid ...http://ard.bmj.com/content/56/2/91
Monoclonal Gammopathy of Undetermined Significance: Genetic vs Environmental Etiologies
Serum bone sialoprotein as a marker of tumour burden and neoplastic bone involvement and as a prognostic factor in multiple myeloma. Woitge, H W; Pecherstorfer, M; Horn, E; Keck, A-V; Diel, I J; Bayer, P; Ludwig, H; Ziegler, R; Seibel, M J // British Journal of Cancer;2/1/2001, Vol. 84 Issue 3, p344 To test the potential of immunoreactive BSP, a non-collagenous bone matrix component, as a clinical guide in patients with plasma cell dyscrasias, serum BSP concentrations were measured in 62 patients with newly diagnosed multiple myeloma (MM) followed over a period of 4 years, in 46 patients... ...http://connection.ebscohost.com/c/articles/27828546/monoclonal-gammopathy-undetermined-significance-genetic-vs-environmental-etiologies
Microvillous membrane vesicles from the term human placental syncytiotrophoblast were used to characterize further the properties of a transport mechanism for bile acids. Taurocholate (TC) uptake into an osmotically reactive intravesicular space was temperature dependent and independent of sodium. TC uptake (2 microM) was markedly inhibited by 250 microM taurine and glycine-conjugated cholate and chenodeoxycholate and unconjugated cholate but not by chenodeoxycholate, deoxycholate, etianic acid, bromosulfophthalein, pyruvate, lactate, alanine, or taurine. The initial rate of TC uptake was inhibited significantly by the anion transport inhibitor 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) but was not inhibited significantly by 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid, amiloride, or furosemide. Preincubation of vesicles with DIDS in the presence of TC partially blocked the action of the inhibitor. Efflux of 5 microM TC from membrane vesicles was stimulated by the ...http://ajpgi.physiology.org/content/264/6/G1016
Sodium p-aminohippurate - Diagnostic aids
Aminohippurate (p-aminohippuric acid, PAH, PAHA) is the glycine amide of p-aminobenzoic acid. It is filtered by the glomeruli and is actively secreted by the proximal tubules. At low plasma concentrations (1.0 to 2.0 mg/100 mL), an average of 90 percent of aminohippurate is cleared by the kidneys from the renal blood stream in a single circulation. It is ideally suited for measurement of ERPF since it has a high clearance, is essentially nontoxic at the plasma concentrations reached with recommended doses, and its analytical determination is relatively simple and accurate. Aminohippurate is also used to measure the functional capacity of the renal tubular secretory mechanism or transport maximum (TmPAH). This is accomplished by elevating the plasma concentration to levels (40-60 mg/100 mL) sufficient to saturate the maximal capacity of the tubular cells to secrete aminohippurate. Inulin clearance is generally measured during TmPAH determinations since glomerular filtration rate (GFR) must be ...http://pharmacycode.com/Sodium_p-aminohippurate.html
A Paravascular Pathway Facilitates CSF Flow Through the Brain Parenchyma and the Clearance of Interstitial Solutes, Including...
Because it lacks a lymphatic circulation, the brain must clear extracellular proteins by an alternative mechanism. The cerebrospinal fluid (CSF) functions as a sink for brain extracellular solutes, but it is not clear how solutes from the brain interstitium move from the parenchyma to the CSF. We demonstrate that a substantial portion of subarachnoid CSF cycles through the brain interstitial space. On the basis of in vivo two-photon imaging of small fluorescent tracers, we showed that CSF enters the parenchyma along paravascular spaces that surround penetrating arteries and that brain interstitial fluid is cleared along paravenous drainage pathways. Animals lacking the water channel aquaporin-4 (AQP4) in astrocytes exhibit slowed CSF influx through this system and a ~70% reduction in interstitial solute clearance, suggesting that the bulk fluid flow between these anatomical influx and efflux routes is supported by astrocytic water transport. Fluorescent-tagged amyloid β, a peptide thought to be ...http://stm.sciencemag.org/content/4/147/147ra111
Molecular alterations of canalicular transport systems in experimental models of cholestasis: possible functional correlations.
The discovery of unidirectional, ATP-dependent canalicular transport systems (also termed 'export pumps') for bile salts, amphiphilic anionic conjugates, lipophilic cations, and phospholipids has opened new opportunities for understanding biliary phyhttp://www.biomedsearch.com/nih/Molecular-alterations-canalicular-transport-systems/9626757.html
Coproporphyrine Isomers and Methotrexate Elimination - Full Text View - ClinicalTrials.gov
MTX is a substrate of MRP2, a renal tubular transporter encoded by the ABCC2 gene. It has been shown that single nucleotide polymorphisms (SNPs) on the ABCC2 gene are associated with impairment of MTX elimination. Mutations on the ABCC2 gene are also responsible for the Dubin-Johnson syndrome, characterised by the absence of a functional MRP2 protein. Apart from hyperbilirubinaemia, the main biological perturbation observed in this disease is a typical increase of the urinary ratio of coproporphyrins I (I+ III) (UCP ratio). Our hypothesis is that the UCP ratio could be used as a biomarker of MRP2's activity, thus predicting MTX elimination. One hundred patients treated with high dose MTX will be recruited in this prospective study. Their UCP ratio will be measured before and after MTX administration and correlated with MTX clearance. A genetic analysis will be conducted to study the five more frequents SNPs of ABCC2 in each patient ...https://clinicaltrials.gov/ct2/show/NCT00822432
Gadoxetate Sodium - Drugs.com
Gadoxetate Sodium is a medicine available in a number of countries worldwide. A list of US medications equivalent to Gadoxetate Sodium is available on the Drugs.com website.https://www.drugs.com/international/gadoxetate-sodium.html
Relation between indocyanine green (ICG) plasma disappearance rate and ICG blood clearance in critically ill patients |...
In contrast to indocyanine green (ICG) blood clearance, the plasma disappearance rate (PDR) of ICG does not require absolute ICG blood concentrations and today can be assessed transcutaneously. In thihttps://link.springer.com/article/10.1007%2Fs00134-006-0109-6
Fimbriae Y protein elisa and antibody
Shop Fimbriae Y protein ELISA Kit, Recombinant Protein and Fimbriae Y protein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.https://www.mybiosource.com/protein_family.php?root=fimbriae-y-protein
Gadoxetate Disodium Enhanced MRI of the Liver: Part 2, Protocol Optimization and Lesion Appearance in the Cirrhotic Liver - PDF
Gastrointestinal Imaging Review Cruite et al. Contrast-Enhanced MRI in Liver Cirrhosis Gastrointestinal Imaging Review FOCUS ON: Gadoxetate Disodium Enhanced MRI of the Liver: Part 2, Protocol Optimizationhttp://docplayer.net/254823-Gadoxetate-disodium-enhanced-mri-of-the-liver-part-2-protocol-optimization-and-lesion-appearance-in-the-cirrhotic-liver.html
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Gentaur Molecular :BioBasic \ Column Ni NTA Agarose \ BSP079-1
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