Frontiers | Therapeutic Antibodies against Intracellular Tumor Antigens | Immunology
Advances in the production of TCRm antibodies have enabled the generation of multiple TCRm antibodies, which have been tested ... Advances in the production of TCRm antibodies have enabled the generation of multiple TCRm antibodies, which have been tested ... Antibodies have been developed to target externalized antigens, have also been engineered to enter into cells or may be ... Antibodies have been developed to target externalized antigens, have also been engineered to enter into cells or may be ...https://www.frontiersin.org/articles/10.3389/fimmu.2017.01001/full
Application # 2016/0194410. BINDING AGENT - Patents.com
... domains, single domain antibodies (see: Hey, T. and Fiedler, E., et al., Trends Biotechnol. 23 (2005) 514-522), and monovalent ... selected to have different specificity. The binding pairs a:a', b:b' and the binding pair to which X is bound each bind to (e.g ... an antibody. In certain embodiments, a single-domain antibody is a human single-domain antibody (Domantis, Inc., Waltham, Mass ... disclosure is a single-domain antibody. A single-domain antibody is a single polypeptide chain comprising all or a portion of ...http://patents.com/us-20160194410.html
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Single-domain antibodies (sdAbs) derived from human V(H) are considered to be less soluble and prone to aggregate which makes ... FIPA analysis also introduces the option to bind more than one differently tagged AFP on the same single ice crystal to help ... The AFP-bound ice hemisphere is imaged under UV light to visualize AFP-bound planes using filters to block out nonspecific ... Selected sequences are then run through a fold specificity stage and a binding affinity stage. A rank-ordered list of the ...https://www.jove.com/visualize/abstract/22253912/crystal-structure-human-single-domain-antibody-dimer-formed-through-v
Self-organization of keratin intermediate filaments into cross-linked networks | JCB
... single-domain swap) chimeras, and of the K5bd and K8bc (dual domain swap) chimeras. (f) Testing the binding of GFP-T14 to each ... Binding was detected with rabbit anti-GFP and HRP-conjugated goat anti-rabbit antibodies. (b) Other type II keratins also bind ... We next sought to define the regions of K5 bound by K14's tail domain. Experiments involving BNPS-Skatole, which chemically ... further the evidence showing that the rod-proximal head and L2-2B domains in K5 both contribute to K14's tail domain binding. ...http://jcb.rupress.org/content/186/3/409
Frontiers | Intracellular Crosslinking of Filoviral Nucleoproteins with Xintrabodies Restricts Viral Packaging | Immunology
By retuning sdAb specificity, the approach of crosslinking highly conserved regions of assembly critical proteins may well be ... likely benefit from transitioning away from intracellular expression of crosslinking sdAb to exogenous delivery of antibody. ... Previously, we had generated sdAb (single-domain antibodies) against Filoviruses that have all targeted highly conserved C- ... Previously, we had generated sdAb (single-domain antibodies) against Filoviruses that have all targeted highly conserved C- ...https://www.frontiersin.org/articles/10.3389/fimmu.2017.01197/full
Frontiers | Fc Engineering for Developing Therapeutic Bispecific Antibodies and Novel Scaffolds | Immunology
Promisingly, Fc antigen binding fragment [Fcab] and monomeric antibody or half antibody may be particularly advantageous to ... Promisingly, Fc antigen binding fragment [Fcab] and monomeric antibody or half antibody may be particularly advantageous to ... to antibody fragments. Impressively, antibody fragments such as bispecific T-cell engager [BiTE], bispecific killer cell ... to antibody fragments. Impressively, antibody fragments such as bispecific T-cell engager [BiTE], bispecific killer cell ...https://www.frontiersin.org/articles/10.3389/fimmu.2017.00038/full
IJMS | Free Full-Text | Bispecific Antibodies as a Development Platform for New Concepts and Treatment Strategies | HTML
Bispecific antibodies that can simultaneously bind to cell surface antigens and payloads are a very ideal delivery system for ... The single-chain-based bispecific antibodies usually bridge tumor cells with immune cells and form an immunological synapse ... Bispecific antibodies in the IgG format include asymmetric bispecific antibodies and homodimerized bispecific antibodies, all ... Bispecific antibodies can also be used to treat hemophilia A by mimicking the function of factor VIII. Bispecific antibodies ...http://www.mdpi.com/1422-0067/18/1/48/htm
A cooperative-binding split aptamer assay for rapid, specific and ultra-sensitive fluorescence detection of cocaine in saliva ...
20 based on single-domain cocaine-binding split aptamers, demonstrating that CBSA retains excellent specificity for its target ... in which either of the two binding domains was disrupted by this single-nucleotide mutation, leaving only a single binding ... 2 which can specifically bind to a wide range of targets including proteins, small molecules and metal ions.3 Aptamers offer ... this fluorescence is greatly quenched when ATMND is bound to a DNA duplex in this fashion.33 We therefore replaced the ...http://pubs.rsc.org/-/content/articlehtml/2017/sc/c6sc01833e
Transglutaminase for inhibiting angiogenesis - Patent # 7704943 - PatentGenius
USA 85, 5879) and single domain antibodies (dAbs) comprising isolated V domains (Ward et at (1989) Nature341, 544). A general ... The antibodies described in Table 2 are generally available from Serotec, Oxford, OX5 1BR, UK.. Monoclonal antibodies that bind ... Monoclonal antibodies directed against the .alpha..sub.v.beta..sub.3integrin (LM609) have been covalently bound to paramagnetic ... In combining two or more binding sites, which in themselves may be of high specificity but low affinity, it will be possible to ...http://www.patentgenius.com/patent/7704943.html
Rational design of antibodies targeting specific epitopes within intrinsically disordered proteins | PNAS
We illustrate the method by designing six single-domain antibodies to bind different epitopes within three disease-related ... We have shown that this method generates antibodies that can bind with good specificity and affinity target regions in three ... and IAPP bound to DesAb-F was estimated measuring the absorbance at 492 nm after verifying that the primary antibodies ... 2008) Comprehensive analysis of the factors contributing to the stability and solubility of autonomous human VH domains. J Biol ...http://www.pnas.org/content/112/32/9902
Durable antitumor responses to CD47 blockade require adaptive immune stimulation | PNAS
Here, we use a novel anti-CD47 single domain antibody, derived from an alpaca, in an immunocompetent mouse model of melanoma ... Anti-CD47 Nanobodies Bind with High Affinity and Specificity to Mouse CD47.. To further evaluate the binding properties of the ... Bound mouse antibody was detected using an anti-IgG conjugated to HRP (Southern Biotech). (B) Mice were injected i.p. with 200 ... and can be readily reformatted as a genetic fusion to alternative protein domains, making them ideal therapeutic and research ...http://www.pnas.org/content/113/19/E2646
A Human Single-Domain Antibody Elicits Potent Antitumor Activity by Targeting an Epitope in Mesothelin Close to the Cancer Cell...
It moderately bound CCA cell line (Mz-ChA-1) and weakly bound human NSCLC cell lines (EKVX and L55). It did not bind to ... we also constructed VH single-domain molecules based on SS1 as well as HN1. We expressed and compared the VH domains of SD1, ... Antibody specificity controls in vivo effector mechanisms of anti-CD20 reagents. Blood 2004;103:2738-43. ... Screening an engineered human antibody domain library. An engineered human (VH) antibody domain library named m8l showed an ...http://mct.aacrjournals.org/content/12/4/416?rss=1
Discovery Biology News
In a report by Tereshko et al., camelid single domain antibodies, specifically the variable heavy chain (VHH) domains can be ... Single domain antibodies have the advantage of expedited optimization, flexibility of incorporating multiple specificity and ... One of the challenges, particularly with membrane bound proteins and transmembrane receptors, is the ability to obtain a well ... which encodes a protein required for polymerase II function and shown previously to bind to triptolide. From the screen, the ...https://www.genscript.com/DiscoveryBiology_news.html
US5055289A - Interferon antibody therapeutic compositions having an extended serum half-life - Google Patents
A complex of alpha-interferon with a monoclonal antibody complexes with the alpha-interferon without impairing its antiviral ... antibody variable domains and antagonists EP2316852A1 (en) 2002-11-08. 2011-05-04. Ablynx N.V.. Stabilized single domain ... the other specificity could be selected to bind with an agent which has anti-tumor activity. For example, the second ... the reaction mixtures were adsorbed with solid phase sepharose bound sheep anti-mouse IgG to remove the antibody and complexed ...https://patents.google.com/patent/US5055289?oq=flatulence
Protocols and Video Articles Authored by Victor A. Streltsov
... are unique single domain antibodies found in the serum of sharks. The individual variable (VNAR) domains bind antigen ... high specificity binding reagents. From a pool of monoclonal antibodies, we selected one such candidate (A4D11) with high ... Zanamivir and peramivir bound slowly to the wild type NA, but binding of oseltamivir was more rapid. The D197E/N mutations ... to create a diverse library of single domain antibody fragments based on the shark IgNAR antibody isotype. Coupling of these ...https://www.jove.com/author/Victor+A._Streltsov
IJMS | Free Full-Text | Antibody Phage Display Libraries: Contributions to Oncology | HTML
Examples of such antibodies will be discussed here with emphasis on those used as probes for molecular imaging and other ... Phage display represents a high-throughput technique for screening billions of random fusion antibodies against virtually any ... Robust techniques combining an antibody library displayed on the phage surface and protein microarray allowed the ... antibody libraries displayed on filamentous phage surfaces have been used to identify specific binders for many different ...http://www.mdpi.com/1422-0067/13/5/5420/htm
Antibodies | Free Full-Text | Single Domain Antibody Fragments as Drug Surrogates Targeting Protein-Protein Interactions...
In this review, we discuss our work on single domains as intracellular antibodies and where this work might in the future. ... Our recent technology development has provided a set of simple protocols that allow development of single antibody variable (V ... Antibody fragments can be used for inhibiting PPI. ... region domains that can function inside the reducing ... the anti-RAS VH does not bind to GDP-bound, inactive RAS. The massive difference in the interaction strength of the VH with RAS ...http://mdpi.com/2073-4468/2/2/306/htm
Mapping autoantigen epitopes: molecular insights into autoantibody-associated disorders of the nervous system | Journal of...
... like domain. Anti-MuSK antibodies bind to the three N-linked Ig-like domains and the Fz-like domain [83-85]. d The neuronal P/Q ... recent reevaluation of autoantibody specificity against VGKC by cell-based assays has demonstrated that bound antigens were in ... with the Disc domain containing the major target epitope. Indeed, the ΔDisc single domain deletion construct was the only one ... and Mucin-like domain. Anti-NF155 antibodies target extracellular domains Fn III (or Fn3) and Fn IV (Fn4) . c Antibodies ...https://jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-016-0678-4
Frontiers | Extensive Basal Level Activation of Complement Mannose-Binding Lectin-Associated Serine Protease-3: Kinetic...
All MASPs have the same six-domain structure (4, 7). The C-terminal SP domain is preceded by five regulatory domains in the ... or a MASP-3-specific antibody. Both antibodies were developed against the serine protease (SP) domain of the corresponding ... Factor D (FD), a single-domain SP, is a key enzyme for the alternative pathway. FD has only one natural substrate, factor B (FB ... MASPs are expected to bind preferably to MBL-Sepharose as a result of the 3 to 1, immobilized rMBL to soluble PRM ratio. After ...https://www.frontiersin.org/articles/10.3389/fimmu.2017.01821/full
William Weis | Stanford Medicine Profiles
Here we report stabilization of two distinct β2AR conformations using single domain camelid antibodies (nanobodies)-a ... We demonstrate that these regions form discrete subdomains that bind to IFs and report the crystal structures of domains B and ... We present the crystal structure of the Dkk1 C-terminal domain bound to LRP6(3-4), and show that the Dkk1 N-terminal domain ... the much narrower binding specificity of selectin cell adhesion molecules results from an extended binding site within a single ...https://med.stanford.edu/profiles/william-weis
Scientific Protocols - Engineering of ultra-small diagnostic nanoprobes through oriented conjugation of single-domain...
Holliger, P. & Hudson, P.J. Engineered antibody fragments and the rise of single domains. Nat. Biotechnol. 23, 1126-1136 (2005 ... An sdAb molecule occupies 12 times less space than a conventional IgG antibody does, and its size allows it to bind epitopes ... and diagnosis with nanoparticles is to make use of the specificity and avidity of monoclonal antibodies (mAbs) for targeting ( ... Other known strategies for Ab covalent coupling to QDs require a passivating ligand bound to the quantum surface to make it ...https://protocols.scienceexchange.com/protocols/engineering-of-ultra-small-diagnostic-nanoprobes-through-oriented-conjugation-of-single-domain-antibodies-and-quantum-dots
Chimeric camel/human heavy-chain antibodies protect against MERS-CoV infection | Science Advances
Nanobodies: Natural single-domain antibodies. Annu. Rev. Biochem. 82, 775-797 (2013).. ... bound to all variants (Fig. 4, A and B), whereas the four MERS-CoV-specific VHHs did not bind to the D539N variant and differed ... To confirm the specificity of the VHHs for MERS-CoV, we randomly selected several clones for double staining of MERS-CoV- ... 6A), consistent with the reported rapid clearance of small VHH domains from the circulation (31). Significant levels of ...http://advances.sciencemag.org/content/4/8/eaas9667.full
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
However, as another opportunity, these interesting and stable single-domain antibodies8 can be easily engineered with different ... Both the usefulness and limitation of the method depends on whether the antibodies employed are able to bind to native epitopes ... When the lipid vesicles are incubated with a cell lysate the protein binding to the target lipid will also be bound to the ... The hydrophobic transmembrane domains make the proteins insoluble in aqueous buffer. Before a transmembrane protein can be ...https://www.jove.com/visualize/abstract/25811612/inhibition-of-type-vi-secretion-by-an-anti-tssm-llama-nanobody
TWO-DIMENSIONAL PHOTONIC BANDGAP STRUCTURES FOR ULTRAHIGH-SENSITIVITY BIOSENSING - Patent application
... single-chain antibodies (i.e., covalently linked variable heavy (VH) and light (VL) domains), single-domain antibodies (i.e., ... single proteins, single copies of nucleic acids) penetrate the defect but (even if nonspecifically bound) do not provide ... Patent applications in class By measuring the ability to specifically bind a target molecule (e.g., antibody-antigen binding, ... 0141]The specificity and selectivity of these coupled-cavity devices will be tested by exposing the sensor to a solution ...http://www.patentsencyclopedia.com/app/20100279886
Molecular characterization and serodiagnostic potential of a novel dithiol glutaredoxin 1 from Echinococcus granulosus |...
An iELISA using this antigen showed specificity of 64.3 % (9/14) and sensitivity of 1:3200, and the diagnostic accordance rate ... We cloned and expressed recombinant Eg-Grx1 and generated antibodies. We analyzed the location of the protein in different ... Grxs can be further categorized into multi-domain dithiol Grxs consisting of two or four dithiol Grx domains and single-domain ... and was classified as a single-domain dithiol Grx.. Grxs are defined by their ability to bind and utilize GSH as substrate [17 ...https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1741-9