*  Angiotensin-(1-7) decreases skeletal muscle atrophy induced by angiotensin II through a Mas receptor-dependent mechanism |...
Skeletal muscle atrophy is a pathological condition characterized by the loss of strength and muscle mass, an increase in myosin heavy chain (MHC) degradation and increase in the expression of two muscle-specific ubiquitin ligases: atrogin-1 and MuRF-1. Angiotensin II (AngII) induces muscle atrophy. Angiotensin-(1-7) [Ang-(1-7)], through its receptor Mas, produces the opposite effects than AngII. We assessed the effects of Ang-(1-7) on the skeletal muscle atrophy induced by AngII. Our results show that Ang-(1-7), through Mas, prevents the effects induced by AngII in muscle gastrocnemius: the decrease in the fibre diameter, muscle strength and MHC levels and the increase in atrogin-1 and MuRF-1. Ang-(1-7) also induces AKT phosphorylation. In addition, our analysis in vitro using C2C12 myotubes shows that Ang-(1-7), through a mechanism dependent on Mas, prevents the decrease in the levels of MHC and the increase in the expression of the atrogin-1 and MuRF-1, both induced by AngII. Ang-(1-7) ...
  http://www.clinsci.org/content/128/5/307
*  An Arabidopsis F-box protein acts as a transcriptional co-factor to regulate floral development | Development
Nearly 700 F-box proteins are predicted to be encoded in the Arabidopsis genome (Gagne et al., 2002; Kuroda et al., 2002). The striking expansion of this gene family probably reflects the recruitment of F-box proteins to regulate a variety of biological processes, implicating protein degradation as a prevalent developmental control mechanism in plants. For example, the Arabidopsis F-box protein TIR1 mediates hormonal signaling by acting as an auxin receptor; auxin binding to TIR1 promotes the activity of SCFTIR1 in targeting Aux/IAA proteins for destruction (Dharmasiri et al., 2005; Kepinski and Leyser, 2005; Tan et al., 2007). Here, we describe a new role for a plant F-box protein, in functioning as a component of the transcriptional machinery that regulates floral homeotic gene expression.. The work presented here provides evidence that ...
  http://dev.biologists.org/content/135/7/1235?ijkey=c85a6d71e07e52d197c4a57864c7103b14e523e8&keytype2=tf_ipsecsha
*  IJMS | Free Full-Text | The Role of F-Box Proteins during Viral Infection | HTML
The F-box domain is a protein structural motif of about 50 amino acids that mediates protein-protein interactions. The F-box protein is one of the four components of the SCF (SKp1, Cullin, F-box protein) complex, which mediates ubiquitination of proteins targeted for degradation by the proteasome, playing an essential role in many cellular processes. Several discoveries have been made on the use of the ubiquitin-proteasome system by viruses of several families to complete their infection cycle. On the other hand, F-box proteins can be used in the defense response by the host. This review describes the role of F-box proteins and the use of the ubiquitin-proteasome system in virus-host interactions.
  http://www.mdpi.com/1422-0067/14/2/4030/htm
*  Plus it
Resistance training is known to increase both anabolic and catabolic processes, presumably to ensure both quantitative as well as qualitative changes in the remodeling of skeletal muscle. In the present study we aimed to evaluate whether two training sessions separated by 48 h affected selected pathways in these two counteracting processes differently. The hypothesis of the present study was that a second exercise session following a 48-h recovery period should induce a larger effect on enzymes involved in protein synthesis and/or decrease the catabolic reactions evaluated as changes in mRNA expression of the muscle-specific ubiquitin ligases MAFbx and MuRF-1. The results indicate that repeated exercise sessions attenuate the increase in gene expression of MuRF-1 and further reduce the expression of MAFbx but have only a minor additional effect on enzymes regulating protein synthesis compared with a single session.. The resistance exercise led to increases ...
  http://ajpendo.physiology.org/content/294/1/E43
*  Plus it
Anthracyclines are widely used to treat cancers, but may also cause a cumulative dose-related cardiotoxicity. One possible mechanism of cardiotoxicity relates to effects on the cardiomyocyte proteasomal/ubiquitin system (UPS), the major protein recycling system in the cell. The goal of the research is to determine the impact of anthracyclines on the UPS and modulate cardiotoxicity by using other drugs in combination. Rats were treated chronically with a clinically relevant dose of daunorubicin (DNR) or the vehicle (controls) over 9 weeks. Another group received DNR pretreated with ICRF (dexrazoxane; a known protectant against anthracycline cardiotoxicity). The hearts were harvested one week later and analyzed. Isometric atrial preparations from rats treated with DNR showed decreased contractility compared to those pretreated with ICRF or with DNR vehicle only. Ubiquitin ligase atrogin-1 has been associated with muscle atrophy and cancer cachexia. In western blot analysis of common E3 ...
  http://cancerres.aacrjournals.org/content/72/8_Supplement/5675
*  ELM - instance DEG SCF TRCP1 1 in sequence P25963 at position 31
Dual phosphorylation of S32 and S36 in the TrCP1-binding motif of NF-kappa-B inhibitor alpha (NFKBIA) targets the protein to the SCF ubiquitin ligase complex, which marks it for degradation ...
  http://elm.eu.org/instances/DEG_SCF_TRCP1_1/P25963/31
*  F-box only protein 30
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and it is upregulated in nasopharyngeal carcinoma. [provided by RefSeq, Jul 2008 ...
  https://pharos.nih.gov/idg/targets/Q8TB52
*  F-box only protein 7
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and it may play a role in regulation of hematopoiesis. Alternatively spliced transcript variants of this gene have been identified with the full-length natures of only some variants being ...
  https://pharos.nih.gov/idg/targets/Q9Y3I1
*  F-box/LRR-repeat protein 8
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class. It shares 78% sequence identity with the mouse protein. [provided by RefSeq, Jul 2008 ...
  https://pharos.nih.gov/idg/targets/Q96CD0
*  The Dryad's Diatribe: SeeMyScience: Why is the Muscle Gone? Altered pathways in Cancer Cachexia.
The UPP is basically a system that uses ubiquitin molecules to tag proteins that are to be broken down. Ubiquitin is activated by an E1 enzyme, moves to a carrier protein (E2), which then recognizes E3 protein ligases. Two E3s in particular, known as MuRF1 (Muscle RING-finger protein-1) and Atrogin1/MAFbx (Muscle Atrophy F-box), have been suggested as key to the loss of muscle in wasting. Together, the E2 and E3 enzymes attach a chain of ubiquitin enzymes to the target protein (in our case a muscle protein), which can then be unfolded and broken down by a unit called a 'proteasome'. The proteasome breaks the protein into its smaller building blocks, peptides, which then get broken up into even smaller bits, amino acids. This sudden surge of amino acids can be used to make proteins that further drive the UPP, creating a vicious cycle of ...
  http://andanin.blogspot.com.au/2012/12/seemyscience-why-is-muscle-gone-altered.html
*  FBXO4 Full-Length MS Protein Standard - Creative Proteomics
FBXO4 Full-Length MS Protein Standard (NP_036308), Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine, was produced in human 293 cells (HEK293) with fully chemically defined cell culture medium to obtain incorporation efficiency at Creative-Proteomics. This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no ...
  https://www.creative-proteomics.com/product/detail-cpfl308458_3590.htm
*  Human Molecular Genetic and Functional Studies Identify TRIM63, Encoding Muscle RING Finger Protein 1, as a Novel Gene for...
We provide human molecular genetic and in vitro and in vivo functional evidence to implicate TRIM63, encoding MuRF1, an E3 ubiquitin ligase, as a likely causal gene for human HCM. The p.A48V, p.I130M and p.Q247* variants were exclusive to the HCM study population (whites) and were not detected in over 500 control white individuals. The small size of the families was not permissive to genotype-phenotype cosegregation analysis, which is a limitation of the study. The nonsense (p.Q247*) and the p.A48V variants recurred in 2 families. The nonsense variant led to premature truncation and loss of approximately one-third of the protein. The p.A48V and p.I130M variants affected highly conserved amino acids and were predicted-in silico-to be probably damaging to protein structure and function. Functionally, the variants had loss-of-function effects on E3 ubiquitin ligase activity, as detected in specialized ubiquitin-tagged HeLa cells, virally transduced adult cardiac myocytes, and ...
  http://circres.ahajournals.org/content/111/7/907
*  Mitochondrial F-box protein elisa and antibody
Shop Mitochondrial F-box protein ELISA Kit, Recombinant Protein and Mitochondrial F-box protein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
  https://www.mybiosource.com/protein_family.php?root=mitochondrial-f-box-protein
*  Plus it
The present study provides evidence that mRNA expression of atrogin-1 and MuRF-1 is coordinately upregulated in gastrocnemius between 12 h and 3 days after injury. However, at a later time point these atrogenes are differentially regulated. That is, atrogin-1 mRNA content was still increased 5 days after burn, whereas MuRF-1 mRNA had returned to control values by this time point. Hence, although both atrogin-1 and MuRF-1 might mediate the increased muscle proteolysis seen early after burn, any continued acceleration of protein breakdown at later times appears independent of MuRF-1. When examined 48 h after burn, the upregulation of both atrogin-1 and MuRF-1 occurred in the gastrocnemius, a muscle with a predominance of fast-twitch type II fibers, but not the soleus, a muscle composed primarily of slow-twitch type I fibers. Similarly, we confirmed that burn increased polyUb gene expression in the gastrocnemius (8, 18) and extend these observations by demonstrating no such change in soleus. ...
  http://ajpregu.physiology.org/content/292/1/R328
*  TRIP Database - CAND1 / TRP channels
Enhances transcription from various types of promoters (By similarity). Regulatory protein that interferes with the assembly of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex and thereby down-regulates ubiquitination of target proteins. Prevents neddylation of CUL1 by physically blocking access to the neddylation site. Disrupts interactions between CUL1 and SKP1 and between CUL1 and F-box proteins ...
  http://trpchannel.org/proteins/show?id=CAND1
*  F-box protein 42 ELISA Kits | Biocompare.com
Compare F-box protein 42 ELISA Kits from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more.
  https://www.biocompare.com/pfu/110627/soids/2-321903/Assay_Kit/ELISA_F-box_protein_42
*  F-box protein 17 ELISA Kits | Biocompare.com
Compare F-box protein 17 ELISA Kits from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more.
  http://www.biocompare.com/pfu/110627/soids/2-320062/Assay_Kit/ELISA_F-box_protein_17
*  FBXL12 - F-box/LRR-repeat protein 12 - Homo sapiens (Human) - FBXL12 gene & protein
Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Mediates the polyubiquitination and proteasomal degradation of CAMK1 leading to disruption of cyclin D1/CDK4 complex assembly which results in G1 cell cycle arrest in lung epithelia.
  http://www.uniprot.org/uniprot/Q9NXK8
*  FBXO17 - F-box only protein 17 - Homo sapiens (Human) - FBXO17 gene & protein
Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Able to recognize and bind denatured glycoproteins, which are modified with complex-type oligosaccharides. Also recognizes sulfated glycans. Does not bind high-mannose glycoproteins.
  http://www.uniprot.org/uniprot/Q96EF6
*  Fbxo27 - F-box only protein 27 - Mus musculus (Mouse) - Fbxo27 gene & protein
Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Able to recognize and bind complex-type oligosaccharides.
  http://www.uniprot.org/uniprot/Q6DIA9
*  F-Box Protein 8 (FBXO8) ELISA Kits
Reactivity: Chicken, Cow, Dog and more. Compare 12 different FBXO8 ELISA Kits & buy the right one directly at antibodies-online.com!
  http://www.antibodies-online.com/abstract/FBox+Protein+8+
*  FBXO28 Gene - GeneCards | FBX28 Protein | FBX28 Antibody
Complete information for FBXO28 gene (Protein Coding), F-Box Protein 28, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
  http://www.genecards.org/cgi-bin/carddisp.pl?gene=FBXO28
*  FBXO9 Gene - GeneCards | FBX9 Protein | FBX9 Antibody
Complete information for FBXO9 gene (Protein Coding), F-Box Protein 9, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
  http://www.genecards.org/cgi-bin/carddisp.pl?gene=FBXO9
*  Cullin 5抗体|Abcam中国
Cullin 5兔多克隆抗体(ab34840)可与人样本反应并经WB, IP, ELISA实验严格验证,被4篇文献引用。所有产品均提供质保服务,中国75%以上现货。
  http://www.abcam.cn/cullin-5-antibody-ab34840.html
*  重组人Skp1蛋白(ab117211)|Abcam中国
购买我们的重组人Skp1蛋白。Ab117211为全长蛋白,在大肠杆菌中生产并经过HPLC, SDS-PAGE实验验证。Abcam提供免费的实验方案,操作技巧及专业的支持。
  http://www.abcam.cn/recombinant-human-skp1-protein-ab117211.html