Evasion of the Innate Immune Response: the Old World Alphavirus nsP2 Protein Induces Rapid Degradation of Rpb1, a Catalytic...
The capsid protein of the OW alphaviruses, such as Sindbis (SINV), Semliki Forest (SFV), and Chikungunya (CHIKV) viruses, does ... we concluded that relocalization of nuclear proteins during wt SINV infection is not required for virus replication per se but ... SINV infection does not induce Rpb1 degradation in mosquito cells. (A) C7/10 cells were infected with SINV/GFP or SINV/G/GFP. ... we analyzed changes in Rpb1 levels in mosquito C7/10 cells after infection with SINV/GFP or SINV/G/GFP viruses. The antibodies ...https://jvi.asm.org/content/86/13/7180?ijkey=601e9ac5f2b9dd95647a31d6ffb50f0c359eda3f&keytype2=tf_ipsecsha
March 2016 - Small-Molecule Inhibitors as Anticancer Cell and Antiangiogenic Agents
Prominent examples of Old World alphaviruses comprise well-studied model viruses such as Semliki Forest virus (SFV) and Sindbis ... Replication complex internalization was observed upon infection of cells with SFV-wt and SFV mutants with deletions Gefitinib ( ... virus (SINV) as well as human pathogens such as chikungunya virus (CHIKV). CHIKV is spread by tropical mosquitoes of the family ... INTRODUCTION Alphaviruses are positive-sense RNA viruses grouped into the family and differentiated into Old World and New ...http://www.careersfromscience.org/index.php/2016/03/
Protocols and Video Articles Authored by Ken Olson (Translated to Turkish)
... high levels of virus replication were still observed during the initial infection. However, virus production subsequently ... Furthermore, in mosquito cells overexpressed LACV NSs was unable to inhibit RNAi against Semliki Forest virus. In mammalian ... SINV) is a mosquito-borne virus in the genus Alphavirus, family Togaviridae. Like most alphaviruses, SINVs exhibit lytic ... We used these mosquitoes to reveal effects of RNAi impairment in the midgut on SINV replication, midgut infection and ...https://www.jove.com/author/Ken_Olson?language=Turkish
The development of a reverse genetics system for the study of chikungunya pathogenesis in the mouse model - LSHTM Research...
By contrast, studies with other alphaviruses, most notably Sindbis and Semliki Forest viruses (SINV and SFV), have provided ... the virus replication cycle and its interactions with susceptible hosts. This tool was used to investigate the hypothesis that ... Faculty of Infectious and Tropical Diseases , Dept of Immunology and Infection. Faculty of Infectious and Tropical Diseases. ... It was also shown that the type 1 IFN response was induced at an earlier stage in mice challenged with virus containing an ...http://researchonline.lshtm.ac.uk/2305377/
Sindbis virus usurps the cellular HuR protein to stabilize its transcripts and promote productive infections in mammalian and...
Ranki M, Ulmanen I, Kaariainen L. Semliki Forest virus-specific nonstructural protein is associated with ribosomes. FEBS Lett. ... in human cells SinV infection induces a dramatic translocation of the HuR protein from the nucleus to the cytoplasm where SinV ... The alphaviruses: gene expression, replication, and evolution. Microbiol Rev. 1994;58:491-562. [PMC free article] [PubMed] ... or Semliki Forest Virus (SFV) were incubated in a cell-free mRNA decay assay with mosquito cell cytoplasmic extract. As show in ...http://pubmedcentralcanada.ca/pmcc/articles/PMC2929003/?lang=en-ca
Alphavirus Transducing System: Tools for Visualizing Infection in Mosquito Vectors | Protocol
Some alphaviruses require BSL3 containment. Figure 1: Overview of ATS virus production in BHK-21 cells using p5'dsMRE/GFP SINV ... Lundstrom, K. Expression of mammalian membrane proteins in mammalian cells using Semliki Forest virus vectors. Methods Mol. ... Wang, H. Effects of inducing or inhibiting apoptosis on Sindbis virus replication in mosquito cells. J. Gen. Virol. 89, 2651- ... cells following infection with SINV 5'dsMRE16/GFP virus.. Figure 3: Overview of an ATS virus infection in mosquitoes leading to ...https://www.jove.com/video/2363/alphavirus-transducing-system-tools-for-visualizing-infection