Publications - Institute for Bioengineering of Catalonia (IBEC)
Keywords: 2A strain 2457T, Escherichia-Coli, Salmonella-Enterica, Protein, DNA, Expression, Binding, HHA, Shigella, Plasmid ... The hallmark of prioniopathies is the presence of an abnormal conformational isoform (PrPsc) of the natural cellular prion ... In particular, mice lacking specific domains of the PrPc protein have revealed the contribution of these domains to ... Cholesterol depletion significantly affected the reciprocal distribution pattern of LFA-1 and GPI-APs in the resting state, and ...http://www.ibecbarcelona.eu/for-researchers/publications/2009/
Kuru prions and sporadic Creutzfeldt-Jakob disease prions have equivalent transmission properties in transgenic and wild-type...
... propagate as either variant CJD-like or sporadic CJD-like prion strains in transgenic mice expressing human prion protein. EMBO ... to an abnormal isoform, designated PrPSc (1, 2). This transition appears to involve only conformational change rather than ... data indicating that vCJD is a highly distinct human prion strain. The pathogenesis of vCJD differs significantly from that of ... of inoculated mice becoming infected (15, 26) (SI Table 1). Affected vCJD-inoculated Tg152 mice propagate a novel prion strain ...http://www.pnas.org/content/105/10/3885.long
Frontiers | The Molecular Mechanism of Natural Killer Cells Function and Its Importance in Cancer Immunotherapy | Immunology
In mice, NKG2D is present in two different isoforms, short isoform (NKG2D-S) and a long isoform (NKG2D-L), which are generated ... of death receptor with its cognate ligand induces a conformational change in the receptor and recruitment of adaptor protein ... NK cells are identified as CD3−NK1.1+ cells in C57BL/6, FVB/N, and NZB strains of mice. BALB/c, CBA/J, AKR, C3H, DBA/1, DBA/2, ... The level of expression of NKG2D ligands varies significantly between tumor types and stages of tumor progression. Ataxia ...https://www.frontiersin.org/articles/10.3389/fimmu.2017.01124/full
Pleiotropy of Glycogen Synthase Kinase-3 Inhibition by CHIR99021 Promotes Self-Renewal of Embryonic Stem Cells from Refractory...
Further, elevated c-Myc protein levels did not contribute significantly to CH-mediated ES cell self-renewal. Instead, the role ... and BALB/c mouse strains through stabilization of β-catenin and c-Myc protein levels. Stabilized β-catenin promoted ES self- ... β-catenin and c-Myc have similar effects on ES cells derived from both B6 and BALB/c mice. Conclusions/Significance Our data ... Here, we dissect the mechanism of GSK-3 inhibition by CHIR99021 in mouse ES cells from refractory mouse strains. Methodology/ ...http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0035892
Cutting Edge: Brucella abortus Exploits a Cellular Prion Protein on Intestinal M Cells as an Invasive Receptor | The Journal of...
... abortus into PPs was significantly reduced in Prnp−/− mice (Fig. 4). These data are consistent with the observation by Fontes ... Antigenic characterization of an abnormal isoform of prion protein using a new diverse panel of monoclonal antibodies. Virology ... because phagosomes containing a virB mutant strain of B. abortus fuse with lysosomes to form conventional phagolysosomes (12). ... replicates by interacting with cellular PrPC and mediating its conformational change into the disease-causing PrPSc (19). ...http://www.jimmunol.org/content/189/4/1540
Кирпичников Михаил Петрович - ИБХ РАН
The protein produced by the plasmid-free strain was purified and characterized to be hGH. The results demonstrates that a ... differing from the native protein only by one additional N-terminal Met residue. rSLURP-1 significantly inhibited proliferation ... conformational rearrangements of the P76GTKMIFA83 loop fragment have a significant effect on conformational mobility of the ... The venom manifested a very low acute toxicity with LD50 of about 190 mg/kg body weight in mice at subcutaneous (s.c.) ...http://www.ibch.ru/structure/groups/bioengineering/737
H eart Zip14 protein levels were significantly induced by 2% FeO, but there were no change s in heart DMT1 protein levels (Fig ... s uch as mice fed a high iron diet or Hfe knockout mice (61) On the other hand, TfR2 protein was down regulated in ... The acidic pH of the endosomal lumen induces a conformational change in Tf that results in iron release. The free Fe 3+ ... A) Transcript abundances of Zip14 (either Zip14 or long isoform of rZip14 (L Zip14)) and DMT1 with (+) or without ( ) IRE were ...http://ufdc.ufl.edu/UFE0042392/00001
Cross-sequence transmission of sporadic Creutzfeldt-Jakob disease creates a new prion strain
sporadic CJD-like prion strains in transgenic mice. expressing human prion protein. The EMBO Journal Vol. 21 No. 23 pp. 6358 ... prion protein (PrP) gene and the type (type 1 or type 2) of abnormal isoform. of PrP (PrP(Sc)) are major determinants of the ... sCJD-VV2 prions generates a new prion strain with. altered conformational properties and disease. phenotypes as p-dCJD prions. ... with vCJD presentation is significantly younger and the course of illness is. longer, the type and timing of psychiatric ...http://www.vegsource.com/talk/madcow/messages/1001524.html
Frontiers | Retinoic acid-related orphan receptors α and γ: key regulators of lipid/glucose metabolism, inflammation, and...
The resistance to hepatosteatosis in RORα-deficient mice is related to the reduced expression of several genes regulating lipid ... The resistance to hepatosteatosis in RORα-deficient mice is related to the reduced expression of several genes regulating lipid ... is considerably diminished in RORα-deficient mice as indicated by the reduced infiltration of M1 macrophages and decreased ... is considerably diminished in RORα-deficient mice as indicated by the reduced infiltration of M1 macrophages and decreased ...https://www.frontiersin.org/articles/10.3389/fendo.2013.00001/full
Publications | Reich Research Group - UC Santa Barbara
... protein function is fully compatible with these nanometal-DNA constructs. Significantly extending the range of optical based ... switching from unmethylated to hemimethylated cognate DNA involves delectable protein conformational changes 20-30 angstrom ... While a 10-fold attenuation was observed in the absence of DAM, the darn-strain was still able to infect and persist in the ... The major DNA cytosine methyltransferase isoform in mouse erythroleukemia cells, Dnmt1, exhibits potent dead-end inhibition ...http://www.chem.ucsb.edu/reichgroup/publications
A clinical study of kuru patients with long incubation periods at the end of the epidemic in Papua New Guinea | Philosophical...
... propagate as either variant CJD-like or sporadic CJD-like prion strains in transgenic mice expressing human prion protein. EMBO ... According to the 'protein-only' hypothesis (Griffith 1967), an abnormal PrP isoform is the principal, and possibly the sole, ... His astasia, gait ataxia and upper limb incoordination had worsened, but otherwise his clinical signs had not significantly ... by a post-translational process that involves conformational change and aggregation. ...http://rstb.royalsocietypublishing.org/content/363/1510/3725
Canonical and alternative transcript expression of PAX6 and CXCR4 in pancreatic cancer
The protein size is predominantly 48 kDa [as determined previously (18)], which corresponds to the PAX6 variant protein PAX6(5a ... Singh S, Mishra R, Arango NA, Deng JM, Behringer RR and Saunders GF: Iris hypoplasia in mice that lack the alternatively ... 2B). While the expression of the reporter was significantly increased with the addition of both isoforms, it is not clear ... isoform-specific PAX6 mouse mutants have overlapping but different phenotypes (33,34), and this is in parallel with human ...https://spandidos-publications.com/ol/13/6/4027
Cellular prion protein (PrPC) protects neuronal cells from the effect of huntingtin aggregation | Journal of Cell Science
Colling, S. B., Collinge, J. and Jefferys, J. G. R. (1996). Hippocampal slices from prion protein null mice: disrupted Ca2+- ... Cellular isoform of the prion protein PrPC in human intestinal cell lines: Genetic polymorphism at codon 129, mRNA ... Cellular prion protein inhibits proapoptotic Bax conformational change in human neurons and in breast carcinoma MCF-7 cells. ... 6A). In contrast to neuronal cells, depletion of PrPC from both intestinal cell lines did not significantly affect either ...http://jcs.biologists.org/content/120/15/2663
Chris Garcia | Stanford Medicine Profiles
... even though the Ab1 is an antibody to a peptide and the Ab3 is an antibody to a globular protein. Significantly, amino acid ... Mice transgenic for the TCRs have been generated and characterized in other laboratories. These analyses indicate that the mice ... The second isoform is less well defined (root-mean-square deviation from the average structure for backbone atoms of 1.07 A) ... Large conformational changes in three of the TCR CDR loops are induced upon binding, providing a mechanism of structural ...https://med.stanford.edu/profiles/chris-garcia?tab=teaching
Publications - Institute for Bioengineering of Catalonia (IBEC)
Keywords: QCM, Human plasma fibronectin, Induced conformational-changes, Von-willebrand-factor, BSA, Protein adsortion, Polymer ... Tg20 strain). By correlating histopathology with electrophysiology in living behaving mice, we demonstrate that both Prnp 2/2 ... The hallmark of prioniopathies is the presence of an abnormal conformational isoform (PrPsc) of the natural cellular prion ... Computed binding energies per peptide from -11.2 to -13.5 kcal/mol were calculated for GAGs and PVS, whereas a significantly ...http://www.ibecbarcelona.eu/for-researchers/publications/TOR/
Non-cell-autonomous retinoid signaling is crucial for renal development | Development
Mouse strains and genotyping. All matings were with Swiss-Webster mice (Taconic). Embryonic day 0.5 (E0.5) was considered to be ... 1999). Expression of green fluorescent protein in the ureteric bud of transgenic mice: a new tool for the analysis of ureteric ... 1990). Identification of a novel isoform of the retinoic acid receptor gamma expressed in the mouse embryo. Mol. Cell. Biol. 10 ... S1 in the supplementary material), suggesting that ureteric bud cultures were not significantly contaminated with mesenchyme. ...http://dev.biologists.org/content/137/2/283
RAGE (Receptor for Advanced Glycation Endproducts), RAGE Ligands, and their role in Cancer and Inflammation | Journal of...
It is found either as a membrane-bound or soluble protein that is markedly upregulated by stress in epithelial cells, thereby ... The lack of chromosomal protein Hmg1 does not disrupt cell growth but causes lethal hypoglycaemia in newborn mice. Nature ... although variants that would give rise to a soluble isoform are much rarer in mice . ... S100A6 binds to the tetramerization domain of p53 . S100A6 bound significantly to the C2 domain of RAGE, as opposed to the ...https://translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-7-17
The autoantibody levels in the control mice are not significantly different from the immunized mice (Fig. 1B). Although the ... 2000) Neurofibrillary tangles, amyotrophy and progressive motor disturbance in mice expressing mutant (P301L) tau protein. Nat ... 2000) Conformational change as one of the earliest alterations of tau in Alzheimer's disease. Neurobiol Aging 21:719-727. ... and contains all the microtubule binding repeats in the longest human tau isoform, was generously provided by Dr. M. Hutton ( ...http://www.jneurosci.org/content/27/34/9115
CHOROBA CREUTZFELDTA - JAKOBA
We found both high prion titers and the disease-causing isoform of the prion protein (PrPSc) in the skeletal muscle of wild- ... type mice inoculated with either the Me7 or Rocky Mountain Laboratory strain of murine prions. Particular muscles accumulated ... Operations associated with significantly increased risk included cardiac surgery, hysterectomy, haemorrhoidectomy, cataract ... Potential explanations focus on spontaneous somatic mutations in neuronal PRNP or rare stochastic conformational changes in ...http://www.halat.pl/cjd.html
1996 - Plateforme REgionale de CYtom trie pour la Microbiologie
Direct evidence of this was only provided for GAS strains which expressed a single M protein. The majority of GAS express ... Bacteriological cultures of livers, spleens and mesenteric lymph nodes from haemorrhaged mice given cytokine had significantly ... CD44s (standard isoform) levels and hyaluronan-binding activity were investigated in Balb/c 3T3 cells and their derivatives ... Almost all mutations in the amino-terminal one-third caused a conformational change of the envelope and a significant drop in ...https://precym.mio.univ-amu.fr/spip.php?article117
... and Akt3 protein expressed in all cell lines by immunoblotting using antibodes specific for each isoform (Fig. 2A). Also shown ... WT RhoC has significantly (P , 0.001) higher levels of 32P incorporation compared with the RhoCS73A mutant. These levels were ... A definitive role of RhoC in metastasis of orthotopic lung cancer in mice. Clin Cancer Res 2004;10:1192-200. ... Cell lines were authenticated by the Johns Hopkins Genomic Resource Center by STR analysis and maintained under defined culture ...http://mcr.aacrjournals.org/content/10/10/1306
Frontiers | Tissue-Specific Effects of Loss of Estrogen during Menopause and Aging | Endocrinology
Both strains of mice with osteoclast specific deletions of ERα have trabecular bone loss, highlighting the importance of ERα in ... activator protein-1, and cAMP response element binding protein in rat white adipocytes. Endocrinology 143, 930-940. ... The rs2234693 single nucleotide polymorphism (SNP) is associated with significantly increased risk of myocardial infarction (MI ... whereas the other isoform HSD11B2 catalyzes the back reaction. The enzyme HSD11B1 may play a role in adipose tissue development ...https://www.frontiersin.org/articles/10.3389/fendo.2012.00019/full
2009) Role of ADAMs in the ectodomain shedding and conformational conversion of the prion protein. J Biol Chem 284:22590-22600. ... TSEs result from the conversion of the cellular isoform of the prion protein (PrPC) into its β-sheet-rich pathological ... 1996) A vector for expressing foreign genes in the brains and hearts of transgenic mice. Genet Anal 13:159-163. ... Similarly, conditioned medium from Fv-PrP cells treated with AP20187 significantly reduced the number of cells positive for ...http://www.jneurosci.org/content/32/38/13255
Protocols and Video Articles Authored by David S. Wishart
The conformational conversion of the cellular prion protein (PrP(C)) to the β-rich infectious isoform PrP(Sc) is considered a ... knockout mice, would be significantly different from control C57BL/6J mice. Further, we hypothesised that any differences in ... To test our hypotheses colon tissues were collected from 16 male mice (FVB/N strain) and mounted in an Ussing chamber. ... and the mediation of protein-protein, protein-DNA, protein-RNA, and protein-cofactor interactions. NMR spectroscopy has long ...https://www.jove.com/author/David+S._Wishart
The origin of the prion agent of kuru: molecular and biological strain typing | Philosophical Transactions of the Royal Society...
1995 Unaltered susceptibility to BSE in transgenic mice expressing human prion protein. Nature. 378, 779-783. doi:10.1038/ ... 1996 Evidence for the conformation of the pathologic isoform of the prion protein enciphering and propagating prion diversity. ... These data are consistent with a conformational selection model of prion transmission barriers (Collinge 1999, 2001; Hill & ... these observations suggest that cerebellar onset and subsequent neuropathological changes in kuru may be significantly ...http://rstb.royalsocietypublishing.org/content/363/1510/3747