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*  ORBi: Browsing ORBi
Total proteins mixture will also be submitted to ESI-MS after HPLC separation as a means of improving both protein and proteome ... This will help to identify and validate biological targets in complex intracellular pathways to cure multifactorial diseases. ... Proteins were extracted from minutes amount of frozen hippocampal samples and subjected on two-dimensional difference in-gel ... Total proteins mixture will also be submitted to ESI-MS after HPLC separation as a means of improving both protein and proteome ...
  http://orbi.ulg.ac.be/browse?type=author&value=Soreq%2C+Hermona
*  Quantitative detection of Pf HRP2 in saliva of malaria patients in the Philippines | Malaria Journal | Full Text
All negative-control samples tested negative for salivary Pf HRP2. On a binary-decision basis, the ELISA agreed with microscopy ... A substantive difference between this protocol and previous related studies was that saliva samples were stabilized with ... Matched samples of blood and saliva were collected between January and May, 2011 from eight patients at Palawan Baptist ... Complex sample preparation and handling are not amenable to a low-cost rapid test. However, it is expected that short (i.e., ...
  https://malariajournal.biomedcentral.com/articles/10.1186/1475-2875-11-175
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Electrophoresis is used to separate complex mixtures of proteins (e.g., from cells, subcellular fractions, column fractions, or ... Intracellular Signaling Peptides and Proteins, Membrane Proteins, Isotope Labeling, Proteomics, plants, Arabidopsis thaliana, ... Also loss of material during extraction will affect both control and treatment samples in the same way, and therefore the ratio ... The procedure comprises the isolation of thylakoid membranes, followed by their separation into multiprotein complexes by ...
  https://www.jove.com/visualize/abstract/21541023/calcium-uptake-proton-transport-acidocalcisomes-toxoplasma
*  Protocols and Video Articles Authored by Mark Tobin (Translated to Korean)
... alteration and corrosion layers which are heterogeneous microlayered materials made by complex mixtures of organic and ... The rapid mineralisation leads to excellent preservation of the samples and a small crystal size. The value of IR spectroscopy ... Nov, 2014 , Pubmed ID: 25255060 Previous work has identified crystallin proteins extracted from fish eye lenses as a cheap and ... The protocol enables unprecedented correlated and complementary information on the intracellular biochemistry of metabolic ...
  https://www.jove.com/author/Mark_Tobin?language=Korean
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Electrophoresis is used to separate complex mixtures of proteins (e.g., from cells, subcellular fractions, column fractions, or ... Bacterial intracellular pathogens can be conceived as molecular tools to dissect cellular signaling cascades due to their ... Capillary electrophoresis separation of protein composition of ?-irradiated food pathogens Listeria monocytogenes and ... immunoprecipitates), to investigate subunit compositions, and to verify homogeneity of protein samples. It can also serve to ...
  https://www.jove.com/visualize/abstract/22427846/capillary-electrophoresis-separation-protein-composition-irradiated
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Electrophoresis is used to separate complex mixtures of proteins (e.g., from cells, subcellular fractions, column fractions, or ... The results showed specific labeling of a large number of cell surface proteins with minimal labeling of intracellular proteins ... Following separation by electrophoretic methods, proteins in a gel can be detected by several staining methods. This unit ... electrophoresis is used extensively for detection of biomarkers and other targets in complex protein samples to study ...
  https://www.jove.com/visualize/abstract/25919452/proteomic-analysis-copper-binding-proteins-excess-copper-stressed
*  Plus it
Quantification of syntaxin 1 was performed using ImageJ version 1.43u after separation of samples on 15% SDS-PAGE gels, ... C to complete complex formation. 25 μl of reaction mixture (50 μg of total protein) was diluted with 225 μl of 1× Laemmli ... 2014) How could SNARE proteins open a fusion pore? Physiology 29:278-285, doi:10.1152/physiol.00026.2013, pmid:24985331. ... The intracellular pipette solution contained the following (in mm): 136 KCl, 18 HEPES, 15 creatine phosphate, 4 Na-ATP, 4.6 ...
  http://www.jneurosci.org/content/36/47/11865
*  Sample Preparation Techniques for the Untargeted LC-MS-Based Discovery of Peptides in Complex Biological Matrices
... targeting multiple epitopes on the same proteins. Moreover, a mixture of polyclonal antibodies to distinct proteins are ... Because of the high complexity of biological samples, often a single fractionation or separation step is insufficient. ... When studying the intracellular proteome (or peptidome), cells are washed several times with FCS-free cell culture medium and/ ... Off-gel separation has not only shown applicability for proteins but also for peptides. Hubner et al. [54] compared the ...
  https://www.hindawi.com/journals/bmri/2011/245291/
*  Protocols and Video Articles Authored by Samuel L. Drennan
... and present a global analysis approach for the deconvolution of heterogeneous mixtures formed by weakly interacting proteins. ... This complex is also seen in a higher-resolution dATP-inhibited RNR structure presented here, which employs a distinct crystal ... With increasing the doping level, the concentration of trivalent Ce cations was increased in YDC samples and such trivalent Ce ... These two functions are necessary for full expression of the biological activity of the protein in intracellular lipid ...
  https://www.jove.com/author/Samuel+L._Drennan
*  Origin of Circulating Free DNA in Sepsis: Analysis of the CLP Mouse Model
... a mixture of chromosomal DNA and intracellular contents is extruded to the extracellular space as a fibrous structure upon a ... All samples were plated on sheep blood agar plates and incubated overnight at 37°C under aerobic conditions. The numbers of ... The proteins were detected upon incubation with horseradish peroxidase-conjugated anti-goat or anti-rabbit IgG (1 : 10,000) for ... PicoGreen specifically binds dsDNA, and after excitation at 485 nm, the dsDNA/PicoGreen fluorescence complex can be detected at ...
  https://www.hindawi.com/journals/mi/2015/614518/
*  Protocols and Video Articles Authored by Shuming Nie
... proteins, and intact viruses can be detected and identified in complex mixtures without target amplification or probe/target ... and intact viruses can be detected and identified in complex mixtures without target amplification or probe/target separation. ... Proton-sponge Coated Quantum Dots for SiRNA Delivery and Intracellular Imaging Journal of the American Chemical Society. Jul, ... a diagnostic tool to identify CXCR4 receptor-positive tumor cells in culture and tumors in paraffin-embedded clinical samples. ...
  https://www.jove.com/author/Shuming_Nie
*  Proteomes of Host Cell Membranes Modified by Intracellular Activities of Salmonella enterica
... is that typically the separation of proteins or peptides prior to MS is insufficient to fully resolve complex protein mixtures ... Samples of 7 μl each were applied to the columns and separated by a 60-min linear gradient from 5% to 50% solvent B (80% ... 47 proteins), endosomal and lysosomal proteins (9), Golgi proteins (14), and vesicle-transport-related proteins (24) have been ... 5B). A considerable number of SMM proteins were implicated in transport (65 proteins), vesicle trafficking (15 proteins), endo ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC4288265/
*  The use of proteomic techniques to study the physiology and virulence of staphylococcus aureus
The use of complex mix ture analysis coupled with HPLC separation to in crease the number of proteins identified from overnight ... This technique uses protein samples cleaved by a site specific enzyme, such as trypsin, for the iden tification of proteins ... 62 appears to be homologous to intracellular protease s This finding is odd because cytoplasmic proteins are not expected to be ... 35 The Use of Complex Mixture Analysis Coupled with HPLC Separation to I ncrease the Number of Proteins Identified from ...
  http://digital.lib.usf.edu/SFS0028061/00001
*  Protocols and Video Articles Authored by Thomas O. Metz
The MPLEx Protocol for Multi-omic Analyses of Soil Samples' ... identify tryptic peptides from complex mixtures of proteins, as ... constructed and evaluated for the effectiveness and reproducibility of the chromatographic separations using the above samples ... Intracellular fumarate concentration and succination of proteins are increased by hyperpolarization of the inner mitochondrial ... To demonstrate the performance of the method in the analysis of complex samples, we analyzed lipids extracted from a human cell ...
  https://www.jove.com/author/Thomas+O._Metz
*  Tumor-Associated a2 Vacuolar ATPase Acts As a Key Mediator of Cancer-Related Inflammation by Inducing Pro-Tumorigenic...
Lysate/probe mixtures were loaded onto 96-well plates and incubated with Abs for specific NF-κB proteins. Positive wells were ... Fold change was calculated by dividing treated samples by media control samples for each time point (n = 3). Protein secretion ... a2NTD-induced IL-1β secretion required an activated inflammasome, a protein complex used in the processing of pro-IL-1β into ... Intracellular ROS measurements. Two dyes were used to detect ROS: 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein ...
  http://www.jimmunol.org/content/186/3/1781.long
*  High-throughput identification of G protein-coupled receptor modulators through affinity mass spectrometry screening -...
... invalidated could have resulted from compound misidentification or altered binding properties in the original complex mixtures ... Chromatographic separation was performed on a ZORBAX Eclipse Plus C18 column (3.5 μm, 2.1 × 100 mm, Agilent) at a flow rate of ... All three proteins were expressed using the Bac-to-Bac Baculovirus Expression System (Invitrogen) in Spodoptera frugiperda (Sf9 ... The eluted samples were evaporated by a speed vacuum and reconstituted in 50% methanol before LC-MS analysis. The control ...
  http://pubs.rsc.org/en/content/articlehtml/2018/sc/c7sc04698g
*  Role of KaiC phosphorylation in the circadian clock system of Synechococcus elongatus PCC 7942 | PNAS
4C ), KaiC phosphorylation is required for the in vivo formation of the Kai proteins-SasA complex, as KaiA failed to interact ... intracellular accumulation of these proteins was assayed in the kaiA-flag strains (WT:KaiA-flag and S431A;T432A:KaiA-flag ... Prepared samples (incubation time 0) appeared as phosphorylated and nonphosphorylated bands (Fig. 2). In the absence of KaiA, ... In addition, the peptide (amino acids 427-434) was found to be a mixture of nonphosphorylated, singly phosphorylated, and ...
  http://www.pnas.org/content/101/38/13927
*  Immunofluorescence Protocol | Immunohistochemistry Protocol | Immunofluorescence Techniques| immunofluorescence staining...
Different fluorochromes with distinctexcitation and emission spectra can be used for the analysis of complex mixtures of ... Bauer KD and Jacobberger JW (1994) Analysis of intracellular proteins. Methods in Cell Biology 41: 351-376. ... In clinical practice, a large number of diagnostic procedures also depend on histological analysis of tissue samples, although ... Wright AP, Fitzgerald JJ and Colello RJ (1997) Rapid purification of glial cells using immunomagnetic separation. Journal of ...
  http://www.academicroom.com/topics/immunofluorescence-protocol
*  Patent US6949347 - Multiplex analysis using membrane-bound sensitizers - Google Patents
The released molecular tags are then identified by their distinct separation and detection characteristics. ... having phosphorylated intracellular portion (1103), receptor (1132) and intracellular proteins (1130) and (1136). After lysing ... Usually, such separation is accompanied by the differential movement of molecular tags from such a starting mixture along a ... Environmental samples include environmental material such as surface matter, soil, water and industrial samples, as well as ...
  http://www.google.com/patents/US6949347?dq=6437692
*  ChMob2 binds to ChCbk1 and promotes virulence and conidiation of the fungal pathogen Colletotrichum higginsianum | BMC...
The genome of C. higginsianum encodes two more Mob proteins. While we could not detect any effect on pathogenicity in ΔChmob3 ... Mob family proteins are conserved between animals, plants and fungi and are essential for the activation of NDR kinases that ... Histochemical samples or samples containing fluorescent reporter proteins were stained and analyzed by microscopy as described ... ChMob2 forms a complex with the conserved ChCbk1 Kinase. The study further analyzed the functions of the other two Mob proteins ...
  https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-017-0932-7
*  The Blog | Rise in clinical metabolomics will benefit from automated sample preparation
... and technicians with little to no experience or training in MS to be able to perform MS-based analyses of complex mixtures to ... Preparing samples for MS is not an easy task. Metabolomics differs from other types of omics, such as proteomics or lipidomics ... often combined with a prior separation step such as liquid or gas chromatography, makes it the preferred technique for ... since large and fast intracellular changes in metabolite concentrations are possible during biopsies." ...
  https://www.tecan.com/blog/rise-in-clinical-metabolomics-will-benefit-from-automated-sample-preparation
*  US Patent # 6,881,586. Retentate chromatography and protein chip arrays with applications in biology and medicine -...
... they can be used to isolate the target from complex mixtures, such as biological samples, and to detect the target by ... Frequently, a first separation of proteins by isoelectric focusing in a gel is coupled with a second separation by sodium ... In one embodiment, the ligand/receptor pair are a hormone and a cell surface receptor or an intracellular receptor. The ... Resolution does not require the complete separation of an analyte from all other analytes in a mixture. Rather, any separation ...
  http://patents.com/us-6881586.html
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
New work has shown that proteins involved in actin-based motility are also linked to autophagy, an intracellular degradation ... Characterization of Complex Systems Using the Design of Experiments Approach: Transient Protein Expression in Tobacco as a Case ... The genotyping protocol has an amplification rate of greater than 95% for plasma samples with a viral load ,1,000 HIV-1 RNA ... 1). The use of crude mixture circumvents the tedious process of purifying and identifying a suitable target from the microbe of ...
  https://www.jove.com/visualize/abstract/19214226/a-look-back-at-an-ongoing-problem-shigella-dysenteriae-type-1
*  ISCOMATRIX Adjuvant Combines Immune Activation with Antigen Delivery to Dendritic Cells In Vivo Leading to Effective Cross...
Samples were adjusted to 2 mg protein/ml as determined by Bradford assay (Bio-Rad). IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL- ... intracellular cytokine staining. ISCOM. Ag formulated with ISCOMATRIX adjuvant. LN. lymph node. VDLN. vaccine site-draining ... Untouched CD8α+ DCs were obtained by combining the Ab mixture of the mouse DC isolation kit (Invitrogen) with biotinylated anti ... This limitation can be circumvented by using full-length proteins, which 1) contain multiple MHC class I and MHC class II ...
  http://www.jimmunol.org/content/187/1/55
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
The described method does not require chromatographic separation of complex lipid mixtures recovered from yeast cells, thereby ... Intracellular Signaling Peptides and Proteins, Membrane Proteins, Isotope Labeling, Proteomics, plants, Arabidopsis thaliana, ... Moreover, OpNS can be a high‐throughput tool to examine hundreds of samples of small proteins. For example, the previously ... Membrane Proteins, Micelles, Molecular Motor Proteins, life sciences, biochemistry, Amino Acids, Peptides, and Proteins, lipid- ...
  https://www.jove.com/visualize/abstract/25221913/acidophilic-green-alga-pseudochlorella-sp-ykt1-accumulates-high