Putting an 'End' to HIV mRNAs: capping and polyadenylation as potential therapeutic targets | AIDS Research and Therapy | Full...
... communication between the stability of an RNA in the cytoplasm and its transcription rate [6, 7]. The processes of translation ... Berkhout B: HIV-1 as RNA evolution machine. RNA Biol. 2011, 8: 225-229. 10.4161/rna.8.2.14801View ArticlePubMedGoogle Scholar. ... RNA. 2013, 19: 443-460. 10.1261/rna.038596.113PubMed CentralView ArticlePubMedGoogle Scholar. ... RNA. 2004, 10: 1119-1129. 10.1261/rna.5263904PubMed CentralView ArticlePubMedGoogle Scholar. ...https://aidsrestherapy.biomedcentral.com/articles/10.1186/1742-6405-10-31
"THE ROLE OF ALTERNATIVE POLYADENYLATION MEDIATED BY CPSF30 IN <em>ARAB" by...
I tested this by assessing the influence poly(A) sites can have on the mRNA isoform's stability in the wild type and oxt6 ... Messenger RNA polyadenylation occurs in the nucleus, and the subunits of the polyadenylation complex that meditate this process ... I hypothesize that the stability of these various mRNA isoforms should be different, and that this is a possible way that ... My results show that most mRNA isoforms show similar stability profiles in the wild-type and mutant plants. However, the mRNA ...https://uknowledge.uky.edu/pss_etds/90/
RNA stability and storage
... Dr. Peter Gegenheimer pgegen at ku.nolospamare.edu Sat Jul 7 17:32:02 EST 2001 *Previous message: ... Record the final concentration of RNA in the ppt. When you want to use some of the RNA, mix the EtOH solution to restore the ... Measure the concentration of RNA (by any means you like). Ppt the RNA from 0.2 M NaOAc (pH 5.5) and 2.0 - 2.5 vol absolute EtOH ... Obviously freeze-thawing is not a worry in long-term storage, , but if you are using your RNA extracts every day and , storing ...http://www.bio.net/bionet/mm/methods/2001-July/089529.html
additional question: RNA stability and storage
... Dr. James J. Campanella campanellj at mail.montclair.edu Fri Jul 6 12:03:33 EST ... Obviously freeze-thawing is not a worry in long-term storage, ,but if you are using your RNA extracts every day and ,storing ... So how what percentage of loss does everyone think you will get per freeze-thaw cycle if the RNA is left in a single tube and ... them frozen, how do you ensure that you do not ,lose much RNA to degradation? Sorry if the question is a bit ,naive, but I have ...http://www.bio.net/bionet/mm/methods/2001-July/089518.html
Frontiers | ACTH Action on Messenger RNA Stability Mechanisms | Endocrinology
Modulation of messenger RNA stability is usually mediated by stabilizing or destabilizing RNA-binding proteins (RNA-BP) that ... Modulation of messenger RNA stability is usually mediated by stabilizing or destabilizing RNA-binding proteins (RNA-BP) that ... Failure of such mechanisms, in particular misexpression of RNA-BP, has been linked to several human diseases. In the adrenal ... Failure of such mechanisms, in particular misexpression of RNA-BP, has been linked to several human diseases. In the adrenal ...https://www.frontiersin.org/articles/10.3389/fendo.2017.00003/full
Regulated RNA stability in the Gram positives
Inhibition of translation in many cases has been shown to lead to a secondary effect on RNA stability, either because the RNA ... SR1 sRNA: curious effect of a small, regulatory RNA on mRNA stability. In the last decade, many small, regulatory RNAs (sRNAs) ... and there is no physical separation between the sites of RNA synthesis and RNA function, it is likely that RNA decay is a major ... Regulated RNA stability in the Gram positives. Ciarán Condon1 and David H. Bechhofer2,* ...http://pubmedcentralcanada.ca/pmcc/articles/PMC3078962/?lang=en-ca
Genome-wide analysis of long noncoding RNA stability. - Department of Psychiatry
Utilizing a custom noncoding RNA array, we determined the half-lives of ∼800 lncRNAs and ∼12,000 mRNAs in the mouse Neuro-2a ... Surprisingly, one of the least stable lncRNAs is the well-characterized paraspeckle RNA Neat1, suggesting Neat1 instability ... as well as lncRNAs showing extreme stability (half-life , 16 h). Analysis of lncRNA features revealed that intergenic and cis- ... antisense RNAs are more stable than those derived from introns, as are spliced lncRNAs compared to unspliced (single exon) ...https://www.psych.ox.ac.uk/publications/482396
The multifunctional protein glyceraldehyde-3-phosphate dehydrogenase is both regulated and controls colony-stimulating factor-1...
CSF-1 mRNA half-lives were decreased by 50% in the presence of GAPDH small interfering RNA. RNA footprinting analysis of the ... and we now recognize that one of the many functions of GAPDH is to promote mRNA stability of CSF-1, an important cytokine in ... We investigated the functional significance of GAPDH's association with CSF-1 mRNA and found that GAPDH small interfering RNA ... now adds regulation of mRNA stability to its repertoire. We are the first to evaluate the clinical role of GAPDH protein in ...https://www.semanticscholar.org/paper/The-multifunctional-protein-dehydrogenase-is-both-Zhou-Yi/b88fa924bb0fce27151a46249b1335e338894f4f
Multiple pathways are involved in the anoxia response of SKIP3 including HuR-regulated RNA stability, NF-kappaB and ATF4. - MRC...
... and RNA stabilization by HuR. Temporal activation of the NF-kappaB pathway under anoxia protected cells from negative ... RNA Stability, RNA, Messenger, RNA, Neoplasm, RNA-Binding Proteins, Repressor Proteins, Signal Transduction, Time Factors ... Multiple pathways are involved in the anoxia response of SKIP3 including HuR-regulated RNA stability, NF-kappaB and ATF4. ... Multiple pathways are involved in the anoxia response of SKIP3 including HuR-regulated RNA stability, NF-kappaB and ATF4. ...https://www.imm.ox.ac.uk/publications/125223
Time course analysis of RNA stability in human placenta | BMC Molecular Biology | Full Text
We tested and compared RNA yields, total RNA integrity, mRNA integrity and stability in these two protocols according to the ... Particularly, qRT-PCR data can be affected by RNA integrity and stability. To explore systematically how RNA quality affects ... C up to 48 h before RNA extraction without altering RNA quality. Rapid tissue handling without dissection and using RNA- ... stabilizing solution (RNAlater™) is a prerequisite to obtain suitable RNA integrity and stability. ...https://bmcmolbiol.biomedcentral.com/articles/10.1186/1471-2199-10-21
Study reviews stability of RNA within Ebola virus | Vaccine News
... including a look at the stability of RNA within the Ebola virus using urine samples and EDTA blood samples taken from people ... RNA showed stability for a minimum of 18 days, at which time the initial cycle threshold values amounted to ,30. RNA quickly ... Study reviews stability of RNA within Ebola virus Published on Tuesday, Nov 24, 2015 @ 10:14am by Vaccine News Reports ... From the few studies that have been conducted, it appears that RNA stability varies from five to 14 days when it comes to virus ...https://vaccinenewsdaily.com/stories/510649327-local-government-study-reviews-stability-of-rna-within-ebola-virus
The double life of UPF1 in RNA and DNA stability pathways - Infoscience
Intriguingly, the human NMD-kinase SMG1 is also involved in genome stability pathways and the human NMD-factor EST1A/SMG6 is ... The DNA and RNA helicase UPF1 is well known for its central role in Nonsense Mediated RNA Decay (NMD), which promotes ... of UPF1 and other NMD-factors in DNA replication and genome maintenance pathways and suggest functional connections between RNA ... The double life of UPF1 in RNA and DNA stability pathways Azzalin, C. M. ; Lingner, J. ...https://infoscience.epfl.ch/record/114205
Chemists Discover How Cells Create Stability During Critical DNA-to-RNA Information Transfers
RNA polymerase, RNA (red in illustration) and the DNA template ... ... Chemists Discover How Cells Create Stability During Critical DNA-to-RNA Information Transfers. December 29, 2009 in Chemistry ... In it, a flexible thread of RNA winds around the ladder-like DNA strand, creating a loop that locks them together as RNA ... Elongation involves RNA polymerase, an enzyme that makes RNA by first positioning itself next to DNA genes, then the DNA ...https://phys.org/print181325837.html
Evaluating the Stability of RNA-Seq Transcriptome Profiles in Whole Blood Over Time | RNA-Seq Blog
Thus, the stability of RNA-seq whole blood transcriptome can be verified by assessing ribosomal protein subunits and immune- ... Bowyer JF, Tranter KM, Hanig JP, Crabtree NM, Schleimer RP, George NI (2015) Evaluating the Stability of RNA-Seq Transcriptome ... stability of the control transcriptome over 2 years; and stability of the effects of AMPH on immune-related genes over 2 years ... Such stability enables the pooling of samples from replicate experiments to carry out differential expression analysis with ...https://www.rna-seqblog.com/evaluating-the-stability-of-rna-seq-transcriptome-profiles-in-whole-blood-over-time/
A long noncoding RNA promotes cellulase expression in Trichoderma reesei | Springer for Research & Development
Provided that the RNA length is a relevant property, we investigated if it might be determined by a difference in RNA stability ... RNA stability assay. The strains QM6a, QM9414, and Rut-C30 were cultivated in 25 ml MA medium containing 1% (w/v) α-d-lactose ... Additional file 5. RNA stability of the three HAX1 versions. The T. reesei strains QM6a (blue), QM9414 (yellow) and Rut-C30 ( ... RNA maps reveal new RNA classes and a possible function for pervasive transcription. Science. 2007;316:1484-8.CrossRefGoogle ...https://rd.springer.com/article/10.1186/s13068-018-1081-4
Dynamics and Stability of Individual Base Pairs in Two Homologous RNA-DNA Hybrids
Relative Thermodynamic Stability of DNA, RNA, and DNA:RNA Hybrid Duplexes: Relationship with base Composition and Structure. ... Thermodynamic and Structural Properties of Pentamer DNA·DNA, RNA·RNA, and DNA·RNA Duplexes of Identical Sequence. Biochemistry ... Dynamics and Stability of Individual Base Pairs in Two Homologous RNA-DNA Hybrids†. Yuegao Huang, Congju Chen,§ and Irina M. ... Stability and Properties of Double and Triple Helices: Dramatic Effects of RNA or DNA Backbone Composition. Science. 1992;258: ...http://pubmedcentralcanada.ca/pmcc/articles/PMC2758270/
Assessing and maximizing data quality in macromolecular crystallography. | Physician's Weekly
Abnormal RNA stability in amyotrophic lateral sclerosis.. August 15, 2018. Branched-chain amino acids in health and disease: ...https://www.physiciansweekly.com/assessing-and-maximizing-data-quality-in-macromolecular-crystallography/
Probing stability, specificity, and modular structure in group I intron RNAs
RNA structure Structural specificity RNA folding Bangia RNA Tetrahymena Introns Group I introns ... To explore further how RNAs gain stability from intricate architectures, we examined a novel group I intron from red algae ( ... Many functional RNAs are required to fold into specific three-dimensional structures. A fundamental property of RNA is that its ... This result underscores the modular nature of RNA motifs and provides insight into how structured RNAs can arrange helices and ...https://repositories.lib.utexas.edu/handle/2152/ETD-UT-2010-12-2198
Post‐transcriptional gene expression control by NANOS is up‐regulated and functionally important in pRb‐deficient cells | The...
RNA stability assays. Y79 retinoblastoma cells were depleted using shRNA specific to PUM1, PUM2, and scrambled controls and ... Double‐stranded RNA for RNAi experiments was generated using the RiboMax large scale RNA production system (Promega) following ... we conducted RNA‐stability assays on PUM substrates. To do this, we treated Y79 retinoblastoma cells depleted of PUM by shRNA ... Kedde M, van Kouwenhove M, Zwart W, Oude Vrielink JA, Elkon R, Agami R (2010) A Pumilio‐induced RNA structure switch in p27‐3′ ...http://emboj.embopress.org/content/early/2014/08/06/embj.201488057
CASP9 | Cancer Genetics Web
RNA Stability. *Survival Rate. *Tyrosine Transaminase. *Transferases (Other Substituted Phosphate Groups). *Apoptosis ... Recent studies have demonstrated that asymmetric small interfering RNA (asiRNA)-mediated gene silencing is more effective and ... Down-regulation of FoxM1 by thiostrepton or small interfering RNA inhibits proliferation, transformation ability and ... levels of LRPPRC were significantly decreased in the two PCa cell lines after transfection with small interfering RNA (siRNA)- ...http://www.cancerindex.org/geneweb/CASP9.htm
Giant viruses open Pandora's box | Bioinformati...
Movement of RNAs between cells of a single plant is well documented, but cross-species RNA transfer is largely unexplored. ... This makes perfect sense: "We hypothesized that disulfide bonds, by increasing protein stability, should make proteins' ... develop and apply MAGE-seq to identify RNA structures that determine optimal codon preferences in an essential E. coli gene. ... is a parasitic plant that forms symplastic connections with its hosts and takes up host messenger RNAs (mRNAs). We sequenced ...https://www.scoop.it/t/bioinformatics-comparative-genomics-and-molecular-evolution/p/4005731099/2013/08/06/giant-viruses-open-pandora-s-box
SciCombinator - Systematic discovery of structural elements governing stability of mammalian messenger RNAs.
Systematic discovery of structural elements governing stability of mammalian messenger RNAs.'. ... RNA Stability, RNA, Messenger, RNA, Small Interfering, Time Factors, Transcription, Genetic. ... RNA splicing, Intron, Secondary structure, Cis-regulatory element, Gene expression, DNA, Messenger RNA, RNA MeSH headings. 3' ... Systematic discovery of structural elements governing stability of mammalian messenger RNAs. OPEN Nature , 12 Apr 2012 ...http://scicombinator.com/articles/16666
Lactococcus lactis peptidoglycane
Previous message: synthetic RNA stability/degradation *Next message: BeadBeater vs. The Sonicator ...http://www.bio.net/bionet/mm/methods/1994-December/022102.html
Tissue-Specific Stem Cells & Therapy | Yale Stem Cell Center | Yale School of Medicine
Cardiology; Endothelium; Immune System; Inflammation; Macrophages; RNA Stability; T-Lymphocytes Clinical Interests. ...https://medicine.yale.edu/stemcell/people/research/therapy.aspx
Xpert search results for Heuristic local search tutorial
Understand RNA splicing and explain its role in regulating gene expression. Describe the importance of RNA stability in […]. ...https://www.nottingham.ac.uk/xpert/scoreresults.php?keywords=Heuristic%20local%20search%20tutorial&start=1500&end=1520