*  Measurement of mRNA Decay in Mouse Embryonic Fibroblasts -BIO-PROTOCOL
mRNA stability control is a critical step in the post-transcriptional regulation of gene expression. Actinomycin D, an ... Take 1 μl of RNA from each sample to check for RNA quantity and quality with Nanodrop.. Note: The expected RNA yield from three ... Figure 2. Stability of Fos and Cxcl1 transcripts after TNF stimulation in the presence and absence of TTP. Actinomycin D, to ... RNA extraction, reverse transcription and semi-quantitative real-time PCR. * To prepare cells for RNA extraction, the culture ...
  http://www.bio-protocol.org/e1858
*  Ching-Yi Chen, PhD | Nutrition & Obesity Research Center
mRNA stability varies considerably from one mRNA species to another and is determined by specific cis-acting elements within ... Gherzi, R., Lee, K.-Y., Briata, P., Wegmüller, D., Moroni, C., Karin, M. and Chen, C.Y. (2004). A KH domain RNA binding protein ... The RNA-binding protein KSRP promotes decay of -catenin mRNA and is inactivated by PI3K-AKT signaling. PLos Biol. 5, 82-95. ... AMD is regulated by RNA-binding proteins. A number of proteins have been described to bind AREs and are collectively called ARE ...
  http://www.norc.uab.edu/people/cchen
*  RNA stability and storage
... Dr. Peter Gegenheimer pgegen at ku.nolospamare.edu Sat Jul 7 17:32:02 EST 2001 *Previous message: ... Record the final concentration of RNA in the ppt. When you want to use some of the RNA, mix the EtOH solution to restore the ... Measure the concentration of RNA (by any means you like). Ppt the RNA from 0.2 M NaOAc (pH 5.5) and 2.0 - 2.5 vol absolute EtOH ... Obviously freeze-thawing is not a worry in long-term storage, , but if you are using your RNA extracts every day and , storing ...
  http://www.bio.net/bionet/mm/methods/2001-July/089529.html
*  additional question: RNA stability and storage
... Dr. James J. Campanella campanellj at mail.montclair.edu Fri Jul 6 12:03:33 EST ... Obviously freeze-thawing is not a worry in long-term storage, ,but if you are using your RNA extracts every day and ,storing ... So how what percentage of loss does everyone think you will get per freeze-thaw cycle if the RNA is left in a single tube and ... them frozen, how do you ensure that you do not ,lose much RNA to degradation? Sorry if the question is a bit ,naive, but I have ...
  http://www.bio.net/bionet/mm/methods/2001-July/089518.html
*  Frontiers | ACTH Action on Messenger RNA Stability Mechanisms | Endocrinology
Modulation of messenger RNA stability is usually mediated by stabilizing or destabilizing RNA-binding proteins (RNA-BP) that ... Modulation of messenger RNA stability is usually mediated by stabilizing or destabilizing RNA-binding proteins (RNA-BP) that ... Failure of such mechanisms, in particular misexpression of RNA-BP, has been linked to several human diseases. In the adrenal ... Failure of such mechanisms, in particular misexpression of RNA-BP, has been linked to several human diseases. In the adrenal ...
  https://www.frontiersin.org/articles/10.3389/fendo.2017.00003/full
*  Regulated RNA stability in the Gram positives
Inhibition of translation in many cases has been shown to lead to a secondary effect on RNA stability, either because the RNA ... SR1 sRNA: curious effect of a small, regulatory RNA on mRNA stability. In the last decade, many small, regulatory RNAs (sRNAs) ... and there is no physical separation between the sites of RNA synthesis and RNA function, it is likely that RNA decay is a major ... Regulated RNA stability in the Gram positives. Ciarán Condon1 and David H. Bechhofer2,* ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC3078962/?lang=en-ca
*  Genome-wide analysis of long noncoding RNA stability. - Department of Psychiatry
Utilizing a custom noncoding RNA array, we determined the half-lives of ∼800 lncRNAs and ∼12,000 mRNAs in the mouse Neuro-2a ... Surprisingly, one of the least stable lncRNAs is the well-characterized paraspeckle RNA Neat1, suggesting Neat1 instability ... as well as lncRNAs showing extreme stability (half-life , 16 h). Analysis of lncRNA features revealed that intergenic and cis- ... antisense RNAs are more stable than those derived from introns, as are spliced lncRNAs compared to unspliced (single exon) ...
  https://www.psych.ox.ac.uk/publications/482396
*  Study reviews stability of RNA within Ebola virus | Vaccine News
... including a look at the stability of RNA within the Ebola virus using urine samples and EDTA blood samples taken from people ... RNA showed stability for a minimum of 18 days, at which time the initial cycle threshold values amounted to ,30. RNA quickly ... Study reviews stability of RNA within Ebola virus Published on Tuesday, Nov 24, 2015 @ 10:14am by Vaccine News Reports ... From the few studies that have been conducted, it appears that RNA stability varies from five to 14 days when it comes to virus ...
  https://vaccinenewsdaily.com/stories/510649327-local-government-study-reviews-stability-of-rna-within-ebola-virus
*  Chemists Discover How Cells Create Stability During Critical DNA-to-RNA Information Transfers
RNA polymerase, RNA (red in illustration) and the DNA template ... ... Chemists Discover How Cells Create Stability During Critical DNA-to-RNA Information Transfers. December 29, 2009 in Chemistry ... In it, a flexible thread of RNA winds around the ladder-like DNA strand, creating a loop that locks them together as RNA ... Elongation involves RNA polymerase, an enzyme that makes RNA by first positioning itself next to DNA genes, then the DNA ...
  https://phys.org/print181325837.html
*  Dynamics and Stability of Individual Base Pairs in Two Homologous RNA-DNA Hybrids
Relative Thermodynamic Stability of DNA, RNA, and DNA:RNA Hybrid Duplexes: Relationship with base Composition and Structure. ... Thermodynamic and Structural Properties of Pentamer DNA·DNA, RNA·RNA, and DNA·RNA Duplexes of Identical Sequence. Biochemistry ... Dynamics and Stability of Individual Base Pairs in Two Homologous RNA-DNA Hybrids†. Yuegao Huang, Congju Chen,§ and Irina M. ... Stability and Properties of Double and Triple Helices: Dramatic Effects of RNA or DNA Backbone Composition. Science. 1992;258: ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC2758270/
*  Probing stability, specificity, and modular structure in group I intron RNAs
RNA structure Structural specificity RNA folding Bangia RNA Tetrahymena Introns Group I introns ... To explore further how RNAs gain stability from intricate architectures, we examined a novel group I intron from red algae ( ... Many functional RNAs are required to fold into specific three-dimensional structures. A fundamental property of RNA is that its ... This result underscores the modular nature of RNA motifs and provides insight into how structured RNAs can arrange helices and ...
  https://repositories.lib.utexas.edu/handle/2152/ETD-UT-2010-12-2198
*  Post‐transcriptional gene expression control by NANOS is up‐regulated and functionally important in pRb‐deficient cells | The...
RNA stability assays. Y79 retinoblastoma cells were depleted using shRNA specific to PUM1, PUM2, and scrambled controls and ... Double‐stranded RNA for RNAi experiments was generated using the RiboMax large scale RNA production system (Promega) following ... we conducted RNAstability assays on PUM substrates. To do this, we treated Y79 retinoblastoma cells depleted of PUM by shRNA ... Kedde M, van Kouwenhove M, Zwart W, Oude Vrielink JA, Elkon R, Agami R (2010) A Pumilio‐induced RNA structure switch in p27‐3′ ...
  http://emboj.embopress.org/content/early/2014/08/06/embj.201488057
*  CASP9 | Cancer Genetics Web
RNA Stability. *Survival Rate. *Tyrosine Transaminase. *Transferases (Other Substituted Phosphate Groups). *Apoptosis ... Recent studies have demonstrated that asymmetric small interfering RNA (asiRNA)-mediated gene silencing is more effective and ... Down-regulation of FoxM1 by thiostrepton or small interfering RNA inhibits proliferation, transformation ability and ... levels of LRPPRC were significantly decreased in the two PCa cell lines after transfection with small interfering RNA (siRNA)- ...
  http://www.cancerindex.org/geneweb/CASP9.htm
*  Giant viruses open Pandora's box | Bioinformati...
Movement of RNAs between cells of a single plant is well documented, but cross-species RNA transfer is largely unexplored. ... This makes perfect sense: "We hypothesized that disulfide bonds, by increasing protein stability, should make proteins' ... develop and apply MAGE-seq to identify RNA structures that determine optimal codon preferences in an essential E. coli gene. ... is a parasitic plant that forms symplastic connections with its hosts and takes up host messenger RNAs (mRNAs). We sequenced ...
  https://www.scoop.it/t/bioinformatics-comparative-genomics-and-molecular-evolution/p/4005731099/2013/08/06/giant-viruses-open-pandora-s-box
*  SciCombinator - Systematic discovery of structural elements governing stability of mammalian messenger RNAs.
Systematic discovery of structural elements governing stability of mammalian messenger RNAs.'. ... RNA Stability, RNA, Messenger, RNA, Small Interfering, Time Factors, Transcription, Genetic. ... RNA splicing, Intron, Secondary structure, Cis-regulatory element, Gene expression, DNA, Messenger RNA, RNA MeSH headings. 3' ... Systematic discovery of structural elements governing stability of mammalian messenger RNAs. OPEN Nature , 12 Apr 2012 ...
  http://scicombinator.com/articles/16666
*  Lactococcus lactis peptidoglycane
Previous message: synthetic RNA stability/degradation *Next message: BeadBeater vs. The Sonicator ...
  http://www.bio.net/bionet/mm/methods/1994-December/022102.html
*  Xpert search results for Heuristic local search tutorial
Understand RNA splicing and explain its role in regulating gene expression. Describe the importance of RNA stability in […]. ...
  https://www.nottingham.ac.uk/xpert/scoreresults.php?keywords=Heuristic%20local%20search%20tutorial&start=1500&end=1520
*  Patent US6677606 - Dopa and dopamine modification of metal oxide semiconductors, method for ... - Google Patents
In Splicing, RNA Processing, or RNA Stability. Nanocrystalline titania films on glass mesh or other supports modified with DNA ... Stability of the construct is larger than the stability of a complexes between TiO2 and glycidil, glycidil isopropyl ether, ... or RNA of defined sequence will selectively bind proteins that recognize the sequence of RNA and participate in splicing, RNA ... Selectivity (stringency) of hybridization can be adjusted to the thermal stability of each individual oligonucleotide and ...
  http://www.google.com/patents/US6677606?dq=inassignee:doubleclick
*  All Categories: Antibodies, ELISA Kits, Lysates | Cell Signaling
Regulatory RNA (siRNA, miRNA, sRNA) (13). RNA Splicing (4). RNA Stability (237). snRNP Regulation (2). Ser/Thr Kinases / ... Protein Folding / Stability (90). Protein Translation (140). RNA Processing (330). mRNA Capping (29). mRNA Decay (88). ...
  https://www.cellsignal.com/browse/rna-processing-rna-stability?N=4294960009+4294956287
*  Glucocorticoids Regulate V1a Vasopressin Receptor Expression by Increasing mRNA Stability in Vascular Smooth Muscle Cells |...
Insights into hormonal control of messenger RNA stability. Mol Endocrinol.. 1990;4:953-957. ... Transcript Stability Analysis. The stability of V1a receptor mRNA in dexamethasone-treated and control cells was measured by ... Effects of Glucocorticoids on V1a Receptor mRNA Stability and Gene Transcription. We examined V1a receptor mRNA stability by ... Blots and graph show effects of dexamethasone and cycloheximide on V1a receptor mRNA stability. V1a receptor mRNA stability was ...
  http://hyper.ahajournals.org/content/26/4/665
*  To whom working with DNA sequencing - Biology-Online
besides think about RNA stability.. 2- think about the termination mechanism and the relation between probability of synthesis ... 1. Would a primer extension reaction in DNA sequencing work using an RNA polymerase? any one can explain this point please? 2. ... http://en.wikipedia.org/wiki/Rna_Polymerase. Patrick Science has proof without any certainty. Creationists have certainty ...
  https://www.biology-online.org/biology-forum/viewtopic.php?t=13142
*  DCB - Cancer Cell Biology Research - National Cancer Institute
The role of non-coding RNAs and RNA binding proteins in the regulation of splicing, translation, and mRNA stability ... relevant to this research include the study of chemical modifications to RNA and RNA binding proteins that affect RNA stability ... Altered mechanisms affecting RNA stability, splicing, transport, and mRNA translation. *Regulation and mechanism of alternative ... Mechanisms that alter protein stability and function in cancer cells, including proteasome-mediated degradation and protein ...
  https://www.cancer.gov/about-nci/organization/dcb/research-portfolio/ccbr
*  Difference between revisions of "Sauer:RNA Purification from E. coli" - OpenWetWare
Notes on RNA stability. I have seen so many people freak out about having an "RNase-Free" environment. Washing benches, buying ... Four samples of RNA resolved so that the smallest and largest RNAs are visible. Middle: RNA resolved such that larger RNAs are ... 1 My Experience Purifying RNA from E. coli. *2 My Current RNA isolation protocol for E. coli *2.1 For unstable RNAs like mRNAs ... My Current RNA isolation protocol for E. coli. For unstable RNAs like mRNAs. Immediately stabilize the RNA, I like to add one ...
  https://openwetware.org/wiki/?title=Sauer:RNA_Purification_from_E._coli&diff=140967&oldid=95115
*  Common and distinct organ and stress responsive transcriptomic patterns in Oryza sativa and Arabidopsis thaliana
Circadian control of messenger RNA stability. Association with a sequence-specific messenger RNA decay pathway. Plant Physiol. ... The RNA was isolated using the Qiagen RNeasy Plant RNA isolation kit and DNase treated using both the Qiagen on-column DNase ... Isolated RNA was quantitated using a Nanodrop spectrophotometer and 1 μg of total RNA was reverse transcribed using the Bio-Rad ... Keller W. No end yet to messenger RNA 3' processing! Cell. 1995;81(6):829-832. doi: 10.1016/0092-8674(95)90001-2. [PubMed] [ ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC3095337/