*  Measurement of mRNA Decay in Mouse Embryonic Fibroblasts -BIO-PROTOCOL
mRNA stability control is a critical step in the post-transcriptional regulation of gene expression. Actinomycin D, an ... Take 1 μl of RNA from each sample to check for RNA quantity and quality with Nanodrop.. Note: The expected RNA yield from three ... Figure 2. Stability of Fos and Cxcl1 transcripts after TNF stimulation in the presence and absence of TTP. Actinomycin D, to ... RNA extraction, reverse transcription and semi-quantitative real-time PCR. * To prepare cells for RNA extraction, the culture ...
  http://www.bio-protocol.org/e1858
*  Genome-wide analysis of mRNA decay patterns during early Drosophiladevelopment | Genome Biology | Full Text
RNA localization and, in the case of yps, splicing [83-89], play an additional role in RNA stability control. ... miRNA, microRNA; piRNA, piwi-interacting RNA; RNAi, RNA interference; siRNA, small interfering RNA. ... RNA stability was positively correlated with active translation and rising protein levels, while RNA decay was associated with ... was performed to address the correlation between AREs and RNA stability. We found that RNA decay (classes II, IV and V) is ...
  https://genomebiology.biomedcentral.com/articles/10.1186/gb-2010-11-9-r93
*  Ching-Yi Chen, PhD | Nutrition & Obesity Research Center
mRNA stability varies considerably from one mRNA species to another and is determined by specific cis-acting elements within ... Gherzi, R., Lee, K.-Y., Briata, P., Wegmüller, D., Moroni, C., Karin, M. and Chen, C.Y. (2004). A KH domain RNA binding protein ... The RNA-binding protein KSRP promotes decay of -catenin mRNA and is inactivated by PI3K-AKT signaling. PLos Biol. 5, 82-95. ... AMD is regulated by RNA-binding proteins. A number of proteins have been described to bind AREs and are collectively called ARE ...
  http://www.norc.uab.edu/people/cchen
*  RNA stability and storage
... Dr. Peter Gegenheimer pgegen at ku.nolospamare.edu Sat Jul 7 17:32:02 EST 2001 *Previous message: ... Record the final concentration of RNA in the ppt. When you want to use some of the RNA, mix the EtOH solution to restore the ... Measure the concentration of RNA (by any means you like). Ppt the RNA from 0.2 M NaOAc (pH 5.5) and 2.0 - 2.5 vol absolute EtOH ... Obviously freeze-thawing is not a worry in long-term storage, , but if you are using your RNA extracts every day and , storing ...
  http://www.bio.net/bionet/mm/methods/2001-July/089529.html
*  additional question: RNA stability and storage
... Dr. James J. Campanella campanellj at mail.montclair.edu Fri Jul 6 12:03:33 EST ... Obviously freeze-thawing is not a worry in long-term storage, ,but if you are using your RNA extracts every day and ,storing ... So how what percentage of loss does everyone think you will get per freeze-thaw cycle if the RNA is left in a single tube and ... them frozen, how do you ensure that you do not ,lose much RNA to degradation? Sorry if the question is a bit ,naive, but I have ...
  http://www.bio.net/bionet/mm/methods/2001-July/089518.html
*  Frontiers | ACTH Action on Messenger RNA Stability Mechanisms | Endocrinology
Modulation of messenger RNA stability is usually mediated by stabilizing or destabilizing RNA-binding proteins (RNA-BP) that ... Modulation of messenger RNA stability is usually mediated by stabilizing or destabilizing RNA-binding proteins (RNA-BP) that ... Failure of such mechanisms, in particular misexpression of RNA-BP, has been linked to several human diseases. In the adrenal ... Failure of such mechanisms, in particular misexpression of RNA-BP, has been linked to several human diseases. In the adrenal ...
  https://www.frontiersin.org/articles/10.3389/fendo.2017.00003/full
*  Regulated RNA stability in the Gram positives
Inhibition of translation in many cases has been shown to lead to a secondary effect on RNA stability, either because the RNA ... SR1 sRNA: curious effect of a small, regulatory RNA on mRNA stability. In the last decade, many small, regulatory RNAs (sRNAs) ... and there is no physical separation between the sites of RNA synthesis and RNA function, it is likely that RNA decay is a major ... Regulated RNA stability in the Gram positives. Ciarán Condon1 and David H. Bechhofer2,* ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC3078962/?lang=en-ca
*  Genome-wide analysis of long noncoding RNA stability. - Department of Psychiatry
Utilizing a custom noncoding RNA array, we determined the half-lives of ∼800 lncRNAs and ∼12,000 mRNAs in the mouse Neuro-2a ... Surprisingly, one of the least stable lncRNAs is the well-characterized paraspeckle RNA Neat1, suggesting Neat1 instability ... as well as lncRNAs showing extreme stability (half-life , 16 h). Analysis of lncRNA features revealed that intergenic and cis- ... antisense RNAs are more stable than those derived from introns, as are spliced lncRNAs compared to unspliced (single exon) ...
  https://www.psych.ox.ac.uk/publications/482396
*  Study reviews stability of RNA within Ebola virus | Vaccine News
... including a look at the stability of RNA within the Ebola virus using urine samples and EDTA blood samples taken from people ... RNA showed stability for a minimum of 18 days, at which time the initial cycle threshold values amounted to ,30. RNA quickly ... Study reviews stability of RNA within Ebola virus Published on Tuesday, Nov 24, 2015 @ 10:14am by Vaccine News Reports ... From the few studies that have been conducted, it appears that RNA stability varies from five to 14 days when it comes to virus ...
  https://vaccinenewsdaily.com/stories/510649327-local-government-study-reviews-stability-of-rna-within-ebola-virus
*  Chemists Discover How Cells Create Stability During Critical DNA-to-RNA Information Transfers
RNA polymerase, RNA (red in illustration) and the DNA template ... ... Chemists Discover How Cells Create Stability During Critical DNA-to-RNA Information Transfers. December 29, 2009 in Chemistry ... In it, a flexible thread of RNA winds around the ladder-like DNA strand, creating a loop that locks them together as RNA ... Elongation involves RNA polymerase, an enzyme that makes RNA by first positioning itself next to DNA genes, then the DNA ...
  https://phys.org/print181325837.html
*  A long noncoding RNA promotes cellulase expression in Trichoderma reesei | Springer for Research & Development
Provided that the RNA length is a relevant property, we investigated if it might be determined by a difference in RNA stability ... RNA stability assay. The strains QM6a, QM9414, and Rut-C30 were cultivated in 25 ml MA medium containing 1% (w/v) α-d-lactose ... Additional file 5. RNA stability of the three HAX1 versions. The T. reesei strains QM6a (blue), QM9414 (yellow) and Rut-C30 ( ... RNA maps reveal new RNA classes and a possible function for pervasive transcription. Science. 2007;316:1484-8.CrossRefGoogle ...
  https://rd.springer.com/article/10.1186/s13068-018-1081-4
*  Dynamics and Stability of Individual Base Pairs in Two Homologous RNA-DNA Hybrids
Relative Thermodynamic Stability of DNA, RNA, and DNA:RNA Hybrid Duplexes: Relationship with base Composition and Structure. ... Thermodynamic and Structural Properties of Pentamer DNA·DNA, RNA·RNA, and DNA·RNA Duplexes of Identical Sequence. Biochemistry ... Dynamics and Stability of Individual Base Pairs in Two Homologous RNA-DNA Hybrids†. Yuegao Huang, Congju Chen,§ and Irina M. ... Stability and Properties of Double and Triple Helices: Dramatic Effects of RNA or DNA Backbone Composition. Science. 1992;258: ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC2758270/
*  Probing stability, specificity, and modular structure in group I intron RNAs
RNA structure Structural specificity RNA folding Bangia RNA Tetrahymena Introns Group I introns ... To explore further how RNAs gain stability from intricate architectures, we examined a novel group I intron from red algae ( ... Many functional RNAs are required to fold into specific three-dimensional structures. A fundamental property of RNA is that its ... This result underscores the modular nature of RNA motifs and provides insight into how structured RNAs can arrange helices and ...
  https://repositories.lib.utexas.edu/handle/2152/ETD-UT-2010-12-2198
*  Post‐transcriptional gene expression control by NANOS is up‐regulated and functionally important in pRb‐deficient cells | The...
RNA stability assays. Y79 retinoblastoma cells were depleted using shRNA specific to PUM1, PUM2, and scrambled controls and ... Double‐stranded RNA for RNAi experiments was generated using the RiboMax large scale RNA production system (Promega) following ... we conducted RNAstability assays on PUM substrates. To do this, we treated Y79 retinoblastoma cells depleted of PUM by shRNA ... Kedde M, van Kouwenhove M, Zwart W, Oude Vrielink JA, Elkon R, Agami R (2010) A Pumilio‐induced RNA structure switch in p27‐3′ ...
  http://emboj.embopress.org/content/early/2014/08/06/embj.201488057
*  CASP9 | Cancer Genetics Web
RNA Stability. *Survival Rate. *Tyrosine Transaminase. *Transferases (Other Substituted Phosphate Groups). *Apoptosis ... Recent studies have demonstrated that asymmetric small interfering RNA (asiRNA)-mediated gene silencing is more effective and ... Down-regulation of FoxM1 by thiostrepton or small interfering RNA inhibits proliferation, transformation ability and ... levels of LRPPRC were significantly decreased in the two PCa cell lines after transfection with small interfering RNA (siRNA)- ...
  http://www.cancerindex.org/geneweb/CASP9.htm
*  Giant viruses open Pandora's box | Bioinformati...
Movement of RNAs between cells of a single plant is well documented, but cross-species RNA transfer is largely unexplored. ... This makes perfect sense: "We hypothesized that disulfide bonds, by increasing protein stability, should make proteins' ... develop and apply MAGE-seq to identify RNA structures that determine optimal codon preferences in an essential E. coli gene. ... is a parasitic plant that forms symplastic connections with its hosts and takes up host messenger RNAs (mRNAs). We sequenced ...
  https://www.scoop.it/t/bioinformatics-comparative-genomics-and-molecular-evolution/p/4005731099/2013/08/06/giant-viruses-open-pandora-s-box
*  Smaug (protein) - Wikipedia
Bashirullah, A.; Cooperstock, R. L.; Lipshitz, H. D. (2001-06-19). "Spatial and temporal control of RNA stability". Proceedings ... Smaug is a RNA binding protein in Drosophila that helps in maternal to zygotic transition (MZT). Named after the fictional ... Dahanukar, A.; Walker, J. A.; Wharton, R. P. (August 1999). "Smaug, a novel RNA-binding protein that operates a translational ... Schier, Alexander F. (2007-04-20). "The maternal-zygotic transition: death and birth of RNAs". Science. 316 (5823): 406-407. ...
  https://en.wikipedia.org/wiki/Smaug_(protein)
*  SciCombinator - Systematic discovery of structural elements governing stability of mammalian messenger RNAs.
Systematic discovery of structural elements governing stability of mammalian messenger RNAs.'. ... RNA Stability, RNA, Messenger, RNA, Small Interfering, Time Factors, Transcription, Genetic. ... RNA splicing, Intron, Secondary structure, Cis-regulatory element, Gene expression, DNA, Messenger RNA, RNA MeSH headings. 3' ... Systematic discovery of structural elements governing stability of mammalian messenger RNAs. OPEN Nature , 12 Apr 2012 ...
  http://scicombinator.com/articles/16666
*  Lactococcus lactis peptidoglycane
Previous message: synthetic RNA stability/degradation *Next message: BeadBeater vs. The Sonicator ...
  http://www.bio.net/bionet/mm/methods/1994-December/022102.html
*  Xpert search results for Heuristic local search tutorial
Understand RNA splicing and explain its role in regulating gene expression. Describe the importance of RNA stability in […]. ...
  https://www.nottingham.ac.uk/xpert/scoreresults.php?keywords=Heuristic%20local%20search%20tutorial&start=1500&end=1520
*  Patent US6677606 - Dopa and dopamine modification of metal oxide semiconductors, method for ... - Google Patents
In Splicing, RNA Processing, or RNA Stability. Nanocrystalline titania films on glass mesh or other supports modified with DNA ... Stability of the construct is larger than the stability of a complexes between TiO2 and glycidil, glycidil isopropyl ether, ... or RNA of defined sequence will selectively bind proteins that recognize the sequence of RNA and participate in splicing, RNA ... Selectivity (stringency) of hybridization can be adjusted to the thermal stability of each individual oligonucleotide and ...
  http://www.google.com/patents/US6677606?dq=inassignee:doubleclick
*  RNA modification - Wikipedia
... localization as well as stability of RNAs, and has been linked with human diseases. More than 100 types of RNA modifications ... RNA modification occurs in all living organisms, and is one of the most evolutionarily conserved properties of RNAs. It can ... To identify diverse post-transcriptional modifications of RNA molecules and determine the transcriptome-wide landscape of RNA ... 2013). "NSun2-mediated cytosine-5 methylation of vault noncoding RNA determines its processing into regulatory small RNAs". ...
  https://en.wikipedia.org/wiki/RNA_modification
*  YTHDF2 - Wikipedia
"N6-methyladenosine-dependent regulation of messenger RNA stability". Nature. 505 (7481): 117-20. doi:10.1038/nature12730. PMC ... YTH N6-methyladenosine RNA binding protein 2 is a protein that in humans is encoded by the YTHDF2 gene. This gene encodes a ... Tirumuru N, Zhao BS, Lu W, Lu Z, He C, Wu L (2016). "N(6)-methyladenosine of HIV-1 RNA regulates viral infection and HIV-1 Gag ... The YTH domain is usually located in the middle of the protein sequence and may function in binding to RNA. In addition to a ...
  https://en.wikipedia.org/wiki/YTHDF2
*  Chuan He - Wikipedia
"N6-Methyladenosine-dependent regulation of messenger RNA stability". Nature. 505 (7481): 117-120. Bibcode:2014Natur.505..117W. ... Chuan He is best known for his work in discovering and deciphering reversible RNA methylation in post-transcriptional gene ... He and co-workers identified and characterized the direct reader proteins for m6A, which impact the stability and the ... In 2010, He proposed that RNA modifications could be reversible and may have regulatory roles analogous to well-known ...
  https://en.wikipedia.org/wiki/Chuan_He
*  Calendar : CRM
Modelling RNA stability for decoding the regulatory programs that drive human diseases. Hamed S. Najafabadi, McGill University ... Sasaki-Einstein metrics and K-stability. Tristan Collins, Harvard University. Web site : http://www.math.mcgill.ca/gas/ back to ...
  http://www.crm.math.ca/cal/en/mois201801.html