Semi-quantitative RT-PCR (QRT-PCR) confirmation of puta | Open-i
Reverse Transcriptase Polymerase Chain Reaction/methods. *Signal Transduction/physiology. *Trans-Activators/physiology ... Reverse transcription was performed on the indicated amounts of total RNA, followed by PCR using primers designed for each ... Reverse transcription was performed on the indicated amounts of total RNA, followed by PCR using primers designed for each ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC425575_1471-213X-4-6-2&req=4
Expression Profiling of the Whole Arabidopsis Shaggy-Like Kinase Multigene Family by Real-Time Reverse Transcriptase-Polymerase...
... of the Whole Arabidopsis Shaggy-Like Kinase Multigene Family by Real-Time Reverse Transcriptase-Polymerase Chain Reaction. ... 2000) Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol ... of the family has been performed using the technique of real-time quantitative reverse transcriptase-polymerase chain reaction ... of the Whole Arabidopsis Shaggy-Like Kinase Multigene Family by Real-Time Reverse Transcriptase-Polymerase Chain Reaction ...http://www.plantphysiol.org/content/130/2/577.full
Sensitivity and specificity of a real-time reverse transcriptase polymerase chain reaction detecting feline coronavirus...
Sensitivity and specificity of a real-time reverse transcriptase polymerase chain reaction detecting feline coronavirus ... aim of this study was to evaluate sensitivity and specificity of a real-time reverse transcriptase polymerase chain reaction ( ...https://www.physiciansweekly.com/sensitivity-and-specificity-of-a-real-time-reverse-transcriptase-polymerase-chain-reaction-detecting-feline-coronavirus-mutations-in-effusion-and-serumplasma-of-cats-to-diagnose-feline-infectious-per/
Reverse Transcriptase Polymerase Chain Reaction | Anatomical Neuropharmacology Unit
This is an historical archive of the activities of the MRC Anatomical Neuropharmacology Unit (MRC ANU) that operated at the University of Oxford from 1985 until March 2015. The MRC ANU established a reputation for world-leading research on the brain, for training new generations of scientists, and for engaging the general public in neuroscience. The successes of the MRC ANU are now built upon at the MRC Brain Network Dynamics Unit at the University of Oxford.. ...http://mrcanu.pharm.ox.ac.uk/research-and-techniques/reverse-transcriptase-polymerase-chain-reaction
Browsing SOM Faculty and Staff Research by Subject "Reverse Transcriptase Polymerase Chain Reaction"
... ... Browsing SOM Faculty and Staff Research by Subject "Reverse Transcriptase Polymerase Chain Reaction". Archie Digital ...http://archie.kumc.edu/handle/2271/577/browse?value=Reverse+Transcriptase+Polymerase+Chain+Reaction&type=subject
Prolonged measles virus shedding in human immunodeficiency virus-infected children, detected by reverse transcriptase-...
A reverse transcriptase-polymerase chain reaction assay was used to detect measles virus RNA in peripheral blood mononuclear ... Reverse Transcriptase Polymerase Chain Reaction, Support, Non-U.S. Gov't, Support, U.S. Gov't, P.H.S., Time Factors, Urine, ... detected by reverse transcriptase- polymerase chain reaction. The Journal of infectious diseases, 183 (4). pp. 532-8. ISSN 0022 ... detected by reverse transcriptase- polymerase chain reaction ...http://researchonline.lshtm.ac.uk/16909/
Semiquantitative reverse transcriptase polymerase chain reaction. Oligonucleotide primers (Tyndale et al., 1994) for α4 (503 bp ... Assessment of α4 mRNA levels after 3α,5α-THP withdrawal with semiquantitative reverse transcriptase polymerase chain reaction ( ... were housed in groups of three under a reverse light/dark cycle (14/10 hr light/dark). Food and water were available ad libitum ... Total RNA was isolated using Tri reagent and a chloroform extraction and was followed by reverse transcription (SuperScript II ...http://www.jneurosci.org/content/18/14/5275
Regulation of 6-Pyruvoyltetrahydropterin Synthase Activity and Messenger RNA Abundance in Human Vascular Endothelial Cells |...
Reverse Transcriptase-Polymerase Chain Reaction. The relative expression of GTPCH, PTPS, cNOS, and iNOS mRNAs compared with the ... Reverse-transcription/limiting-dilution polymerase chain reaction analysis showed that in response to IFN/TNF/IL-1, mRNA ... and first-strand cDNA was used as a template in polymerase chain reaction (PCR). cDNA aliquots were amplified with the ... For comparative determination of mRNA levels, limiting-dilution PCRs of cDNA were performed.13 14 After reverse transcription ( ...http://circ.ahajournals.org/content/98/17/1703
Heat-Shock Protein 60-Reactive CD4+CD28null T Cells in Patients With Acute Coronary Syndromes | Circulation
Reverse-Transcriptase Polymerase Chain Reaction. Cellular RNA was extracted with Trizol reagent and 20 μg glycogen followed by ... The CD4+CD28null phenotype was further confirmed by reverse-transcriptase polymerase chain reaction (RT-PCR) analysis of mRNA ... Detection of cell specific cluster determinant expression by reverse transcriptase polymerase chain reaction. J Immunol Methods ... cDNA synthesis with Superscript II reverse transcriptase and random hexamers (Life Technologies). Amplification of IFN-γ and ...http://circ.ahajournals.org/content/109/10/1230.long
Enhanced Lymph Vessel Density, Remodeling, and Inflammation Are Reflected by Gene Expression Signatures in Dermal Lymphatic...
Quantitative reverse transcriptase-polymerase chain reaction.. One microgram of amplified total RNA was transcribed into cDNA ... by Superscript II Reverse Transcriptase (Invitrogen no. 18064-014). A complete probe list is summarized in Supplementary Table ... ndLECs were selected, and qPCR reactions were performed in triplicate, with total RNA extracted from dLECs and ndLECs (n = 4 in ... TNF-α-treated LEC monolayers showed twofold-enhanced macrophage adhesion in vitro, which could be completely reversed by anti- ...http://diabetes.diabetesjournals.org/content/62/7/2509.full
Reverse Transcriptase-Polymerase Chain Reaction.. Total RNA was extracted from AdhPEPT1-EYFP-transduced cells and frozen ... reverse transcription-polymerase chain reaction. PBS. phosphate-buffered saline. EYFP. enhanced yellow fluorescent protein. ... Total RNA was reverse-transcribed and amplified by polymerase chain reaction (PCR) by using specific primers for hPEPT1-EYFP. ... RT (−) represents the control lacking reverse transcriptase enzyme. B, Fluorescence (a) and phase-contrast (b) micrographs of ...http://jpet.aspetjournals.org/content/305/1/40
Tranilast Suppresses Vascular Chymase Expression and Neointima Formation in Balloon-Injured Dog Carotid Artery | Circulation
Quantitative Reverse-Transcriptase Polymerase Chain Reaction Analysis. Chymase and ACE mRNA levels of carotid arteries were ... determined with quantitative reverse-transcriptase polymerase chain reaction (PCR) analysis described previously with ... polymerase chain reaction. Percutaneous transluminal coronary angioplasty (PTCA) is an established therapy for obstructive ... acetonitrile and 10 μL of acetic acid and then fractionated by reverse-phase high-performance liquid chromatography. Common ...http://circ.ahajournals.org/content/99/8/1084
Myosin II regulates extension, growth and patterning in the mammalian cochlear duct | Development
Reverse transcriptase polymerase chain reaction. Epithelia from five E13.5 or E16.5 cochleae were isolated by treating with ... a pair of regulatory light chains (RLCs) and a pair of essential light chains. Phosphorylation of the RLCs by a number of ... Kamm, K. E. and Stull, J. T. (1985). The function of myosin and myosin light chain kinase phosphorylation in smooth muscle. ... Wadgaonkar, R., Linz-McGillem, L., Zaiman, A. L. and Garcia, J. G. (2005). Endothelial cell myosin light chain kinase (MLCK) ...http://dev.biologists.org/content/136/12/1977
Efferent Vagal Fibre Stimulation Blunts Nuclear Factor-κB Activation and Protects Against Hypovolemic Hemorrhagic Shock |...
RNA Isolation and Reverse Transcriptase-Polymerase Chain Reaction. Total cellular RNA was extracted6 from liver section 20 ... Nuclear factor κB (NF-κB) is an ubiquitous rapid response transcription factor involved in inflammatory reactions and exerts ...http://circ.ahajournals.org/content/107/8/1189.long
reverse transcriptase-polymerase chain reaction. BrdU. 5′-bromo-2′-deoxy-uridine. MTT. 3-(4,5-cimethylthiazol-2-yl)-2,5- ... Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR).. Total RNA was isolated from PC12 cells by using the method of ... 1997) Glucagon-like peptide-1 can reverse the age-related decline in glucose tolerance in rats. J Clin Invest 99:2883-2889. ... Our findings suggest that such peptides may be used in reversing or halting the neurodegenerative process observed in ...http://jpet.aspetjournals.org/content/300/3/958
p38 maintains E-cadherin expression by modulating TAK1-NF-κB during epithelial-to-mesenchymal transition | Journal of Cell...
Reverse-transcriptase polymerase chain reaction. Total RNA was extracted with the RNeasy kit (Qiagen, Hilden, Germany), and ...http://jcs.biologists.org/content/123/24/4321
Identification of Genes Potentially Involved in Rupture of Human Atherosclerotic Plaques | Circulation Research
Reverse Transcriptase-Polymerase Chain Reaction. Isolation of total RNA was carried out as described above. The SMART PCR cDNA ... Reverse transcriptase-polymerase chain reaction analysis on 10 individual ruptured and 10 individual stable plaques showed a ... To validate the reproducibility of expression of these clones, reverse transcriptase (RT)-polymerase chain reaction (PCR) ... In the forward reaction, genes upregulated in ruptured plaques were isolated, whereas the reverse reaction resulted in the ...http://circres.ahajournals.org/content/89/6/547
Role of pulmonary intravascular macrophages in endotoxin-induced lung inflammation and mortality in a rat model | Springer for...
Reverse transcriptase-polymerase chain reaction. Total RNA was extracted from lungs of three rats from each group tissues of ... Each reaction was performed using 10 ng of total RNA and thermocycler was programmed for reverse-transcription at 55°C for 30 ... A negative control reaction consisted of all the components of the reaction mixture except RNA. Amplified PCR products were ... On color development, reaction was stopped by adding 50:l of 0.5 M sulfuric acid. Microplates were read at 450 nm. ...https://rd.springer.com/article/10.1186/1465-9921-9-69
Temporal profile of Src, SSeCKS, and angiogenic factors after focal cerebral ischemia: correlations with angiogenesis and...
Reverse transcriptase-polymerase chain reaction. Total RNA from ipsilateral ischemic cerebral cortex (n=6/group) was extracted ... Reverse transcriptase-polymerase chain reaction and Western blotting results revealed that Src gradually increased starting as ... Then 2 μl of the reverse transcription reaction was amplified by polymerase chain reaction (PCR) in a volume of 20 μl with gene ... reverse: 5′-ATA CAC TAT CTC ATC GGG GTA CT-3′ Ang-1 forward: 5′-GAA AAT TAT ACT CAG TGG CTG GAA AAA-3′ reverse: 5′-TTC TAG GAT ...http://pubmedcentralcanada.ca/pmcc/articles/PMC3100427/?lang=en-ca
Serial analysis of gene expression in the frontal cortex of patients with bipolar disorder | The British Journal of Psychiatry
Reverse transcriptase-polymerase chain reactions. The reverse transcriptase-polymerase chain reaction (RT-PCR) technique was ... Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of NF-κB1, NF-κB3 and IκB using cDNA from the frontal cortex ... Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of differentially expressed genes in the frontal cortex ... Method Serial analysis of gene expression (SAGE) and reverse transcriptase-polymerase chain reaction were used to identify RNA ...http://bjp.rcpsych.org/content/178/41/s137
Customized Ca-P/PHBV nanocomposite scaffolds for bone tissue engineering: design, fabrication, surface modification and...
Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. After 21-day induction in the osteogenic medium, total RNA ... and 2 µL of cDNA template from each RT reaction were subjected to PCR reactions using FastStart Taq DNA polymerase (Roche ... The reaction was stopped by the addition of 3 N NaOH and then the production of p-nitrophenol in the presence of ALPase was ... The mRNA was reverse transcribed to complementary DNA (cDNA) using a Transcriptor First Strand cDNA Synthesis Kit (Roche ...http://rsif.royalsocietypublishing.org/content/early/2010/05/20/rsif.2010.0127.focus
Expression of c-Kit isoforms in multiple myeloma: differences in signaling and drug sensitivity | Haematologica
Reverse transcriptase polymerase chain reaction (RTPCR) assay. RNA was isolated from MM1S cells, and an equal amount (2 μg) ... Design and Methods Expression of c-Kit isoforms was investigated by reverse transcriptase polymerase chain reaction in fresh ... from the different samples was primed with poly-T. cDNA was synthesized with M-MLV reverse transcriptase (RT; Promega). The ...http://www.haematologica.org/content/93/6/851
TREM-1 Mediates Inflammatory Injury and Cardiac Remodeling Following Myocardial InfarctionNovelty and Significance |...
Quantitative Reverse Transcriptase-Polymerase Chain Reaction. Total RNAs were extracted from myocardium (infarcted or remote ... infarcted and noninfarcted areas after MI in mice were analyzed by quantitative reverse transcriptase-polymerase chain reaction ... and quantified by quantitative polymerase chain reaction (PCR) using Qiagen available probes (Quantitect Primers) for Trem-1, ... Myocardial infarction (MI) triggers an intense inflammatory reaction whose timely resolution is crucial to minimize cardiac ...http://circres.ahajournals.org/content/116/11/1772
Evidence for the Involvement of Type I Interferon in Pulmonary Arterial HypertensionNovelty and Significance | Circulation...
Quantitative Reverse Transcriptase Polymerase Chain Reaction. Quantitative reverse transcriptase polymerase chain reaction for ... Gene and protein expression were measured using reverse transcriptase polymerase chain reaction, Western blotting, and ...http://circres.ahajournals.org/content/114/4/677
Accumulation of hypoxia-inducible factor-1α protein and its role in the differentiation of myeloid leukemic cells induced by...
Real-time quantitative reverse transcriptase polymerase chain reaction. Total cellular RNA was extracted from cells using ... α/HIF-1β mRNA and proteins were measured respectively by real-time quantitative reverse transcriptase polymerase chain reaction ... 5 and reverse the dominant-negative effects on functions of the wild type PML and RARα proteins of the PML-RARα oncoprotein, ... TRIzol reagent (Invitrogen, Carlsbad, CA, USA) and reverse transcription was performed using the cDNA synthesis kit according ...http://www.haematologica.org/content/93/10/1480
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