Engineering DNA Backbone Interactions Results in TALE Scaffolds with Enhanced 5-Methylcytosine Selectivity | Scientific Reports
TALEs contain an N-terminal region (NTR) and a central repeat domain (CRD). Repeats of the CRD selectively recognize each one ... However, both TALE domains also nonspecifically interact with DNA phosphates via basic amino acids. To enhance the 5mC ... We then analysed conserved, DNA phosphate-binding KQ diresidues in CRD repeats and identified further improved mutants. ... Combination of mutations in the NTR and CRD was highly synergetic and resulted in TALE scaffolds with up to 4.3-fold increased ...
Viruses | Special Issue : In Memory of Michael Rossmann
Concomitantly, bile alters the conformation of the P domain itself from one that binds antibodies to one that recognizes ... SS, signal sequence; NTD, N-terminal domain; CTD1/2/3, C-terminal domains 1/2/3; RBD: receptor binding domain; S1/S2: S1/S2 ... These binding sites often correspond to "weak points" on the capsid lattice. Because of their unique abilities to bind virus ... domain that is loosely attached to the shell via a single-polypeptide tether. Small molecules found in the gut, such as bile ...
Paratope Duality and Gullying are Among the Atypical Recognition Mechanisms Used by a Trio of Nanobodies to Differentiate...
We had previously shown that three anti-Marburg virus nanobodies (VHH or single-domain antibody [sdAb]) targeted a cryptotope ... The Sudan ebolavirus-specific sdAb was more remarkable and appeared to bind two C-terminal domains simultaneously via ... Binding and X-ray crystallographic studies revealed that the primary determinant of cross-reactivity did indeed appear to be a ... The varied routes used by sdAbs to engage antigens discovered here deepen our appreciation of the small scaffolds ...
Bispecific monoclonal antibody - Wikipedia
August 1988). "Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv ... Bispecific monoclonal antibody entry in the public domain NCI Dictionary of Cancer Terms Bispecific+antibodies at the U.S. ... A bispecific monoclonal antibody (BsMAb, BsAb) is an artificial protein that can simultaneously bind to two different types of ... April 2010). "Introducing antigen-binding sites in structural loops of immunoglobulin constant domains: Fc fragments with ...
EUROpean consortium on NEUTralising antibodies using gp41 | EURONEUT-41 | Project | News & Multimedia | FP7 | CORDIS | European...
9.4 Passive immunization with anti-PID antibodies. The principal immunodominant domain of gp41, PID, is able to induce binding ... Despite this short-lived exposure, the CHR and NHR regions can be accessed by small proteins, peptides and antibodies that bind ... gp41 is divided into three major domains: the extracellular region or ectodomain (aa 1 to 171), the transmembrane (TM) domain ( ... MPER neutralisation is not dominated by any single specificity akin to known MPER-specific monoclonal antibodies. Presented in ...
Frontiers | Dissecting the Role of BET Bromodomain Proteins BRD2 and BRD4 in Human NK Cell Function
B) Binding affinities of single (JQ1) and tandem (AZD5153) BET inhibitors previously determined in a Fluorescent polarization ( ... Bromodomains are proteins that contain modules of ~110 amino acids that recognize and bind acetylated lysine residues in ... antibodies. The ChIP experiment was then performed for each antibody as described previously by Orlando et al. (24). Next, ... A) Domain organization of BET (BRD2,3,4) proteins highlighting the N-terminal tandem bromodomain (BRD) modules, the Extra- ...
The RNA-binding proteomes from yeast to man harbour conserved enigmRBPs | Nature Communications
RNA-binding proteins (RBPs) are implicated in many biological functions. Here the authors expand the human and yeast RNA ... Analyses of the mitochondrial hydroxysteroid dehydrogenase (HSD17B10) uncover the RNA-binding specificity of an enigmRBP. ... of the human RBPs have functions assigned to RNA biology or structural motifs known to convey RNA binding, and many intensively ... several of which with no prior function assigned to RNA biology or structural motifs known to mediate RNA-binding, and ...
Suzanne Pfeffer's Profile | Stanford Profiles
The crystal structure of Rab6 bound to the GCC185 Rab-binding domain reveals that Rab6 recognizes a two-fold symmetric surface ... Monoclonal antibodies that recognize cytoplasmic domains of these polypeptides can penetrate the clathrin coat and recognize ... Rabs organize distinct protein scaffolds within a single organelle and act in a combinatorial manner with their effectors to ... TIP47 recognizes distinct determinants in the cytoplasmic domains of these two receptors, and its ability to bind to the cation ...
King Lab | Publications
Epitopes of ebolavirus glycoprotein (GP) recognized by antibodies with binding breadth for all three ebolaviruses are of major ... comprising the SARS-CoV-2 spike protein receptor-binding domain displayed on an I53-50 protein nanoparticle scaffold (hereafter ... The nanoparticle vaccines display 60 SARS-CoV-2 spike receptor-binding domains (RBDs) in a highly immunogenic array and induce ... This approach allowed to resolve structurally distinct classes of antibodies that bind overlapping sites. In addition to ...
Anti V5-Tag Antibody, clone SV5-Pk1 | Bio-Rad
... recognizes the sequence, IPNPLLGL, on recombinant proteins, including transmembrane and secreted proteins tagged with IPNPLLGL. ... Dunn, C. et al. (1999) Fine mapping of the binding sites of monoclonal antibodies raised against the Pk tag. J Immunol Methods ... Mouse anti V5 tag antibody, clone SV5-Pk1 (MCA1360) used to bind the SV5 tag on recombinant protein fragments by immunoblotting ... and schematic showing the M-domain of 53BP1. Lysine 1568 (white circle) lies within the second Tudor domain and lysine 1690 ( ...
Maria Monti - CIRN (Centro InterUniversitario di Ricerca in Neuroscienze)
Two novel human antitumor immunoconjugates, engineered by fusion of a single-chain antibody fragment against human ErbB2 ... EDIAs bind to a different ErbB2 epitope than Herceptin and the other human/humanized antibodies against ErbB2 reported so far. ... This amyloid layer acts as a molecular scaffold that promotes localised melanin synthesis and the adhesion of immune cells ... The epitope has been successfully located in region 122-195 of extracellular domain I. These findings could lead to the ...
Pesquisa | Biblioteca Virtual em Saúde - BRASIL
... the construction of synthetic antibody libraries based on a single engineered human autonomous variable heavy domain scaffold ... display selection on cells is a powerful tool to generate highly specific antibodies recognizing a target in its cell bound ... But it is difficult to direct recombinant antibodies to bind either to- or near a specific amino acid on a protein or peptide. ... The developed selection system has shown to be highly versatile and has the potential to be applied to other binding domains ...
Publications
Selective inhibition of matrix metalloproteinase 10 (MMP10) with a single-domain antibody.. J Biol Chem. 2020 Jan 17. pii: jbc. ... The mechanism of peptide-binding specificity of IAP BIR domains.. Cell Death Differ. 2008 Feb 1; PMID: 18239672 Denault JB, ... The Human Anti-apoptotic Proteins cIAP1 and cIAP2 Bind but Do Not Inhibit Caspases J. Biol. Chem. 2006 281: 3254-3260. First ... Resurrection of an ancient inflammatory locus reveals switch to caspase-1 specificity on a caspase-4 scaffold.. J Biol Chem. ...
CIPSM - 2009
These proteins contain two related domains known as the chromo- and the chromoshadow-domain. The chromo-domain binds histone H3 ... Here we report the selection of camelid-derived single-domain antibodies (nanobodies) that modulate the conformation and ... domain of the nucleosome re-assembly factor Spt6. This SH2 domain is unusual in its specificity to bind phosphoserine, rather ... Once mostly regarded as dull membrane anchors, transmembrane domains are now recognized as major instigators of protein-protein ...
The Maguk protein, Pals1, functions as an adapter, linking mammalian homologues of Crumbs and Discs Lost | Journal of Cell...
... proteins are scaffold proteins that contain PSD-95-Discs Large-zona occludens-1 (PDZ), Src homology 3, and guanylat ... domains, a PDZ domain, SH3 domain, protein 4.1 binding (4.1B) domain, and GUK domain. (B) MDCK cells were immunostained as ... Immunoblotting with antibodies raised against the COOH terminus of Pals1 recognizes two bands at ∼71 kD (Fig. 8 D), suggesting ... we wondered if the Discs Lost MRE domain functioned similarly by binding to the single L27 domain of CG1617. In Fig. 9 C, we ...
Epithelial Sodium Channels
Note that antibodies AH12.2 and 6D8 recognize distinct domains of Dsg2. (D) Schematic epitope diagram of the Dsg2-specific ... scaffold and model framework for the design and synthesis of several pharmacological compounds having significant binding ... C) AH12.2 recognizes the initial extracellular domain name of Dsg2. CHO cells were transfected with constructs encoding ... We have shown that antibodies bind to the surface of UPEC expressing the antigens. Sera from ladies with and without histories ...
Publications - Institute for Bioengineering of Catalonia
... has been merged with the structure of the antibody CR3022 Fab tightly bounded to SARS-CoV-2 receptor-binding domain (RBD) and ... Neutralizing antibodies, Physiological condition, Physiology, Receptor-binding domains, Therapeutic modality, Viruses ... The binary dispersion was characterized by a single structural relaxation, a single Johari-Goldstein process, and two local ... is strongly influenced by the architecture of the pore network of the scaffold. JTD Keywords: Scaffolds, Microstructure, Cell ...
Advances in the Pathogenesis of Auto-antibody-Induced Cerebellar Synaptopathies | The Cerebellum
The presence of auto-antibodies that target synaptic machinery proteins was documented recently in immune-mediated cerebellar ... Human monoclonal GAD65Ab b78 recognizes an epitope that is recognized by GAD65Ab in patients with CA, whereas human monoclonal ... Actions of anti-GluR delta Ab were examined using monoclonal Ab against H2 ligand binding site of GluR delta, and not using Abs ... This should be extended not only to motor control studies, but also to cognitive, affective, and social domains. Fifth, the ...
Antibody - Wikipedia
Some antibodies form complexes that bind to multiple antigen molecules.. Secreted antibodies can occur as a single Y-shaped ... One of these domains is called the variable domain, which is present in each heavy and light chain of every antibody, but can ... To allow the immune system to recognize millions of different antigens, the antigen-binding sites at both tips of the antibody ... Gebauer M, Skerra A (June 2009). "Engineered protein scaffolds as next-generation antibody therapeutics". Current Opinion in ...
Plus it
... is reported to bind group II WW domains through its proline rich domain through the canonical PPLP binding motif (Muthusamy et ... D , Immunoprecipitation of 4 h continuously labeled cell lysates using antibody 26D6, which recognizes the N-terminal region of ... may act as a scaffold between APP and neurexins as Mint1 has been shown to bind to APP via a phosphotyrosine binding domain ( ... Additionally, we report the identification of novel APP binding protein, NEEP21, a neuronally expressed single-pass ...
Synthesis and Applications of Synthetic Peptides | IntechOpen
... production of antibodies, and vaccine design. The use of synthetic peptides approved by the health authorities for vaccine, for ... is carried out by using peptide-bound tetradentate 99Tcm chelators (N3S or N4 scaffold). The peptides are coordinated with ... In order to bind the first amino acid to the resins, chemical structures containing functional group(s) called linker. are ... The critical step in the barrel-stave model is that the peptides recognize each other while they are attached to the membrane. ...
Integrin Targeted Therapeutics
Rapid, immune-mediated platelet destruction occurs through the binding of IgG and IgM antibodies that recognize murine ... The extracellular domain protrudes from the cell surface to interact with and bind, in a divalent cation-dependent manner, ... Phase II studies of cilengitide as a single agent and in combination. At least five Phase II trials of cilengitide as a single ... ILK associates with actin through its binding partner parvin creating a core scaffold for focal adhesion formation. Focal ...
Chemical Modifications of Aptamers | Encyclopedia MDPI
... via SELMA selection could recognize the monoclonal antibody, 2G12, which is known to bind to mannose-rich glycans on the HIV ... Aptamers are single-stranded RNA or DNA oligonucleotides that fold up into a distinctive 3D structure capable of binding with ... Bel, N.; Das, A.T.; Berkhout, B. In Vivo SELEX of single-stranded domains in the HIV-1 leader RNA. J. Virol. 2014, 88, 1870- ... small molecule drugs and antibodies can be achieved if these molecules can fold into unique 3-D structure scaffolds to bind ...
A highly compact epitope-based marker/suicide gene for easier and safer T-cell therapy | Blood | American Society of Hematology
Antibody binding vs eGFP expression is shown. Q8 binds neither mAb; CD20 binds both, whereas RQR8 only binds rituximab. (B) ... Our final construct, RQR8, is a 136-amino-acid protein that is recognized by the anti-CD34 antibody QBEnd10, the antibody used ... Both antibodies could bind in either orientation. (C) Sensitivity to CDC depletion using primary human T cells transduced with ... QBEnd10 binding cells were single-cell sorted and the retroviral integrant subsequently sequenced from genomic DNA (not shown ...
December | 2013 | Histamine Receptor Signaling
... and simply because weve got injected 3 numerous anti bodies, as well as a newly developed a single towards the P MCAK protein ... A class of G protein-coupled receptors which bind histamine as their primary endogenous ligand.. Search. Main menu. Skip to ... The TIR domain is extremely conserved in insect and mammalian Toll families and features a much more reputable deter mination ... With this particular in thoughts, we constructed a phylo genetic tree using the TIR domains making use of the plan Mega 5. 05. ...
Supramolecular assembly of protein building blocks: from folding to function | Nano Convergence | Full Text
These assemblies are widely used in biology as they enable multivalent binding, ultra-sensitive regulation, and ... A single building block is sufficient to create homogeneous structures with complex shapes, such as rings, filaments, or ... C The octahedral (up) and icosahedral (down) antibody nanocages with fusion of angiopoietin-1 F-domain (A1F) and Fc (Image ... has been utilized as a scaffold for cell growth [158]. These proteins utilize cell-binding epitopes for binding to integrins, ...
Frontiers | Plant 3' Regulatory Regions From mRNA-Encoding Genes and Their Uses to Modulate Expression
In addition, after transcription, a wide array of RNA-binding proteins interacts with cis-acting elements located mainly in the ... In addition, after transcription, a wide array of RNA-binding proteins interacts with cis-acting elements located mainly in the ... Expression and Characterization of a Recombinant Single-Domain Monoclonal Antibody Against MUC1 Mucin in Tobacco Plants. ... but functions as a scaffold to preorganize two other subunits, CPSF30 and WDR33, which synergistically bind to the hexamer with ...
New Products | MJS BioLynx Inc.
EpiCypher - New Antibodies: SNAP-Certified™ for CUT&RUN and YEATS Domain Proteins. Good news for epigenetics researchers! ... Finneran Porvair - High Bind & Medium Bind Plates for Your COVID-19 Testing. In the critical research and development of COVID- ... Medium and High binding surfaces to suit different assays. Click here for further information.. Click here to request a quote. ... and two new YEATS domain proteins (YEATS2 and GAS41 domains)! ... 3D CoSeedis™ is a novel scaffold-free 3D cell co-culture system ...
Artificial Intelligence in Medicine - Applications in Therapeutics | Leaders in Pharmaceutical Business Intelligence (LPBI)...
... or their binding affinity [5], de novo generation of proteins binding to specific targets (e.g., antibodies [6]), modeling back ... Five binding subsites (A-E) make up the carbohydrate-recognition domains of these galectins. We identified novel interactions ... 3] Protein-protein and domain-domain interactions, Kangueane and Nilofer, 2018. [4] Side-chain Packing Using SE(3)-Transformer ... Recognize the future implications for AI, plus the benefits and obstacles that may come from this new process. ...