3T3-L1 cells). KSS inhibited the accumulation of triglycerides in a dose-dependent manner in 3T3-L1 cells that were induced to ... 3T3-L1 cells). KSS inhibited the accumulation of triglycerides in a dose-dependent manner in 3T3-L1 cells that were induced to ... 3T3-L1 cells). KSS inhibited the accumulation of triglycerides in a dose-dependent manner in 3T3-L1 cells that were induced to ... 3T3-L1 cells). KSS inhibited the accumulation of triglycerides in a dose-dependent manner in 3T3-L1 cells that were induced to ...
Receptor for advanced glycation end products (RAGE) has been shown to be involved in adiposity as well as atherosclerosis even ... 3T3-L1 cell culture and differentiation.. 3T3-L1 preadipocytes were cultured in Dulbeccos modified Eagles medium (DMEM; ... When 3T3-L1 cells were infected with Ad-RAGE, and the cells were induced to differentiate for 6 days, significant numbers of ... or Ad-RAGE-infected 3T3-L1 cells were stained for 1 h with 0.5 μmol/L BODIPY to analyze the proportion of lipid-laden cells ...
Western blotting. 3T3-L1 cells were treated as described, and whole-cell extracts were made in RIPA buffer. Equal amounts of ... OLR1 is induced by PPARγ ligands in 3T3 L1 adipocytes. (A) Comparison of OLR1 and aP2 mRNA levels in 3T3-L1 preadipocytes ( ... cells were treated with appropriate reagents (see figure legends) for 48 hours. Total RNA was extracted from 3T3-L1 cells, ... Orlicky, DJ, Schaack, J. Adenovirus transduction of 3T3-L1 cells. J. Lipid Res. 2001. 42:460-466. View this article via: PubMed ...
"Identification of novel putative membrane proteins selectively expressed during adipose conversion of 3T3-L1 cells". ... and also on the cell membrane, which indicates that MC2 needs MRAP to reach the cell membrane. In addition to cell trafficking ... Unlike MC2, MRAP is not essential for these receptors as they were located on the cell surface in the absence of MRAP1. The ... MRAP assists in the transport of the melanocortin 2 receptor to the cell membrane from the endoplasmic reticulum and assist in ...
... is manifested as attenuated insulin receptor (IR) signaling in response to insulin binding. A drug ... Whole-cell IR autophosphorylation.. Either differentiated 3T3-L1 adipocytes or CHO cells expressing wild type and a mutant IR ... TLK16998 increases IR signaling in 3T3-L1 cells.. To explore the effect of TLK16998 on insulin action in cells, differentiated ... 3T3-L1 adipocytes were differentiated as above on a microscope chamber slide. The cells were serum-starved for 16 h and ...
VN extract suppressed adipocyte differentiation and significantly attenuated the expression of adipogenic genes in 3T3-L1 cells ... on apoptosis in estrogen receptor-negative MDA-MB-231 human breast cancer cells. The LMWF treatment of MDA-MB-231 cells was ... cells were used as target cells. A live cell suspension of Karenia mikimotoi (SUO-1) induced the release of LDH from these cell ... The RSa cells were more sensitive to UVB lethality than the APr-1 cells. Overproduction of annexin II in RSa cells resulted in ...
... insulin receptor substrate 1 (IRS-1) is essential for metabolic regulation. A long and unstructured C-terminal region of IRS-1 ... Insulin and insulin-like growth factor 1 stimulate the phosphorylation on tyrosine of a 160 kDa cytosolic protein in 3T3-L1 ... cells were sorted into single cells by flow cytometric cell sorting and seeded into 96-well plates. IRS-1 KO single-cell clones ... The HEK293T cell line was from American Type Culture Collection. C2C12 cells and MCF7 cells were grown in DMEM (high glucose) ...
... prevents induction of peroxisome proliferator-activated receptor gamma-2 and adipose differentiation of 3T3-L1 cells. Biochim ... Journal of cell science. 2012;125:4067-76.. Dalen KT, Dahl T, Holter E, et al. LSDP5 is a PAT protein specifically expressed in ...
Perfluorooctanoic acid binds to peroxisome proliferator-activated receptor γ and promotes adipocyte differentiation in 3T3-L1 ... Furthermore, in rat cell lines, cell death and the activation of the aryl hydrocarbon receptor (AhR) were reported in some ... including peroxisome-proliferator-activated receptors (PPARs), estrogen receptors (ERs), and the pregnane X receptor (PXR); ... elevated renal glucocorticoid receptor expression, elevated aryl hydrocarbon receptor (AhR) activation, increased reactive ...
The expression of insulin receptor substrate-1 (IRS-1) and its phosphorylated form, (p-IRS-1), as well as G6PC2, was determined ... The upregulation of hsa_circ_0046060 decreased the intracellular glucose content in L-02 cells (43.45 vs. 16.87 pM/mg, ,span ... Exosomes secreted by the umbilical cord mesenchymal stromal cells (UMSCs) and circular RNAs (circRNAs) derived from exosomes ... AKT kinase-mediated insulin signaling by O-Linked beta-N-acetylglucosamine in 3T3-L1 adipocytes," Journal of Biological ...
Co-culturing 3T3L-1 adipocytes and mouse macrophage cells showed incubating adipocytes with IS increased macrophage ... 3T3-L1 cells were seeded in a 96-well plate and allowed to differentiate into adipocytes. After cell differentiation, the cells ... MCP-1 induces the infiltration of macrophages into adipose tissue via the C-C chemokine receptor-2 (CCR2), a receptor that is ... 3T3-L1 cells were seeded on the lower layer and differentiated to adipocytes and mouse macrophage cells (RAW264.7) were then ...
... through calpain family protein and peroxisome proliferator-activated receptor gamma signaling in mouse 3T3-L1 cells. Ramalingam ... Suppression of Nrf2 attenuates adipogenesis and decreases FGF21 expression through PPAR gamma in 3T3-L1 cells. Kim BR, Lee GY, ... Long non-coding RNA lnc-OAD is required for adipocyte differentiation in 3T3-L1 preadipocytes. Wang ZG, Luo ZC, Dai Z, Zhong YT ... Enzymatic fragments of hyaluronan inhibit adipocyte differentiation in 3T3-L1 pre-adipocytes. Park BG, Lee CW, Park JW, Cui Y, ...
3T3-L1 Cells, Adenosine Triphosphate, Adipocytes, Adipogenesis, Animals, Cell Division, Cell Survival, Gene Expression ... Piceatannol dose-dependently inhibited differentiation mixture-induced phosphorylation of insulin receptor (IR)/insulin ... We show that piceatannol inhibits adipogenesis of 3T3-L1 preadipocytes in a dose-dependent manner at noncytotoxic ... In the early phase of adipogenesis, piceatannol-treated preadipocytes displayed a delayed cell cycle entry into G(2)/M phase at ...
... peroxisome proliferator-activated receptor γ, CCAAT element binding protein α, and sterol regulatory element binding ... on 3T3-L1 cells.. Design : 3T3-L1 cells were differentiated in the presence of different concentrations of TME. Lipid ... Anti-adipogenic effects of Tropaeolum majus (nasturtium) ethanol extract on 3T3-L1 cells * Gi-Chang Kim ... Changes in the expression of proteins related to adipocyte differentiation in 3T3-L1 cells were determined by SDS-PAGE and ...
The leptin expression was significantly increased in the cells treated with high concentrations (10-5 and 10-6 mol/l) of the ... Role of estrogen receptor-α and -β in regulating leptin expression in 3T3-L1 adipocytes. / Yi, Kyong Wook; Shin, Jung Ho; Seo, ... Role of estrogen receptor-α and -β in regulating leptin expression in 3T3-L1 adipocytes. In: Obesity. 2008 ; Vol. 16, No. 11. ... Role of estrogen receptor-α and -β in regulating leptin expression in 3T3-L1 adipocytes. Obesity. 2008 Nov;16(11):2393-2399. ...
The effect of Cinnamtannin B1 on cell proliferation and glucose uptake of 3T3-L1 cells. Natural Product Communications, 2 (1) ... A Proanthocyanidin From Cinnamomum Zeylanicum Stimulates Phosphorylation Of Insulin Receptor In 3T3-L1 Adipocytes. Jurnal ... Lipid modulation activity of garcinia atroviridis extract on 3T3-L1 cell line. In: 30th Scientific Meeting of Malaysian Society ... Garcinia atroviridis fruit extract alters lipid accumulation in 3T3-L1 cell line model. In: International Conference on Natural ...
J Cell Mol Med 2015;19:2413 * Effects of lipoic acid on lipolysis in 3T3-L1 adipocytes. Authors: Fernández-Galilea Et al. ... Cell Commun Signal 2012;109:9179 * Protection of protease-activated receptor 2 mediated vasodilatation against angiotensin II- ... Prostaglandin receptor EP3 regulates cell proliferation and migration with impact on survival of endometrial cancer patients. ... Prostaglandin E2 receptor 3 signaling is induced in placentas with unexplained recurrent pregnancy losses. Authors: Ye Et al.. ...
... and peroxisome proliferator-activated receptor ?, two main regulators of terminal adipogenesis, in 3T3-L1 cells. The presence ... 3T3-L1) into adipocytes and on the capability of these cells to synthesize estrogens by regulating the expression and enzyme ... Thus, in both differentiating and differentiated 3T3-L1 adipocytes, high concentrations of melatonin increased intracytoplasmic ... thereby reducing the number of estrogen-producing cells proximal to malignant cells. ...
3T3-L1 Cells * Adipocytes, White / drug effects * Adipocytes, White / immunology * Adipocytes, White / metabolism* ... The co-culture of RAW and L1 increased TNF-α, S100B, and the expression of M1 markers and the MCP-1 receptor CCR2. The ... 3T3-L1 adipocytes (L1) were stimulated by TNF-α to examine S100B secretion. RAW and L1 were then mutually stimulated with ... L1 conditioned media stimulated TNF-α secretion from RAW, and RAW conditioned media increased S100B secretion from L1. ...
Comparison of the effects of cannabinoid receptor modulation in human primary preadipocytes and a murine cell line. Agarwal ... We have previously described the effects of CB1 activation/inactivation on murine white preadipocyte 3T3-L1 proliferation; we ... Comparison of the effects of cannabinoid receptor modulation on adipogenesis in human primary preadipocytes and a murine cell ... Potential aetiologies include drugs, critical illness, hormone deficiencies, and non islet cell tumors. In the absence of these ...
Parray, H.A., Yun, J.W. Cannabidiol promotes browning in 3T3-L1 adipocytes. Mol Cell Biochem416, 131-139 (2016). ... The body sends them to signal cells via the cannabinoid receptors. Phytocannabinoids - like CBD or THC - mimic the actions of ... Parray, H. A., & Yun, J. W. (2016). Cannabidiol promotes browning in 3T3-L1 adipocytes. Molecular and cellular biochemistry, ... Brown fat cells demonstrate higher metabolic activity than white cells. They easily burn off energy as heat, prompting ...
... expression in 3T3-L1 cells." in: Journal of cellular biochemistry, Vol. 116, Issue 6, pp. 1153-63, (2015) (PubMed). Zhang, Peng ... peroxisome proliferator-activated receptor gamma (PPARG) ELISA Kits. peroxisome proliferator activated receptor gamma (PPARG) ... PPARG Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum ... PPARG Reactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum ...
Environmental endocrine disruptors promote adipogenesis in the 3T3-L1 cell line through glucocorticoid receptor activation. ... Central role for protein targeting to glycogen in the maintenance of cellular glycogen stores in 3T3-L1 adipocytes. Mol Cell ... Uncoupling of 3T3-L1 gene expression from lipid accumulation during adipogenesis. FEBS Lett. 2007 Feb 06; 581(3):469-74. PMID: ... Differential modulation of 3T3-L1 adipogenesis mediated by 11beta-hydroxysteroid dehydrogenase-1 levels. J Biol Chem. 2007 Apr ...
The concept that disease rooted principally in chronic aberrant constitutive and reactive activation of mast cells (MCs), ... Prostaglandin D2 synthase enzymes and PPARγ are co-expressed in mouse 3T3-L1 adipocytes and human tissues. Prostaglandins Other ... the alpha chain of the interleukin-2 receptor, dominantly expressed by T-cells) and/or CD2 (ordinarily a surface adhesion ... AAAAI Mast Cell Disorders Committee Work Group report: mast cell activation syndrome (MCAS) diagnosis and management. J Allergy ...
The concept that disease rooted principally in chronic aberrant constitutive and reactive activation of mast cells (MCs), ... Prostaglandin D2 synthase enzymes and PPARγ are co-expressed in mouse 3T3-L1 adipocytes and human tissues. Prostaglandins Other ... the alpha chain of the interleukin-2 receptor, dominantly expressed by T-cells) and/or CD2 (ordinarily a surface adhesion ... AAAAI Mast Cell Disorders Committee Work Group report: mast cell activation syndrome (MCAS) diagnosis and management. J Allergy ...
3T3-L1 cells [91, 104]. Berberine can increase the ratio of AMP/ATP and activate AMPK and Akt pathways to promote acute insulin ... the G protein-coupled bile acid receptor TGR5 and repressing the S1P2/MAPK signaling pathway in glomerular mesangial cells. Exp ... Cellular stress responses: cell survival and cell death. Int J Cell Biol. 2010;2010(2010):214074. ... and magnoflorine can inhibit the lipid accumulation significantly in a dose-dependent manner in 3T3-L1 cells. They can down ...
... and NIH-3T3 cells (18.33% and 27.80%) and resulted in favouring cell-cycle arrest through increasing the accumulation of cells ... Further, ADP-1 beneficially affected lipid metabolism by enhancing lipid globule formation in mouse 3T3-L1 adipocytes. To our ... The receptor PPAR-alpha is predominantly expressed in adipose tissue. Ligand interactions with the receptor mediate specific ... In several adiponectin receptor-positive cancer cell lines, ADP 355 restricted proliferation in a dose-dependent manner at 100 ...
As a proof-ofconcept test, we applied cyclic stretch loading to the adipocyte model, differentiated adipocytes from 3T3-L1 ... Cells were differentiated on the stretchable membrane and then exposed to cyclic equibiaxial stretching using the Flexcell FX- ... 5000 device at a frequency of 1 Hz and a strain of 5% for 2 h. It was observed that expressions of insulin receptor substrate-1 ... we hypothesized that mechanical loading applied to the adipose tissue and its component cells, such as adipocytes, may play a ...
... and hepatic cells (9,10). Wang et al (11) have illustrated curcumins ability to reduce pro-inflammatory cytokines in 3T3-L1 ... L LiL ChenL HuNuclear factor high-mobility group box1 mediating the activation of Toll-like receptor 4 signaling in hepatocytes ... Two hundred random cells were counted for each condition. Cells were stimulated with HFFA in the absence (−) or presence (+) of ... Hepatocytes (1×103 cells) were cultured on glass coverslips. After incubation with HFFA and with or without curcumin, the cells ...