Random amplified polymorphic DNA technique: Definition with Random ...
Definition of Random amplified polymorphic DNA technique with photos and pictures, translations, sample usage, and additional links for more information.http://www.lexic.us/definition-of/random_amplified_polymorphic_dna_technique
Bioline International Official Site (site up-dated regularly)
NOTE: All figures may be accessed from references at the end of the text.]. Species-specific Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) markers were used to identify four species related to Anopheles (Nyssorhynchus) albitarsis Lynch-Arribalphalzaga from 12 sites in Brazil and 4 in Venezuela. In a previous study (Wilkerson et al. 1995), which included sites in Paraguay and Argentina, these four species were designated "A", "B", "C" and "D". It was hypothesized that species A is An. (Nys.) albitarsis, species B is undescribed, species C is An. (Nys.) marajoara Galvpio and Damasceno and species D is An. (Nys.) deaneorum Rosa-Freitas. Species D, previously characterized by RAPD-PCR from a small sample from northern Argentina and southern Brazil, is reported here from the type locality of An. (Nys.) deaneorum, Guajarbeta-Mirim, State of ...http://www.bioline.org.br/request?oc95147
Mycobacterium chimaera Outbreak Associated With Heater-Cooler Devices: Piecing the Puzzle Together
When 2 patients with disseminated M. chimaera infection following prosthetic valve surgery were detected at the University Hospital of Zurich in 2011, the implications of these findings were not immediately clear. 1 Mycobacterium chimaera is a slow-growing, nontuberculous mycobacterium (NTM) included in the M. avium complex (MAC). These opportunistic human pathogens are known to cause lung infections in those with underlying lung disease and disseminated infection in severely immunocompromised patients only. 2 Randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was performed to determine the clonal relationship of the 2 M. chimaera isolates. In contrast to the diversity seen among pulmonary M. chimaera isolates, these 2 isolates had identical RAPD-PCR patterns. 1 The hospital water system was investigated as a possible source, and M. chimaera was ...https://www.cambridge.org/core/journals/infection-control-and-hospital-epidemiology/article/mycobacterium-chimaera-outbreak-associated-with-heater-cooler-devices-piecing-the-puzzle-together/5E98B996F00A04AD46FFD74C8513B6D1/core-reader
Utility of random amplification of polymorphic DNA assay and B...
Utility of random amplification of polymorphic DNA assay and BOX-A PCR in molecular characterization of Streptococcus pneumoniae isolates recovered from varioushttps://www.mysciencework.com/publication/show/utility-random-amplification-polymorphic-dna-assay-box-pcr-molecular-characterization-streptococcus-pneumoniae-isolates-d99b2093
Intraspecific genetic diversity of Drechslera tritici-repentis as detected by random amplified polymorphic ...
The phytopathogenic fungus Drechslera tritici-repentis causes tan spot, an important disease of wheat in the southern Brazilian state of Rio Grande do Sul. Twelve D. tritici-repentis isolates were obtained from wheat seeds from different locations in the state. Their colony morphology on potato dextrose agar and polymorphisms in genomic DNA by the random amplified polymorphic DNA (RAPD) method were investigated. For the RAPD method, 23 primers were tested of which nine were selected for use in the study of D. tritici-repentis polymorphisms. The degree of similarity between isolates was calculated using a simple matching coefficient and dendrograms constructed by the unweighted pair-group method with arithmetical averages (UPGMA). The morphological and RAPD analyses showed intraspecific polymorphisms within the isolates, but ...http://www.lume.ufrgs.br/handle/10183/23347
Microbiology Society Journals | Genetic diversity within Lactobacillus sakei and Lactobacillus curvatus and design of PCR...
The genotypic and phenotypic diversity among isolates of the Lactobacillus curvatus/Lactobacillus graminis/Lactobacillus sakei group was evaluated by comparing RAPD data and results of biochemical tests, such as hydrolysis of arginine, D-lactate production, melibiose and xylose fermentation, and the presence of haem-dependent catalase. Analyses were applied to five type strains and to a collection of 165 isolates previously assigned to L. sakei or L. curvatus. Phenotypic and RAPD data were compared with each other and with previous DNA-DNA hybridization data. The phenotypic and genotypic separation between L. sakei, L. curvatus and L graminis was clear, and new insights into the detailed structure within L. sakei and L. curvatus were obtained. Individual strains could be typed by RAPD and, after the elimination of similar or identical isolates, two sub-groups in both L. curvatus and L. sakei were defined. The presence or absence of catalase ...http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-49-3-997
Analysis of genetic structure of Melia volkensii (Gurke.) populations using random amplified polymorphic DNA.
Melia volkensii (Gurke.) is a popular fast growing agroforestry tree species in the East Africa's arid and semi arid lands (ASALs). The species is valued for its high quality termite resistant timber. In addition, its fruits are eaten by livestock thus making it the species of choice by small-scale farmers. However, the species has been overexploited and information on its existing gene pool is currently lacking. The present work was therefore carried out using random amplified polymorphic DNA (RAPD) markers to assess genetic diversity within and between populations in order to suggest appropriate conservation and management strategies. Eight RAPD primers generated 38 scorable polymorphic bands which were used to estimate genetic distances between populations and for construction of neighbour-joining phenograms. Analysis of Molecular ...http://ir-library.ku.ac.ke/handle/123456789/7321
The utilization of micro-and macromolecular data for the analyses of evolutionary problems : examples from Juniperus and...
SUMMARY In the last decade, techniques based on DNA markers have been used to detect variation at DNA level and have proven to be reliable and very effective for distinguishing between closely related genotypes. The development of the random amplified polymorphic DNA (RAPD) markers (Williams et al., 1990; Welsh et al., 1991) allowed genome characterization in several plant groups. The RAPD technique has been useful in studying polymorphism, identifying genes of interest, and permitting the determination of phylogenetic relationships and intraspecific diversity at a molecular genetic level. In four minor studies, this thesis deals with the evolutionary relationships and genetic variation among different populations of plants in two genera of Juniperus and ...https://www.duo.uio.no/handle/10852/12094
Random Primer DNA Labeling Kit
The Random Primer DNA Labeling Kit, Version 2 is designed for radioactive labeling for hybridization probes. Probe labeling can label DNA with [32P]-alpha-, [35S]-alpha- or [3H]-alpha-dCTP. This random primer DNA labeling kit is based on a modified method by Feinberg and Vogelstein and utilizes random oligonucleotide primers and cloned exonuclease-free E.coli DNA polymerase I, Klenow fragment. Using primers that are 9-mer and longer and exonuclease-free enzyme results in higher labeling efficiency and longer probes. This probe labeling method overcomes many of the disadvantages of conventional nick translation procedures while producing hybridization probes from very small amounts of DNA (10 to 20 ng). The Radioactive Labeling for ...http://www.clontech.com/US/Products/Molecular_Biology_Tools/DNA_Labeling_Kits/Random_Primer_DNA_Labeling_Kit
Evaluation of Genetic Diversity among Soybean (Glycine max) Genotypes, Using ISJ and RAPD Molecular Markers
Abstract Soybean (Glycine max) is an important crop plant which contains a high amount of oil and protein. To evaluate the genetic diversity of 45 soybean genotypes growing in Iran, two types of molecular markers (RAPD and ISJ) were used. Two sets of different primers were used to compare potential differentiation power of IT (Intron targeting) and ET (Exon targeting) primers. Data obtained from each molecular marker was analyzed separately and in combination. Ten RAPD primers amplified 103 scoreable bands from which 60% were polymorphic, whereas 15 ISJ primes resulted to 129 sharp bands from which 87% were polymorphic. Cluster analysis was carried out based on simple matching coefficient of similarity and UPGMA method. The cophenetic coefficient for RAPD and ISJ markers were 0.95 and 0.81, respectively. Cluster analysis revealed that ISJ molecular markers were better in genetic diversity studies than RAPD ...http://breeding.tabrizu.ac.ir/article_3790.html
Multiple Alleles - Making Use Of Polymorphic Dna - Genetic, Genetic, and Gene - JRank Articles
Multiple alleles and noncoding polymorphic DNA are of considerable importance in gene mapping-identifying the relative positions of genetic loci on chromosomes. Gene maps are constructed by using the frequency of crossing-over to estimate the distance between a pair of loci. To obtain a good estimate, one must analyze a large number of offspring from a single cross. In laboratory organisms such as the fruit fly Drosophila, programmed crosses can be carried out so it is possible to use gene loci to construct a reliable genetic map. In humans, this is not the case. For this reason, the more highly variable noncoding regions are of considerable importance in human genetic mapping.. ...http://medicine.jrank.org/pages/2568/Multiple-Alleles-Making-Use-Polymorphic-DNA.html
Bioline International Official Site (site up-dated regularly)
and thus are genetically linked. The primers used to generate polymorphic bands were 3'-anchored inter-simple sequence repeat primers which identified genomic microsatellites with a repeated motif of 3 nucleotides in length. The primers were used singly to amplify genomic segments which were flanked by inversely orientated, closely spaced, identical microsatellite sequences. One of the polymorphic bands, a 900 base pair band, was completely linked to the Sr39 and Lr35 rust resistance genes in the segregating population used in this study. After cloning and sequencing this polymorphic band, the inter-simple sequence repeat marker was converted to a sequence characterized amplified region marker by designing primer sets which amplify a single, easily resolved band from DNA of plants with Sr39/Lr35 genes. This marker is present in six wheat ...http://www.bioline.org.br/abstract?id=ej99005&lang=en
Juniperus chinensis risk assessment
AB: Van Melle (1947) proposed that juniper cultivars of the Pfitzer Group were of hybrid origin and ascribed the name Juniperus x media Melle. This purported hybrid of J. chinensis x J. sabina has not been accepted unanimously by the horticultural community. Random amplified polymorphic DNAs (RAPDs) were used to analyse and establish new evidence for the hybrid origin of the Pfitzer Group, using both parents and seven cultivars of the Pfitzer Group. Principal coordinate analysis (PCO) of 122 RAPD bands demonstrated that samples of J. chinensis cluster tightly together, as do the J. sabina samples. Cultivars of the Pfitzer Group lacked affinity with either species, but stood apart as a distinct cluster. The data support Van Melle's conclusion that the Pfitzer Group is separate from J. chinensis and indicate hybrid origin from parents J. chinensis and J. sabina . Juniperus x ...http://www.hear.org/pier/wra/pacific/juniperus_chinensis_htmlwra.htm
RAPD method advice needed
I am just getting my hands wet with usenet so I hope I can be of some help although I am inexperienced in the usenet 'rules'. I'm not sure if you are requesting the actual precedues or just sources of literature, both of which I have. 'An alternative, rapid method of plant DNA extraction for PCR abalysis' by D. Brunel is in Nuc Acids Res vol.20, #17. There are other methods that I may be able to help you with, if the above reference is not what your looking for. -- Dr. Pepper 'He who controls the Spice, controls the universe!' Star Wars. Highlander. Seinfeld. WintermuteAI. YIJC. Moon Base Alpha. If Saddam, why not Milosevic? A nuclear free Earth, is where I want to live! The can be only one-Xavier! You Michigan folk, be nice losers this time ...http://www.bio.net/bionet/mm/plantbio/1993-March/001095.html
DNA Labeling Kit | Random Primer Labeling | Bca Polymerase
With this kit, random primer labeling via thermostable Bca polymerase is accomplished in 10 minutes, resulting in high yields of labeled DNA probe. The kit is designed for use with [32P]-alpha-, [35S]-alpha- or [3H]-alpha-dCTP labels, but it can also be used with nonradioactive biotin, digoxigenin, or DNP-labeled dUTPs through dNTP replacement. In general, probes with specific activities of ,109 dpm/mg DNA obtained with [32P]-alpha-dCTP (~3,000 Ci/mmol, 111 TBq/mmol).. ...http://www.clontech.com/US/Products/Molecular_Biology_Tools/DNA_Labeling_Kits/BcaBEST_DNA_Labeling_Kit?sitex=10020:22372:US&PEBCL1=IPwiIWVjaZbDgsjOGnqSa6Q7En&PEBCL1_pses=ZGD0C841FF463C3B06A164080927654D55F899C9FC37833A59758DBC4DB995F955CE98372B8B526686B340FC9E7FADB7B573D6A82857DD65A7
THE GENETIC VARIABILITY EVALUATED WITH MOLECULAR MARKERS ON THE BILBERRY TISSUE LINES
The tissue lines derived from the Arieșeni, Retezat and Valea Sebeșului bilberry genotypes grown on a WPM medium supplemented with 40, 60 and 80 mg/l AS, were subject to molecular analysis through amplification of DNA samples with ISSR and RAPD primers. The results of agarose gel electrophoresis of the amplification products show the existence of significant differences in the model of polymorphic bands obtained from tissue lines, compared to the mother plant. Based on the obtained results, the presence of somatic variability, induced in the tissue lines under the influence of AS, is emphasized. It consists in the absence of binding sites of the HB-12 ISSR marker compared to the mother plant. Considering the statements in the specialty literature, concerning the mutagenic effect of growth, we can state that AS caused changes within the DNA level in the bilberry calluses selected by us under the experimental ...http://biotechnologyjournal.usamv.ro/index.php/scientific-papers/165-the-genetic-variability-evaluated-with-molecular-markers-on-the-bilberry-tissue-lines
Sebeșului bilberry genotypes grown on a WPM medium supplemented with 40, 60 and 80 mg/l AS, were subject to molecular analysis through amplification of DNA samples with ISSR and RAPD primers. The results ...http://biotechnologyjournal.usamv.ro/index.php/component/search/?searchword=molecular%20analysis&areas%5B0%5D=tortags&Itemid=103
DNA stuck in wells
In article ,sfaruque-0701951437100001 at cfd-lab.umds.ac.uk,, sfaruque at uk.ac.mrc.hgmp (Mr SMNN Faruque) wrote: , Why, on occasions, does DNA seem to stick in wells? , , I've seen it before in horizontal gels, sequencing gels and now I've had , it in a vertical non-denaturing gel. , I've tried the 4% non-deturing gel (a band-shift assay) with TBE and TAE, , with Scholler buffer and BCA and the probe (144bp) doesn't seem to move , any distance into the gel even in the lane with just buffer and probe. , , Current Protocols... has no mention of this problem in its troubleshooting , section and I'm not sure what to try next. , , Thanks , Nadeem Faruque Dear Naadem, I performed bandshifts with 8% polyacrylamide gel for 4h at 250 V in 90 mM Tris-borate buffer, 10 mM EDTA pH 8.0. The probe was indeed smaller than yours, it was a 60mer with a 15mer primer annealed. The protein bound was 200 kD. Anyway, under the same conditions I succeded also in bandshifting a 400mer probe with ...http://www.bio.net/bionet/mm/methods/1995-January/022783.html
بررسی چند شکلی ژنتیکی در ارقام گلرنگ با استفاده از نشانگرRAPD
به منظور بررسی چند شکلی ژنتیکی بین ارقام داخلی و خارجی گلرنگ با استفاده از نشانگر RAPD تعداد 20 رقم گلرنگ مورد بررسی قرار گرفت. در این آزمایش از 17 آغازگر10 نوکلئوتیدی استفاده شد و در نهایت تعداد 279 نوار قابل امتیاز-دهی ایجاد شد که 256 نوار در محدوده-ای بین 100 و 3000 جفت باز را شامل می-شد. نمودارهای حاصل از روش UPGMA ارقام را به چهار گروه اصلی تقسیم کرد. گروه اول دو رقم خارجی، و گروه دوم 2 رقم بومی داخلی و یک رقم اصلاح شده را شامل می-شد. گروه سوم ارقام زراعی محلی، ارقام وحشی و اصلاح شده را شامل می-شد. گروه چهارم نیز شامل دو رقم اصلاح شده ایرانی اصفهان و رقم KW4 بود. بیشترینhttp://sustainagriculture.tabrizu.ac.ir/article_1095.html
DNA Analysis: Traditional Techniques - DNA Analysis: Traditional Techniques | HowStuffWorks
DNA Analysis: Traditional Techniques - Traditional DNA analysis techniques are explained in this section. Learn about traditional DNA analysis techniques.http://science.howstuffworks.com/life/genetic/dna-evidence2.htm
How do you create a DNA fingerprint? | Reference.com
Creating your own DNA fingerprint helps you to learn about DNA. This process takes about an hour to put together and overnight to set. You need a DNA sample, beakers, a laboratory, restriction...https://www.reference.com/science/create-dna-fingerprint-a25c78a231c39e74
Genetic similarities between European countries - Stormfront
Originally posted here: http://www.stormfront.org/forum/t873.../#post10427723 I wrote a script that used this data (Eupedia Y-DNA haplogroup frequencihttps://www.stormfront.org/forum/t900311/
Representative banding profiles associated with ERIC2 R | Open-i
Representative banding profiles associated with ERIC2 RAPD PCR in P. aeruginosa isolated from horses. Lane M: Molecular weight marker (100 bp); lane 1: genotypehttps://openi.nlm.nih.gov/detailedresult.php?img=PMC3113754_2046-0481-62-7-456-1&req=4
23 and Me Project
Advanced View: see yourself, your family, and your friends placed onto a map of genetic similarity, allowing the highest-resolution view into your ancestry ...http://www.jvfoa.com/Vick-and-Allied-Families-DNA-Project.php
Desvenlafaxine (Oral Route) Before Using - Mayo Clinic
Do not take desvenlafaxine with a monoamine oxidase (MAO) inhibitor (eg, isocarboxazid [Marplan®], linezolid (Zyvox®), methylene blue injection, phenelzine [Nardil®], selegiline [Eldepryl®], tranylcypromine [Parnate®]). Do not start taking desvenlafaxine during the 2 weeks after you stop a MAO inhibitor and wait 1 week after stopping desvenlafaxine before you start taking a MAO inhibitor. If you take them together or do not wait the proper amount of time, you may develop confusion, agitation, restlessness, stomach or intestinal symptoms, a sudden high body temperature, an extremely high blood pressure, or severe convulsions. Do not take any medicine that contains venlafaxine (Effexor®) while you are using Khedezla® or Pristiq®. Desvenlafaxine may cause some teenagers and young adults to be agitated, irritable, or display other abnormal behaviors. It may also cause some people to have suicidal thoughts and tendencies or to become more depressed. Some people may have trouble sleeping, get ...https://www.mayoclinic.org/drugs-supplements/desvenlafaxine-oral-route/before-using/drg-20071583?p=1