TatD is a central component of a Tat translocon‐initiated quality control system for exported FeS proteins in Escherichia coli ...
Casadaban MJ, Cohen SN (1979) Lactose genes fused to exogenous promoters in one step using a Mu‐lac bacteriophage: in vivo ... TatD is a central component of a Tat translocon‐initiated quality control system for exported FeS proteins in Escherichia coli ... independent protein translocation in Escherichia coli. A distinct and pivotal role for the TatB protein. J Biol Chem 274: 36073 ... Here, we identify TatD as a crucial component of this quality control system in Escherichia coli. NrfC/NapG variants lacking ...http://embor.embopress.org/content/10/5/474
RCSB PDB for 1BCO
Structure of the bacteriophage Mu transposase core: a common structural motif for DNA transposition and retroviral integration. ... BACTERIOPHAGE MU TRANSPOSASE A 327 Escherichia virus mu EC#: 3.1.22 IUBMB 6.5.1 IUBMB Gene Name(s): A Mup03 ... Organism: Escherichia virus Mu * Expression System: Escherichia coli Experimental Data Snapshot. *Method: X-RAY DIFFRACTION ... Toggle Protein Feature View. Show All Entities. Show First Few Entities. Molecule. Chains. Length. Organism. Details. ...http://www.rcsb.org/pdb/explore/biologyAndChemistry.do?structureId=1BCO
ASMscience | Site-specific DNA Inversion by Serine Recombinases
... particularly with regards to recent advances in our understanding of the function of the Fis/enhancer regulatory system. These ... on the extensively studied serine DNA invertases that are stringently controlled by the Fis-bound enhancer regulatory system. ... A dedicated recombinase, sometimes with the aid of additional regulatory or DNA architectural proteins, catalyzes the inversion ... Purification of the Gin recombination protein of Escherichia coli phage Mu and its host factor. Biochim Biophys Acta 866:170- ...http://www.asmscience.org/content/journal/microbiolspec/10.1128/microbiolspec.MDNA3-0047-2014
User:Aman Kumar - OpenWetWare
Bacteriophage Mu or phage Mu is a temperate bacteriophage that infects Escherichia coli and a wide range of other gram-negative ... system different from the one in which the phage had been last grown. Our objective is to purify the Mom protein and ... the mom gene. The gene mom stands for modification of mutator. This gene helps in modifying the DNA of the phage and acts as a ... Thus, mom gene acts as a protective sheath against nucleolytic attack when the Mu genome infects a bacterial cell possessing ...https://openwetware.org/wiki/?title=User:Aman_Kumar&oldid=801349
Frontiers | The C-terminal Six Amino Acids of the FNT Channel FocA Are Required for Formate Translocation But Not Homopentamer...
Removal of a further residue (L279) severely restricted formate export, the stability of the protein and its ability to form ... A Y-L-R motif is found immediately after transmembrane helix 6 at the C-terminus of FNT proteins related to FocA, or those with ... A Y-L-R motif is found immediately after transmembrane helix 6 at the C-terminus of FNT proteins related to FocA, or those with ... Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu. J. Mol. ...https://www.frontiersin.org/articles/10.3389/fmicb.2017.01616/full
Biochemistry of V(D)J Recombination | SpringerLink
The genes that encode immunoglobulin and T cell receptor proteins are assembled from component gene segments in a reaction ... ClpX protein of Escherichia coli activates bacteriophage Mu transposase in the strand transfer complex for initiation of Mu DNA ... The genes that encode immunoglobulin and T cell receptor proteins are assembled from component gene segments in a reaction ... Thompson CB (1995) New insights into V(D)J recombination and its role in the evolution of the immune system. Immunity 3:531-539 ...https://link.springer.com/chapter/10.1007/3-540-26363-2_4
Isolation and characterization of Ca2+-blind mutants of Yersinia pestis.
Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control ... Examination of the protein composition of the cell envelope of Escherichia coli by polyacrylamide gel electrophoresis. J ... A multipurpose cloning system. Gene. 1977;2(2):95-113. [PubMed]. *Bradford MM. A rapid and sensitive method for the ... Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene. 1979 May;6(1):23-28. [PubMed] ...http://pubmedcentralcanada.ca/pmcc/articles/PMC214309/?lang=en-ca
Ambivalent bacteriophages of different species active on Escherichia coli K12 and Salmonella sp. strains, Russian Journal of...
"Ambivalent bacteriophages of different species active on Escherichia coli K12 and Salmonella sp. strains, Russian Journal of ... Coliphage Which Requires Either the LamB Protein or the OmpC Protein for Adsorption to Escherichia coli K-12 ... System error. Please try again!. Thanks for helping us catch any problems with articles on DeepDyve. We'll do our best to fix ... Phage Mu. Koch, C.; Gabrielle, M.; Falko, R.. * Structure and Morphogenesis of Bacteriophage T4 ...https://www.deepdyve.com/lp/springer_journal/ambivalent-bacteriophages-of-different-species-active-on-escherichia-DBbzQl28SV
Proteins Involved in DNA Replication | Ulrich Huebscher | Springer
Autorepression of the dnaA Gene of Escherichia Coli. Atlung, Tove (et al.) ... Replication of Bacteriophage Mu and its Mini-Mu Derivatives. Resibois, Anne (et al.) ... Characterization of in Vitro DNA Synthesis in an Isolated Chloroplast System of Petunia Hybrida ... Gene A Protein of Bacteriophage ΦX174 is a Highly Specific Single-Strand Nuclease and Binds Via a Tyrosyl Residue to DNA After ...https://www.springer.com/us/book/9781468487329?wt_mc=
Bacteriophage P2 - Wikipedia
Odegrip, R., et al., The interaction of bacteriophage P2 B protein with Escherichia coli DnaB helicase. Journal of Virology, ... bacteriophage P2 applies a holin-endolysin system to lyse the host cell. P2 have two essential lysis genes (gene K and gene Y) ... Edlin, G., L. Lin, and R. Bitner, Reproductive fitness of P1, P2, and Mu lysogens of Escherichia coli. J Virol, 1977. 21(2): p ... Christie, G.E., et al., Regulation of bacteriophage P2 late-gene expression: the ogr gene. Proceedings of the National Academy ...https://en.wikipedia.org/wiki/Bacteriophage_P2
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Characteristics of a binding protein-dependent transport system for sn-glycerol 3-phosphate in Escherichia coli that is part of ... to the 5 or 3 end of a gene.. Use of bacteriophage transposon Mu d1 to determine the orientation for three proC-linked ... genes in Escherichia coli K-12. Why. (1997) Recognition oanda review forex peace army repair of compound DNA oanda review forex ... One global approach to analyzing protein- protein interactions reviwe to attach a nucleic-acid sequence oanda review forex ...http://retzepti.ru/oanda-review-forex-peace-army-1.html
CiteSeerX - Citation Query Bacteriophage P1 cloning system for the isolation, amplification and recovery of DNA fragments as...
Bacteriophage P1 cloning system for the isolation, amplification and recovery of DNA fragments as large as 100 kilobases pairs ... Escherichia coli, Shigella flexneri, Shigella dysenteriae, Klebsiella pneumoniae and Citrobacter freundii) and Pseudomonadaceae ... multi-gene loci, or multiple copies of two or more genes in fixed stoichiometry, plasmid or viral vectors may be inadequate. In ... protein production, transgenesis by Michael Lindenbaum, Erika Csonka, Elena Fleming, Lisa Garcia, Amy Greene, Lindsay Gung ...http://citeseerx.ist.psu.edu/showciting?cid=1091564
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... and MFS-Type Tripartite Multidrug Efflux Pumps of Escherichia coli. *11154918:Mutations in the waaR Gene of Escherichia coli ... 11154860:Dissection of the Bacteriophage Mu Strong Gyrase Site(SGS): Significance of the SGS Right Arm in Mu Biology and DNA ... 11154992:Glycosylation of the Self-Recognizing Escherichia coli Ag43 Autotransporter Protein. *11154993:Mechanism of Growth ... 11154889:Analysis of the Lateral Flagellar Gene System of Aeromonas hydrophila AH-3 ...http://www.100md.com/index/other/byinfono/022/310.htm
P1 phage - Wikipedia
The genome of P1 encodes 112 proteins and 5 untranslated genes and is this about twice the size of bacteriophage lambda. The ... P1 is a temperate bacteriophage that infects Escherichia coli and some other bacteria. When undergoing a lysogenic cycle the ... This system has close sequence homologies to recombinational systems in the tail fibers of unrelated phages like the mu phage ... Adams, David E.; James B. Bliska; Nicholas R. Cozzarelli (1992-08-05). "Cre-lox recombination in Escherichia coli cells ...https://en.wikipedia.org/wiki/P1_phage
Characterization of a chromosomally encoded, non-PTS metabolic pathway for sucrose utilization inEscherichia coliEC3132 |...
... mnemonic for chromosomally coded sucrose genes) transferred to strains of E. coli K12. ... that is able to grow on sucrose was isolated and its csc genes ( ... mini Mu derivative in Escherichia coli, Salmonella typhimurium ... and for a transport system CscB. Based on DNA sequencing studies, this last codes for a hydrophobic protein of 415 amino acids ... Rosner JL (1972) Formation, induction and curing of bacteriophage P1 lysogenes. Virology 49:679-689Google Scholar ...https://link.springer.com/article/10.1007%2FBF00286177
Bacterial Genetics Flashcards by John McClatchy | Brainscape
Consider conjugation in Escherichia coli. In which of the following matings would chromosomal genes be transferred most ... A: is a lysogenic bacteriophage.. B: cannot infect new host cells.. C: has a viral coat made of host proteins.. D: contains ... A transduction by a dsDNA phage Mu. B conjugation of a linear piece of DNA. C transformation of a linear piece of DNA. D gene ... What characteristic of the S strain allows it to evade the immune system of the mice?. A The cells have a capsule.. B They are ...https://www.brainscape.com/flashcards/bacterial-genetics-5623466/packs/8402273
Zymographic differentiation of [NiFe]-Hydrogenases 1, 2 and 3 of Escherichia coli K-12
Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu. J Mol ... μg of protein) to the gels. Alternatively, for neutral pH analyses the barbitone gel system was used. This system uses final ... Sec-independent protein translocation in Escherichia coli. A distinct and pivotal role for the TatB protein. J Biol Chem. 1999; ... A-type carrier protein ErpA is essential for formation of an active formate-nitrate respiratory pathway in Escherichia coli K- ...http://pubmedcentralcanada.ca/pmcc/articles/PMC3431244/
Frontiers | Commentary: Programmable base editing of A·T to G·C in genomic DNA without DNA cleavage | Genetics
... gene function, and protein networks (Doudna and Charpentier, 2014; Zhang et al., 2014; Amitai and Sorek, 2016). This function ... exploits a modified version of the CRISPR-Cas9 system and an RNA-based deamination enzyme.INTRODUCTION: GENOME-EDITING ... a microbial cellular immunity system (Barrangou et al., 2007), allows the precise editing of DNA sequences and interrogation of ... requires the presence of a set of CRISPR-associated (Cas) genes, which usually are found adjacent to the CRISPR locus. The wild ...https://www.frontiersin.org/articles/10.3389/fgene.2018.00021/full
ASMscience | Prophage Arsenal of Salm
... which is encoded by a chromosomal gene. A disruption of the grvA locus was shown to render S. enterica serovar Typhimurium more ... Early studies also indicated that some genes of certain prophages escape lysogenic repression and express functions that modify ... DNA inversions in the chromosome of Escherichia coli and in bacteriophage Mu: relationship to other site-specific recombination ... 1997.The invasion- associated type-III protein secretion system in Salmonella. Gene 192:51-59.. ...http://www.asmscience.org/content/book/10.1128/9781555816506.chap8
Nomenclature | Journal of Bacteriology
... shows that a hybrid protein is synthesized. An inversion is shown as IN(rrnD-rrnE)1. An insertion of an E. coli his gene into ... Binary names, consisting of a generic name and a specific epithet (e.g., Escherichia coli), must be used for all microorganisms ... ΔMu cts, or mal::ΔMu cts::lac. In setting parenthetical remarks within the genotype or dividing the genotype into constituent ... The system of designating transposon insertions at sites where there are no known loci, e.g., zef-123::Tn5, has been described ...https://jb.asm.org/content/nomenclature
Bacteriophage P1 Ban protein is a hexameric DNA helicase that interacts with and substitutes for Escherichia coli DnaB. -...
We isolated and sequenced the ban gene, purified the product, and analysed the function of Ban protein in vitro and in vivo. ... Since all existing conditionally lethal dnaB strains bear DnaB proteins that may interfere with the protein under study, we ... a dnaB null strain by using a genetic set-up designed to provoke the conditional loss of the entire dnaB gene from E.coli cells ... We discuss the interaction of bacterial proteins and their phage-encoded analogues to fulfil functions that are essential to ...https://www.semanticscholar.org/paper/Bacteriophage-P1-Ban-protein-is-a-hexameric-DNA-and-Lemonnier-Ziegelin/be614bb98d3f5eb0fd9ad6a8b45caadce0b54628
Bacteriophage therapy to combat bacterial infections in poultry | Virology Journal | Full Text
1). Examples of bacteriophages with a lysogenic cycle include λ Escherichia coli; Mu, with activity against E. coli, Salmonella ... The murein hydrolase of the bacteriophage phi3626 dual lysis system is active against all tested Clostridium perfringens ... Only full characterization and screening of phages can eliminate those that encode toxic proteins or proteins that allow ... They can also be involved in transferring drug-resistance genes among bacteria. Another adverse phenomenon in phage therapy is ...https://virologyj.biomedcentral.com/articles/10.1186/s12985-017-0849-7
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Transposition and fusion of the lac genes to selected optiтns in Escherichia coli using bacteriophage lambda and Mu. Campbell, ... The pho regulon-dependent Binary options indicator software uptake system for glycerol-3-phosphate in Escherichia coli is trans ... This provides evidence that sbcB point mutant alleles do in fact produce a protein that contributes to the cell phenotype (13). ... coli. 59) and Gˆ in (9. Beer (eds. Coli alkaline phosphatase gene, was subcloned into the pTZ18U plasmid (Pharmacia). 543 4. ...http://imbc-med.ru/binary-options-indicator-software-6.html
ASMscience | Iteron Plasmids
... also discuss the current understanding of control mechanisms affecting the plasmid copy number and how host chaperone proteins ... the structure of the iteron plasmid encoded replication initiation proteins, and the molecular mechanisms for iteron plasmid ... ClpX protein of Escherichia coli activates bacteriophage Mu transposase in the strand transfer complex for initiation of Mu DNA ... encoded by the repA gene of phage P1 and the dnaA gene of Escherichia coli. Proc Natl Acad Sci USA 84:3668-3672. [PubMed][ ...http://www.asmscience.org/content/journal/microbiolspec/10.1128/microbiolspec.PLAS-0026-2014
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Lac- tose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage in vivo probe for transcription binary ... In vitro methylation of Escherichia coli 16S rRNA by tRNA(mU54)-methyltransferase. ... Methods 3. This protein complex, on the surface of resin sections, can afterwards be amplified by heavy metal stain (13) as ... Sugar transport by the bacterial phosphotransferase system.. 40 OPTICAL AND PHYSICAL PROPERTIES FIGURE 29 1992. McGrath, P. ...http://imbc-med.ru/binary-option-trading-free-account-1.html