*  New Yeast Cloning System for Producing Proteins with Native Amino Acid,,,Sequences ( One-column protein purification and n...)
Yeast,Cloning,System,for,Producing,Proteins,with,Native,Amino,Acid,,,Sequences,biological,advanced biology technology,biology ... One-column protein purification and native eukaryotic amino acid se... figure 1 ...Stratagenes ESP LIC cloning and expression ... Electroporation of Yeast Artificial Chromosomes. 4. New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting. 5 ... Mammalian Expression Vector for Efficient Cloning of PCR Fragments. 10. QIAGEN PCR CloningPlus Kit. 11. Cloning Based on ...
  http://www.bio-medicine.org/biology-technology/New-Yeast-Cloning-System-for-Producing-Proteins-with-Native-Amino-Acid-0D-0A-0ASequences-44-1/
*  CiteSeerX - Citation Query Bacteriophage P1 cloning system for the isolation, amplification and recovery of DNA fragments as...
... amplification and recovery of DNA fragments as large as 100 kilobases pairs ... with carrying capacities for yeast artificial chromosomes in excess of 1 Mb (5). However, an additional limitation that the ... A mammalian artificial chromosome engineering system (ACE System) applicable to biopharmaceutical ...". Abstract - Add to ... protein production, transgenesis by Michael Lindenbaum, Erika Csonka, Elena Fleming, Lisa Garcia, Amy Greene, Lindsay Gung ...
  http://citeseerx.ist.psu.edu/showciting?cid=1091564
*  Pulsed Field Gel Electrophoresis - A. P. Monaco - Oxford University Press
... has provided a very reliable system for separation of DNA fragments greater than 50 kb and has made a significant impact on the ... PFGE analysis of yeast artificial chromosomes. 7. Functional analysis of mammalian genomes using yeast artificial chromosomes. ... 4. Cloning from PFGE-purified genomic DNA fragments and YACs. 5. Yeast artificial chromosome cloning using PFGE. 6. ... and transfer of yeast artificial chromosomes (YACs). The final two chapters describe the use of PFGE in the analysis of ...
  https://global.oup.com/academic/product/pulsed-field-gel-electrophoresis-9780199635351?cc=us&lang=en&tab=description
*  clone facts, information, pictures | Encyclopedia.com articles about clone
Bacterial artificial chromosomes (BACs) can contain up to 300 kb, and yeast artificial chromosomes (YACs), grown in yeast cells ... Cloning into the bacterial virus bacteriophage λ allows use of fragments up to about 20 kb. In this scheme, the bacteriophage ... In a true mammalian clone (as in Gurdon's frog clone) the nucleus from a body cell of an animal is inserted into an egg, which ... replicate (a fragment of DNA placed in an organism) so that there is enough to analyze or use in protein production. ∎ ...
  https://www.encyclopedia.com/science-and-technology/biology-and-genetics/biology-general/clone
*  US20080108513A1 - Cellular Arrays - Google Patents
... "a portion of a given protein") refers to fragments of that protein. The fragments may range in size from four amino acid ... Cloning and expression vectors for use with bacterial, fungal, yeast, and mammalian cellular hosts are known in the art, and ... The resulting DNA then stably integrates into cellular chromosomes as a provirus and directs synthesis of viral proteins. The ... refers to an artificial environment and to processes or reactions that occur within an artificial environment. In vitro ...
  https://patents.google.com/patent/US20080108513A1/en
*  Indian Patents. 233626:'A PURIFIED GLYCOPROTEIN'
Also contemplated are vectors that are artificial chromosomes, such as yeast artificial chromosomes and mammalian artificial ... 0309] For example, N-and C-terminal fragments of the sHASEGP can be expressed in bacteria and used to search for proteins that ... Lysate proteins binding to the fusion proteins can be resolved by SDS-PAGE, isolated and identified by protein sequencing or ... The yeast two-hybrid system has been used to identify other protein partner pairs and can readily be adapted to employ the ...
  http://www.allindianpatents.com/patents/233626-a-purified-glycoprotein
*  Generation of xenogeneic antibodies - Abqenix, Inc.
The subject invention provides non-human mammalian hosts characterized by inactivated endogenous Ig loci and functional human ... describe the construction of a library of yeast artificial chromosomes containing human DNA fragments. Yeast artificial ... 0003] The field of this invention is the production of xenogeneic specific binding proteins in a viable mammalian host. ... Cloning of Human Heavy Chain Locus using Yeast Artificial Chromosomes [0172] A. Production of Yeast Artificial Chromosome (YAC ...
  http://www.freepatentsonline.com/y2003/0229905.html
*  Human antibodies derived from immunized xenomice - Abgenix, Inc.
Garza, et al., "Mapping the drosophila genome with yeast artificial chromosomes with yeast artificial chromosomes", Science 246 ... 9 shows a diagram of a plasmid used to transfect mammalian cells to effect the production of the human protein gp39. ... Such fragments are sufficient for use, for example, in immunodiagnostic procedures involving coupling the immunospecific ... Gnirke, et al., "Cloning and in vivo expression of the human GART gene using yeast artificial chromosomes", EMBO Journal 10(7): ...
  http://www.freepatentsonline.com/6150584.html
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... artificial chromosomes, RNA, proteins and small molecules into mice, rats and, to a limited degree, other animals. This ... Genetic Studies of Human DNA Repair Proteins Using Yeast as a Model System ... a protein of the RNase III family of endonucleases. The resulting fragments are components of the RNA-induced silencing complex ... Once infected mammalian host, T. brucei cell regularly switches its surface antigen to evade the host's immune attack. We have ...
  https://www.jove.com/visualize/abstract/24223931/identification-wee1-like-kinase-gene-essential-for-procyclic
*  Frontiers | Progress and Challenges in the Design and Clinical Development of Antibodies for Cancer Therapy | Immunology
... of B cell development by variable gene complexity in mice reconstituted with human immunoglobulin yeast artificial chromosomes ... Applications of yeast surface display for protein engineering. Methods Mol Biol (2015) 1319:155-75. doi:10.1007/978-1-4939-2748 ... and mammalian (107) display. Each of these platforms has advantages and disadvantages. One of the advantages of yeast display, ... while phage is limited to the display of antibody fragments such as scFvs or Fabs, yeast enables the display of full IgG ...
  https://www.frontiersin.org/articles/10.3389/fimmu.2017.01751/full
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Here we discuss critical factors for designing a yeast system for the expression of bacterial type III effector proteins. These ... Cells that use these substrates to repair broken chromosomes by HR form an intact HIS3 allele and a tXV::III chromosomal ... Natural competence allows the uptake of free DNA including PCR-generated DNA fragments. Once taken up, the DNA recombines with ... or artificial transformation of the latter; and the excision of the plasmid is random and can either restore the initial ...
  https://www.jove.com/visualize/abstract/23646187/multiple-pathways-suppress-non-allelic-homologous-recombination
*  CLONAL EXPANSION OF B CELLS - Patent application
... or artificial chromosome vectors such as bacterial artificial chromosomes (BAC), yeast artificial chromosomes (YAC), or P1 ... Recombinant Proteins of the Present Invention [0176] To reproduce the CD40 signal we have generated novel fusion proteins, ... Mammalian cells are particularly preferred, such as cells from humans, mice, hamsters, pigs, goats, and primates. The cells may ... fragments. [0098] The term "host cell" relates according to the invention to any cell which can be transformed or transfected ...
  http://www.patentsencyclopedia.com/app/20120219991
*  Patent US7078492 - Human antipneumococcal antibodies from non-human animals - Google Patents
7:13-21 (1994). The XenoMouse™ strains were engineered with yeast artificial chromosomes (YACs) containing 245 kb- and 190 kb- ... Preferred regulatory sequences for mammalian host cell expression include viral elements that direct high levels of protein ... Fragments typically are at least 5, 6, 8 or 10 amino acids long, preferably at least 14 amino acids long, more preferably at ... protein glycosylation, protein cleavage, protein secretion, intracellular protein localization and extracellular protein homing ...
  http://www.google.com/patents/US7078492?dq=5910382
*  Difference between revisions of "User:Jarle Pahr/Articles" - OpenWetWare
Transferring a synthetic gene circuit from yeast to mammalian cells: http://www.nature.com/ncomms/journal/v4/n2/full/ncomms2471 ... Precise Manipulation of Chromosomes in Vivo Enables Genome-Wide Codon Replacement 2012. A novel PCR-based method for high ... DNA Fragments Assembly Based on Nicking Enzyme System: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3590185/ ... Artificial repressors for controlling gene expression in bacteria: http://pubs.rsc.org/en/content/articlepdf/2013/cc/c2cc37107c ...
  https://openwetware.org/wiki/?title=User:Jarle_Pahr/Articles&curid=130354&diff=685253&oldid=684404
*  PLANT CENTROMERE COMPOSITIONS - Patent application
... maintained as artificial chromosomes. These yeast artificial chromosomes (YACs) contain cloned genes, origins of replication, ... Alternatively, the protein may be a storage protein, such as a zein, or a structural protein, the decreased expression of which ... broken chromosomes that lack a centromere (acentric chromosomes) are rapidly lost from cell lines, while fragments that have a ... 0580] Willard, H., Nature Genetics 15:345-354, 1997 [0581] Willard, H., "Centromeres of mammalian chromosomes" Trends Genet., 6 ...
  http://www.patentsencyclopedia.com/app/20110189774
*  Gene Pulser Xcell™ Electroporation Systems | Life Science Research | Bio-Rad
Preset transfection protocols for common mammalian and bacterial cells; easy programming. ... Electroporation of Yeast Artificial Chromosomes. Click to download 1358. Efficient Cloning and Electro-transformation of Large ... Publication List: Electroporation of Nucleic Acids and Proteins for CRISPR-Related Gene Editing, Ver A. Click to download ... Preset protocols - include the most common mammalian and bacterial cell types. *Flexibility - choice of programs for preset ...
  http://www.bio-rad.com/en-us/product/gene-pulser-xcell-electroporation-systems?pcp_loc=lnav
*  Books - Lane Medical Library - Stanford University School of Medicine
The use of bacterial artificial chromosomes for recombinant protein production in mammalian cell lines / Leander Blaas [and ... Optimising yeast as a host for recombinant protein production (review) / Nicklas Bonander and Roslyn M. Bill -- Which yeast ... Production of antibody fragments in Arabidopsis seeds / Bart Van Droogenbroeck, Kirsten De Wilde, and Ann Depicker -- ... Regulated proteolysis in yeast -- Roles of Cdc48 in regulated protein degradation in yeast / Alexander Buchberger -- The Role ...
  http://lane.stanford.edu/biomed-resources/eb.html?a=r&page=2&proxy-links=false
*  Artificial chromosome - Patent # 6716608 - PatentGenius
... and further preparing a yeast strain in which mammalian telomere sequences are added to the ends of its chromosome. ... preparing a CEPH artificial yeast chromosome library containing a human genome, identifying clones having a repetitive human ... mammalian centromere sequence and a DNA replication origin with mammalian telomere sequences added to both ends of the clone, ... An artificial mammalian chromosome, more specifically, a clone containing a ...
  http://www.patentgenius.com/patent/6716608.html
*  Patent US5858777 - Methods and reagents for regulating telomere length and telomerase activity - Google Patents
... to those proteins or fragments thereof can be used to detect regulators of telomere length and telomerase activity in mammalian ... Purified and recombinant proteins TPC2 and TPC3 and recombinant or synthetic oligonucleotides corresponding ... in yeast artificial chromosomes, cosmids, or bacteriophage lambda (e.g., Charon 35), with a polynucleotide probe comprising a ... These rare proteins and the mRNAs that encode these proteins are present in very low abundance in mammalian cells, ...
  http://www.google.com/patents/US5858777?dq=5,815,488
*  Patent US7981863 - Treatment of Parkinson's disease with PDGF - Google Patents
The human genes are incorporated, for example, using yeast artificial chromosomes containing the requisite human DNA segments. ... fragments, analogs or homologs thereof. Antibody fragments that contain the idiotypes to a protein antigen may be produced by ... a nucleic acid encoding a reagent is expressed in mammalian cells using a mammalian expression vector. Examples of mammalian ... The full-length protein can be used or, alternatively, the invention provides antigenic peptide fragments of the antigen for ...
  http://www.google.com/patents/US7981863?dq=7,134,016
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... artificial chromosomes, RNA, proteins and small molecules into mice, rats and, to a limited degree, other animals. This ... Mammalian Toll-like receptors (TLRs) are a family of receptors that recognize pathogen-associated molecular patterns. Not only ... The vessel fragments underwent a second enzymatic digestion to free endothelial cells from their extracellular matrix. The ... Information gained from this yeast model is relevant to many organisms, including humans, since DNA repair and DNA damage ...
  https://www.jove.com/visualize/abstract/20976108/aav-mediated-gene-delivery-adult-gm1-gangliosidosis-mice-corrects
*  Patent US7208300 - Member of the lysyl oxidase gene family - Google Patents
The invention further relates to an assay system to identify compounds that selectively modulate EER-7 protein activity by ... The invention relates to the protein and nucleic acids encoding the protein. ... Isolated nucleic acid molecules include sequences inserted into plasmids, cosmids, artificial chromosomes, and the like. Thus, ... Alternatively, cells (including without limitation mammalian, insect, yeast, or bacterial cells) are transiently programmed to ...
  http://www.google.com/patents/US7208300?dq=5787449
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Cellular Biology, Issue 36, protein-protein interaction, membrane, split-ubiquitin, yeast, library screening, Y2H, yeast two- ... of protein-protein interactions. Briefly, the integral membrane bait protein is fused to Cub which is linked to an artificial ... To test for an interaction between two proteins of interest in cultured mammalian cells, one protein is expressed as a fusion ... is used against specific protein factors to enrich chromatin fragments of interest before proximity ligation. Ligation ...
  https://www.jove.com/visualize/abstract/22952839/swi1-associates-with-chromatin-through-ddt-domain-recruits-swi3-to
*  Patent US8088908 - Humanized anti-prostate stem cell antigen monoclonal antibody - Google Patents
... artificial chromosomes such as yeast artificial chromosome (YAC), bacterial artificial chromosome (BAC), or P1-derived ... Characterization of engineered anti-p185HER-2 (scFv-CH3)2 antibody fragments (minibodies) for tumor targeting. Protein Eng Des ... Mammalian expression and hollow fiber bioreactor production of recombinant anti-CEA diabody and minibody for clinical ... Characterization of engineered anti-p185HER-2 (scFv-CH3)2 antibody fragments (minibodies) for tumor targeting. Protein Eng Des ...
  http://www.google.co.uk/patents/US8088908
*  Patente US6025194 - Nucleic acid sequence of senescence asssociated gene - Google Patentes
... as well as peptides and antibodies corresponding to the GC6 protein can be used to identify senescent cells, distinguish ... Isolated, purified, and recombinant nucleic acids and proteins corresponding to the human GC6 gene and its mRNA and protein ... or artificial chromosome constructs such as yeast artificial chromosomes, bacterial artificial chromosomes, bacterial P1 ... In mammalian cell systems, promoters from the mammalian genes or from mammalian viruses are most appropriate. If it is ...
  http://www.google.es/patents/US6025194