We have previously reported the identification and characterization of an LKB1-specific adaptor protein, STRAD, whic … ... Adaptor Proteins, Vesicular Transport / genetics * Adaptor Proteins, Vesicular Transport / metabolism* * Caco-2 Cells ... The junctional proteins ZO-1 and p120 redistribute in a dotted circle peripheral to the brush border, in the absence of cell- ... Complete polarization of single intestinal epithelial cells upon activation of LKB1 by STRAD Cell. 2004 Feb 6;116(3):457-66. ...

Carrier Proteins / metabolism* * Carrier Proteins / pharmacology * Child * Colon / metabolism * Colonic Neoplasms / blood ... Caco-2 Cells * Carrier Proteins / blood * ...

Mesoporous silica nanoparticles for intracellular delivery of membrane impermeable proteins. J Am Chem Soc 129:8845-8849 ... The cell viability of Caco-2 cells was affected during MSNs-GA uptake. MSNs could be designed as suitable nanocarriers for ... Highly efficient cellular labeling of mesoporous nanoparticles in human mesenchymal stem cells: implication for stem cell ... For MSNs-GA, cell viability was reduced as a function of incubation time and different concentrations of nanoparticles. The in ...

October 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. ... which transcytoses bidirectionally in Caco-2 cells". The Journal of Biological Chemistry. 270 (35): 20717-20723. doi:10.1074/ ... 2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3 (1): 89. ... "Vaccinia virus intermediate stage transcription is complemented by Ras-GTPase-activating protein SH3 domain-binding protein ( ...

Title: HISTIDINE CONTENT OF LOW MOLECULAR WEIGHT BEEF PROTEINS INFLUENCES NONHEME IRON BIOAVAILABILITY IN CACO-2 CELLS Author. ... HISTIDINE CONTENT OF LOW MOLECULAR WEIGHT BEEF PROTEINS INFLUENCES NONHEME IRON BIOAVAILABILITY IN CACO-2 CELLS. JOURNAL OF ... Technical Abstract: The objective of this study was to isolate and characterize beef muscle proteins that enhance nonheme iron ... Egg, cheese, milk, and ovalbumin proteins do not. The research described in this work has identified meat factors that may play ...

... cells, in a dose-dependent manner. Adenine induced significant apoptosis in HT29 cells, whereas Caco-2 cells ... i,Methods,/i,. Cell viability was measured using the MTT assay. Levels of phosphorylation and protein expression were ... The data showed that adenine activated AMP-activated protein kinase (AMPK) signaling contributing to autophagic cell death ... i,Results,/i,. Adenine significantly inhibited the viability of colon cancer cells, HT29 and Caco-2  ...

... protein disassembly and increased intestinal epithelial barrier (IEB) permeability. Our study used Caco-2 cells as an in vitro ... cell-cell [...] Read more. Cell to cell communication is essential for the coordination and proper organization of different ... The unfolded protein response (UPR) is a cell-signaling system that detects the accumulation of unfolded protein within the ... The unfolded protein response (UPR) is a cell-signaling system that detects the accumulation of unfolded protein within the ...

The (13)C-isotopologue patterns of protein-derived amino acids from the bacteria and the host cells were then determined by ... Surprisingly, the metabolism of the host cells, as judged by the efficiency of (13)C-incorporation into host cell amino acids, ... replicating in epithelial colorectal adenocarcinoma cells (Caco-2). To this aim, we supplied [U-(13)C(6)]glucose to Caco-2 ... The data showed that EIEC HN280 growing in the cytosol of the host cells, as well as Stm 14028 replicating in the Salmonella- ...

... cell line to the burger samples.. In addition, the protein content in the Caco-2 cell lysate was determined using bovine serum ... Tags: Adenocarcinoma, Albumin, Anemia, Bovine Serum Albumin, Calcium, Cell, Cell Line, Colorectal, Copper, Diabetes, Diet, ... Protein, Pumpkin, Spectrometry, Type 2 Diabetes, Vegan, Vegetarian, Vitamin B12, Vitamin C, Vitamin D, Wheat, Zinc ... whereas burgers comprising soy protein comprised four-fold more iron (15 mg of iron per 100 g) than beef burgers. However, iron ...

The two strains mediate adhesion by different mechanisms and, when combined, synergically induce the expression of Caco-2 tight ... junction proteins. Moreover, growth inhibition experiments demonstrated the ability of the two strains alone and in combination ... The two strains mediate adhesion by different mechanisms and, when combined, synergically induce the expression of Caco-2 tight ... junction proteins. Moreover, growth inhibition experiments demonstrated the ability of the two strains alone and in combination ...

Production of protein extracts from Swedish red, green, and brown seaweeds, Porphyra umbilicalis Kutzing, Ulva lactuca Linnaeus ... In vitro digestibility and Caco-2 cell bioavailability of sea lettuce (Ulva fenestrata) proteins extracted with pH-shift ... Cultivation of Ulva fenestrata using herring production process waters increases biomass yield and protein content ... Strategies for Improving the Protein Yield in pH-Shift Processing of Ulva lactuca Linnaeus: Effects of Ulvan Lyases, pH- ...

ERN2 acted by splicing Xbp1 mRNA to expand ER function and prevent ER stress in goblet cells. Although ERN1 can also splice ... Myosin VB was identified by mass spectrometry as client of the UNC45A chaperone and was found misfolded in UNC45AKO Caco-2 ... Enhanced de novo lipogenesis mediated by sterol regulatory element-binding proteins (SREBPs) is thought to be involved in ... cells. In keeping with impaired myosin VB function, UNC45AKO Caco-2 cells showed abnormal epithelial morphogenesis that was ...

Topology analysis identified a set of proteins directly linked to the SAR-CoV2 titer, including ACE2, a SARS-CoV-2 binding ... Taken together, the data output from this network has identified nodal host proteins that may be connected to 18 chemical ... The current study employed Bayesian networks to a longitudinal proteomic dataset generated from Caco-2 cells transfected with ... SARS-CoV-2 (isolated from patients returning from Wuhan to Frankfurt). Two different approaches were employed to assess the ...

We used a cell-based human FXR β-lactamase (Bla) reporter gene assay to profile the Tox21 10K compound collection of ... Campana, G., Pasini, P., Roda, A. & Spampinato, S. Regulation of ileal bile acid-binding protein expression in Caco-2 cells by ... Cell culture. The cell line and the cell culture reagents were purchased from the Life Technologies (Carlsbad, CA, USA). The ... bla cells at a cell density of 1000 cells/μL was plated in 1536-well tissue culture-treated black clear bottom plates (Greiner ...

In various cell types [examples are (primary) human endothelial cell lines, human colon Caco-2, or HCT-8 epithelial cells ( ... However, if the glycosphingolipid still needs to be capable of binding a specific protein, an exact knowledge of the protein in ... is protein free. However, the mode of protein binding appears to be different. Given by the observed height differences in the ... results in the formation of high-density protein clusters and low-density protein areas. Such clusters in combination with Gb3 ...

Methods to Study Epithelial Transport Protein Function and Expression in Native Intestine and Caco-2 Cells Grown in 3D… ... Photobleaching Assays (FRAP & FLIP) to Measure Chromatin Protein Dynamics in Living Embryonic Stem Cells ... Methods to Study Epithelial Transport Protein Function and Expression in Native Intestine and Caco-2 Cells Grown in 3D… ... In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability… ...

... cell proliferation; and protective functions by inhibiting endogenous proteases. Finally, SpaFED pili are clearly important ... Our results show that CNCM I-3690 can (i) physically maintain modulated goblet cells and the mucus layer and (ii) counteract ... 11 fibronectin-binding proteins (FBPs), 10 choline-binding proteins (CBPs), and 28 mucus-binding proteins (MBPs) (Fig. 1). They ... The human cell lines Caco-2 (ATCC, UK), NF-κβ/SEAPorter HEK 293 (Imgenex, France), and HT-29 (ATCC) were grown in Dulbeccos ...

Cell Culture. The Caco-2 cells, a human colon cancer line, were obtained from Dr. Jing Yu, (Tuffs School of medicine; Medford, ... The protein content was determined through the use of Bradford (1976) method. The same method was applied to Caco-2 cells ... Cells were then re-incubated for an additional 4 h at 37°C. Thereafter, the cells were carefully washed twice with 200 μL of ... Cells were then incubated for 72 h. In this test, a control group (DMEM without extract) and a blank group (without cells or ...

Western blotting was performed to determine the protein level of EGFR in Caco-2 cells. Compared with the control group ... In vitro cell line experiments. Cell culture and transfection. The human colon cancer cell line Caco-2 was obtained from the ... Figure 1: Reduced the proliferation rate and migration ability of human cancer cells (Caco-2) caused by EGFR knockdown. (A) ... In vitro cell line experiments. Knockdown of EGFR expression in Caco-2 cells. ...

New research can confirm that while some of the response from monocytes to SARS-CoV-2 is generic to virus infection, most of it ... Human colorectal adenocarcinoma cell line Caco-2, known to be susceptible to SARS-CoV-2 infection, was used in place of primary ... To investigate which viral proteins could be involved in the SARS-CoV-2 specific response, Caco-2 cells were transfected with a ... Tags: Adenocarcinoma, Anosmia, B Cell, Blood, CCL3, CCL4, Cell, Cell Line, Chemokines, Colorectal, Coronavirus, Coronavirus ...

Dev Cell 34, 19-32.. The Goblet Cell Protein Clca1 (Alias mClca3 or Gob-5) Is Not Required for Intestinal Mucus Synthesis, ... "Glycocholic acid and butyrate synergistically increase vitamin D-induced calcium uptake in Caco-2 intestinal epithelial cell ... Cell Host Microbe. 2015 Nov 11;18(5):582-92.. Expression, sorting, and segregation of Golgi proteins during germ cell ... Identification of stem cell factors. Novel protein-protein interactions and their functions. 2010. Supervisor: Stina Simonsson ...

Silencing the Breast Cancer Resistance Protein Expression and Function in Caco-2 Cells Using Lentiviral Vector-Based Short ... Multidrug Resistance Protein 2-Mediated Estradiol-17β-d-glucuronide Transport Potentiation: In Vitro-in Vivo Correlation and ... Dual Mechanisms of CYP3A Protein Regulation by Proinflammatory Cytokine Stimulation in Primary Hepatocyte Cultures Choon-Myung ... Saturation of Multidrug-Resistant Protein 2 (Mrp2/Abcc2)-Mediated Hepatobiliary Secretion: Nonlinear Pharmacokinetics of a ...

... protein bound to both MA104 cells and murine enterocytes. However, further analysis of this protein by immunoprecipitation, ... we utilized a cell culture-adapted rhesus rotavirus (RRV) to infect human intestinal (Caco-2) and Madin-Darby canine kidney ( ... L cells and HEp2 cells were relatively resistant to rotavirus infection compared with permissive Ma104 cells and HT29 cells. ... L cells and HEp2 cells were as permissive as Ma104 and HT29 cells when rotavirus infection was mediated by transfection of ...

Our work so far has proved the beneficial energy and protein profiles of X. caffra (7), M. zeyheri (8) and K. africana (9) ... a commonly used gastrointestinal cell line, and human embryonic kidney (HEK-293), an epithelial immortalised cell line. Caco-2 ... Cell culture. The Caco-2 and HEK-293 cell lines used in the study were obtained from Highveld Biologicals (Pty) Ltd., ... Cell numbers (percent of control) for the Caco-2 and HEK-293 cells are shown for each oil concentration. The assays were ...

In vitro cell culture. The colon-derived human epithelial cell lines T84, HT-29, and Caco-2 were grown in a 1:1 mixture of ... ELISA. Expression of epithelial cell surface proteins was evaluated by use of a cell surface ELISA, as previously described on ... Extracts from T84d cells were used as a positive control. Blots of the proteins immunoprecipitated from T84d cells with the D5 ... Intestinal heat shock protein 110 regulates expression of CD1d on intestinal epithelial cells. Sean P. Colgan,1 Richard S. ...

2012) Inclusion bodies as potential vehicles for recombinant protein delivery into epithelial cells. Microb Cell Fact, vol. 11 ... The Caco-2 cell culture model enables sensitive detection of enhanced protein permeability in the presence of N-decyl-[beta]-d- ... Bacterial cell disruption : a crucial step in protein production. New biotechnology, 2013, 30:250-254, doi: 10.1016/j.nbt. ... Microb Cell Fact 9, 66 [1-16].. *PETERNEL, Špela, LIOVIĆ, Mirjana (2010) Production of recombinant proteins in bacteria : the ...

By supporting tight junctions, or multi-protein cells that tightly bind the epithelial cells within the stomach lining, the ... the Intestinal Barrier by Facilitating Tight Junction Assembly via Activation of AMP-Activated Protein Kinase in Caco-2 Cell ... Butyric acid is an integral, short-chain fatty acid acting as an energy source for epithelial cells. This acid also supports ... The intestinal barrier is made up of a layer of epithelial cells, preventing harmful pathogens and toxins from entering the ...

Antiproliferative effects of conjugated linoleic acid on human colon adenocarcinoma cell line Caco-2. Asia Pacific Journal of ... Docosahexaenoic acid induces apoptosis in CYP2E1-containing HepG2 cells by activating the c-Jun Nterminal protein kinase ... Molecular mechanism of gossypol-induced cell growth inhibition and cell death of HT-29 human colon carcinoma cells. Biochemical ... Molecular mechanism of gossypol-induced cell growth inhibition and cell death of HT-29 human colon carcinoma cells. Biochemical ...

New research shows that humans may have difficulty in taking up certain proteins from plant-based products. ... shows how proteins from plant-based meat substitutes may not be as accessible to human cells as proteins from animal meat. ... using a permeation layer of Caco-2 cells that are found in the colon of the large intestine. Analysis through mass-spectrometry ... Animal protein - including beef, pork, poultry, fish, dairy products and eggs - all contain nine essential amino acids. Only ...