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*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... tumor cells as well as other cells.. Cancer Biology, Issue 55, tumor, vascular, endothelial, tube formation, Matrigel, in vitro ... Tumor Cells, Cultured, Early Detection of Cancer, senescence, cancer, cells, flow cytometry, C12FDG, cell culture, clinical ... Interaction of HA with its cell surface receptor CD44 induces signaling events that promote tumor cell growth, survival, and ... Human cells do not indefinitely proliferate. Upon external and/or intrinsic cues, cells might die or enter a stable cell cycle ...
  https://www.jove.com/visualize/abstract/23028492/salinomycin-induces-autophagy-colon-breast-cancer-cells-with
*  Patent US6087157 - Device and method for analyzing tumor cell invasion of an extracellular matrix - Google Patents
Methods for culturing eukaryotic cells and studying their growth characteristics, including the invasive growth characteristics ... of tumor cells, on the submucosal matrix are described. ... A device for analyzing the in-vitro growth properties of cells ... inexpensive cell culture growth substrate that promotes or induces growth and differentiation of cells cultured in vitro. ... certain cell types, such as fetal rat cells, when seeded on the luminal side proliferate to form a polylayer of cells. Cells of ...
  http://www.google.com/patents/US6087157?dq=6,232,546
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... and cultured with IL-7 + IL-15 for additional 4 days. Tumor-specificity and anti-tumor efficacy of the ex vivo expanded T cells ... proteins by cells on scaffolds is described. Culturing cells on scaffolds in vitro can improve scaffold strength and elasticity ... Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies. Authors: Enza Lonardo, Michele ... their more differentiated progenies do not survive and proliferate during the initial phase following seeding of single cells. ...
  https://www.jove.com/visualize/abstract/26086247/uncoupling-elastin-complex-receptor-during-vitro-aging-is-related-to
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... and found that gene-modified T cells mediated targeted killing of HER2-positive tumor cells in vitro and in vivo in an ... and number of tumor spheres formed represents CSC activity harbored in either bulk populations of cultured cancer cells or ... Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies. Authors: Enza Lonardo, Michele ... administration of the peptide vaccine induces a Th1-polarized serum cytokine profile and a MUC1 specific T-cell response. All ...
  https://www.jove.com/visualize/abstract/25680114/the-prognostic-role-mtor-p-mtor-for-survival-non-small-cell-lung
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... and number of tumor spheres formed represents CSC activity harbored in either bulk populations of cultured cancer cells or ... Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies. Authors: Enza Lonardo, Michele ... The in vitro smooth muscle contractility method has been used extensively for over 50 years, and has provided data that ... their more differentiated progenies do not survive and proliferate during the initial phase following seeding of single cells. ...
  https://www.jove.com/visualize/abstract/25785738/differences-energy-balance-related-behaviours-european-preschool
*  Chloramphenicol Induces Abnormal Differentiation and Inhibits Apoptosis in Activated T Cells | Cancer Research
... both of which are derived from proliferating cells. Because our preliminary experiments with in vitro culture of mouse ... phase among cultured and clinical tumor cells]. Zhonghua Zhong Liu Za Zhi 2002; 24: 215-8. ... Cellular morphologic characteristics were examined under light microscopy. For immunofluorescence staining, freshly cultured ... Cell staining. For morphologic study, cell smears were prepared by spreading of cultured cells on a glass slide and allowing ...
  http://cancerres.aacrjournals.org/content/68/12/4875
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... and cultured with IL-7 + IL-15 for additional 4 days. Tumor-specificity and anti-tumor efficacy of the ex vivo expanded T cells ... We have recently developed a 3D in vitro system to model the in vivo growth characteristics of cells that exhibit either ... and can distinguish between metastatic and non-metastatic tumor clones; and 3) curative tumor resection induces dynamic changes ... a high throughput manner mechanistic insights into what triggers the emergence of solitary dormant tumor cells to proliferate ...
  https://www.jove.com/visualize/abstract/19557135/a-systems-immunology-approach-to-host-tumor-interaction-large-scale
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... tumor cells as well as other cells.. Cancer Biology, Issue 55, tumor, vascular, endothelial, tube formation, Matrigel, in vitro ... We have recently developed a 3D in vitro system to model the in vivo growth characteristics of cells that exhibit either ... We demonstrated that tumor cells that exhibit dormancy in vivo at a metastatic site remain quiescent when cultured in a 3- ... a high throughput manner mechanistic insights into what triggers the emergence of solitary dormant tumor cells to proliferate ...
  https://www.jove.com/visualize/abstract/22279592/erbb2-ephrinb1-src-kinase-ptpn13-signaling-complex-regulates-map
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
While these cultured tumor stem cells were relatively small, often bipoar with high nuclear to cytoplasmic ratio when cultured ... We have recently developed a 3D in vitro system to model the in vivo growth characteristics of cells that exhibit either ... a high throughput manner mechanistic insights into what triggers the emergence of solitary dormant tumor cells to proliferate ... administration of the peptide vaccine induces a Th1-polarized serum cytokine profile and a MUC1 specific T-cell response. All ...
  https://www.jove.com/visualize/abstract/25202965/quantitative-apparent-diffusion-coefficient-measurements-obtained-3
*  Autophagy Is Activated in Colorectal Cancer Cells and Contributes to the Tolerance to Nutrient Deprivation | Cancer Research
Proliferating cancer cells require nutrients for growth. Tumor angiogenesis is one way to increase the blood flow to supply the ... Cells were cultured for 48 h before analysis.. Cell death assay. Cell viability was assessed by the Hoechst 33342 (Sigma) ... Taken together, we conclude that active autophagy contributes to tumor cell survival in colorectal cancers in vitro and in vivo ... 1A). We observed that glucose starvation also induces AMPKα phosphorylation in DLD-1 cells (data not shown), suggesting that ...
  http://cancerres.aacrjournals.org/content/67/20/9677
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
BD BioCoat Tumor Invasion System provides cells with conditions that allow assessment of their invasive property in vitro1,2. ... We confirmed that S100A6 induces PDAC cell migration and invasion via activation of ?-catenin in vitro. Assessment of mRNA and ... and number of tumor spheres formed represents CSC activity harbored in either bulk populations of cultured cancer cells or ... Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies. Authors: Enza Lonardo, Michele ...
  https://www.jove.com/visualize/abstract/25799022/s100-calcium-binding-protein-a6-promotes-epithelial-mesenchymal
*  Tumor-Specific CD8+ T Cells Expressing Interleukin-12 Eradicate Established Cancers in Lymphodepleted Hosts | Cancer Research
... we adoptively transferred decreasing doses of the gene-modified T cells cultured in vitro for 5 to 7 days into sublethally ... 3A). We next quantified the characteristics and numbers of tumor-infiltrating cells and found that mice treated with pmel-1IL- ... Interleukin-12 therapy of cutaneous T-cell lymphoma induces lesion regression and cytotoxic T-cell responses. Blood 1999;94:902 ... failed to proliferate on secondary stimulation (Supplementary Fig. S4), and exhibited a small decrease in cytotoxic ability ( ...
  http://cancerres.aacrjournals.org/content/70/17/6725
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... and number of tumor spheres formed represents CSC activity harbored in either bulk populations of cultured cancer cells or ... Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies. Authors: Enza Lonardo, Michele ... here we describe in vitro screening methods that utilizes the mouse BAF3 cells expressing the random mutation library of JAK2 ... their more differentiated progenies do not survive and proliferate during the initial phase following seeding of single cells. ...
  https://www.jove.com/visualize/abstract/26087013/clonal-evolution-blast-crisis-correlate-with-enhanced-proteolytic
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Tumor Cells, Cultured, Early Detection of Cancer, senescence, cancer, cells, flow cytometry, C12FDG, cell culture, clinical ... Human cells do not indefinitely proliferate. Upon external and/or intrinsic cues, cells might die or enter a stable cell cycle ... Stem Cell Transplantation in an in vitro Simulated Ischemia/Reperfusion Model. Authors: Attila Cselenyák, Zsolt Benko, Mónika ... High glucose induces bone marrow-derived mesenchymal stem cell senescence by upregulating autophagy. ...
  https://www.jove.com/visualize/abstract/25961745/high-glucose-induces-bone-marrow-derived-mesenchymal-stem-cell
*  T Helper 1 (Th1) and Th2 Characteristics Start to Develop During T Cell Priming and Are Associated with an Immediate Ability to...
1 or Swiss type mouse mammary tumor virus [MMTV(SW)] ((26)). MMTV(SW) induces both a polyclonal and envelope-specific ... this section could not be used to count proliferating T cells as BrdU+ B cells in the T zone surrounded by CD3+ cells can not ... This correlates with findings from studies in vitro, where signals from T cells like CD40 ligation on B cells can induce Ig ... 45)) reported that naïve T cells cultured with Con A initially upregulate both IFN-γ and IL-4 message, but when IL-4 is present ...
  http://jem.rupress.org/content/187/8/1193
*  Interleukin-24 Induces Expression of β4 Integrin but Suppresses Anchorage-Independent Growth of Rat Mammary Tumor Cells by a...
2A ). FE1.3 cells express high levels of IL-24, and we showed that medium in which these cells had been cultured also ... IL-24 Inhibits Cell Migration and Invasion In vitro. As illustrated in Fig. 3B, FE1.2 cells infected with the retrovirus ... Retroviral expression of IL-24 in FE1.2 cells reduced the ability of these cells to proliferate in monolayer culture compared ... of IL-24 in our anchorage-dependent cells suggested a role for this cytokine in determining their growth characteristics. For ...
  http://mcr.aacrjournals.org/content/7/3/433
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Applying a direct current electric field to cultured cells in vitro induces directional cell migration, or galvanotaxis. The 2- ... Fibroblasts proliferate very efficiently in culture and in mixed cell populations these cells may overrun myogenic cells to ... This highly lethal disease stage is associated with the presence of circulating tumor cells (CTCs). These rare cells have been ... Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies. Authors: Enza Lonardo, Michele ...
  https://www.jove.com/visualize/abstract/26024527/cell-kinetic-studies-fail-to-identify-sequentially-proliferating
*  TAZ Promotes Cell Proliferation and Epithelial-Mesenchymal Transition and Is Inhibited by the Hippo Pathway | Molecular and...
TAZ induces the EMT of MCF10A cells. (A) TAZ induces a morphology change in MCF10A cells. Phase-contrast images of MCF10A cells ... Mer is a tumor suppressor, and Sav and Mob mutation have also been reported in tumor cell lines (14, 31). Therefore, the ... Cell culture and transfection.293T, HeLa, and BOSC cells were cultured in Dulbecco's modified Eagle's medium (DMEM) (Invitrogen ... Both the MCF10A/TAZ and MCF10A/TAZ4SA cells proliferated significantly faster than the vector control cells (Fig. 4A). ...
  https://mcb.asm.org/content/28/7/2426?ijkey=a297ee2cf376642df3ea2f51112c2fe331272b7f&keytype2=tf_ipsecsha
*  Acquisition of Neuroendocrine Characteristics by Prostate Tumor Cells Is Reversible | Cancer Research
Although NE cells are nonmitotic, proliferating cells are found in direct proximity to them, suggesting that NE cells provide ... Cells were cultured for 7 days in T-media + 5% FBS (untreated cells; A) or in the presence of 10 μm Fsk (B) or 10 μm Epi (C) ... In vitro, differentiation of the LNCaP and PC3M prostatic tumor cell lines to a NE phenotype can be induced by dibutyryl cyclic ... Induction of NE Differentiation of LNCaP Induces Reversible Changes in the Expression of NE Markers.. LNCaP and C4-2 cells were ...
  http://cancerres.aacrjournals.org/content/59/15/3821
*  Mammary-specific inactivation of E-cadherin and p53 impairs functional gland development and leads to pleomorphic invasive...
Δ/Δ tumor cells were not able to survive, whereas mILC cells readily survived and proliferated in an anchorage-independent ... B) Loss of E-cadherin induces anoikis resistance in the absence of p53. Tumor cell lines established from Wcre;Trp53F/F (AC) ... From the primary tumors that developed in our mouse model, we isolated tumor cells, which were then cultured and subsequently ... In conclusion, in vitro anoikis resistance is a reliable prognosticator of in vivo metastatic dissemination, using cell lines ...
  http://dmm.biologists.org/content/4/3/347
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... and number of tumor spheres formed represents CSC activity harbored in either bulk populations of cultured cancer cells or ... Modeling Astrocytoma Pathogenesis In Vitro and In Vivo Using Cortical Astrocytes or Neural Stem Cells from Conditional, ... Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies. Authors: Enza Lonardo, Michele ... administration of the peptide vaccine induces a Th1-polarized serum cytokine profile and a MUC1 specific T-cell response. All ...
  https://www.jove.com/visualize/abstract/26034456/myopericytoma-of-the-distal-forearm-a-case-report
*  A Monoclonal Antibody Reactive with a 40-kDa Molecule on Fetal Thymocytes and Tumor Cells Blocks Proliferation and Stimulates...
Characteristics in vivo and in vitro of tumor cells selected for resistance to syngeneic lymphocyte-mediated cytotoxicity. ... In contrast, E710.2.3 proliferates spontaneously when cultured at high density in the absence of PMA or other cells. When ... DMF10.62.3 induces cell death by apoptosis. On visual observation of cultures of cells treated with Ab DMF10.62.3 the number of ... E710.2.3 was chosen for this analysis because in vitro it is stimulated to proliferate upon contact with normal cells such as ...
  http://www.jimmunol.org/content/163/3/1306
*  Protocols and Video Articles Authored by Lijun Liu
A Model for Perineural Invasion in Head and Neck Squamous Cell Carcinoma' ... Isolation and Culture of Adult Mouse Cardiomyocytes for Cell Signaling and in vitro Cardiac Hypertrophy', ' ... Our results indicate that myopodin inhibits tumor growth and invasion both in vitro and in vivo. The activity of tumor ... The MiR-7 Identified from Collagen Biomaterial-based Three-dimensional Cultured Cells Regulates Neural Stem Cell ...
  https://www.jove.com/author/Lijun_Liu
*  Plus it
... that proliferating fibroblasts in the tumor microenvironment generate fibrotic foci and increase the invasive characteristics ... Fibroblasts cultured from malignant tumors have stimulatory effects on MCF-7 cells, whereas fibroblasts cultured from normal ... Tumor Stromal Fibroblasts: Contracted Farmers. Both in vivo and in vitro studies have demonstrated that fibroblasts contribute ... Amphibian tail resorption in tadpoles induces a set of complex developmental changes similar to those seen with tumor invasion ...
  http://clincancerres.aacrjournals.org/content/9/5/1590
*  Most recent papers with the keyword Acidification | Read by QxMD
Beating stem cell-derived cardiomyocytes are a preferred in vitro model to further uncover the mechanisms of DOX-induced ... Physakengose G induces apoptosis via EGFR/mTOR signaling and inhibits autophagic flux in human osteosarcoma cells. ... Our objective was to use cultured induced-pluripotent stem cell(iPSC)-derived mouse cardiomyocytes (Cor.At) to investigate the ... effects of DOX on cell and mitochondrial metabolism, as well as on stress responses. Non-proliferating and beating Cor... ...
  https://www.readbyqxmd.com/keyword/84111