Loading...
*  Aqueous normal-phase chromatography - Wikipedia
Retention decreases as the amount of polar solvent in the mobile phase increases. In reversed phase chromatography, the most ... Typical stationary phases for normal-phase chromatography are silica or organic moieties with cyano and amino functional groups ... In normal-phase chromatography, the stationary phase is polar and the mobile phase is nonpolar. In reversed phase we have just ... Retention increases as the amount of the polar solvent (water) in the mobile phase increases. Normal phase chromatography, an ...
  https://en.wikipedia.org/wiki/Aqueous_normal-phase_chromatography
*  Reversed-Phase SPE Methodology in Solid Phase Extraction | China-Mainland | Sigma-Aldrich
Reversed-phase SPE is considered the least selective retention mechanism when compared to normalphase or ion-exchange SPE. ... Reversed-Phase SPE Methodology in Solid Phase Extraction. ... organic solvent or solvent combination of sufficient non-polar ... Aqueous Sample Matrix/Mobile Phase Environment. Reversed-phase SPE is considered the least selective retention mechanism when ... introducing it to the SPE phase.. *Conditioning/Equilibration Conditioning wets or activates the bonded phases to ensure ...
  https://www.sigmaaldrich.com/china-mainland/zh/analytical-chromatography/sample-preparation/spe/reversedphase-methodology.html
*  Reversed-phase chromatography - Wikipedia
Hydrophobic molecules can be eluted from the column by decreasing the polarity of the mobile phase using an organic (non-polar ... since the polarity of the mobile and stationary phases have been inverted - hence the term reversed-phase chromatography. ... Mixtures of water or aqueous buffers and organic solvents are used to elute analytes from a reversed-phase column. The solvents ... Reversed-phase chromatography employs a polar (aqueous) mobile phase. As a result, hydrophobic molecules in the polar mobile ...
  https://en.wikipedia.org/wiki/Reversed-phase_chromatography
*  Hydrophilic interaction chromatography - Wikipedia
HILIC can offer a ten fold increase in sensitivity over reversed-phase chromatography because the organic solvent is much more ... phases amide bonded phases cationic bonded phases zwitterionic bonded phases A typical mobile phase for HILIC chromatography ... the mobile phase forms a water-rich layer on the surface of the polar stationary phase vs. the water-deficient mobile phase, ... which are soluble in high-organic solvent mixtures (ca. 70%-90% acetonitrile), can be used to increase the mobile phase ...
  https://en.wikipedia.org/wiki/Hydrophilic_interaction_chromatography
*  High-performance liquid chromatography - Wikipedia
Reversed phase HPLC (RP-HPLC) has a non-polar stationary phase and an aqueous, moderately polar mobile phase. One common ... Common mobile phases used include any miscible combination of water with various organic solvents (the most common are ... In reversed-phase chromatography, solvent A is often water or an aqueous buffer, while B is an organic solvent miscible with ... is a chromatographic technique which encompasses the mobile phase region between reversed-phase chromatography (RP) and organic ...
  https://en.wikipedia.org/wiki/High-performance_liquid_chromatography
*  Hydrophilic Interaction Chromatography for the Characterization of Therapeutic Monoclonal Antibodies at Protein, Peptide, and...
A good example is hydrophilic interaction chromatography (HILIC). While the technique has been used for more than 20 years to ... A good example is hydrophilic interaction chromatography (HILIC). While the technique has been used for more than 20 years to ... in combination with an aqueous organic solvent as mobile phase (12). The latter should contain more than 60-70% of an organic ... Besides the analysis of polar compounds, HILIC can also be considered as a complementary technique to reversed-phase LC for the ...
  http://www.chromatographyonline.com/hydrophilic-interaction-chromatography-characterization-therapeutic-monoclonal-antibodies-protein-pe
*  Biotage - Search Literature
Commonly used reversed-phase solvents typically include water with an organic solvent such as methanol or acetonitrile - each ... Flash Chromatography: Using UV Detection with UV Absorbing Mobile Phases Part No: AN092.v1 Issued year: 2014 File size: 1.12mb ... Reversed-phase chromatography is typically used when you need to separate several milligrams of relatively polar compounds that ... If you are currently using reversed-phase at preparative scale, such as flash chromatography, you know the mobile phase ...
  http://www.biotage.com/literature/search?q=&display_type=1&document_search%5Bproduct_page_ids%5D%5B%5D=201
*  Biotage - Search Literature
Commonly used reversed-phase solvents typically include water with an organic solvent such as methanol or acetonitrile - each ... Flash Chromatography: Using UV Detection with UV Absorbing Mobile Phases Part No: AN092.v1 Issued year: 2014 File size: 1.12mb ... Because polar solvents cause poor chromatographic results when used as injection solvents in normal-phase flash chromatography ... If you are currently using reversed-phase at preparative scale, such as flash chromatography, you know the mobile phase ...
  http://www.biotage.com/literature/search?q=&display_type=1&document_search%5Bproduct_page_ids%5D%5B%5D=204
*  Biotage - Biotage ZIP® Sphere
Impact of Injection Solvent Choice on Peak Shape and Resolution in Reversed-phase Flash Chromatography Reversed-phase ... Flash Chromatography: Using UV Detection with UV Absorbing Mobile Phases The UV absorption spectrum of some solvents overlaps ... Acetonitrile as a Replacement for Methanol in Normal-phase Flash Chromatography When it comes to polar organic compound ... Greener reversed-phase Flash Chromatography Using Acetone Instead of Acetonitrile As reversed-phase flash chromatography gains ...
  http://biotage.com/product-page/biotage-zip-sphere
*  Hydrophobicity scales - Wikipedia
However, organic solvents are slightly miscible with water and the characteristics of both phases change making it difficult to ... Reversed phase liquid chromatography (RPLC) is the most important chromatographic method for measuring solute hydrophobicity. ... Vapor phases represent the simplest non polar phases, because it has no interaction with the solute. The hydration potential ... 1-butanol and pyridine were used as the mobile phase in this particular scale and glycine was used as the reference value. ...
  https://en.wikipedia.org/wiki/Hydrophobicity_scales
*  solvent separation Equipment available in Bolivia | Environmental XPRT
Results for solvent separation equipment from Airdot, AMBERLITE, Analtech and other leading brands. Compare and contact a ... SFC-Amino phase is compatible with versatile mobile phases from non-aqueous solvents, such as hexane/ethyl acetate and ... ES Industries manufactures Polar Bonded materials for Normal Phase HPLC that are compatible with most organic solvents. These ... Prepsolv - Solvents optimized for preparative Chromatography. Prepsolv® solvents are tailored to the requirements of ...
  https://www.environmental-expert.com/products/keyword-solvent-separation-11524/available-in-bolivia?redirectedToServing=1
*  Column Technology for Achiral SFC Separations - Aug 23 2018 - Matthew Przybyciel - Life Science News Articles - Labmate Online
Supercritical fluid chromatography (SFC) is a powerful tool for the separation of both chiral [1-3] and Achiral [4, 5] ... Mobile phases used in normal phase liquid chromatography are composed of non-polar solvents, such as hexane which can be ... In reversed phase HPLC, C18 is viewed as the 'universal' stationary phase; however, the analogous SFC phase is currently ... organic solvent modifiers must be mixed into the CO2 mobile phase stream employing a second high pressure HPLC pump. While ...
  https://www.labmate-online.com/article/chromatography/1/es-industries/column-technology-for-achiral-sfc-separations/2415
*  Mourne Training Services: 2009
The organic components in the mixture are attracted to both the non-polar stationary phase and also the mobile phase in which ... this organic solvent rinse was repeated between injections to ensure that the chromatography was acceptable. The reason for ... above except that the stationary phase is polar and the mobile phase is non-polar and therefore the elution order is reversed. ... Normal phase partition HPLC (using polar bonded phases) is most suitable for polar components whereas normal phase adsorption ...
  http://blog.mournetrainingservices.co.uk/2009/
*  Tetrahydrofuran - Wikipedia
THF is used as a component in mobile phases for reversed-phase liquid chromatography. It has a greater elution strength than ... It is an aprotic solvent with a dielectric constant of 7.6. It is a moderately polar solvent and can dissolve a wide range of ... "Drying of Organic Solvents: Quantitative Evaluation of the Efficiency of Several Desiccants". Journal of Organic Chemistry. 75 ... Being polar and having a wide liquid range, THF is a versatile solvent. About 200,000 tonnes of tetrahydrofuran are produced ...
  https://en.wikipedia.org/wiki/Tetrahydrofuran
*  Biotage - Biotage® SNAP Ultra C18
SNAP Ultra C18 are reversed phase cartridges that can load 2-3% sample and enables really fast, cost effecient and sharp peak ... Reversed Phase: Highly Effective for Polar Molecule Purifications. Unlike normal-phase chromatography, reversed-phase uses a ... and a mobile phase (elution solvent mixture) to separate and purify a mixture of organic compounds.. Tags: Brochures ... Investigating the use of Mixed Stationary Phases for Peptide Purification via Reversed Phase Flash Chromatography Peptide ...
  https://biotage.com/product-page/biotage-snap-ultra-c18
*  SUPELCOSIL™ LC-Si HPLC Column 5 μm particle size, L × I.D. 25 cm × 21.2 mm | Sigma-Aldrich
A typical mobile phase used with normal-phase silica columns consists of a hydrocarbon solvent such as hexane or heptane, mixed ... LC-Si silica, Polar Bonded Phases, Preparative Scale SUPELCOSIL Columns, SUPELCOSIL HPLC Columns, SUPELCOSIL Semi-Prep and ... is frequently used in preparative chromatography due to its low operating cost and back pressure compared to reversed-phase ... Silica is particularly popular among organic chemists as a low-pressure preparative tool. The unmodified silica in SUPELCOSIL ...
  https://www.sigmaaldrich.com/catalog/product/supelco/54843?lang=en®ion=US
*  IRJPAS
... and thus not adsorption chromatography. When the mobile phase contains , 60% organic solvent, then hydrophilic interaction ... provides an alternative approach to effectively separate small polar compounds on polar stationary phases. Hydrophilic ... of partition chromatography which occupies the opposite end of the partition spectrum from reversed phase liquid chromatography ... one of the phases being mobile and the other stationary. The stationary phase is a solid or a liquid, and the mobile phase is a ...
  http://irjpas.com/view_content.php?quat=3&date=Volume-3:Issue-2%20
*  Lecture 8: Protein purification - ppt video online download
The mixture of substances (proteins) to be fractionated is dissolved in a liquid or gaseous fluid called the mobile phase. This ... These are sometimes called resins when used in liquid chromatography. The stationary phase has certain physical and chemical ... Common types of chromatographic stationary phases Ion exchange Hydrophobic Gel filtration Affinity ... solution is passed through a column consisting of a porous solid matrix called the stationary phase. ...
  http://slideplayer.com/slide/4335435/
*  Effects of organic modifier and temperature on the enantiomeric separation of several azole drugs using supercritical fluid...
... using supercritical fluid chromatography with the Chiralpak AD column, have been investigated in this work. For this purpose ... four different organic modifiers (methanol, ethanol, 2-propanol and acetonitrile) were evaluated. Only in the case of two ... The effects of organic modifier and temperature on the enantioseparation of 10 triazoles and eight imidazoles, ... Reversed-phase screening strategies for liquid chromatography on polysaccharide-derived chiral stationary phases.. *Tong Zhang ...
  https://www.semanticscholar.org/paper/Effects-of-organic-modifier-and-temperature-on-the-Toribio-Bernal/e2531f7aa43153deef9b8225af47bb8d99e889db
*  4 Gas Chromatography/Gas Chromatography Mass Spectrometry - Tài liệu text
the mobile phase will increase retention times of nonpolar components and decrease those of polar. Adding an organic solvent ... Reverse-phase HPLC uses a nonpolar stationary phase and a polar mobile phase to achieve separation (the reverse of. normal ... The sample is agitated and the phases are allowed to separate.. • A sample of the organic liquid is drawn from the vial and ... Normal-phase HPLC uses a polar stationary phase and a nonpolar mobile phase to achieve separation. Columns are. packed with ...
  https://toc.123doc.org/document/2650956-4-gas-chromatography-gas-chromatography-mass-spectrometry.htm
*  Thin-layer chromatography - Wikipedia
... when the stationary phase is polar and the mobile phase is non-polar, the method is normal-phase as opposed to reverse-phase. ... These values depend on the solvent used and the type of TLC plate and are not physical constants. In organic chemistry, ... For example, with silica gel, a very polar substance, non-polar mobile phases such as heptane are used. The mobile phase may be ... If the mobile phase is changed to a more polar solvent or mixture of solvents, it becomes better at binding to the polar plate ...
  https://en.wikipedia.org/wiki/Thin-layer_chromatography
*  Micellar liquid chromatography - Wikipedia
... and a polar mobile or liquid phase. The mobile phase generally consists of an aqueous portion with an organic addition, such as ... "Chromatographic behaviour in reversed-phase high-performance liquid chromatography with micellar and submicellar mobile phases ... A small amount of organic solvent is often added to the mobile phase to help improve efficiency and to improve separations of ... Reverse phase high-performance liquid chromatography (RP-HPLC) involves a non-polar stationary phase, often a hydrocarbon chain ...
  https://en.wikipedia.org/wiki/Micellar_liquid_chromatography
*  Chromatography - Wikipedia
In the case of HPLC the mobile phase consists of a non-polar solvent(s) such as hexane in normal phase or a polar solvent such ... Reversed-phase chromatography (RPC) is any liquid chromatography procedure in which the mobile phase is significantly more ... Countercurrent chromatography (CCC) is a type of liquid-liquid chromatography, where both the stationary and mobile phases are ... Purnell equation Van Deemter equation Weak affinity chromatography McMurry, John (2011). Organic chemistry: with biological ...
  https://en.wikipedia.org/wiki/Chromatography
*  Countercurrent chromatography - Wikipedia
In reversed-phase (e.g. aqueous mobile phase) elution, the aqueous phase is used as the mobile phase with a less polar ... as the mobile phase at a certain point the organic phase is pumped through the column which effectively pushes out both phases ... In countercurrent chromatography the same solvent system may be used in either normal or reversed phase mode simply by ... phase chromatography, and the non-polar stationary phase chromatography became "reversed" phase chromatography. ...
  https://en.wikipedia.org/wiki/Countercurrent_chromatography
*  Superheated water - Wikipedia
Reverse phased HPLC often uses methanol / water mixtures as the mobile phase. Since the polarity of water spans the same range ... Water becomes less polar and behaves more like an organic solvent such as methanol or ethanol. Solubility of organic materials ... a clean eluent for reverse phase high performance chromatography". Analytical Communications. Royal Society of Chemistry. 33 (9 ... The maximum temperature is limited to that at which the stationary phase is stable. C18 bonded phases which are common in HPLC ...
  https://en.wikipedia.org/wiki/Superheated_water